pycom10g24000

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001725
Physical Location & Seq
Reverse (-)
25926686 .. 25928778
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24000.5

Sequence Viewer

Length: 498 bp
ATGGGAAGGGGGAAGTACAAGAGCAAGCCCACCGGCCATCGGCAGTTCTCCACTCCCGAAGATCTTCTTGCTGGTACCTCAAGCCGTCCGCAGACTTTTTCAAAGCGAGAAGCTGAGCGTGATGAAGTTGAAGTAGATTTCGTGGCAAGTTCCGAAGAGGAGTCTGGAGATGAATCCGAAGGACATGAGGAGAAGAAGAAGGGGATCCAAGGGATTATTGAGATTGAAAATCCGAATTTGGTAAAGTCGAAGACCGTGAAAGCTCGAGACGTTAATATGGGAAGGACAACCGAACTCTCAAGGCGTGAAAGAGAAGAATTGGAGAAACAGAGAGCCCGCGAGAGACATATGAGGCTGCAAGAACAGGGAAAAACTGAACAAGCAAGGAAAGATTTAGAGCGTTTAGCGCTTATACGAGAGCAAAGGGCGGAAGCTGCTAAAAAGAGGGAAGAAGAAAAAGCTGGTGGCTTAGGAAAAAATAAAATAACAGCCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

166

Amino Acids

18.84

Weight (kDa)

9.07

Isoelectric Point (pI)

58.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 74
AccII CGCG 1 cut(s) 339
AciI CCGC 3 cut(s) 89, 337, 428
AclWI GGATC 2 cut(s) 199, 212
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 235
AfaI GTAC 2 cut(s) 17, 76
AfeI AGCGCT 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 3 cut(s) 102, 131, 227
AluBI AGCT 4 cut(s) 113, 263, 434, 461
AluI AGCT 4 cut(s) 113, 263, 434, 461
Alw26I GTCTC 2 cut(s) 261, 337
AlwI GGATC 2 cut(s) 199, 212
Ama87I CYCGRG 1 cut(s) 264
Aor51HI AGCGCT 1 cut(s) 408
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 355, 434
ApoI RAATTY 1 cut(s) 235
Asp700I GAANNNNTTC 1 cut(s) 63
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 409
AvaI CYCGRG 1 cut(s) 264
BamHI GGATCC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 257
BbvI GCAGC 2 cut(s) 342, 421
BccI CCATC 1 cut(s) 45
BceAI ACGGC 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 261, 337
BfoI RGCGCY 1 cut(s) 410
BglII AGATCT 1 cut(s) 61
BisI GCNGC 2 cut(s) 356, 435
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 357, 436
BmeT110I CYCGRG 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 76, 206
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 469
Bpu1102I GCTNAGC 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 64, 283
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse118I RCCGGY 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 105
BseRI GAGGAG 2 cut(s) 173, 203
BseXI GCAGC 2 cut(s) 342, 421
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 2 cut(s) 261, 337
BsmBI CGTCTC 1 cut(s) 261
BsnI GGCC 1 cut(s) 36
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 2 cut(s) 61, 204
Bsp1720I GCTNAGC 1 cut(s) 114
BspACI CCGC 3 cut(s) 89, 337, 428
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 1 cut(s) 106
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 76, 206
BspPI GGATC 2 cut(s) 199, 212
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 2 cut(s) 61, 204
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 26, 337
BstDEI CTNAG 2 cut(s) 114, 469
BstFNI CGCG 1 cut(s) 339
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 2 cut(s) 64, 207
BstMAI GTCTC 2 cut(s) 261, 337
BstMBI GATC 2 cut(s) 61, 204
BstMWI GCNNNNNNNGC 2 cut(s) 406, 434
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 342, 421
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 2 cut(s) 61, 204
BstYI RGATCY 2 cut(s) 61, 204
BsuRI GGCC 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 26, 337
CfoI GCGC 1 cut(s) 409
Cfr10I RCCGGY 1 cut(s) 32
Csp6I GTAC 2 cut(s) 16, 75
CviAII CATG 1 cut(s) 185
CviQI GTAC 2 cut(s) 16, 75
DdeI CTNAG 2 cut(s) 114, 469
DpnI GATC 2 cut(s) 63, 206
DpnII GATC 2 cut(s) 61, 204
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 337
Eco47III AGCGCT 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 1 cut(s) 188
FaiI YATR 5 cut(s) 186, 278, 348, 350, 413
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FatI CATG 1 cut(s) 184
FauI CCCGC 1 cut(s) 344
FauNDI CATATG 1 cut(s) 348
Fnu4HI GCNGC 2 cut(s) 356, 435
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 2 cut(s) 356, 435
GlaI GCGC 1 cut(s) 408
GluI GCNGC 2 cut(s) 356, 435
GsuI CTGGAG 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 33
HhaI GCGC 1 cut(s) 409
Hin1II CATG 1 cut(s) 188
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HinfI GANTC 2 cut(s) 161, 173
HpaII CCGG 1 cut(s) 33
Hpy188I TCNGA 3 cut(s) 154, 178, 234
Hpy188III TCNNGA 3 cut(s) 56, 165, 266
HpyAV CCTTC 3 cut(s) 173, 193, 276
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 358
HpyF10VI GCNNNNNNNGC 2 cut(s) 406, 434
HpyF3I CTNAG 2 cut(s) 114, 469
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 188
HspAI GCGC 1 cut(s) 407
KpnI GGTACC 1 cut(s) 78
Kzo9I GATC 2 cut(s) 61, 204
LpnPI CCDG 5 cut(s) 46, 57, 150, 350, 447
Lsp1109I GCAGC 2 cut(s) 342, 421
MaeII ACGT 1 cut(s) 270
MalI GATC 2 cut(s) 63, 206
MboI GATC 2 cut(s) 61, 204
MboII GAAGA 9 cut(s) 56, 71, 167, 205, 208, 262, 326, 461, 464
MflI RGATCY 2 cut(s) 61, 204
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 2 cut(s) 235, 317
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 5 cut(s) 88, 151, 181, 345, 438
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 1 cut(s) 273
MspI CCGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 406, 434
NdeI CATATG 1 cut(s) 348
NdeII GATC 2 cut(s) 61, 204
NlaIII CATG 1 cut(s) 188
NlaIV GGNNCC 2 cut(s) 76, 206
PaeR7I CTCGAG 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 357, 436
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 76, 206
PsuI RGATCY 2 cut(s) 61, 204
RsaI GTAC 2 cut(s) 17, 76
RsaNI GTAC 2 cut(s) 16, 75
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 2 cut(s) 356, 435
Sau3AI GATC 2 cut(s) 61, 204
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 1 cut(s) 337
SetI ASST 6 cut(s) 80, 115, 265, 273, 436, 463
Sfr274I CTCGAG 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 264
SmlI CTYRAG 3 cut(s) 79, 264, 298
SmoI CTYRAG 3 cut(s) 79, 264, 298
Sse9I AATT 2 cut(s) 235, 317
SsiI CCGC 3 cut(s) 89, 337, 428
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 248, 265
TasI AATT 2 cut(s) 235, 317
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TseI GCWGC 2 cut(s) 355, 434
TspDTI ATGAA 2 cut(s) 138, 186
XapI RAATTY 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.