Rmu_sc0006311.1_g000003

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006311.1
OG0001725
Physical Location & Seq
Reverse (-)
8863 .. 11730
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006311.1_g000003.1.cds

Sequence Viewer

Length: 399 bp
atgggcagaggaaagtacaagaacaagcccactggtcagcgccagttctccagtcgcgaagatcttcaagctgaacatgaggaagaaccggaagaggattttgtatccttcgaagatgggtctgatgaagaatctgatggacaagatgaaaagaagaaaggcgttcaaggtataatcgagattgaaaatcctaatttggtaaagccgaagaccttgaaagctaaagacatcgatagcgtttggcgcttatacgacaacagagggaggaagctgctaaaaagcgagaagaagaaaaaaatggccaagaggtgtcatagcatcagccaagatgttcgctttacagtggatatgcttgaaagaaatgttgttgaagttccttgtaaagttgcaatagcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

132

Amino Acids

15.22

Weight (kDa)

6.76

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 300
AfaI GTAC 1 cut(s) 17
AgsI TTSAA 6 cut(s) 68, 167, 185, 217, 356, 371
AluBI AGCT 4 cut(s) 71, 221, 271, 395
AluI AGCT 4 cut(s) 71, 221, 271, 395
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 271
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 2 cut(s) 42, 246
AsuII TTCGAA 1 cut(s) 111
BalI TGGCCA 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 258
BccI CCATC 2 cut(s) 110, 131
BciVI GTATCC 1 cut(s) 115
BfoI RGCGCY 2 cut(s) 43, 247
BfuI GTATCC 1 cut(s) 115
BglII AGATCT 1 cut(s) 61
BisI GCNGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 273
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 34
Bpu14I TTCGAA 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 88
Bse1I ACTGG 3 cut(s) 37, 43, 51
BseCI ATCGAT 1 cut(s) 231
BseNI ACTGG 3 cut(s) 37, 43, 51
BseXI GCAGC 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 302
BshVI ATCGAT 1 cut(s) 231
BsiSI CCGG 1 cut(s) 89
BsnI GGCC 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 111
Bsp143I GATC 1 cut(s) 61
Bsp68I TCGCGA 1 cut(s) 57
BspANI GGCC 1 cut(s) 302
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 1 cut(s) 57
BspT104I TTCGAA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 37, 43, 51
BssMI GATC 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 1 cut(s) 87
BstBI TTCGAA 1 cut(s) 111
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 2 cut(s) 43, 247
BstHHI GCGC 2 cut(s) 42, 246
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 1 cut(s) 243
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 1 cut(s) 302
BsuTUI ATCGAT 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 30, 348
BtuMI TCGCGA 1 cut(s) 57
CfoI GCGC 2 cut(s) 42, 246
ClaI ATCGAT 1 cut(s) 231
Csp6I GTAC 1 cut(s) 16
CviAII CATG 1 cut(s) 77
CviJI RGCY 8 cut(s) 28, 71, 205, 221, 271, 302, 324, 395
CviKI_1 RGCY 8 cut(s) 28, 71, 205, 221, 271, 302, 324, 395
CviQI GTAC 1 cut(s) 16
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
FaeI CATG 1 cut(s) 80
FaiI YATR 5 cut(s) 78, 173, 250, 315, 350
FatI CATG 1 cut(s) 76
Fnu4HI GCNGC 1 cut(s) 272
Fsp4HI GCNGC 1 cut(s) 272
GlaI GCGC 2 cut(s) 41, 245
GluI GCNGC 1 cut(s) 272
GsuI CTGGAG 1 cut(s) 34
HaeII RGCGCY 2 cut(s) 43, 247
HaeIII GGCC 1 cut(s) 302
HapII CCGG 1 cut(s) 89
HhaI GCGC 2 cut(s) 42, 246
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 2 cut(s) 40, 244
HinP1I GCGC 2 cut(s) 40, 244
HinfI GANTC 1 cut(s) 131
HpaII CCGG 1 cut(s) 89
Hpy188I TCNGA 2 cut(s) 124, 136
Hpy188III TCNNGA 2 cut(s) 56, 178
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4V TGCA 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 243
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 2 cut(s) 40, 244
Kzo9I GATC 1 cut(s) 61
LpnPI CCDG 4 cut(s) 18, 56, 64, 102
Lsp1109I GCAGC 1 cut(s) 258
LweI GCATC 1 cut(s) 327
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MflI RGATCY 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 1 cut(s) 193
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 5 cut(s) 73, 88, 254, 258, 300
Mox20I TGGCCA 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 302
MspI CCGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 243
NdeII GATC 1 cut(s) 61
NlaIII CATG 1 cut(s) 80
NruI TCGCGA 1 cut(s) 57
NspV TTCGAA 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 1 cut(s) 273
PsuI RGATCY 1 cut(s) 61
RruI TCGCGA 1 cut(s) 57
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SatI GCNGC 1 cut(s) 272
Sau3AI GATC 1 cut(s) 61
SetI ASST 7 cut(s) 73, 172, 215, 223, 273, 311, 397
SfaNI GCATC 1 cut(s) 327
SfuI TTCGAA 1 cut(s) 111
Sse9I AATT 1 cut(s) 193
TaaI ACNGT 1 cut(s) 343
TaqI TCGA 3 cut(s) 111, 177, 231
TasI AATT 1 cut(s) 193
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 2 cut(s) 37, 348
TseI GCWGC 1 cut(s) 271
TspDTI ATGAA 2 cut(s) 141, 162
TspRI CASTG 2 cut(s) 37, 348
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.