FvH4_7g03420

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001725
Physical Location & Seq
Reverse (-)
3736594 .. 3737235
642 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03420.t1

Sequence Viewer

Length: 522 bp
ATGGGTAAGGACAAGTGCCATCAACGGCAGTTCTTTACTACCGAAGATATTCTTCTTGACAGTACCTCCACCTTTCCAGAGCCAGAAGCCGAGCATAATGAAGCTAATGAAGTAGAATTTACAGAAGGGTCAGAAGAGGAGGCGTCGAGTGAAAAAGAAAATGAAAATAAGAAAACCAAAGGTGTTGAAGGCATTATTGAGATTCAAAATCCTAATTTGGTCAAGCACAAGACGGCATTGAATGCTAGAGACATTGACATTGAAAAAACAACTGAACTGTCAAGGCGTGAAAGAGAAGAGATAGAGAAACAGAGAAGCCATGATAGATACATGAAGTTGCAAGAACAGGGAAAAACTGAACAAGCAAGGAAAAATTTAGAGCGCCTAGCGCTTATACGACAACAAAGGGAGGAAGCTGCTAAAAAGAGAGCAGAAGATGGTTCTACCCCGGAAGCTGCTAAAAAGAGAGAAGAAGAAAAAGCTGCCAGAGAACAGAACCGCAAACCATTACTTAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

174

Amino Acids

20.17

Weight (kDa)

5.78

Isoelectric Point (pI)

73.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP28 PF10252 70 - 145 4.4e-26 Casein kinase substrate phosphoprotein PP28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 499
AcsI RAATTY 2 cut(s) 116, 373
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 1 cut(s) 64
AfeI AGCGCT 1 cut(s) 390
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 4 cut(s) 188, 206, 241, 263
AluBI AGCT 4 cut(s) 104, 416, 455, 482
AluI AGCT 4 cut(s) 104, 416, 455, 482
Alw26I GTCTC 1 cut(s) 243
Aor51HI AGCGCT 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 416, 455, 482
ApoI RAATTY 2 cut(s) 116, 373
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 2 cut(s) 384, 391
AsuC2I CCSGG 1 cut(s) 449
BbvI GCAGC 3 cut(s) 403, 442, 469
BccI CCATC 2 cut(s) 27, 431
BceAI ACGGC 2 cut(s) 41, 249
BcnI CCSGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 2 cut(s) 246, 386
BfoI RGCGCY 2 cut(s) 385, 392
BfrI CTTAAG 1 cut(s) 512
BisI GCNGC 3 cut(s) 417, 456, 483
BlsI GCNGC 3 cut(s) 418, 457, 484
Bme1390I CCNGG 1 cut(s) 449
BmrFI CCNGG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 449
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 447
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
BseDI CCNNGG 1 cut(s) 447
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 3 cut(s) 403, 442, 469
BsiSI CCGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 243
BsmI GAATGC 1 cut(s) 247
BspACI CCGC 1 cut(s) 499
BspTI CTTAAG 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 447
BssNI GRCGYC 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 62, 279
Bst6I CTCTTC 2 cut(s) 129, 291
BstACI GRCGYC 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 242
BstH2I RGCGCY 2 cut(s) 385, 392
BstHHI GCGC 2 cut(s) 384, 391
BstMAI GTCTC 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 242, 388
BstSCI CCNGG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 403, 442, 469
CfoI GCGC 2 cut(s) 384, 391
CseI GACGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 63
CviAII CATG 2 cut(s) 320, 331
CviJI RGCY 7 cut(s) 82, 89, 104, 318, 416, 455, 482
CviKI_1 RGCY 7 cut(s) 82, 89, 104, 318, 416, 455, 482
CviQI GTAC 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 129, 291
EarI CTCTTC 2 cut(s) 129, 291
Eco47III AGCGCT 1 cut(s) 390
FaeI CATG 2 cut(s) 323, 334
FaiI YATR 4 cut(s) 96, 321, 332, 395
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FatI CATG 2 cut(s) 319, 330
Fnu4HI GCNGC 3 cut(s) 417, 456, 483
Fsp4HI GCNGC 3 cut(s) 417, 456, 483
FspBI CTAG 2 cut(s) 246, 386
GlaI GCGC 2 cut(s) 383, 390
GluI GCNGC 3 cut(s) 417, 456, 483
HaeII RGCGCY 2 cut(s) 385, 392
HapII CCGG 1 cut(s) 449
HgaI GACGC 1 cut(s) 132
HhaI GCGC 2 cut(s) 384, 391
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 2 cut(s) 323, 334
Hin6I GCGC 2 cut(s) 382, 389
HinP1I GCGC 2 cut(s) 382, 389
HinfI GANTC 1 cut(s) 202
HpaII CCGG 1 cut(s) 449
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 2 cut(s) 56, 77
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 119, 182
HpyCH4III ACNGT 2 cut(s) 62, 279
HpyCH4V TGCA 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 388
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 2 cut(s) 323, 334
HspAI GCGC 2 cut(s) 382, 389
LpnPI CCDG 5 cut(s) 90, 96, 332, 462, 499
Lsp1109I GCAGC 3 cut(s) 403, 442, 469
MaeI CTAG 2 cut(s) 246, 386
MboII GAAGA 7 cut(s) 44, 56, 146, 308, 446, 482, 485
MluCI AATT 3 cut(s) 116, 214, 373
MnlI CCTC 4 cut(s) 76, 130, 133, 403
MroXI GAANNNNTTC 1 cut(s) 48
MseI TTAA 1 cut(s) 513
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 449
Mva1269I GAATGC 1 cut(s) 247
MwoI GCNNNNNNNGC 2 cut(s) 242, 388
NciI CCSGG 1 cut(s) 449
NlaIII CATG 2 cut(s) 323, 334
NmeAIII GCCGAG 1 cut(s) 115
PctI GAATGC 1 cut(s) 247
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 3 cut(s) 418, 457, 484
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 3 cut(s) 417, 456, 483
ScrFI CCNGG 1 cut(s) 449
SetI ASST 7 cut(s) 68, 74, 106, 184, 418, 457, 484
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 3 cut(s) 116, 214, 373
SsiI CCGC 1 cut(s) 499
SspMI CTAG 2 cut(s) 246, 386
StyD4I CCNGG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 62, 279
TaqI TCGA 1 cut(s) 146
TasI AATT 3 cut(s) 116, 214, 373
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TseI GCWGC 3 cut(s) 416, 455, 482
TspDTI ATGAA 4 cut(s) 114, 123, 177, 347
Vha464I CTTAAG 1 cut(s) 512
XapI RAATTY 2 cut(s) 116, 373
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 2 cut(s) 246, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.