28 kDa heat- and acid-stable phosphoprotein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 74 |
| AccB1I | GGYRCC | 1 cut(s) | 74 |
| AciI | CCGC | 2 cut(s) | 40, 175 |
| AclI | AACGTT | 1 cut(s) | 379 |
| AcuI | CTGAAG | 2 cut(s) | 135, 159 |
| AfaI | GTAC | 2 cut(s) | 76, 184 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 403 |
| AgeI | ACCGGT | 1 cut(s) | 32 |
| AgsI | TTSAA | 2 cut(s) | 19, 302 |
| AluBI | AGCT | 3 cut(s) | 96, 113, 269 |
| AluI | AGCT | 3 cut(s) | 96, 113, 269 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 413 |
| ApeKI | GCWGC | 2 cut(s) | 413, 440 |
| AsiGI | ACCGGT | 1 cut(s) | 32 |
| Asp718I | GGTACC | 1 cut(s) | 74 |
| AsuHPI | GGTGA | 1 cut(s) | 29 |
| BanI | GGYRCC | 1 cut(s) | 74 |
| BbvI | GCAGC | 2 cut(s) | 400, 427 |
| BccI | CCATC | 2 cut(s) | 58, 248 |
| BfaI | CTAG | 1 cut(s) | 218 |
| BisI | GCNGC | 2 cut(s) | 414, 441 |
| BlsI | GCNGC | 2 cut(s) | 415, 442 |
| BmiI | GGNNCC | 1 cut(s) | 76 |
| BsaAI | YACGTR | 1 cut(s) | 92 |
| BsaJI | CCNNGG | 1 cut(s) | 444 |
| BsaWI | WCCGGW | 1 cut(s) | 32 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 403 |
| Bse118I | RCCGGY | 1 cut(s) | 32 |
| BseDI | CCNNGG | 1 cut(s) | 444 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 403 |
| BseMII | CTCAG | 1 cut(s) | 200 |
| BseRI | GAGGAG | 3 cut(s) | 134, 308, 446 |
| BseXI | GCAGC | 2 cut(s) | 400, 427 |
| BshNI | GGYRCC | 1 cut(s) | 74 |
| BshTI | ACCGGT | 1 cut(s) | 32 |
| BsiSI | CCGG | 1 cut(s) | 33 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 403 |
| BspACI | CCGC | 2 cut(s) | 40, 175 |
| BspCNI | CTCAG | 1 cut(s) | 199 |
| BspHI | TCATGA | 1 cut(s) | 316 |
| BspLI | GGNNCC | 1 cut(s) | 76 |
| BspT107I | GGYRCC | 1 cut(s) | 74 |
| BsrFI | RCCGGY | 1 cut(s) | 32 |
| BssAI | RCCGGY | 1 cut(s) | 32 |
| BssECI | CCNNGG | 1 cut(s) | 444 |
| BssT1I | CCWWGG | 1 cut(s) | 444 |
| Bst6I | CTCTTC | 5 cut(s) | 54, 85, 111, 135, 423 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 413 |
| BstBAI | YACGTR | 1 cut(s) | 92 |
| BstC8I | GCNNGC | 1 cut(s) | 26 |
| BstDEI | CTNAG | 2 cut(s) | 186, 417 |
| BstEII | GGTNACC | 1 cut(s) | 35 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 413, 468 |
| BstPI | GGTNACC | 1 cut(s) | 35 |
| BstV1I | GCAGC | 2 cut(s) | 400, 427 |
| Cac8I | GCNNGC | 1 cut(s) | 26 |
| CaiI | CAGNNNCTG | 1 cut(s) | 413 |
| CciI | TCATGA | 1 cut(s) | 316 |
| Cfr10I | RCCGGY | 1 cut(s) | 32 |
| Csp6I | GTAC | 2 cut(s) | 75, 183 |
| CspAI | ACCGGT | 1 cut(s) | 32 |
| CviAII | CATG | 4 cut(s) | 273, 317, 328, 388 |
| CviQI | GTAC | 2 cut(s) | 75, 183 |
| DdeI | CTNAG | 2 cut(s) | 186, 417 |
| Eam1104I | CTCTTC | 5 cut(s) | 54, 85, 111, 135, 423 |
| EarI | CTCTTC | 5 cut(s) | 54, 85, 111, 135, 423 |
| Eco130I | CCWWGG | 1 cut(s) | 444 |
| Eco57I | CTGAAG | 2 cut(s) | 135, 159 |
| Eco91I | GGTNACC | 1 cut(s) | 35 |
| EcoO65I | GGTNACC | 1 cut(s) | 35 |
| EcoT14I | CCWWGG | 1 cut(s) | 444 |
| ErhI | CCWWGG | 1 cut(s) | 444 |
| FaeI | CATG | 4 cut(s) | 276, 320, 331, 391 |
| FaiI | YATR | 5 cut(s) | 197, 274, 318, 329, 389 |
| FatI | CATG | 4 cut(s) | 272, 316, 327, 387 |
| Fnu4HI | GCNGC | 2 cut(s) | 414, 441 |
| Fsp4HI | GCNGC | 2 cut(s) | 414, 441 |
| FspBI | CTAG | 1 cut(s) | 218 |
| GluI | GCNGC | 2 cut(s) | 414, 441 |
| HapII | CCGG | 1 cut(s) | 33 |
| Hin1II | CATG | 4 cut(s) | 276, 320, 331, 391 |
| HinfI | GANTC | 5 cut(s) | 134, 146, 192, 208, 391 |
| HpaII | CCGG | 1 cut(s) | 33 |
| HphI | GGTGA | 1 cut(s) | 29 |
| Hpy166II | GTNNAC | 1 cut(s) | 89 |
| Hpy188I | TCNGA | 2 cut(s) | 139, 154 |
| Hpy188III | TCNNGA | 2 cut(s) | 56, 317 |
| Hpy8I | GTNNAC | 1 cut(s) | 89 |
| HpyAV | CCTTC | 4 cut(s) | 96, 172, 232, 430 |
| HpyCH4IV | ACGT | 2 cut(s) | 91, 379 |
| HpyCH4V | TGCA | 2 cut(s) | 288, 337 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 413, 468 |
| HpyF3I | CTNAG | 2 cut(s) | 186, 417 |
| HpySE526I | ACGT | 2 cut(s) | 91, 379 |
| Hsp92II | CATG | 4 cut(s) | 276, 320, 331, 391 |
| KpnI | GGTACC | 1 cut(s) | 78 |
| LmnI | GCTCC | 1 cut(s) | 340 |
| LpnPI | CCDG | 6 cut(s) | 46, 57, 173, 274, 323, 329 |
| Lsp1109I | GCAGC | 2 cut(s) | 400, 427 |
| MaeI | CTAG | 1 cut(s) | 218 |
| MaeII | ACGT | 2 cut(s) | 91, 379 |
| MaeIII | GTNAC | 1 cut(s) | 35 |
| MluCI | AATT | 1 cut(s) | 201 |
| MlyI | GAGTC | 1 cut(s) | 143 |
| MnlI | CCTC | 9 cut(s) | 88, 112, 115, 136, 286, 324, 403, 424, 457 |
| MspI | CCGG | 1 cut(s) | 33 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 413, 468 |
| NlaIII | CATG | 4 cut(s) | 276, 320, 331, 391 |
| NlaIV | GGNNCC | 1 cut(s) | 76 |
| NmuCI | GTSAC | 1 cut(s) | 35 |
| PagI | TCATGA | 1 cut(s) | 316 |
| PfeI | GAWTC | 4 cut(s) | 146, 192, 208, 391 |
| PinAI | ACCGGT | 1 cut(s) | 32 |
| PkrI | GCNGC | 2 cut(s) | 415, 442 |
| PleI | GAGTC | 1 cut(s) | 142 |
| PpsI | GAGTC | 1 cut(s) | 142 |
| Ppu21I | YACGTR | 1 cut(s) | 92 |
| Psp1406I | AACGTT | 1 cut(s) | 379 |
| PspEI | GGTNACC | 1 cut(s) | 35 |
| PspN4I | GGNNCC | 1 cut(s) | 76 |
| PstNI | CAGNNNCTG | 1 cut(s) | 413 |
| RsaI | GTAC | 2 cut(s) | 76, 184 |
| RsaNI | GTAC | 2 cut(s) | 75, 183 |
| SatI | GCNGC | 2 cut(s) | 414, 441 |
| SchI | GAGTC | 1 cut(s) | 143 |
| SetI | ASST | 7 cut(s) | 80, 94, 98, 115, 271, 335, 382 |
| Sse9I | AATT | 1 cut(s) | 201 |
| SsiI | CCGC | 2 cut(s) | 40, 175 |
| SspMI | CTAG | 1 cut(s) | 218 |
| StyI | CCWWGG | 1 cut(s) | 444 |
| TaiI | ACGT | 2 cut(s) | 94, 382 |
| TaqI | TCGA | 1 cut(s) | 252 |
| TasI | AATT | 1 cut(s) | 201 |
| TfiI | GAWTC | 4 cut(s) | 146, 192, 208, 391 |
| TseFI | GTSAC | 1 cut(s) | 35 |
| TseI | GCWGC | 2 cut(s) | 413, 440 |
| Tsp45I | GTSAC | 1 cut(s) | 35 |
| TspDTI | ATGAA | 1 cut(s) | 261 |
| XspI | CTAG | 1 cut(s) | 218 |