RchiOBHm_Chr5g0009661

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001725
Physical Location & Seq
Forward (+)
6397405 .. 6398867
1463 bp
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UTR
Exon/CDS
Intron
PRQ29052

Sequence Viewer

Length: 453 bp
ATGGATAGGAGTGAAGCTGAACATGAGGAAGAACCGGAAGAGGATTTTGTATCCTTCGAAGATGGGTCTGATGAAGAATCTGATGGACAGGATGAAAAGAAGAAAGGCGTTCAAGGTATAATCGAGATTGAAAATCCTAACGTGGTAAAGCCGAAGACCTTGAAAGCTAAAGACATCGATATTGGAAAACCTACTGAACTCTCACGGCGTGAAAGGGAAGAACTAGAGAAGCAAAGAGCCCGTGAGAGATATATGAAGTTGCAAGAACAGGGGAAAACTGAACAAGCAAGGAAAGATTTAGAGCGTTTGGCGCTTATACGACTACAGAGGGAGGAAGCTGCTAAAAAGCGAGAAGAAGAAAAAAATGGTAATGTTCCAATATCACCTCTACCAGCTTATTTCGTTCCTCCTTTTCTTGGGTCCCTTTCTCTTCTGGATTTTCAAAATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

150

Amino Acids

17.35

Weight (kDa)

4.85

Isoelectric Point (pI)

70.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP28 PF10252 45 - 122 4.3e-30 Casein kinase substrate phosphoprotein PP28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 445
AdeI CACNNNGTG 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 416
AgsI TTSAA 4 cut(s) 113, 131, 163, 443
AluBI AGCT 4 cut(s) 17, 167, 338, 395
AluI AGCT 4 cut(s) 17, 167, 338, 395
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 445
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 1 cut(s) 420
AsuHPI GGTGA 1 cut(s) 375
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 420
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 325
BccI CCATC 2 cut(s) 56, 77
BceAI ACGGC 1 cut(s) 221
BciVI GTATCC 1 cut(s) 61
BfaI CTAG 1 cut(s) 224
BfmI CTRYAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 314
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 2 cut(s) 421, 422
BpiI GAAGAC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 57
Bsa29I ATCGAT 1 cut(s) 177
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 416
BseCI ATCGAT 1 cut(s) 177
BseGI GGATG 1 cut(s) 97
BseLI CCNNNNNNNGG 1 cut(s) 416
BseXI GCAGC 1 cut(s) 325
BshVI ATCGAT 1 cut(s) 177
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 406
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 421, 422
BspT104I TTCGAA 1 cut(s) 57
Bst6I CTCTTC 2 cut(s) 33, 435
BstBI TTCGAA 1 cut(s) 57
BstF5I GGATG 1 cut(s) 97
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 1 cut(s) 313
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 161
Bsu15I ATCGAT 1 cut(s) 177
BsuI GTATCC 1 cut(s) 61
BsuTUI ATCGAT 1 cut(s) 177
BtsCI GGATG 1 cut(s) 97
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 420
ClaI ATCGAT 1 cut(s) 177
CviAII CATG 1 cut(s) 23
CviJI RGCY 6 cut(s) 17, 151, 167, 239, 338, 395
CviKI_1 RGCY 6 cut(s) 17, 151, 167, 239, 338, 395
DraIII CACNNNGTG 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 33, 435
EarI CTCTTC 2 cut(s) 33, 435
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 1 cut(s) 26
FaiI YATR 5 cut(s) 24, 119, 252, 254, 317
FaqI GGGAC 1 cut(s) 406
FatI CATG 1 cut(s) 22
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 1 cut(s) 224
GlaI GCGC 1 cut(s) 312
GluI GCNGC 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 314
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 313
Hin1II CATG 1 cut(s) 26
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HinfI GANTC 1 cut(s) 77
HpaII CCGG 1 cut(s) 35
HphI GGTGA 1 cut(s) 375
Hpy188I TCNGA 2 cut(s) 70, 82
Hpy188III TCNNGA 2 cut(s) 124, 434
HpyAV CCTTC 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 26
HspAI GCGC 1 cut(s) 311
KflI GGGWCCC 1 cut(s) 420
LpnPI CCDG 5 cut(s) 48, 74, 254, 405, 419
Lsp1109I GCAGC 1 cut(s) 325
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 1 cut(s) 445
MnlI CCTC 6 cut(s) 19, 34, 321, 325, 396, 417
MseI TTAA 1 cut(s) 451
MspI CCGG 1 cut(s) 35
MwoI GCNNNNNNNGC 1 cut(s) 310
NlaIII CATG 1 cut(s) 26
NlaIV GGNNCC 2 cut(s) 421, 422
NspV TTCGAA 1 cut(s) 57
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 340
PpuMI RGGWCCY 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 421, 422
PspPI GGNCC 1 cut(s) 420
PspPPI RGGWCCY 1 cut(s) 420
SaqAI TTAA 1 cut(s) 451
SatI GCNGC 1 cut(s) 339
Sau96I GGNCC 1 cut(s) 420
SduI GDGCHC 1 cut(s) 241
SetI ASST 9 cut(s) 19, 118, 144, 161, 169, 193, 340, 388, 397
SfcI CTRYAG 1 cut(s) 323
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 1 cut(s) 420
Sse9I AATT 1 cut(s) 445
SspMI CTAG 1 cut(s) 224
TaiI ACGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 57, 123, 177
TasI AATT 1 cut(s) 445
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 451
Tru9I TTAA 1 cut(s) 451
TseI GCWGC 1 cut(s) 338
TspDTI ATGAA 3 cut(s) 87, 108, 269
VpaK11BI GGWCC 1 cut(s) 420
XapI RAATTY 1 cut(s) 445
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.