Rorug05G0128600

28 kDa heat- and acid-stable phosphoprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001725
Physical Location & Seq
Reverse (-)
11449905 .. 11451690
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0128600.1

Sequence Viewer

Length: 1299 bp
ATGTCATCCCCGGCGCTATTTTCATACAAGAATCTTCTTTCCTTTGAGTTTGTGATTGTTCTTCGTTGTTGTCAAGTGGCATCCTATGGCTGCAATCCTCTTCTAATTCATAAGGAAACCACCATGAAAACCTTAGGCGTAAAGCTTCTTCCACTATTTTTCATCCTTCTCAACTCAATTAGATTCTTAAACAATTCAACTCCAAAACCAGAAGCTGTCATTACTCCGGTTCATGAATCTCATGTTCAAGCAGCAATTATTTGCTCGAAGCAACAAGGCATACAAGTGCTACTCTTGGAGAAGTGTATTACAAAATTGCAGAAAATAGCAAGGTCTGGATTTCCAGCAGGGTCTTGTACCACTGTTGGTGTGGGGGGATATATTAGTGGAGGTGGATTTGGTACCATATTCAGGAAATATGGTCTAGCAGCTGACAATGTTGTCGATGCTAGGATAGTCGACGTTAATGGAAAAGTTCTAGACAGGAAATCCATGGGAGAAGATTTGTTTTGGGCTATAAGAGGAGGGGAAAGCTCAAGCTTTGGAGTCATTCTTGCATGGAAACTTAAGACTGGTTCCAGTTCCCCCATCTGTGTTGCAGATAAAGCTGGCACCACTGGCGGCAAAACTATCTCAGTTTCATTTGATGCATTGTTCATTGGACCAGTTGAGAAACTTCTTCCTTTGATGCAAGACAATTTCCCAGACTTGAGTTTAAACCGCAGTGATTGCACTGAAATGAGCTGGATCGAATCTGTTATGCACTATGCTGGCTTCTCAAAAAGTGAACCTCTGGAAGTTTTGCTTAACCAGGCTCAACCGTCTAGATACTTCTTCAAAGCAAAATCAGACTATTTGACCAAACCCATTTCAGAAGCTGGCTTGGAAGGTTTATGGAAAAGACTACTTCAAGTAGAGACATCCCAGTTGATACTGTCACGCTACGGTGAAAAAATGAGTGAGATTTCTGATTCAGAAACTCCATTCCCACGTAGAAGAGGAAACATATTCAAAATCCAGTATTTGGTCACGTGGTATGATGAAAAGGAGACGAACATAAATATTGGCCGGCCGGATGAAGAAGCTTCCCCAAGAGCTGCCTATTTGAACTACAGGGATCTTGACTTGGGGAGGAACAACAATGATGGCAATACAAGCTATGCGAAAGCAAGCATTTGGGGTTTGAAGTATTTCAAGAGCAATTTCAGGAGACTTGTTCATGTTAAGATCTTGGTTGATCCTTGTAACTTCTTTAGAAATGAACAAAGCATCCCTGTGTTCCCATCTAGAGGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

432

Amino Acids

48.14

Weight (kDa)

9.1

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 113 - 160 3.1e-07 FAD binding domain
BBE PF08031 367 - 425 1.4e-17 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 2 cut(s) 401, 611
AccB7I CCANNNNNTGG 1 cut(s) 1024
AccI GTMKAC 1 cut(s) 459
AciI CCGC 2 cut(s) 621, 721
AclWI GGATC 3 cut(s) 755, 1125, 1232
AcoI YGGCCR 2 cut(s) 1066, 1070
AcvI CACGTG 1 cut(s) 1032
AfaI GTAC 2 cut(s) 358, 403
AfiI CCNNNNNNNGG 3 cut(s) 411, 1024, 1289
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 8 cut(s) 198, 248, 838, 911, 1012, 1108, 1186, 1195
AjnI CCWGG 1 cut(s) 810
AloI GAACNNNNNNTCC 2 cut(s) 1254, 1286
Alw26I GTCTC 3 cut(s) 911, 1043, 1204
AlwI GGATC 3 cut(s) 755, 1125, 1232
AlwNI CAGNNNCTG 2 cut(s) 215, 878
AoxI GGCC 2 cut(s) 1066, 1070
ApeKI GCWGC 4 cut(s) 90, 251, 428, 1097
Asp700I GAANNNNTTC 1 cut(s) 1190
Asp718I GGTACC 1 cut(s) 401
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 662
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 959
AvaII GGWCC 1 cut(s) 662
AxyI CCTNAGG 1 cut(s) 133
BanI GGYRCC 2 cut(s) 401, 611
BarI GAAGNNNNNNTAC 2 cut(s) 132, 164
BbrPI CACGTG 1 cut(s) 1032
BbvI GCAGC 4 cut(s) 77, 263, 440, 1084
BccI CCATC 3 cut(s) 596, 1139, 1291
BciT130I CCWGG 1 cut(s) 812
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 3 cut(s) 911, 1043, 1204
BfaI CTAG 5 cut(s) 425, 450, 479, 825, 1287
BfmI CTRYAG 1 cut(s) 1111
BfoI RGCGCY 1 cut(s) 17
BfrI CTTAAG 1 cut(s) 566
BglII AGATCT 1 cut(s) 1227
BisI GCNGC 5 cut(s) 91, 252, 429, 622, 1098
BlsI GCNGC 5 cut(s) 92, 253, 430, 623, 1099
Bme1390I CCNGG 2 cut(s) 11, 812
Bme18I GGWCC 1 cut(s) 662
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 3 cut(s) 403, 577, 613
BmrFI CCNGG 2 cut(s) 11, 812
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 5 cut(s) 89, 436, 637, 678, 1278
BmuI ACTGGG 1 cut(s) 919
BpuEI CTTGAG 2 cut(s) 520, 730
BpuMI CCSGG 1 cut(s) 11
BsaAI YACGTR 2 cut(s) 992, 1032
BsaJI CCNNGG 2 cut(s) 9, 492
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 3 cut(s) 411, 1024, 1289
Bse118I RCCGGY 1 cut(s) 1068
Bse1I ACTGG 6 cut(s) 577, 579, 622, 665, 925, 1018
Bse21I CCTNAGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 812
BseDI CCNNGG 2 cut(s) 9, 492
BseGI GGATG 6 cut(s) 5, 80, 162, 920, 1081, 1269
BseLI CCNNNNNNNGG 3 cut(s) 411, 1024, 1289
BseMII CTCAG 1 cut(s) 648
BseNI ACTGG 6 cut(s) 577, 579, 622, 665, 925, 1018
BseRI GAGGAG 1 cut(s) 537
BseX3I CGGCCG 1 cut(s) 1070
BseXI GCAGC 4 cut(s) 77, 263, 440, 1084
Bsh1285I CGRYCG 1 cut(s) 1073
BshFI GGCC 2 cut(s) 1068, 1072
BshNI GGYRCC 2 cut(s) 401, 611
BsiEI CGRYCG 1 cut(s) 1073
BsiSI CCGG 4 cut(s) 11, 227, 1069, 1073
BslI CCNNNNNNNGG 3 cut(s) 411, 1024, 1289
BsmAI GTCTC 3 cut(s) 911, 1043, 1204
BsmBI CGTCTC 1 cut(s) 1043
BsnI GGCC 2 cut(s) 1068, 1072
Bsp143I GATC 4 cut(s) 747, 1117, 1227, 1237
Bsp19I CCATGG 1 cut(s) 492
BspACI CCGC 2 cut(s) 621, 721
BspANI GGCC 2 cut(s) 1068, 1072
BspCNI CTCAG 1 cut(s) 647
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 3 cut(s) 403, 577, 613
BspPI GGATC 3 cut(s) 755, 1125, 1232
BspT107I GGYRCC 2 cut(s) 401, 611
BspTI CTTAAG 1 cut(s) 566
BsrFI RCCGGY 1 cut(s) 1068
BsrI ACTGG 6 cut(s) 577, 579, 622, 665, 925, 1018
BssAI RCCGGY 1 cut(s) 1068
BssECI CCNNGG 2 cut(s) 9, 492
BssMI GATC 4 cut(s) 747, 1117, 1227, 1237
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 812
Bst4CI ACNGT 4 cut(s) 364, 822, 936, 947
Bst6I CTCTTC 2 cut(s) 105, 991
BstAFI CTTAAG 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 992, 1032
BstC8I GCNNGC 5 cut(s) 610, 772, 880, 1070, 1171
BstDEI CTNAG 2 cut(s) 133, 634
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 6 cut(s) 5, 80, 162, 920, 1081, 1269
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 4 cut(s) 750, 1120, 1230, 1240
BstMAI GTCTC 3 cut(s) 911, 1043, 1204
BstMBI GATC 4 cut(s) 747, 1117, 1227, 1237
BstMCI CGRYCG 1 cut(s) 1073
BstMWI GCNNNNNNNGC 4 cut(s) 605, 618, 729, 1155
BstNI CCWGG 1 cut(s) 812
BstSCI CCNGG 2 cut(s) 9, 810
BstSFI CTRYAG 1 cut(s) 1111
BstV1I GCAGC 4 cut(s) 77, 263, 440, 1084
BstX2I RGATCY 2 cut(s) 1117, 1227
BstYI RGATCY 2 cut(s) 1117, 1227
BstZI CGGCCG 1 cut(s) 1070
Bsu36I CCTNAGG 1 cut(s) 133
BsuRI GGCC 2 cut(s) 1068, 1072
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 6 cut(s) 5, 80, 162, 920, 1081, 1269
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 4 cut(s) 360, 615, 730, 732
Cac8I GCNNGC 5 cut(s) 610, 772, 880, 1070, 1171
CaiI CAGNNNCTG 2 cut(s) 215, 878
CciI TCATGA 1 cut(s) 232
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 1068
Cfr13I GGNCC 1 cut(s) 662
Csp6I GTAC 2 cut(s) 357, 402
CviAII CATG 6 cut(s) 124, 233, 242, 493, 558, 1220
CviQI GTAC 2 cut(s) 357, 402
DdeI CTNAG 2 cut(s) 133, 634
DpnI GATC 4 cut(s) 749, 1119, 1229, 1239
DpnII GATC 4 cut(s) 747, 1117, 1227, 1237
DraI TTTAAA 1 cut(s) 717
DrdI GACNNNNNNGTC 1 cut(s) 440
DseDI GACNNNNNNGTC 1 cut(s) 440
EaeI YGGCCR 2 cut(s) 1066, 1070
EagI CGGCCG 1 cut(s) 1070
Eam1104I CTCTTC 2 cut(s) 105, 991
EarI CTCTTC 2 cut(s) 105, 991
EclXI CGGCCG 1 cut(s) 1070
Eco130I CCWWGG 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 662
Eco52I CGGCCG 1 cut(s) 1070
Eco72I CACGTG 1 cut(s) 1032
Eco81I CCTNAGG 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 810
EcoT14I CCWWGG 1 cut(s) 492
EcoT22I ATGCAT 1 cut(s) 652
ErhI CCWWGG 1 cut(s) 492
Esp3I CGTCTC 1 cut(s) 1043
FaeI CATG 6 cut(s) 127, 236, 245, 496, 561, 1223
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FatI CATG 6 cut(s) 123, 232, 241, 492, 557, 1219
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 5 cut(s) 91, 252, 429, 622, 1098
FokI GGATG 5 cut(s) 67, 149, 907, 1088, 1256
FseI GGCCGGCC 1 cut(s) 1072
Fsp4HI GCNGC 5 cut(s) 91, 252, 429, 622, 1098
FspBI CTAG 5 cut(s) 425, 450, 479, 825, 1287
GlaI GCGC 1 cut(s) 15
GluI GCNGC 5 cut(s) 91, 252, 429, 622, 1098
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 2 cut(s) 1068, 1072
HapII CCGG 4 cut(s) 11, 227, 1069, 1073
HhaI GCGC 1 cut(s) 16
Hin1II CATG 6 cut(s) 127, 236, 245, 496, 561, 1223
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 3 cut(s) 143, 538, 1083
HinfI GANTC 6 cut(s) 31, 183, 236, 546, 752, 971
HpaII CCGG 4 cut(s) 11, 227, 1069, 1073
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 2 cut(s) 460, 788
Hpy188I TCNGA 4 cut(s) 850, 874, 970, 976
Hpy8I GTNNAC 2 cut(s) 460, 788
Hpy99I CGWCG 1 cut(s) 464
HpyAV CCTTC 2 cut(s) 176, 881
HpyCH4III ACNGT 4 cut(s) 364, 822, 936, 947
HpyCH4IV ACGT 3 cut(s) 462, 991, 1031
HpyCH4V TGCA 8 cut(s) 93, 319, 557, 599, 650, 691, 732, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 605, 618, 729, 1155
HpyF3I CTNAG 2 cut(s) 133, 634
HpySE526I ACGT 3 cut(s) 462, 991, 1031
Hsp92II CATG 6 cut(s) 127, 236, 245, 496, 561, 1223
HspAI GCGC 1 cut(s) 14
KpnI GGTACC 1 cut(s) 405
KroI GCCGGC 1 cut(s) 1068
KroNI GCCGGC 1 cut(s) 1070
Kzo9I GATC 4 cut(s) 747, 1117, 1227, 1237
Lsp1109I GCAGC 4 cut(s) 77, 263, 440, 1084
LweI GCATC 5 cut(s) 89, 436, 637, 678, 1278
MaeI CTAG 5 cut(s) 425, 450, 479, 825, 1287
MaeII ACGT 3 cut(s) 462, 991, 1031
MaeIII GTNAC 3 cut(s) 936, 1027, 1244
MalI GATC 4 cut(s) 749, 1119, 1229, 1239
MboI GATC 4 cut(s) 747, 1117, 1227, 1237
MboII GAAGA 9 cut(s) 26, 53, 92, 140, 512, 671, 826, 1008, 1091
MflI RGATCY 2 cut(s) 1117, 1227
MluCI AATT 7 cut(s) 105, 177, 193, 255, 314, 697, 1201
MlyI GAGTC 1 cut(s) 555
MnlI CCTC 8 cut(s) 108, 383, 515, 518, 801, 992, 1125, 1283
Mph1103I ATGCAT 1 cut(s) 652
MroNI GCCGGC 1 cut(s) 1068
MroXI GAANNNNTTC 1 cut(s) 1190
MseI TTAA 6 cut(s) 188, 465, 567, 716, 807, 1224
MslI CAYNNNNRTG 3 cut(s) 284, 737, 1274
MspA1I CMGCKG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 4 cut(s) 11, 227, 1069, 1073
MspR9I CCNGG 2 cut(s) 11, 812
MssI GTTTAAAC 1 cut(s) 717
MvaI CCWGG 1 cut(s) 812
MwoI GCNNNNNNNGC 4 cut(s) 605, 618, 729, 1155
NaeI GCCGGC 1 cut(s) 1070
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 492
NdeII GATC 4 cut(s) 747, 1117, 1227, 1237
NgoMIV GCCGGC 1 cut(s) 1068
NlaIII CATG 6 cut(s) 127, 236, 245, 496, 561, 1223
NlaIV GGNNCC 3 cut(s) 403, 577, 613
NmuCI GTSAC 2 cut(s) 936, 1027
NsiI ATGCAT 1 cut(s) 652
PagI TCATGA 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 1070
PdmI GAANNNNTTC 1 cut(s) 1190
PfeI GAWTC 5 cut(s) 31, 183, 236, 752, 971
PflMI CCANNNNNTGG 1 cut(s) 1024
PkrI GCNGC 5 cut(s) 92, 253, 430, 623, 1099
PleI GAGTC 1 cut(s) 554
PmaCI CACGTG 1 cut(s) 1032
PmeI GTTTAAAC 1 cut(s) 717
PmlI CACGTG 1 cut(s) 1032
PpsI GAGTC 1 cut(s) 554
Ppu21I YACGTR 2 cut(s) 992, 1032
Psp6I CCWGG 1 cut(s) 810
PspCI CACGTG 1 cut(s) 1032
PspGI CCWGG 1 cut(s) 810
PspN4I GGNNCC 3 cut(s) 403, 577, 613
PspPI GGNCC 1 cut(s) 662
PstNI CAGNNNCTG 2 cut(s) 215, 878
PsuI RGATCY 2 cut(s) 1117, 1227
PvuII CAGCTG 1 cut(s) 431
RigI GGCCGGCC 1 cut(s) 1072
RsaI GTAC 2 cut(s) 358, 403
RsaNI GTAC 2 cut(s) 357, 402
RseI CAYNNNNRTG 3 cut(s) 284, 737, 1274
SalI GTCGAC 1 cut(s) 458
SaqAI TTAA 6 cut(s) 188, 465, 567, 716, 807, 1224
SatI GCNGC 5 cut(s) 91, 252, 429, 622, 1098
Sau3AI GATC 4 cut(s) 747, 1117, 1227, 1237
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 2 cut(s) 11, 812
SfaNI GCATC 5 cut(s) 89, 436, 637, 678, 1278
SfcI CTRYAG 1 cut(s) 1111
SinI GGWCC 1 cut(s) 662
SmiMI CAYNNNNRTG 3 cut(s) 284, 737, 1274
SmlI CTYRAG 3 cut(s) 535, 566, 709
SmoI CTYRAG 3 cut(s) 535, 566, 709
Sse9I AATT 7 cut(s) 105, 177, 193, 255, 314, 697, 1201
SsiI CCGC 2 cut(s) 621, 721
SspI AATATT 1 cut(s) 1063
SspMI CTAG 5 cut(s) 425, 450, 479, 825, 1287
StyD4I CCNGG 2 cut(s) 9, 810
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 4 cut(s) 364, 822, 936, 947
TaiI ACGT 3 cut(s) 465, 994, 1034
TaqI TCGA 4 cut(s) 266, 444, 459, 750
TasI AATT 7 cut(s) 105, 177, 193, 255, 314, 697, 1201
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 5 cut(s) 31, 183, 236, 752, 971
Tru1I TTAA 6 cut(s) 188, 465, 567, 716, 807, 1224
Tru9I TTAA 6 cut(s) 188, 465, 567, 716, 807, 1224
TscAI CASTG 4 cut(s) 367, 622, 730, 739
TseFI GTSAC 2 cut(s) 936, 1027
TseI GCWGC 4 cut(s) 90, 251, 428, 1097
Tsp45I GTSAC 2 cut(s) 936, 1027
TspRI CASTG 4 cut(s) 367, 622, 730, 739
Van91I CCANNNNNTGG 1 cut(s) 1024
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 662
XbaI TCTAGA 3 cut(s) 478, 824, 1286
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 1190
XspI CTAG 5 cut(s) 425, 450, 479, 825, 1287
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.