RchiOBHm_Chr7g0190421

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001530
Physical Location & Seq
Reverse (-)
9498998 .. 9499668
671 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17007

Sequence Viewer

Length: 423 bp
ATGCAGAGTAACAGAGGATTCGAGCGTGTGTTCAGCTACTTTGATGAAGATGGAGACGGGAAGATCTCACCCTCGGAGCTGAGAAACAGGCTAGGGTTGATGGGAGGAGAGATGCTGCAAAACGAAGCGGAGGTTGCGGTGGGGTTTTTGGACTCCGACGGGGATGGCTTGCTGGGTTTGGATGATCTTGTTGGGTTGATGGAAGGTGGAGAAGAGGAAGAGAAAATGAAGGGTTTAAGGGAGGCCTTTGAAGTGTATGATGAGGAGGGATGCGGGTTTATAACACCTAAGAGCTTGAAGAGAATGCTTAATAGATTGGGTGAGTCCAAGTCCATTGAGGAATGCAAGGTGATGATTAATCAATTTGATTTGAATGGGGATGGCCTCATCAGCTTTGAAGAGTTCACAGTAATGATGCGATGA

Protein Analysis

140

Amino Acids

15.67

Weight (kDa)

4.29

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 7 - 30 5.1e-06 EF hand domain
EF-hand_7 PF13499 8 - 67 1.3e-08 EF-hand domain pair
EF-hand_5 PF13202 9 - 29 3.1e-06 EF hand
AIF-1 PF21008 71 - 139 7e-06 Allograft inflammatory factor 1
EF-hand_7 PF13499 78 - 139 1.2e-13 EF-hand domain pair
EF-hand_8 PF13833 94 - 139 7e-08 EF-hand domain pair
EF-hand_1 PF00036 114 - 140 8.5e-08 EF hand domain
EF-hand_5 PF13202 119 - 139 4.1e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AciI CCGC 3 cut(s) 128, 137, 273
AgsI TTSAA 4 cut(s) 251, 298, 373, 398
AluBI AGCT 4 cut(s) 36, 79, 294, 393
AluI AGCT 4 cut(s) 36, 79, 294, 393
Alw26I GTCTC 1 cut(s) 48
AoxI GGCC 2 cut(s) 243, 382
ApeKI GCWGC 1 cut(s) 115
AseI ATTAAT 1 cut(s) 357
AsuHPI GGTGA 3 cut(s) 60, 332, 361
BbvI GCAGC 1 cut(s) 102
BccI CCATC 5 cut(s) 44, 94, 158, 193, 374
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 1 cut(s) 92
BglII AGATCT 1 cut(s) 63
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
BmsI GCATC 3 cut(s) 102, 260, 405
BsaJI CCNNGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 4 cut(s) 169, 187, 275, 385
BseMII CTCAG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 120, 278
BseXI GCAGC 1 cut(s) 102
BseYI CCCAGC 1 cut(s) 172
BshFI GGCC 2 cut(s) 245, 384
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsmI GAATGC 2 cut(s) 309, 347
BsnI GGCC 2 cut(s) 245, 384
Bsp143I GATC 2 cut(s) 63, 184
BspACI CCGC 3 cut(s) 128, 137, 273
BspANI GGCC 2 cut(s) 245, 384
BspCNI CTCAG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 63, 184
Bst4CI ACNGT 1 cut(s) 409
Bst6I CTCTTC 4 cut(s) 207, 213, 293, 393
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 2 cut(s) 80, 288
BstF5I GGATG 4 cut(s) 169, 187, 275, 385
BstKTI GATC 2 cut(s) 66, 187
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 2 cut(s) 63, 184
BstMWI GCNNNNNNNGC 2 cut(s) 134, 390
BstV1I GCAGC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BsuRI GGCC 2 cut(s) 245, 384
BtsCI GGATG 4 cut(s) 169, 187, 275, 385
Cac8I GCNNGC 1 cut(s) 170
CviJI RGCY 8 cut(s) 36, 79, 91, 168, 245, 294, 384, 393
CviKI_1 RGCY 8 cut(s) 36, 79, 91, 168, 245, 294, 384, 393
DdeI CTNAG 2 cut(s) 80, 288
DpnI GATC 2 cut(s) 65, 186
DpnII GATC 2 cut(s) 63, 184
Eam1104I CTCTTC 4 cut(s) 207, 213, 293, 393
EarI CTCTTC 4 cut(s) 207, 213, 293, 393
Eco147I AGGCCT 1 cut(s) 245
Esp3I CGTCTC 1 cut(s) 48
FaiI YATR 2 cut(s) 258, 281
FauI CCCGC 1 cut(s) 266
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 4 cut(s) 176, 194, 282, 392
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 116
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 2 cut(s) 245, 384
HinfI GANTC 3 cut(s) 18, 152, 323
HphI GGTGA 3 cut(s) 60, 332, 361
Hpy166II GTNNAC 1 cut(s) 405
Hpy188I TCNGA 2 cut(s) 76, 157
Hpy8I GTNNAC 1 cut(s) 405
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 2 cut(s) 197, 223
HpyCH4III ACNGT 1 cut(s) 409
HpyCH4V TGCA 3 cut(s) 4, 118, 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 390
HpyF3I CTNAG 2 cut(s) 80, 288
Kzo9I GATC 2 cut(s) 63, 184
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 2 cut(s) 73, 158
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 3 cut(s) 102, 260, 405
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 8
MalI GATC 2 cut(s) 65, 186
MboI GATC 2 cut(s) 63, 184
MboII GAAGA 6 cut(s) 59, 73, 224, 230, 310, 410
MflI RGATCY 1 cut(s) 63
MluCI AATT 1 cut(s) 362
MlyI GAGTC 2 cut(s) 146, 332
MmeI TCCRAC 1 cut(s) 180
MseI TTAA 3 cut(s) 236, 309, 357
MslI CAYNNNNRTG 1 cut(s) 410
Mva1269I GAATGC 2 cut(s) 309, 347
MwoI GCNNNNNNNGC 2 cut(s) 134, 390
NdeII GATC 2 cut(s) 63, 184
PceI AGGCCT 1 cut(s) 245
PctI GAATGC 2 cut(s) 309, 347
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 2 cut(s) 146, 331
PpsI GAGTC 2 cut(s) 146, 331
PshBI ATTAAT 1 cut(s) 357
PsiI TTATAA 1 cut(s) 281
PspFI CCCAGC 1 cut(s) 172
PsuI RGATCY 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 3 cut(s) 236, 309, 357
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 63, 184
SchI GAGTC 2 cut(s) 146, 332
SetI ASST 8 cut(s) 38, 81, 135, 208, 289, 296, 351, 395
SfaNI GCATC 3 cut(s) 102, 260, 405
SmiMI CAYNNNNRTG 1 cut(s) 410
Sse9I AATT 1 cut(s) 362
SseBI AGGCCT 1 cut(s) 245
SsiI CCGC 3 cut(s) 128, 137, 273
SspMI CTAG 1 cut(s) 92
StuI AGGCCT 1 cut(s) 245
TaaI ACNGT 1 cut(s) 409
TaqI TCGA 1 cut(s) 21
TasI AATT 1 cut(s) 362
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 3 cut(s) 236, 309, 357
Tru9I TTAA 3 cut(s) 236, 309, 357
TseI GCWGC 1 cut(s) 115
TspDTI ATGAA 2 cut(s) 60, 242
VspI ATTAAT 1 cut(s) 357
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.