28 kDa heat- and acid-stable
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 5, 120, 164 |
| AcsI | RAATTY | 1 cut(s) | 292 |
| AlwI | GGATC | 3 cut(s) | 5, 120, 164 |
| ApoI | RAATTY | 1 cut(s) | 292 |
| AsuC2I | CCSGG | 1 cut(s) | 188 |
| BccI | CCATC | 1 cut(s) | 281 |
| BcnI | CCSGG | 1 cut(s) | 188 |
| Bme1390I | CCNGG | 1 cut(s) | 188 |
| BmrFI | CCNGG | 1 cut(s) | 188 |
| BpuMI | CCSGG | 1 cut(s) | 188 |
| Bsa29I | ATCGAT | 1 cut(s) | 159 |
| BsaBI | GATNNNNATC | 1 cut(s) | 161 |
| Bse1I | ACTGG | 1 cut(s) | 32 |
| Bse8I | GATNNNNATC | 1 cut(s) | 161 |
| BseCI | ATCGAT | 1 cut(s) | 159 |
| BseGI | GGATG | 2 cut(s) | 225, 273 |
| BseJI | GATNNNNATC | 1 cut(s) | 161 |
| BseNI | ACTGG | 1 cut(s) | 32 |
| BshVI | ATCGAT | 1 cut(s) | 159 |
| BsiSI | CCGG | 1 cut(s) | 188 |
| Bsp143I | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| BspDI | ATCGAT | 1 cut(s) | 159 |
| BspHI | TCATGA | 1 cut(s) | 148 |
| BspPI | GGATC | 3 cut(s) | 5, 120, 164 |
| BsrI | ACTGG | 1 cut(s) | 32 |
| BssMI | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| Bst4CI | ACNGT | 1 cut(s) | 88 |
| BstDEI | CTNAG | 1 cut(s) | 215 |
| BstF5I | GGATG | 2 cut(s) | 225, 273 |
| BstKTI | GATC | 5 cut(s) | 13, 104, 115, 154, 159 |
| BstMBI | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| BstSCI | CCNGG | 1 cut(s) | 186 |
| BstX2I | RGATCY | 2 cut(s) | 10, 112 |
| BstYI | RGATCY | 2 cut(s) | 10, 112 |
| Bsu15I | ATCGAT | 1 cut(s) | 159 |
| BsuTUI | ATCGAT | 1 cut(s) | 159 |
| BtsCI | GGATG | 2 cut(s) | 225, 273 |
| CciI | TCATGA | 1 cut(s) | 148 |
| ClaI | ATCGAT | 1 cut(s) | 159 |
| CviAII | CATG | 1 cut(s) | 149 |
| DdeI | CTNAG | 1 cut(s) | 215 |
| DpnI | GATC | 5 cut(s) | 12, 103, 114, 153, 158 |
| DpnII | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| Eco32I | GATATC | 2 cut(s) | 163, 244 |
| EcoRV | GATATC | 2 cut(s) | 163, 244 |
| FaeI | CATG | 1 cut(s) | 152 |
| FaiI | YATR | 8 cut(s) | 110, 132, 150, 198, 204, 212, 248, 266 |
| FatI | CATG | 1 cut(s) | 148 |
| FokI | GGATG | 2 cut(s) | 232, 260 |
| HapII | CCGG | 1 cut(s) | 188 |
| Hin1II | CATG | 1 cut(s) | 152 |
| HinfI | GANTC | 1 cut(s) | 179 |
| HpaII | CCGG | 1 cut(s) | 188 |
| Hpy188I | TCNGA | 3 cut(s) | 156, 178, 184 |
| Hpy188III | TCNNGA | 1 cut(s) | 149 |
| HpyCH4III | ACNGT | 1 cut(s) | 88 |
| HpyCH4IV | ACGT | 1 cut(s) | 255 |
| HpyCH4V | TGCA | 1 cut(s) | 140 |
| HpyF3I | CTNAG | 1 cut(s) | 215 |
| HpySE526I | ACGT | 1 cut(s) | 255 |
| Hsp92II | CATG | 1 cut(s) | 152 |
| Kzo9I | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| LpnPI | CCDG | 3 cut(s) | 45, 201, 266 |
| MaeII | ACGT | 1 cut(s) | 255 |
| MalI | GATC | 5 cut(s) | 12, 103, 114, 153, 158 |
| MboI | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| MboII | GAAGA | 1 cut(s) | 300 |
| MflI | RGATCY | 2 cut(s) | 10, 112 |
| MluCI | AATT | 4 cut(s) | 144, 249, 292, 307 |
| MnlI | CCTC | 1 cut(s) | 178 |
| MseI | TTAA | 1 cut(s) | 77 |
| MslI | CAYNNNNRTG | 1 cut(s) | 201 |
| MspI | CCGG | 1 cut(s) | 188 |
| MspR9I | CCNGG | 1 cut(s) | 188 |
| NciI | CCSGG | 1 cut(s) | 188 |
| NdeII | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| NlaIII | CATG | 1 cut(s) | 152 |
| PagI | TCATGA | 1 cut(s) | 148 |
| PfeI | GAWTC | 1 cut(s) | 179 |
| PsuI | RGATCY | 2 cut(s) | 10, 112 |
| RseI | CAYNNNNRTG | 1 cut(s) | 201 |
| SaqAI | TTAA | 1 cut(s) | 77 |
| Sau3AI | GATC | 5 cut(s) | 10, 101, 112, 151, 156 |
| ScrFI | CCNGG | 1 cut(s) | 188 |
| SetI | ASST | 2 cut(s) | 258, 285 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 201 |
| Sse9I | AATT | 4 cut(s) | 144, 249, 292, 307 |
| StyD4I | CCNGG | 1 cut(s) | 186 |
| TaaI | ACNGT | 1 cut(s) | 88 |
| TaiI | ACGT | 1 cut(s) | 258 |
| TaqI | TCGA | 1 cut(s) | 159 |
| TasI | AATT | 4 cut(s) | 144, 249, 292, 307 |
| TfiI | GAWTC | 1 cut(s) | 179 |
| Tru1I | TTAA | 1 cut(s) | 77 |
| Tru9I | TTAA | 1 cut(s) | 77 |
| TspDTI | ATGAA | 3 cut(s) | 137, 219, 239 |
| XapI | RAATTY | 1 cut(s) | 292 |