RchiOBHm_Chr5g0031891

28 kDa heat- and acid-stable phosphoprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001725
Physical Location & Seq
Reverse (-)
25647975 .. 25652199
4225 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31110

Sequence Viewer

Length: 681 bp
ATGGACGTAAAAGGCTCGGCTCGTACAGTAGGTCTTCAAGTGCAAAAGCCAAAAGCATCAAGAAGAAGAAGAAGCAGCGGCAGCAGCAGCAGCAGCTATAAATTACAAAAACCCTTCCCCTCCTCAGCTTTCCTCCTCCTCCTTCTTCTTCTTCTTCTTCTCCTCCGATCTCTCTCTCTCTCTCTGTTATCTCTTGAATCTAAAATGGGAAGAGGAAAGTTCAAGGGCAAGCCCACCGGTCACCGCCACTTCTCCACTCACGAAGAGATGGTTGCTGGTACCTCTGCCCGGCCACGTAGCTTTAGAAGGGAAGAAGCTGAAGAGGAGGAAGAAGTAGAGTCTGAAGAGGAATCATCAGAAGAAGAAGAAGCAGAAAAGCGGAAGGGTACTCAGGGAATCATAGAAATTGAGAATCCCAATCTAGTAAAGCCAAAGAATGTGAAGGCTAAAAATGTCGATCTTGAGAAAACAACTGAACTTTCTAGGCGTGAAAGGGAGGAGATTGAAAAACAAAAGGCTCATGAGCGATACATGAGGTTGCAGGAGCAGGGAAAAACTGAACAAGCAAAGAAAGATTTAGAACGTTTAGCCATGATTCGCCAACAAAGGGCAGAGGCTGCTAAGAAGCGAGAAGAGGAGAAGGCTGCCAAGGAACAGAAAAAAGGCGAGGGACGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

226

Amino Acids

25.9

Weight (kDa)

9.69

Isoelectric Point (pI)

80.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP28 PF10252 138 - 215 1.1e-28 Casein kinase substrate phosphoprotein PP28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 3 cut(s) 78, 244, 379
AclI AACGTT 1 cut(s) 583
AcoI YGGCCR 1 cut(s) 290
AcuI CTGAAG 2 cut(s) 339, 363
AfaI GTAC 3 cut(s) 25, 280, 388
AfiI CCNNNNNNNGG 2 cut(s) 288, 607
AgeI ACCGGT 1 cut(s) 236
AgsI TTSAA 4 cut(s) 38, 197, 223, 506
AluBI AGCT 4 cut(s) 96, 128, 300, 317
AluI AGCT 4 cut(s) 96, 128, 300, 317
AlwNI CAGNNNCTG 1 cut(s) 617
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 8 cut(s) 75, 81, 84, 87, 90, 93, 617, 644
AsiGI ACCGGT 1 cut(s) 236
Asp718I GGTACC 1 cut(s) 278
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 1 cut(s) 233
BanI GGYRCC 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 26
BbvCI CCTCAGC 1 cut(s) 124
BbvI GCAGC 8 cut(s) 87, 93, 96, 99, 102, 105, 604, 631
BccI CCATC 1 cut(s) 262
BcnI CCSGG 1 cut(s) 289
BfaI CTAG 2 cut(s) 422, 483
BisI GCNGC 9 cut(s) 76, 79, 82, 85, 88, 91, 94, 618, 645
BlsI GCNGC 9 cut(s) 77, 80, 83, 86, 89, 92, 95, 619, 646
Bme1390I CCNGG 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 289
BsaAI YACGTR 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 648
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 607
Bse118I RCCGGY 1 cut(s) 236
BseDI CCNNGG 1 cut(s) 648
BseLI CCNNNNNNNGG 2 cut(s) 288, 607
BseMII CTCAG 2 cut(s) 138, 404
BseRI GAGGAG 7 cut(s) 112, 125, 128, 152, 338, 512, 650
BseXI GCAGC 8 cut(s) 87, 93, 96, 99, 102, 105, 604, 631
BshFI GGCC 1 cut(s) 292
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 236
BsiSI CCGG 2 cut(s) 237, 289
BslI CCNNNNNNNGG 2 cut(s) 288, 607
BsnI GGCC 1 cut(s) 292
Bsp143I GATC 2 cut(s) 167, 457
BspACI CCGC 3 cut(s) 78, 244, 379
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 2 cut(s) 137, 403
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 648
BssMI GATC 2 cut(s) 167, 457
BssT1I CCWWGG 1 cut(s) 648
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 5 cut(s) 205, 258, 315, 339, 627
BstAPI GCANNNNNTGC 1 cut(s) 617
BstBAI YACGTR 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 3 cut(s) 124, 390, 621
BstEII GGTNACC 1 cut(s) 239
BstKTI GATC 2 cut(s) 170, 460
BstMBI GATC 2 cut(s) 167, 457
BstMWI GCNNNNNNNGC 7 cut(s) 81, 84, 87, 90, 93, 617, 672
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 287
BstV1I GCAGC 8 cut(s) 87, 93, 96, 99, 102, 105, 604, 631
BstV2I GAAGAC 1 cut(s) 26
BsuRI GGCC 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 230
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 520
Cfr10I RCCGGY 1 cut(s) 236
Csp6I GTAC 3 cut(s) 24, 279, 387
CspAI ACCGGT 1 cut(s) 236
CviAII CATG 3 cut(s) 521, 532, 592
CviQI GTAC 3 cut(s) 24, 279, 387
DdeI CTNAG 3 cut(s) 124, 390, 621
DpnI GATC 2 cut(s) 169, 459
DpnII GATC 2 cut(s) 167, 457
EaeI YGGCCR 1 cut(s) 290
Eam1104I CTCTTC 5 cut(s) 205, 258, 315, 339, 627
EarI CTCTTC 5 cut(s) 205, 258, 315, 339, 627
Eco130I CCWWGG 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 339, 363
Eco91I GGTNACC 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 648
FaeI CATG 3 cut(s) 524, 535, 595
FaiI YATR 5 cut(s) 99, 401, 522, 533, 593
FatI CATG 3 cut(s) 520, 531, 591
Fnu4HI GCNGC 9 cut(s) 76, 79, 82, 85, 88, 91, 94, 618, 645
Fsp4HI GCNGC 9 cut(s) 76, 79, 82, 85, 88, 91, 94, 618, 645
FspBI CTAG 2 cut(s) 422, 483
GluI GCNGC 9 cut(s) 76, 79, 82, 85, 88, 91, 94, 618, 645
HaeIII GGCC 1 cut(s) 292
HapII CCGG 2 cut(s) 237, 289
Hin1II CATG 3 cut(s) 524, 535, 595
HinfI GANTC 6 cut(s) 197, 338, 350, 396, 412, 595
HpaII CCGG 2 cut(s) 237, 289
HphI GGTGA 1 cut(s) 233
Hpy188I TCNGA 3 cut(s) 167, 343, 358
Hpy188III TCNNGA 5 cut(s) 60, 194, 260, 461, 521
HpyAV CCTTC 6 cut(s) 124, 152, 300, 376, 436, 634
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 3 cut(s) 6, 295, 583
HpyCH4V TGCA 2 cut(s) 43, 541
HpyF10VI GCNNNNNNNGC 7 cut(s) 81, 84, 87, 90, 93, 617, 672
HpyF3I CTNAG 3 cut(s) 124, 390, 621
HpySE526I ACGT 3 cut(s) 6, 295, 583
Hsp92II CATG 3 cut(s) 524, 535, 595
KpnI GGTACC 1 cut(s) 282
Kzo9I GATC 2 cut(s) 167, 457
LmnI GCTCC 1 cut(s) 544
LpnPI CCDG 6 cut(s) 250, 261, 302, 377, 527, 533
Lsp1109I GCAGC 8 cut(s) 87, 93, 96, 99, 102, 105, 604, 631
LweI GCATC 1 cut(s) 65
MaeI CTAG 2 cut(s) 422, 483
MaeII ACGT 3 cut(s) 6, 295, 583
MaeIII GTNAC 1 cut(s) 239
MalI GATC 2 cut(s) 169, 459
MboI GATC 2 cut(s) 167, 457
MluCI AATT 2 cut(s) 101, 405
MlyI GAGTC 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 2 cut(s) 237, 289
MspR9I CCNGG 1 cut(s) 289
MwoI GCNNNNNNNGC 7 cut(s) 81, 84, 87, 90, 93, 617, 672
NciI CCSGG 1 cut(s) 289
NdeII GATC 2 cut(s) 167, 457
NlaIII CATG 3 cut(s) 524, 535, 595
NlaIV GGNNCC 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 239
PagI TCATGA 1 cut(s) 520
PfeI GAWTC 5 cut(s) 197, 350, 396, 412, 595
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 9 cut(s) 77, 80, 83, 86, 89, 92, 95, 619, 646
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Ppu21I YACGTR 1 cut(s) 296
Psp1406I AACGTT 1 cut(s) 583
PspEI GGTNACC 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 617
RsaI GTAC 3 cut(s) 25, 280, 388
RsaNI GTAC 3 cut(s) 24, 279, 387
SatI GCNGC 9 cut(s) 76, 79, 82, 85, 88, 91, 94, 618, 645
Sau3AI GATC 2 cut(s) 167, 457
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 289
SfaNI GCATC 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 2 cut(s) 101, 405
SsiI CCGC 3 cut(s) 78, 244, 379
SspMI CTAG 2 cut(s) 422, 483
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 3 cut(s) 9, 298, 586
TaqI TCGA 1 cut(s) 456
TasI AATT 2 cut(s) 101, 405
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 5 cut(s) 197, 350, 396, 412, 595
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 8 cut(s) 75, 81, 84, 87, 90, 93, 617, 644
Tsp45I GTSAC 1 cut(s) 239
XspI CTAG 2 cut(s) 422, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.