MD03G1236800.v1.1

28 kDa heat- and acid-stable phosphoprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001725
Physical Location & Seq
Forward (+)
32210326 .. 32217133
6808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1236800.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGGAAGAGGAAAGTTCAAGGGCAAGCCCACCGGCCGCCGCCAGTTCTCCACTCACGAAGAGATGGTTGCTGGTTCCTCTGCCCGTCCGCGTGGTTTTAAAAGGGAAGAAGCCGAAGAGGAGGAAGAAGTAGAGTCTGAAGAGGAATCAGAAGAAGAACAGGAAGAATCCGAAAAACGGAAAGGCACGCAGGGAATTATTGAAATTGAGAATCCCAATCTAGTAAAACCAAAGAATGTGAAGGCTAAAAATGTCGATATTGAGAAAACAACTGAACTCTCAAGGCGTGAAAGAGAGGAGATAGAGAAGCAAAAGGCTCATGAGCGGTACATGAGGTTGCAGGAACAGGGAAAAACAGAACAAGCAAAAAAGGATTTAGAACGCTTAGCCATGATTCGCCAACAAAGGGCTGAGGCTGCTAAGAAGCGAGAGGAGGAAAAGGCTGGTCTAAAGAGCAGAAAAAAGGCGAGGGACGCAAGTGAAATAAAGTTATGGAGATGCCAAGTAATTGAATTGGGCTGCTTTCTTTTTAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

180

Amino Acids

20.95

Weight (kDa)

8.43

Isoelectric Point (pI)

78.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP28 PF10252 71 - 148 3e-29 Casein kinase substrate phosphoprotein PP28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 325
AccII CGCG 1 cut(s) 91
AciI CCGC 4 cut(s) 37, 40, 89, 325
AcoI YGGCCR 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 2 cut(s) 177, 406
AgsI TTSAA 3 cut(s) 19, 203, 512
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 416, 519
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 403, 506
BccI CCATC 1 cut(s) 58
BfaI CTAG 1 cut(s) 221
BisI GCNGC 4 cut(s) 37, 40, 417, 520
BlpI GCTNAGC 1 cut(s) 385
BlsI GCNGC 4 cut(s) 38, 41, 418, 521
BmiI GGNNCC 1 cut(s) 76
BmsI GCATC 1 cut(s) 488
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 385
BpuEI CTTGAG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 406
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 177, 406
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 3 cut(s) 134, 311, 446
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 2 cut(s) 403, 506
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 2 cut(s) 177, 406
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 1 cut(s) 36
Bsp1720I GCTNAGC 1 cut(s) 385
BspACI CCGC 4 cut(s) 37, 40, 89, 325
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 91
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 32
Bst6I CTCTTC 3 cut(s) 54, 111, 135
BstC8I GCNNGC 2 cut(s) 26, 188
BstDEI CTNAG 3 cut(s) 385, 411, 420
BstFNI CGCG 1 cut(s) 91
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 2 cut(s) 416, 473
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 2 cut(s) 403, 506
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 26, 188
CciI TCATGA 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 32
CseI GACGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 328
CviAII CATG 3 cut(s) 320, 331, 391
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 3 cut(s) 385, 411, 420
DraI TTTAAA 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 34
EagI CGGCCG 1 cut(s) 34
Eam1104I CTCTTC 3 cut(s) 54, 111, 135
EarI CTCTTC 3 cut(s) 54, 111, 135
EclXI CGGCCG 1 cut(s) 34
Eco52I CGGCCG 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 159
FaeI CATG 3 cut(s) 323, 334, 394
FaiI YATR 4 cut(s) 321, 332, 392, 493
FaqI GGGAC 1 cut(s) 485
FatI CATG 3 cut(s) 319, 330, 390
Fnu4HI GCNGC 4 cut(s) 37, 40, 417, 520
Fsp4HI GCNGC 4 cut(s) 37, 40, 417, 520
FspBI CTAG 1 cut(s) 221
GluI GCNGC 4 cut(s) 37, 40, 417, 520
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 482
Hin1II CATG 3 cut(s) 323, 334, 394
HinfI GANTC 5 cut(s) 134, 146, 167, 211, 394
HpaII CCGG 1 cut(s) 33
Hpy188I TCNGA 3 cut(s) 139, 151, 172
Hpy188III TCNNGA 2 cut(s) 56, 320
HpyAV CCTTC 1 cut(s) 235
HpyCH4V TGCA 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 473
HpyF3I CTNAG 3 cut(s) 385, 411, 420
Hsp92II CATG 3 cut(s) 323, 334, 394
LpnPI CCDG 8 cut(s) 46, 56, 57, 146, 176, 326, 332, 429
Lsp1109I GCAGC 2 cut(s) 403, 506
LweI GCATC 1 cut(s) 488
MaeI CTAG 1 cut(s) 221
MbiI CCGCTC 1 cut(s) 325
MboII GAAGA 9 cut(s) 18, 71, 119, 128, 137, 152, 164, 167, 176
MluCI AATT 4 cut(s) 195, 204, 507, 512
MlyI GAGTC 1 cut(s) 143
MseI TTAA 2 cut(s) 99, 531
MspI CCGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 2 cut(s) 416, 473
NlaIII CATG 3 cut(s) 323, 334, 394
NlaIV GGNNCC 1 cut(s) 76
PagI TCATGA 1 cut(s) 319
PfeI GAWTC 4 cut(s) 146, 167, 211, 394
PkrI GCNGC 4 cut(s) 38, 41, 418, 521
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 76
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 2 cut(s) 99, 531
SatI GCNGC 4 cut(s) 37, 40, 417, 520
SchI GAGTC 1 cut(s) 143
SetI ASST 1 cut(s) 338
SfaNI GCATC 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 4 cut(s) 195, 204, 507, 512
SsiI CCGC 4 cut(s) 37, 40, 89, 325
SspMI CTAG 1 cut(s) 221
TaqI TCGA 1 cut(s) 255
TasI AATT 4 cut(s) 195, 204, 507, 512
TauI GCSGC 2 cut(s) 39, 42
TfiI GAWTC 4 cut(s) 146, 167, 211, 394
Tru1I TTAA 2 cut(s) 99, 531
Tru9I TTAA 2 cut(s) 99, 531
TseI GCWGC 2 cut(s) 416, 519
TspGWI ACGGA 1 cut(s) 193
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.