Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001530

Physical Location & Seq

Genomic Coordinates Reverse (-)

28710193 .. 28710615

Gene Structure 423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1143500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 423 bp

ATGGGAAGTCACAAAGGATACGAACGTGTTTTCAGATATTTTGACGAAGATGGTGACGGAAAGGTTTCGCCTGCGGAGCTAAGGCGCCGGCTAGGGTTAATGGGTGGAGAGCTGCTGCAGAATGAGGCGGAGGCGGCGGTGGAAATGCTGGACTCGGACGGAGACGGATTGCTGGGTTTGGAGGATCTTGTGGGGTTAATGGAAGGAGGGGAAGAGGAGGACAAGGTGAAGGGTTTAAGGGAGGCTTTTGAGTTGTATGATGTGGAGGGGTGTGGGTTTATTACACCCAGCAGCTTGAAGAGAATGCTGAGCAAGTTGGGTGAGTCCAAGTCCGTTGATGAGTGCAAGGTGATGATCCAACGGTTTGATTTGAATGGAGATGGGGTGATTTGCTTTGAAGAGTTCAGAATCATGATGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010193 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0046872 GO:0050896 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

141

Amino Acids

15.61

Weight (kDa)

4.45

Isoelectric Point (pI)

44.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	9 - 67	3.5e-10	EF-hand domain pair
EF-hand_5	PF13202	10 - 29	9.6e-06	EF hand
AIF-1	PF21008	73 - 139	4.4e-06	Allograft inflammatory factor 1
EF-hand_7	PF13499	78 - 140	1.9e-13	EF-hand domain pair
EF-hand_8	PF13833	94 - 140	2.1e-08	EF-hand domain pair
EF-hand_1	PF00036	114 - 140	1.8e-07	EF hand domain
EF-hand_5	PF13202	119 - 139	4.9e-06	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0001530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76640 AT1G76650 AT1G76650 AT1G76650
fragaria_vesca	FvH4_5g08060 FvH4_5g26790 FvH4_6g53150
malus_domestica	MD06G1143500.v1.1 MD09G1013600.v1.1 MD14G1158700.v1.1 MD17G1014200.v1.1
prunus_persica	Prupe.3G303600_v2.0.a1 Prupe.5G152700_v2.0.a1
pyrus_communis	pycom14g13240
rosa_chinensis	RchiOBHm_Chr2g0175201 RchiOBHm_Chr7g0190421 RchiOBHm_Chr7g0217701
rosa_laevigata	RLG00000002447 RLG00000002453 RLG00000004534 RLG00000022310
rosa_multiflora	Rmu_co8349129.1_g000001 Rmu_sc0000202.1_g000007 Rmu_sc0003920.1_g000012 Rmu_sc0010642.1_g000013 Rmu_sc0014718.1_g000001 Rmu_sc0017751.1_g000002
rosa_roxburghii	Rroxscaffold_2G00077510 Rroxscaffold_3G00241780 Rroxscaffold_3G00264640
rosa_rugosa	Rorug02G0581300 Rorug06G0504700 Rorug07G0170500 Rorug07G0170700
rosa_samantha	Rh2AG662000 Rh2BG673200 Rh2BG674000 Rh2CG636400 Rh2DG687100 Rh7AG110400 Rh7AG311100 Rh7BG112900 Rh7BG302000 Rh7BG303000 Rh7CG115200 Rh7CG330100 Rh7DG113500 Rh7DG310900
rosa_wichuraiana	Rw2G054290 Rw7G009580 Rw7G026410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	84
AciI	CCGC	4 cut(s)	74, 128, 134, 137
AclWI	GGATC	2 cut(s)	192, 349
AcyI	GRCGYC	1 cut(s)	85
AflIII	ACRYGT	1 cut(s)	25
AgsI	TTSAA	3 cut(s)	298, 373, 398
AluBI	AGCT	3 cut(s)	79, 112, 294
AluI	AGCT	3 cut(s)	79, 112, 294
Alw26I	GTCTC	1 cut(s)	156
AlwI	GGATC	2 cut(s)	192, 349
ApeKI	GCWGC	3 cut(s)	112, 115, 291
Asp700I	GAANNNNTTC	1 cut(s)	64
AspLEI	GCGC	1 cut(s)	87
AsuHPI	GGTGA	5 cut(s)	65, 238, 332, 361, 397
BaeI	ACNNNNGTAYC	2 cut(s)	10, 43
BanI	GGYRCC	1 cut(s)	84
BbvI	GCAGC	3 cut(s)	99, 102, 303
BccI	CCATC	2 cut(s)	44, 374
BciVI	GTATCC	1 cut(s)	11
BcoDI	GTCTC	1 cut(s)	156
BfaI	CTAG	1 cut(s)	92
BfmI	CTRYAG	1 cut(s)	116
BfoI	RGCGCY	1 cut(s)	88
BfuI	GTATCC	1 cut(s)	11
BisI	GCNGC	4 cut(s)	113, 116, 135, 292
BlpI	GCTNAGC	1 cut(s)	308
BlsI	GCNGC	4 cut(s)	114, 117, 136, 293
BmiI	GGNNCC	1 cut(s)	86
BmsI	GCATC	1 cut(s)	405
Bpu10I	CCTNAGC	1 cut(s)	80
Bpu1102I	GCTNAGC	1 cut(s)	308
BsaHI	GRCGYC	1 cut(s)	85
BsaXI	ACNNNNNCTCC	2 cut(s)	369, 399
Bse118I	RCCGGY	1 cut(s)	87
BseMII	CTCAG	1 cut(s)	299
BseRI	GAGGAG	1 cut(s)	230
BseXI	GCAGC	3 cut(s)	99, 102, 303
BseYI	CCCAGC	2 cut(s)	172, 287
BshNI	GGYRCC	1 cut(s)	84
BsiSI	CCGG	1 cut(s)	88
BsmAI	GTCTC	1 cut(s)	156
BsmBI	CGTCTC	1 cut(s)	156
BsmI	GAATGC	1 cut(s)	309
Bsp143I	GATC	2 cut(s)	184, 354
Bsp1720I	GCTNAGC	1 cut(s)	308
BspACI	CCGC	4 cut(s)	74, 128, 134, 137
BspCNI	CTCAG	1 cut(s)	300
BspHI	TCATGA	1 cut(s)	411
BspLI	GGNNCC	1 cut(s)	86
BspMAI	CTGCAG	1 cut(s)	120
BspPI	GGATC	2 cut(s)	192, 349
BspT107I	GGYRCC	1 cut(s)	84
BsrFI	RCCGGY	1 cut(s)	87
BssAI	RCCGGY	1 cut(s)	87
BssMI	GATC	2 cut(s)	184, 354
BssNI	GRCGYC	1 cut(s)	85
Bst4CI	ACNGT	1 cut(s)	363
Bst6I	CTCTTC	3 cut(s)	207, 293, 393
BstACI	GRCGYC	1 cut(s)	85
BstC8I	GCNNGC	2 cut(s)	72, 89
BstDEI	CTNAG	2 cut(s)	80, 308
BstH2I	RGCGCY	1 cut(s)	88
BstHHI	GCGC	1 cut(s)	87
BstKTI	GATC	2 cut(s)	187, 357
BstMAI	GTCTC	1 cut(s)	156
BstMBI	GATC	2 cut(s)	184, 354
BstMWI	GCNNNNNNNGC	2 cut(s)	76, 134
BstSFI	CTRYAG	1 cut(s)	116
BstV1I	GCAGC	3 cut(s)	99, 102, 303
BstX2I	RGATCY	1 cut(s)	184
BstYI	RGATCY	1 cut(s)	184
BsuI	GTATCC	1 cut(s)	11
Cac8I	GCNNGC	2 cut(s)	72, 89
CciI	TCATGA	1 cut(s)	411
CfoI	GCGC	1 cut(s)	87
Cfr10I	RCCGGY	1 cut(s)	87
CviAII	CATG	1 cut(s)	412
CviJI	RGCY	5 cut(s)	79, 91, 112, 245, 294
CviKI_1	RGCY	5 cut(s)	79, 91, 112, 245, 294
DdeI	CTNAG	2 cut(s)	80, 308
DinI	GGCGCC	1 cut(s)	86
DpnI	GATC	2 cut(s)	186, 356
DpnII	GATC	2 cut(s)	184, 354
Eam1104I	CTCTTC	3 cut(s)	207, 293, 393
EarI	CTCTTC	3 cut(s)	207, 293, 393
EciI	GGCGGA	1 cut(s)	143
EgeI	GGCGCC	1 cut(s)	86
EheI	GGCGCC	1 cut(s)	86
Esp3I	CGTCTC	1 cut(s)	156
FaeI	CATG	1 cut(s)	415
FaiI	YATR	2 cut(s)	258, 413
FalI	AAGNNNNNCTT	2 cut(s)	229, 261
FatI	CATG	1 cut(s)	411
Fnu4HI	GCNGC	4 cut(s)	113, 116, 135, 292
Fsp4HI	GCNGC	4 cut(s)	113, 116, 135, 292
FspBI	CTAG	1 cut(s)	92
GlaI	GCGC	1 cut(s)	86
GluI	GCNGC	4 cut(s)	113, 116, 135, 292
GsaI	CCCAGC	2 cut(s)	176, 291
HaeII	RGCGCY	1 cut(s)	88
HapII	CCGG	1 cut(s)	88
HhaI	GCGC	1 cut(s)	87
Hin1I	GRCGYC	1 cut(s)	85
Hin1II	CATG	1 cut(s)	415
Hin6I	GCGC	1 cut(s)	85
HinP1I	GCGC	1 cut(s)	85
HinfI	GANTC	3 cut(s)	152, 323, 408
HpaII	CCGG	1 cut(s)	88
HphI	GGTGA	5 cut(s)	65, 238, 332, 361, 397
Hpy188I	TCNGA	3 cut(s)	35, 157, 407
Hpy188III	TCNNGA	1 cut(s)	412
HpyAV	CCTTC	2 cut(s)	197, 223
HpyCH4III	ACNGT	1 cut(s)	363
HpyCH4IV	ACGT	1 cut(s)	25
HpyCH4V	TGCA	3 cut(s)	118, 345, 418
HpyF10VI	GCNNNNNNNGC	2 cut(s)	76, 134
HpyF3I	CTNAG	2 cut(s)	80, 308
HpySE526I	ACGT	1 cut(s)	25
Hsp92I	GRCGYC	1 cut(s)	85
Hsp92II	CATG	1 cut(s)	415
HspAI	GCGC	1 cut(s)	85
KasI	GGCGCC	1 cut(s)	84
KroI	GCCGGC	1 cut(s)	87
KroNI	GCCGGC	1 cut(s)	89
Kzo9I	GATC	2 cut(s)	184, 354
LmnI	GCTCC	1 cut(s)	76
LpnPI	CCDG	5 cut(s)	84, 101, 134, 158, 301
Lsp1109I	GCAGC	3 cut(s)	99, 102, 303
LweI	GCATC	1 cut(s)	405
MaeI	CTAG	1 cut(s)	92
MaeII	ACGT	1 cut(s)	25
MaeIII	GTNAC	2 cut(s)	8, 53
MalI	GATC	2 cut(s)	186, 356
MboI	GATC	2 cut(s)	184, 354
MboII	GAAGA	4 cut(s)	59, 224, 310, 410
MflI	RGATCY	1 cut(s)	184
Mly113I	GGCGCC	1 cut(s)	85
MlyI	GAGTC	2 cut(s)	146, 332
MmeI	TCCRAC	1 cut(s)	382
MnlI	CCTC	8 cut(s)	118, 124, 175, 200, 208, 211, 235, 259
MroNI	GCCGGC	1 cut(s)	87
MroXI	GAANNNNTTC	1 cut(s)	64
MseI	TTAA	3 cut(s)	98, 197, 236
MspI	CCGG	1 cut(s)	88
Mva1269I	GAATGC	1 cut(s)	309
MwoI	GCNNNNNNNGC	2 cut(s)	76, 134
NaeI	GCCGGC	1 cut(s)	89
NarI	GGCGCC	1 cut(s)	85
NdeII	GATC	2 cut(s)	184, 354
NgoMIV	GCCGGC	1 cut(s)	87
NlaIII	CATG	1 cut(s)	415
NlaIV	GGNNCC	1 cut(s)	86
NmuCI	GTSAC	2 cut(s)	8, 53
PagI	TCATGA	1 cut(s)	411
PctI	GAATGC	1 cut(s)	309
PdiI	GCCGGC	1 cut(s)	89
PdmI	GAANNNNTTC	1 cut(s)	64
PfeI	GAWTC	1 cut(s)	408
PkrI	GCNGC	4 cut(s)	114, 117, 136, 293
PleI	GAGTC	2 cut(s)	146, 331
PluTI	GGCGCC	1 cut(s)	88
PpsI	GAGTC	2 cut(s)	146, 331
PspFI	CCCAGC	2 cut(s)	172, 287
PspN4I	GGNNCC	1 cut(s)	86
PstI	CTGCAG	1 cut(s)	120
PsuI	RGATCY	1 cut(s)	184
SaqAI	TTAA	3 cut(s)	98, 197, 236
SatI	GCNGC	4 cut(s)	113, 116, 135, 292
Sau3AI	GATC	2 cut(s)	184, 354
SchI	GAGTC	2 cut(s)	146, 332
SetI	ASST	7 cut(s)	28, 66, 81, 114, 228, 296, 351
SfaNI	GCATC	1 cut(s)	405
SfcI	CTRYAG	1 cut(s)	116
SfoI	GGCGCC	1 cut(s)	86
SsiI	CCGC	4 cut(s)	74, 128, 134, 137
SspDI	GGCGCC	1 cut(s)	84
SspMI	CTAG	1 cut(s)	92
TaaI	ACNGT	1 cut(s)	363
TaiI	ACGT	1 cut(s)	28
TauI	GCSGC	1 cut(s)	137
TfiI	GAWTC	1 cut(s)	408
Tru1I	TTAA	3 cut(s)	98, 197, 236
Tru9I	TTAA	3 cut(s)	98, 197, 236
TseFI	GTSAC	2 cut(s)	8, 53
TseI	GCWGC	3 cut(s)	112, 115, 291
Tsp45I	GTSAC	2 cut(s)	8, 53
TspGWI	ACGGA	4 cut(s)	72, 174, 180, 322
XmnI	GAANNNNTTC	1 cut(s)	64
XspI	CTAG	1 cut(s)	92

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.