Rh5BG071300

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001725
Physical Location & Seq
Forward (+)
5819563 .. 5822160
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG071300.1

Sequence Viewer

Length: 585 bp
ATGGGCAGAGGAAAGTACAAGAACAAGCCCACTGGTCAGCGCCAGTTCTCCAGTCGCGAAGATCTTCTTGCCGGTACCTCCACCCGTCCCCGTTCTTTTTCAAAGCGAGAAGCTGAACATGAGGAAGAACCGGAAGAGGATTTTGTATCCTTCGAAGACGGGTCTGATGAAGAATCTGATGGACAAGATGAAAAGAAGAAAGGCGTTCAAGGTATAATCGAGATTGAAAACCCTAATTTGGTAAAGCCAAAGACCTTGAAAGCTAAAGACATCGATATTGGAAAACCAACTGAACTCTCACGGCGTGAAAGGGAAGAACTAGAGAAGCAAAGAGCCCGTGAGAGATATATGAAGTTGCAAGAACAGGGGAAAACTGAACAAGCAAGGAAAGATTTAGGGAGGAAGCTGCTAAAAAGCGAGAAGAAGAAAAAAATGCTAAAGAACAGAAGAAGGCTGAATCCCGCAAATGATCATCTCAAAAGTGACCTGCAAAACAAGTTTGTGCTGCTGTTACAAGAACCATGTGCTCTATGCTGCTTTATAACTAAAATTACACTCCCATCATCCCATTTGGTGATCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

194

Amino Acids

22.51

Weight (kDa)

9.23

Isoelectric Point (pI)

64.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP28 PF10252 77 - 150 7.1e-19 Casein kinase substrate phosphoprotein PP28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc36I ACCTGC 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 74
AccII CGCG 1 cut(s) 57
AciI CCGC 1 cut(s) 462
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 2 cut(s) 17, 76
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 4 cut(s) 102, 209, 227, 259
AluBI AGCT 3 cut(s) 113, 263, 406
AluI AGCT 3 cut(s) 113, 263, 406
Alw21I GWGCWC 1 cut(s) 529
ApeKI GCWGC 3 cut(s) 406, 505, 534
Asp700I GAANNNNTTC 1 cut(s) 63
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 42
AsuII TTCGAA 1 cut(s) 153
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 3 cut(s) 393, 492, 521
BccI CCATC 2 cut(s) 173, 568
BceAI ACGGC 1 cut(s) 317
BciVI GTATCC 1 cut(s) 157
BclI TGATCA 1 cut(s) 469
BfaI CTAG 1 cut(s) 320
BfoI RGCGCY 1 cut(s) 43
BfuAI ACCTGC 1 cut(s) 495
BfuI GTATCC 1 cut(s) 157
BglII AGATCT 1 cut(s) 61
BisI GCNGC 3 cut(s) 407, 506, 535
BlsI GCNGC 3 cut(s) 408, 507, 536
BmiI GGNNCC 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 34
Bpu14I TTCGAA 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 130
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 37, 43, 51
BseCI ATCGAT 1 cut(s) 273
BseGI GGATG 1 cut(s) 563
BseLI CCNNNNNNNGG 1 cut(s) 238
BseNI ACTGG 3 cut(s) 37, 43, 51
BseXI GCAGC 3 cut(s) 393, 492, 521
Bsh1236I CGCG 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 74
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 529
BsiSI CCGG 2 cut(s) 72, 131
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 337, 529
Bsp143I GATC 3 cut(s) 61, 469, 576
Bsp68I TCGCGA 1 cut(s) 57
BspACI CCGC 1 cut(s) 462
BspDI ATCGAT 1 cut(s) 273
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 495
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 3 cut(s) 37, 43, 51
BssAI RCCGGY 1 cut(s) 71
BssMI GATC 3 cut(s) 61, 469, 576
Bst6I CTCTTC 1 cut(s) 129
BstBI TTCGAA 1 cut(s) 153
BstF5I GGATG 1 cut(s) 563
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 3 cut(s) 64, 472, 579
BstMBI GATC 3 cut(s) 61, 469, 576
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 3 cut(s) 393, 492, 521
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 273
BsuI GTATCC 1 cut(s) 157
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 1 cut(s) 563
BtsIMutI CAGTG 1 cut(s) 30
BtuMI TCGCGA 1 cut(s) 57
BveI ACCTGC 1 cut(s) 495
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 71
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 16, 75
CviAII CATG 2 cut(s) 119, 522
CviJI RGCY 7 cut(s) 28, 113, 247, 263, 335, 406, 454
CviKI_1 RGCY 7 cut(s) 28, 113, 247, 263, 335, 406, 454
CviQI GTAC 2 cut(s) 16, 75
DpnI GATC 3 cut(s) 63, 471, 578
DpnII GATC 3 cut(s) 61, 469, 576
DraIII CACNNNGTG 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 337
FaeI CATG 2 cut(s) 122, 525
FaiI YATR 7 cut(s) 120, 215, 348, 350, 523, 532, 542
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 72
FatI CATG 2 cut(s) 118, 521
FauI CCCGC 1 cut(s) 469
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 3 cut(s) 407, 506, 535
FokI GGATG 1 cut(s) 550
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 3 cut(s) 407, 506, 535
FspBI CTAG 1 cut(s) 320
GlaI GCGC 1 cut(s) 41
GluI GCNGC 3 cut(s) 407, 506, 535
GsuI CTGGAG 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 43
HapII CCGG 2 cut(s) 72, 131
HhaI GCGC 1 cut(s) 42
Hin1II CATG 2 cut(s) 122, 525
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 2 cut(s) 173, 457
HpaII CCGG 2 cut(s) 72, 131
Hpy188I TCNGA 2 cut(s) 166, 178
Hpy188III TCNNGA 2 cut(s) 56, 220
HpyAV CCTTC 2 cut(s) 160, 444
HpyCH4V TGCA 2 cut(s) 358, 490
Hsp92II CATG 2 cut(s) 122, 525
HspAI GCGC 1 cut(s) 40
KpnI GGTACC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 61, 469, 576
LpnPI CCDG 7 cut(s) 18, 56, 64, 85, 144, 350, 500
Lsp1109I GCAGC 3 cut(s) 393, 492, 521
MaeI CTAG 1 cut(s) 320
MaeIII GTNAC 2 cut(s) 482, 510
MalI GATC 3 cut(s) 63, 471, 578
MboI GATC 3 cut(s) 61, 469, 576
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 337, 529
MluCI AATT 2 cut(s) 235, 549
MnlI CCTC 4 cut(s) 88, 115, 130, 393
MroXI GAANNNNTTC 1 cut(s) 63
MspI CCGG 2 cut(s) 72, 131
MvnI CGCG 1 cut(s) 57
NdeII GATC 3 cut(s) 61, 469, 576
NlaIII CATG 2 cut(s) 122, 525
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 482
NruI TCGCGA 1 cut(s) 57
NspV TTCGAA 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 173, 457
PkrI GCNGC 3 cut(s) 408, 507, 536
PsiI TTATAA 1 cut(s) 542
PspN4I GGNNCC 1 cut(s) 76
PsuI RGATCY 1 cut(s) 61
RruI TCGCGA 1 cut(s) 57
RsaI GTAC 2 cut(s) 17, 76
RsaNI GTAC 2 cut(s) 16, 75
SatI GCNGC 3 cut(s) 407, 506, 535
Sau3AI GATC 3 cut(s) 61, 469, 576
SduI GDGCHC 2 cut(s) 337, 529
SetI ASST 7 cut(s) 80, 115, 214, 257, 265, 408, 489
SfuI TTCGAA 1 cut(s) 153
Sse9I AATT 2 cut(s) 235, 549
SsiI CCGC 1 cut(s) 462
SspMI CTAG 1 cut(s) 320
TaqI TCGA 4 cut(s) 153, 219, 273, 579
TasI AATT 2 cut(s) 235, 549
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 2 cut(s) 173, 457
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 3 cut(s) 406, 505, 534
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 3 cut(s) 183, 204, 365
TspRI CASTG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.