Rorug04G0444600

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001725
Physical Location & Seq
Reverse (-)
60075897 .. 60079514
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0444600.1

Sequence Viewer

Length: 1776 bp
ATGAAGAACCTCTTCACCATTCTCGTCCTCGCAGTTCTAATCACCTGCGGTGCGACCTTCGTCAGATCGGACGGCTCCGATCACCGCTACAATGAAGGCGACGTCGTCCCCCTCTACGCCAATAAGGTCGGCCCCTTCCACAACCCCAGTGAGACGTATCGCTATTTTGATCTGCCATTTTGCTCACCAGGTGATGTGAAGGAGAAGAGGGAAGCTCTCGGGGAGGTGCTGAATGGAGATCGTCTAGTGAGTGCTCCTTATAAACTTGAGTTCAGGAAAGAGAAAGACACCGAAGTTGCTTGTAGAAGGAAGCTGACCAAGGAGGAAGTTGCGCAGTTTCGAAAGGCTGTGAAAAAGGATTACTACTTTCAAATGTACTATGACGACTTGCCCATCTGGGGGTTCTTAGGAAAGGTTGACAAGGAAGGAAAAACTGATCCAAGTGAATACAAATATTTCCTGTACAAGCACATCCAATTTGAAATTCTCTACAATAAAGATCGTGTGATCGAAATTGGCGCCAAGATGGATCCTCATTCAGTTGTGGACTTGACAGAGGACAAGGACGTTGATGCGGATTTCATGTACACTGTGAAATGGAGAGAGACAAATATTCCATTTGAGAATAGAATGGATAAGTACTCACAGTCCTCTTCACTGCCACATCACTTGGAAATTCATTGGTTCTCAATCATAAATTCCTGTGTCACAGTTCTCCTTTTGACGGGTTTTCTTGCAACAATTCTCATGCGAGTCTTGAAGAATGATTTCATGAAGTATGCCCAAGATGAGGAAGCAGCCGATGACCAAGAAGAGACAGGATGGAAGTACATTCATGGTGATGTTTTCCGGTTTCCCAAGTACAAATCGTTGTTTGCTGCTGCCCATGGTTCTGGAACCCAGTTGTTCACCCTCACAGTGTTCATTTTTATGCTTGCACTGGTCGGTGTATTTTATCCATACAACCGAGGAGCTCTGTTCACTGCTCTTGTGGTCATATACGCACTCACATCTGGCATTGCGGGATATACTTCAACCTCTTTCTACTGTCAGCTTGAAGGGACAAATTGGGTTAGAAATTTATTACTCACTGGGTGCCTTTTTTGTGGGCCTCTGTTCCTGACATTCTGCTTCCTTAACACAGTTGCAATTGCTTATAGTGCAACTGCAGCTCTTCCTTTTGGTACTATTGTGGTAATTGTCCTTATCTGGACATTGGTAACAGCACCATTGCTTGTCTTGGGTGGTATTGCTGGGAAGAACAGTAAGGTTGAGTTCCATGCTCCTTGTCGCACCACAAAGTATCCTCGAGAAATTCCAACTCTGCCATGGTACAGGACAACTGTTCCTCAGATGGCAATGGCAGGATTTCTCCCTTTCAGTGCGATTTACATTGAGCTTTACTACATATTTGCCAGTGTCTGGGGTCACAGGATTTACACCATCTACAGCATTCTGTTTATTGTTTTTCTTATTCTTTTGATTGTCACTGCCTTTATAACTGTGGCTTTGACCTACTTCCAACTTGCTGCTGAAGATCATGAGTGGTGGTGGAGGTCTTTTCTTTGCGGTGGATCGACTGGCCTATTTATCTATGGTTACTGCTTGTATTACTACTATGCTAGATCAGATATGTCTGGTTTTATGCAGACATCGTTCTTCTTTGGTTACATGGCTTGCATTTGCTATGGCTTCTTTCTCATGCTTGGCACTGTTGGATTTCGTGCTGCTTTGCTCTTTGTTCGTCACATTTACAAGTCTATCAAGTGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

591

Amino Acids

67.92

Weight (kDa)

7.16

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 52 - 548 1.4e-182 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 261, 1499
AarI CACCTGC 1 cut(s) 53
AatII GACGTC 1 cut(s) 105
Acc16I TGCGCA 1 cut(s) 333
Acc36I ACCTGC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 518, 1095
AccB7I CCANNNNNTGG 1 cut(s) 1422
AciI CCGC 5 cut(s) 48, 85, 575, 1022, 1569
AclWI GGATC 4 cut(s) 431, 524, 537, 1582
AcsI RAATTY 5 cut(s) 483, 675, 697, 1078, 1314
AcuI CTGAAG 1 cut(s) 1554
AcyI GRCGYC 2 cut(s) 102, 519
AdeI CACNNNGTG 2 cut(s) 191, 1095
AfaI GTAC 8 cut(s) 377, 464, 587, 641, 830, 863, 1186, 1334
AfiI CCNNNNNNNGG 5 cut(s) 398, 399, 724, 790, 1422
AgsI TTSAA 5 cut(s) 371, 482, 760, 1035, 1058
AjnI CCWGG 1 cut(s) 187
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AluBI AGCT 6 cut(s) 215, 313, 974, 1054, 1172, 1399
AluI AGCT 6 cut(s) 215, 313, 974, 1054, 1172, 1399
Alw21I GWGCWC 2 cut(s) 256, 976
Alw26I GTCTC 3 cut(s) 146, 599, 809
AlwI GGATC 4 cut(s) 431, 524, 537, 1582
AlwNI CAGNNNCTG 1 cut(s) 1422
Ama87I CYCGRG 2 cut(s) 218, 1308
AoxI GGCC 3 cut(s) 130, 1109, 1582
ApeKI GCWGC 6 cut(s) 797, 878, 881, 1169, 1529, 1727
ApoI RAATTY 5 cut(s) 483, 675, 697, 1078, 1314
Asp700I GAANNNNTTC 2 cut(s) 11, 767
AspLEI GCGC 2 cut(s) 334, 521
AspS9I GGNCC 2 cut(s) 131, 1109
AsuHPI GGTGA 7 cut(s) 7, 34, 74, 177, 203, 851, 901
AsuII TTCGAA 1 cut(s) 340
AvaI CYCGRG 2 cut(s) 218, 1308
BaeI ACNNNNGTAYC 2 cut(s) 1176, 1209
BamHI GGATCC 1 cut(s) 529
BanI GGYRCC 2 cut(s) 518, 1095
BanII GRGCYC 1 cut(s) 976
Bbv12I GWGCWC 2 cut(s) 256, 976
BbvI GCAGC 6 cut(s) 809, 865, 868, 1181, 1516, 1714
BccI CCATC 5 cut(s) 401, 520, 816, 1348, 1451
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 1314
BcoDI GTCTC 3 cut(s) 146, 599, 809
BfaI CTAG 2 cut(s) 245, 1623
BfmI CTRYAG 2 cut(s) 1167, 1447
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 1 cut(s) 53
BfuI GTATCC 1 cut(s) 1314
BisI GCNGC 6 cut(s) 798, 879, 882, 1170, 1530, 1728
BlsI GCNGC 6 cut(s) 799, 880, 883, 1171, 1531, 1729
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 1 cut(s) 189
BmeT110I CYCGRG 2 cut(s) 218, 1308
BmgT120I GGNCC 2 cut(s) 131, 1109
BmiI GGNNCC 6 cut(s) 76, 133, 520, 531, 898, 1097
BmrFI CCNGG 1 cut(s) 189
BmrI ACTGGG 3 cut(s) 141, 895, 1101
BmsI GCATC 1 cut(s) 562
BmuI ACTGGG 3 cut(s) 141, 895, 1101
BoxI GACNNNNGTC 1 cut(s) 59
BplI GAGNNNNNCTC 2 cut(s) 199, 231
Bpu14I TTCGAA 1 cut(s) 340
BpuEI CTTGAG 1 cut(s) 287
BsaHI GRCGYC 2 cut(s) 102, 519
BsaJI CCNNGG 4 cut(s) 318, 886, 967, 1328
BsaWI WCCGGW 1 cut(s) 849
Bsc4I CCNNNNNNNGG 5 cut(s) 398, 399, 724, 790, 1422
Bse1I ACTGG 6 cut(s) 147, 901, 945, 1096, 1416, 1585
Bse3DI GCAATG 3 cut(s) 1017, 1229, 1365
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 4 cut(s) 318, 886, 967, 1328
BseGI GGATG 2 cut(s) 471, 827
BseLI CCNNNNNNNGG 5 cut(s) 398, 399, 724, 790, 1422
BseMI GCAATG 3 cut(s) 1017, 1229, 1365
BseMII CTCAG 1 cut(s) 1364
BseNI ACTGG 6 cut(s) 147, 901, 945, 1096, 1416, 1585
BseRI GAGGAG 1 cut(s) 984
BseXI GCAGC 6 cut(s) 809, 865, 868, 1181, 1516, 1714
BseYI CCCAGC 1 cut(s) 1253
BshFI GGCC 3 cut(s) 132, 1111, 1584
BshNI GGYRCC 2 cut(s) 518, 1095
BsiHKAI GWGCWC 2 cut(s) 256, 976
BsiHKCI CYCGRG 2 cut(s) 218, 1308
BsiSI CCGG 1 cut(s) 850
BslFI GGGAC 2 cut(s) 92, 1075
BslI CCNNNNNNNGG 5 cut(s) 398, 399, 724, 790, 1422
BsmAI GTCTC 3 cut(s) 146, 599, 809
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 92, 1075
BsmI GAATGC 1 cut(s) 1452
BsnI GGCC 3 cut(s) 132, 1111, 1584
BsoBI CYCGRG 2 cut(s) 218, 1308
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 2 cut(s) 256, 976
Bsp1407I TGTACA 2 cut(s) 462, 585
Bsp19I CCATGG 2 cut(s) 886, 1328
BspACI CCGC 5 cut(s) 48, 85, 575, 1022, 1569
BspANI GGCC 3 cut(s) 132, 1111, 1584
BspCNI CTCAG 1 cut(s) 1363
BspHI TCATGA 2 cut(s) 771, 1540
BspLI GGNNCC 6 cut(s) 76, 133, 520, 531, 898, 1097
BspMAI CTGCAG 1 cut(s) 1171
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 4 cut(s) 431, 524, 537, 1582
BspQI GCTCTTC 1 cut(s) 1179
BspT104I TTCGAA 1 cut(s) 340
BspT107I GGYRCC 2 cut(s) 518, 1095
BsrDI GCAATG 3 cut(s) 1017, 1229, 1365
BsrGI TGTACA 2 cut(s) 462, 585
BsrI ACTGG 6 cut(s) 147, 901, 945, 1096, 1416, 1585
BssECI CCNNGG 4 cut(s) 318, 886, 967, 1328
BssNI GRCGYC 2 cut(s) 102, 519
BssT1I CCWWGG 3 cut(s) 318, 886, 1328
Bst2UI CCWGG 1 cut(s) 189
Bst6I CTCTTC 5 cut(s) 17, 200, 658, 807, 1179
BstACI GRCGYC 2 cut(s) 102, 519
BstAUI TGTACA 2 cut(s) 462, 585
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 2 cut(s) 936, 1678
BstDEI CTNAG 2 cut(s) 406, 1350
BstDSI CCRYGG 2 cut(s) 886, 1328
BstF5I GGATG 2 cut(s) 471, 827
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 2 cut(s) 334, 521
BstMAI GTCTC 3 cut(s) 146, 599, 809
BstMWI GCNNNNNNNGC 2 cut(s) 1160, 1169
BstNI CCWGG 1 cut(s) 189
BstPAI GACNNNNGTC 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 2 cut(s) 1167, 1447
BstV1I GCAGC 6 cut(s) 809, 865, 868, 1181, 1516, 1714
BstX2I RGATCY 1 cut(s) 529
BstXI CCANNNNNNTGG 1 cut(s) 893
BstYI RGATCY 1 cut(s) 529
BsuI GTATCC 1 cut(s) 1314
BsuRI GGCC 3 cut(s) 132, 1111, 1584
BtgI CCRYGG 2 cut(s) 886, 1328
BtsCI GGATG 2 cut(s) 471, 827
BtsI GCAGTG 3 cut(s) 656, 981, 1488
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 2 cut(s) 936, 1678
CaiI CAGNNNCTG 1 cut(s) 1422
CciI TCATGA 2 cut(s) 771, 1540
CfoI GCGC 2 cut(s) 334, 521
Cfr13I GGNCC 2 cut(s) 131, 1109
CsiI ACCWGGT 1 cut(s) 187
Csp6I GTAC 8 cut(s) 376, 463, 586, 640, 829, 862, 1185, 1333
CspCI CAANNNNNGTGG 2 cut(s) 651, 686
CviQI GTAC 8 cut(s) 376, 463, 586, 640, 829, 862, 1185, 1333
DdeI CTNAG 2 cut(s) 406, 1350
DinI GGCGCC 1 cut(s) 520
DraIII CACNNNGTG 2 cut(s) 191, 1095
Eam1104I CTCTTC 5 cut(s) 17, 200, 658, 807, 1179
EarI CTCTTC 5 cut(s) 17, 200, 658, 807, 1179
Ecl136II GAGCTC 1 cut(s) 974
Eco130I CCWWGG 3 cut(s) 318, 886, 1328
Eco24I GRGCYC 1 cut(s) 976
Eco53kI GAGCTC 1 cut(s) 974
Eco57I CTGAAG 1 cut(s) 1554
Eco88I CYCGRG 2 cut(s) 218, 1308
EcoICRI GAGCTC 1 cut(s) 974
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 3 cut(s) 318, 886, 1328
EcoT38I GRGCYC 1 cut(s) 976
EgeI GGCGCC 1 cut(s) 520
EheI GGCGCC 1 cut(s) 520
ErhI CCWWGG 3 cut(s) 318, 886, 1328
Esp3I CGTCTC 1 cut(s) 146
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 2 cut(s) 92, 1075
FauI CCCGC 1 cut(s) 1015
Fnu4HI GCNGC 6 cut(s) 798, 879, 882, 1170, 1530, 1728
FokI GGATG 2 cut(s) 458, 834
FriOI GRGCYC 1 cut(s) 976
Fsp4HI GCNGC 6 cut(s) 798, 879, 882, 1170, 1530, 1728
FspBI CTAG 2 cut(s) 245, 1623
FspI TGCGCA 1 cut(s) 333
GlaI GCGC 2 cut(s) 333, 520
GluI GCNGC 6 cut(s) 798, 879, 882, 1170, 1530, 1728
GsaI CCCAGC 1 cut(s) 1257
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 3 cut(s) 132, 1111, 1584
HapII CCGG 1 cut(s) 850
HhaI GCGC 2 cut(s) 334, 521
Hin1I GRCGYC 2 cut(s) 102, 519
Hin6I GCGC 2 cut(s) 332, 519
HinP1I GCGC 2 cut(s) 332, 519
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 1 cut(s) 753
HpaII CCGG 1 cut(s) 850
HphI GGTGA 7 cut(s) 7, 34, 74, 177, 203, 851, 901
Hpy166II GTNNAC 5 cut(s) 418, 547, 588, 909, 981
Hpy188I TCNGA 5 cut(s) 65, 70, 79, 1353, 1630
Hpy188III TCNNGA 8 cut(s) 274, 757, 772, 894, 1120, 1210, 1310, 1541
Hpy8I GTNNAC 5 cut(s) 418, 547, 588, 909, 981
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 7 cut(s) 67, 89, 145, 193, 300, 419, 1052
HpyCH4IV ACGT 3 cut(s) 102, 155, 567
HpyCH4V TGCA 7 cut(s) 737, 938, 1148, 1163, 1169, 1648, 1680
HpyF10VI GCNNNNNNNGC 2 cut(s) 1160, 1169
HpyF3I CTNAG 2 cut(s) 406, 1350
HpySE526I ACGT 3 cut(s) 102, 155, 567
Hsp92I GRCGYC 2 cut(s) 102, 519
HspAI GCGC 2 cut(s) 332, 519
KasI GGCGCC 1 cut(s) 518
LguI GCTCTTC 1 cut(s) 1179
LmnI GCTCC 4 cut(s) 80, 259, 971, 1288
Lsp1109I GCAGC 6 cut(s) 809, 865, 868, 1181, 1516, 1714
LweI GCATC 1 cut(s) 562
MabI ACCWGGT 1 cut(s) 187
MaeI CTAG 2 cut(s) 245, 1623
MaeII ACGT 3 cut(s) 102, 155, 567
MaeIII GTNAC 7 cut(s) 706, 1219, 1427, 1486, 1598, 1667, 1745
MfeI CAATTG 1 cut(s) 1149
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 2 cut(s) 256, 976
Mly113I GGCGCC 1 cut(s) 519
MlyI GAGTC 1 cut(s) 762
MmeI TCCRAC 3 cut(s) 1343, 1546, 1696
MroXI GAANNNNTTC 2 cut(s) 11, 767
MseI TTAA 1 cut(s) 1137
MslI CAYNNNNRTG 2 cut(s) 840, 929
MspI CCGG 1 cut(s) 850
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 1 cut(s) 1149
Mva1269I GAATGC 1 cut(s) 1452
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 1160, 1169
NarI GGCGCC 1 cut(s) 519
NcoI CCATGG 2 cut(s) 886, 1328
NlaIV GGNNCC 6 cut(s) 76, 133, 520, 531, 898, 1097
NmuCI GTSAC 4 cut(s) 706, 1427, 1486, 1745
NsbI TGCGCA 1 cut(s) 333
NspV TTCGAA 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 1308
PagI TCATGA 2 cut(s) 771, 1540
PaqCI CACCTGC 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 1179
PctI GAATGC 1 cut(s) 1452
PdmI GAANNNNTTC 2 cut(s) 11, 767
PflFI GACNNNGTC 1 cut(s) 104
PflMI CCANNNNNTGG 1 cut(s) 1422
PkrI GCNGC 6 cut(s) 799, 880, 883, 1171, 1531, 1729
PleI GAGTC 1 cut(s) 761
PluTI GGCGCC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 761
PshAI GACNNNNGTC 1 cut(s) 59
PsiI TTATAA 2 cut(s) 261, 1499
Psp124BI GAGCTC 1 cut(s) 976
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 6 cut(s) 76, 133, 520, 531, 898, 1097
PspPI GGNCC 2 cut(s) 131, 1109
PstI CTGCAG 1 cut(s) 1171
PstNI CAGNNNCTG 1 cut(s) 1422
PsuI RGATCY 1 cut(s) 529
PsyI GACNNNGTC 1 cut(s) 104
RsaI GTAC 8 cut(s) 377, 464, 587, 641, 830, 863, 1186, 1334
RsaNI GTAC 8 cut(s) 376, 463, 586, 640, 829, 862, 1185, 1333
RseI CAYNNNNRTG 2 cut(s) 840, 929
SacI GAGCTC 1 cut(s) 976
SapI GCTCTTC 1 cut(s) 1179
SaqAI TTAA 1 cut(s) 1137
SatI GCNGC 6 cut(s) 798, 879, 882, 1170, 1530, 1728
Sau96I GGNCC 2 cut(s) 131, 1109
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 1 cut(s) 762
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 2 cut(s) 256, 976
SexAI ACCWGGT 1 cut(s) 187
SfaNI GCATC 1 cut(s) 562
SfcI CTRYAG 2 cut(s) 1167, 1447
SfoI GGCGCC 1 cut(s) 520
Sfr274I CTCGAG 1 cut(s) 1308
SfuI TTCGAA 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 1308
SmiMI CAYNNNNRTG 2 cut(s) 840, 929
SmlI CTYRAG 2 cut(s) 266, 1308
SmoI CTYRAG 2 cut(s) 266, 1308
SsiI CCGC 5 cut(s) 48, 85, 575, 1022, 1569
SspDI GGCGCC 1 cut(s) 518
SspI AATATT 2 cut(s) 455, 613
SspMI CTAG 2 cut(s) 245, 1623
SstI GAGCTC 1 cut(s) 976
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 3 cut(s) 318, 886, 1328
TaiI ACGT 3 cut(s) 105, 158, 570
TaqI TCGA 4 cut(s) 340, 510, 1309, 1577
TatI WGTACW 6 cut(s) 375, 462, 585, 639, 828, 861
Tru1I TTAA 1 cut(s) 1137
Tru9I TTAA 1 cut(s) 1137
TseFI GTSAC 4 cut(s) 706, 1427, 1486, 1745
TseI GCWGC 6 cut(s) 797, 878, 881, 1169, 1529, 1727
Tsp45I GTSAC 4 cut(s) 706, 1427, 1486, 1745
TspDTI ATGAA 8 cut(s) 17, 108, 571, 668, 760, 788, 824, 913
Tth111I GACNNNGTC 1 cut(s) 104
Van91I CCANNNNNTGG 1 cut(s) 1422
XapI RAATTY 5 cut(s) 483, 675, 697, 1078, 1314
XcmI CCANNNNNNNNNTGG 1 cut(s) 1326
XhoI CTCGAG 1 cut(s) 1308
XmnI GAANNNNTTC 2 cut(s) 11, 767
XspI CTAG 2 cut(s) 245, 1623
ZraI GACGTC 1 cut(s) 103
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.