Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001530

Physical Location & Seq

Genomic Coordinates Reverse (-)

868345 .. 868767

Gene Structure 423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1013600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 423 bp

ATGGAAAAAGTAGCACAATACGAGCGCGTTTTCAAGCAATTTGATGGCAACGGAGACAATAAGATATCACCTATGGAGCTGCAACAATGTGTCGGAGCGATAGGCGGCGAGCTCTCGCTGACAGAGGCGGAGGTGGCGGTGGAGTACCTAGACTCAGACGGCGACGGGCTGCTGGGGTTGGATGATTTTGTCAAGTTTGTCGACGGAGGATGCGACGAAGAGAAGGTGTGTGTTTTGAGGGAGGCTTTTAAGATGTATGTGATGGACGGGAGTGGGTGCATTACGCCCAAGAGTCTTAAGAGGATGCTGAGTCGACTCGGCGAGTCCAAGAGCGTCGACGAGTGCAAGAACATGATTGCGAGGTTTGATCTCAACGGTGATGGGGTGCTCAATTTTGATGAGTTTAAGGTTATGATGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0008150 GO:0009611 GO:0016020 GO:0043167 GO:0043169 GO:0044464 GO:0046872 GO:0050896 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

141

Amino Acids

15.55

Weight (kDa)

4.42

Isoelectric Point (pI)

25.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	8 - 66	1.2e-10	EF-hand domain pair
EF-hand_5	PF13202	10 - 31	6.3e-07	EF hand
EF-hand_7	PF13499	78 - 139	4.9e-12	EF-hand domain pair
EF-hand_8	PF13833	91 - 140	7.5e-11	EF-hand domain pair
EF-hand_1	PF00036	114 - 139	2.2e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76640 AT1G76650 AT1G76650 AT1G76650
fragaria_vesca	FvH4_5g08060 FvH4_5g26790 FvH4_6g53150
malus_domestica	MD06G1143500.v1.1 MD09G1013600.v1.1 MD14G1158700.v1.1 MD17G1014200.v1.1
prunus_persica	Prupe.3G303600_v2.0.a1 Prupe.5G152700_v2.0.a1
pyrus_communis	pycom14g13240
rosa_chinensis	RchiOBHm_Chr2g0175201 RchiOBHm_Chr7g0190421 RchiOBHm_Chr7g0217701
rosa_laevigata	RLG00000002447 RLG00000002453 RLG00000004534 RLG00000022310
rosa_multiflora	Rmu_co8349129.1_g000001 Rmu_sc0000202.1_g000007 Rmu_sc0003920.1_g000012 Rmu_sc0010642.1_g000013 Rmu_sc0014718.1_g000001 Rmu_sc0017751.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077510 Rroxscaffold_3G00241780 Rroxscaffold_3G00264640
rosa_rugosa	Rorug02G0581300 Rorug06G0504700 Rorug07G0170500 Rorug07G0170700
rosa_samantha	Rh2AG662000 Rh2BG673200 Rh2BG674000 Rh2CG636400 Rh2DG687100 Rh7AG110400 Rh7AG311100 Rh7BG112900 Rh7BG302000 Rh7BG303000 Rh7CG115200 Rh7CG330100 Rh7DG113500 Rh7DG310900
rosa_wichuraiana	Rw2G054290 Rw7G009580 Rw7G026410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	201, 313, 336
AccII	CGCG	1 cut(s)	27
AciI	CCGC	3 cut(s)	105, 128, 137
AfaI	GTAC	1 cut(s)	146
AflII	CTTAAG	1 cut(s)	296
AgsI	TTSAA	1 cut(s)	34
AluBI	AGCT	2 cut(s)	79, 112
AluI	AGCT	2 cut(s)	79, 112
Alw21I	GWGCWC	2 cut(s)	114, 390
Alw26I	GTCTC	1 cut(s)	48
ApeKI	GCWGC	2 cut(s)	79, 169
AspLEI	GCGC	1 cut(s)	27
AsuHPI	GGTGA	2 cut(s)	60, 389
BanII	GRGCYC	1 cut(s)	114
Bbv12I	GWGCWC	2 cut(s)	114, 390
BbvI	GCAGC	2 cut(s)	66, 156
BccI	CCATC	3 cut(s)	38, 256, 374
BceAI	ACGGC	1 cut(s)	175
BcoDI	GTCTC	1 cut(s)	48
BfaI	CTAG	1 cut(s)	149
BfrI	CTTAAG	1 cut(s)	296
BisI	GCNGC	3 cut(s)	80, 106, 170
BlsI	GCNGC	3 cut(s)	81, 107, 171
BmsI	GCATC	2 cut(s)	200, 294
BseGI	GGATG	3 cut(s)	187, 215, 309
BseMII	CTCAG	2 cut(s)	168, 299
BseXI	GCAGC	2 cut(s)	66, 156
BseYI	CCCAGC	1 cut(s)	172
Bsh1236I	CGCG	1 cut(s)	27
BsiHKAI	GWGCWC	2 cut(s)	114, 390
BsmAI	GTCTC	1 cut(s)	48
Bsp1286I	GDGCHC	2 cut(s)	114, 390
Bsp143I	GATC	1 cut(s)	367
BspACI	CCGC	3 cut(s)	105, 128, 137
BspCNI	CTCAG	2 cut(s)	167, 300
BspFNI	CGCG	1 cut(s)	27
BspTI	CTTAAG	1 cut(s)	296
BssMI	GATC	1 cut(s)	367
Bst4CI	ACNGT	1 cut(s)	377
Bst6I	CTCTTC	1 cut(s)	213
BstAFI	CTTAAG	1 cut(s)	296
BstC8I	GCNNGC	1 cut(s)	110
BstDEI	CTNAG	2 cut(s)	154, 308
BstF5I	GGATG	3 cut(s)	187, 215, 309
BstFNI	CGCG	1 cut(s)	27
BstHHI	GCGC	1 cut(s)	27
BstKTI	GATC	1 cut(s)	370
BstMAI	GTCTC	1 cut(s)	48
BstMBI	GATC	1 cut(s)	367
BstMWI	GCNNNNNNNGC	1 cut(s)	134
BstUI	CGCG	1 cut(s)	27
BstV1I	GCAGC	2 cut(s)	66, 156
BtsCI	GGATG	3 cut(s)	187, 215, 309
Cac8I	GCNNGC	1 cut(s)	110
CfoI	GCGC	1 cut(s)	27
CseI	GACGC	1 cut(s)	322
Csp6I	GTAC	1 cut(s)	145
CviAII	CATG	1 cut(s)	352
CviJI	RGCY	4 cut(s)	79, 112, 169, 245
CviKI_1	RGCY	4 cut(s)	79, 112, 169, 245
CviQI	GTAC	1 cut(s)	145
DdeI	CTNAG	2 cut(s)	154, 308
DpnI	GATC	1 cut(s)	369
DpnII	GATC	1 cut(s)	367
Eam1104I	CTCTTC	1 cut(s)	213
EarI	CTCTTC	1 cut(s)	213
EciI	GGCGGA	1 cut(s)	143
Ecl136II	GAGCTC	1 cut(s)	112
Eco24I	GRGCYC	1 cut(s)	114
Eco32I	GATATC	1 cut(s)	66
Eco53kI	GAGCTC	1 cut(s)	112
EcoICRI	GAGCTC	1 cut(s)	112
EcoRV	GATATC	1 cut(s)	66
EcoT38I	GRGCYC	1 cut(s)	114
FaeI	CATG	1 cut(s)	355
FaiI	YATR	4 cut(s)	74, 258, 353, 413
FatI	CATG	1 cut(s)	351
FblI	GTMKAC	3 cut(s)	201, 313, 336
Fnu4HI	GCNGC	3 cut(s)	80, 106, 170
FokI	GGATG	3 cut(s)	194, 222, 316
FriOI	GRGCYC	1 cut(s)	114
Fsp4HI	GCNGC	3 cut(s)	80, 106, 170
FspBI	CTAG	1 cut(s)	149
GlaI	GCGC	1 cut(s)	26
GluI	GCNGC	3 cut(s)	80, 106, 170
GsaI	CCCAGC	1 cut(s)	176
HgaI	GACGC	1 cut(s)	322
HhaI	GCGC	1 cut(s)	27
Hin1II	CATG	1 cut(s)	355
Hin6I	GCGC	1 cut(s)	25
HinP1I	GCGC	1 cut(s)	25
HincII	GTYRAC	3 cut(s)	202, 314, 337
HindII	GTYRAC	3 cut(s)	202, 314, 337
HinfI	GANTC	5 cut(s)	152, 292, 310, 315, 323
HphI	GGTGA	2 cut(s)	60, 389
Hpy166II	GTNNAC	3 cut(s)	202, 314, 337
Hpy188I	TCNGA	2 cut(s)	95, 157
Hpy8I	GTNNAC	3 cut(s)	202, 314, 337
Hpy99I	CGWCG	5 cut(s)	167, 206, 218, 338, 341
HpyAV	CCTTC	1 cut(s)	217
HpyCH4III	ACNGT	1 cut(s)	377
HpyCH4V	TGCA	3 cut(s)	82, 279, 345
HpyF10VI	GCNNNNNNNGC	1 cut(s)	134
HpyF3I	CTNAG	2 cut(s)	154, 308
Hsp92II	CATG	1 cut(s)	355
HspAI	GCGC	1 cut(s)	25
Kzo9I	GATC	1 cut(s)	367
LmnI	GCTCC	2 cut(s)	76, 95
LpnPI	CCDG	1 cut(s)	158
Lsp1109I	GCAGC	2 cut(s)	66, 156
LweI	GCATC	2 cut(s)	200, 294
MaeI	CTAG	1 cut(s)	149
MalI	GATC	1 cut(s)	369
MboI	GATC	1 cut(s)	367
MboII	GAAGA	1 cut(s)	230
MhlI	GDGCHC	2 cut(s)	114, 390
MluCI	AATT	2 cut(s)	38, 391
MlyI	GAGTC	5 cut(s)	146, 301, 309, 319, 332
MmeI	TCCRAC	2 cut(s)	73, 159
MnlI	CCTC	7 cut(s)	118, 124, 200, 231, 235, 294, 354
MseI	TTAA	3 cut(s)	249, 297, 405
MspCI	CTTAAG	1 cut(s)	296
MvnI	CGCG	1 cut(s)	27
MwoI	GCNNNNNNNGC	1 cut(s)	134
NdeII	GATC	1 cut(s)	367
NlaIII	CATG	1 cut(s)	355
NmeAIII	GCCGAG	1 cut(s)	297
PcsI	WCGNNNNNNNCGW	1 cut(s)	210
PkrI	GCNGC	3 cut(s)	81, 107, 171
PleI	GAGTC	5 cut(s)	146, 300, 309, 318, 331
PpsI	GAGTC	5 cut(s)	146, 300, 309, 318, 331
Psp124BI	GAGCTC	1 cut(s)	114
PspFI	CCCAGC	1 cut(s)	172
RsaI	GTAC	1 cut(s)	146
RsaNI	GTAC	1 cut(s)	145
SacI	GAGCTC	1 cut(s)	114
SalI	GTCGAC	3 cut(s)	200, 312, 335
SaqAI	TTAA	3 cut(s)	249, 297, 405
SatI	GCNGC	3 cut(s)	80, 106, 170
Sau3AI	GATC	1 cut(s)	367
SchI	GAGTC	5 cut(s)	146, 301, 309, 319, 332
SduI	GDGCHC	2 cut(s)	114, 390
SetI	ASST	8 cut(s)	73, 81, 114, 135, 150, 228, 365, 411
SfaNI	GCATC	2 cut(s)	200, 294
SgrDI	CGTCGACG	1 cut(s)	335
SmlI	CTYRAG	1 cut(s)	296
SmoI	CTYRAG	1 cut(s)	296
Sse9I	AATT	2 cut(s)	38, 391
SsiI	CCGC	3 cut(s)	105, 128, 137
SspMI	CTAG	1 cut(s)	149
SstI	GAGCTC	1 cut(s)	114
TaaI	ACNGT	1 cut(s)	377
TaqI	TCGA	3 cut(s)	201, 313, 336
TasI	AATT	2 cut(s)	38, 391
TauI	GCSGC	1 cut(s)	108
Tru1I	TTAA	3 cut(s)	249, 297, 405
Tru9I	TTAA	3 cut(s)	249, 297, 405
TseI	GCWGC	2 cut(s)	79, 169
TspGWI	ACGGA	2 cut(s)	66, 219
Vha464I	CTTAAG	1 cut(s)	296
XmiI	GTMKAC	3 cut(s)	201, 313, 336
XspI	CTAG	1 cut(s)	149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.