28 kDa heat- and acid-stable phosphoprotein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 49, 170 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 334, 366 |
| AluBI | AGCT | 2 cut(s) | 34, 350 |
| AluI | AGCT | 2 cut(s) | 34, 350 |
| Alw21I | GWGCWC | 1 cut(s) | 14 |
| ApeKI | GCWGC | 2 cut(s) | 282, 308 |
| Bbv12I | GWGCWC | 1 cut(s) | 14 |
| BbvI | GCAGC | 2 cut(s) | 294, 320 |
| BceAI | ACGGC | 1 cut(s) | 372 |
| BfaI | CTAG | 2 cut(s) | 86, 147 |
| BfmI | CTRYAG | 1 cut(s) | 379 |
| BisI | GCNGC | 2 cut(s) | 283, 309 |
| BlsI | GCNGC | 2 cut(s) | 284, 310 |
| BpmI | CTGGAG | 1 cut(s) | 20 |
| BpuEI | CTTGAG | 1 cut(s) | 146 |
| BsaJI | CCNNGG | 1 cut(s) | 341 |
| BseDI | CCNNGG | 1 cut(s) | 341 |
| BseRI | GAGGAG | 1 cut(s) | 176 |
| BseXI | GCAGC | 2 cut(s) | 294, 320 |
| BsiHKAI | GWGCWC | 1 cut(s) | 14 |
| BsmI | GAATGC | 1 cut(s) | 245 |
| Bsp1286I | GDGCHC | 1 cut(s) | 14 |
| Bsp143I | GATC | 1 cut(s) | 121 |
| BspHI | TCATGA | 1 cut(s) | 184 |
| BspMAI | CTGCAG | 1 cut(s) | 383 |
| BssECI | CCNNGG | 1 cut(s) | 341 |
| BssMI | GATC | 1 cut(s) | 121 |
| BssT1I | CCWWGG | 1 cut(s) | 341 |
| Bst4CI | ACNGT | 1 cut(s) | 322 |
| BstC8I | GCNNGC | 1 cut(s) | 280 |
| BstKTI | GATC | 1 cut(s) | 124 |
| BstMBI | GATC | 1 cut(s) | 121 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 356 |
| BstSFI | CTRYAG | 1 cut(s) | 379 |
| BstV1I | GCAGC | 2 cut(s) | 294, 320 |
| BtsIMutI | CAGTG | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 1 cut(s) | 280 |
| CciI | TCATGA | 1 cut(s) | 184 |
| CviAII | CATG | 2 cut(s) | 185, 196 |
| CviJI | RGCY | 7 cut(s) | 34, 94, 110, 182, 311, 350, 359 |
| CviKI_1 | RGCY | 7 cut(s) | 34, 94, 110, 182, 311, 350, 359 |
| DpnI | GATC | 1 cut(s) | 123 |
| DpnII | GATC | 1 cut(s) | 121 |
| Eco130I | CCWWGG | 1 cut(s) | 341 |
| EcoT14I | CCWWGG | 1 cut(s) | 341 |
| ErhI | CCWWGG | 1 cut(s) | 341 |
| FaeI | CATG | 2 cut(s) | 188, 199 |
| FaiI | YATR | 5 cut(s) | 65, 186, 197, 362, 402 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 334, 366 |
| FatI | CATG | 2 cut(s) | 184, 195 |
| Fnu4HI | GCNGC | 2 cut(s) | 283, 309 |
| Fsp4HI | GCNGC | 2 cut(s) | 283, 309 |
| FspBI | CTAG | 2 cut(s) | 86, 147 |
| GluI | GCNGC | 2 cut(s) | 283, 309 |
| GsuI | CTGGAG | 1 cut(s) | 20 |
| Hin1II | CATG | 2 cut(s) | 188, 199 |
| HincII | GTYRAC | 1 cut(s) | 338 |
| HindII | GTYRAC | 1 cut(s) | 338 |
| HindIII | AAGCTT | 1 cut(s) | 348 |
| HinfI | GANTC | 5 cut(s) | 40, 60, 76, 334, 375 |
| Hpy166II | GTNNAC | 1 cut(s) | 338 |
| Hpy188III | TCNNGA | 3 cut(s) | 37, 125, 185 |
| Hpy8I | GTNNAC | 1 cut(s) | 338 |
| HpyAV | CCTTC | 2 cut(s) | 100, 149 |
| HpyCH4III | ACNGT | 1 cut(s) | 322 |
| HpyCH4V | TGCA | 2 cut(s) | 205, 381 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 356 |
| Hsp92II | CATG | 2 cut(s) | 188, 199 |
| Kzo9I | GATC | 1 cut(s) | 121 |
| LmnI | GCTCC | 2 cut(s) | 39, 208 |
| LpnPI | CCDG | 5 cut(s) | 50, 191, 197, 235, 367 |
| Lsp1109I | GCAGC | 2 cut(s) | 294, 320 |
| MaeI | CTAG | 2 cut(s) | 86, 147 |
| MalI | GATC | 1 cut(s) | 123 |
| MboI | GATC | 1 cut(s) | 121 |
| MhlI | GDGCHC | 1 cut(s) | 14 |
| MluCI | AATT | 4 cut(s) | 24, 69, 329, 386 |
| MlyI | GAGTC | 2 cut(s) | 343, 384 |
| MnlI | CCTC | 2 cut(s) | 154, 192 |
| MseI | TTAA | 1 cut(s) | 273 |
| Mva1269I | GAATGC | 1 cut(s) | 245 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 356 |
| NdeII | GATC | 1 cut(s) | 121 |
| NlaIII | CATG | 2 cut(s) | 188, 199 |
| PagI | TCATGA | 1 cut(s) | 184 |
| PctI | GAATGC | 1 cut(s) | 245 |
| PfeI | GAWTC | 3 cut(s) | 40, 60, 76 |
| PkrI | GCNGC | 2 cut(s) | 284, 310 |
| PleI | GAGTC | 2 cut(s) | 342, 383 |
| PpsI | GAGTC | 2 cut(s) | 342, 383 |
| PstI | CTGCAG | 1 cut(s) | 383 |
| SaqAI | TTAA | 1 cut(s) | 273 |
| SatI | GCNGC | 2 cut(s) | 283, 309 |
| Sau3AI | GATC | 1 cut(s) | 121 |
| SchI | GAGTC | 2 cut(s) | 343, 384 |
| SduI | GDGCHC | 1 cut(s) | 14 |
| SetI | ASST | 4 cut(s) | 36, 203, 254, 352 |
| SfcI | CTRYAG | 1 cut(s) | 379 |
| SmlI | CTYRAG | 1 cut(s) | 125 |
| SmoI | CTYRAG | 1 cut(s) | 125 |
| Sse9I | AATT | 4 cut(s) | 24, 69, 329, 386 |
| SspMI | CTAG | 2 cut(s) | 86, 147 |
| StyI | CCWWGG | 1 cut(s) | 341 |
| TaaI | ACNGT | 1 cut(s) | 322 |
| TaqI | TCGA | 2 cut(s) | 17, 120 |
| TasI | AATT | 4 cut(s) | 24, 69, 329, 386 |
| TfiI | GAWTC | 3 cut(s) | 40, 60, 76 |
| Tru1I | TTAA | 1 cut(s) | 273 |
| Tru9I | TTAA | 1 cut(s) | 273 |
| TscAI | CASTG | 1 cut(s) | 325 |
| TseI | GCWGC | 2 cut(s) | 282, 308 |
| TspRI | CASTG | 1 cut(s) | 325 |
| XspI | CTAG | 2 cut(s) | 86, 147 |