pycom05g28430

28 kDa heat- and acid-stable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001725
Physical Location & Seq
Reverse (-)
29162276 .. 29164054
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28430.6

Sequence Viewer

Length: 513 bp
ATGGGAAGGGGGAAGTTCAAGAGCAAGCCCACCGGCCATCGCCAGTTCTCCACTCCCGAAGATCTTCTTGCTGGTACCTCCAGCCGTCCTCGAACTTTTTCGAAGCGAGAAGCTGAACATGATGAAGTTGAAGTAGATTTTGTAGCAAGTTCCGAAGAGGAGTCCGGAGATGAATCCGAAGGACAAGATGAGAAGAAGAAGGGGATCCAAGGGATTATTGAGATTGAAAATCCGAATTTGGTAAAGCCAAAGACTGTGAAAGCTCGAGACGTTGATATGGGAAGGACAACTGAACTCTCAAGGCGCGAAAGAGAAGAAATAGAGAAACAGAGAGCCCGTGAGAGATATATGAGGTTGCAAGAACAGGGAAAAACTGAACAAGCAAGGAAAGATTTAGAGCGTTTAGCTCTTATACGAGAGCAAAGGGCAGAAGCTGCTAAAAAGAGAGAAGAAGAAAAAGCTGCCAAAGAACAGAAGAAGGTTGAAGCCCGCAAATCGATCGATCTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP28

Protein Analysis

171

Amino Acids

19.64

Weight (kDa)

8.71

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 74
AccII CGCG 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 1 cut(s) 490
AclWI GGATC 2 cut(s) 199, 212
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 235
AfaI GTAC 1 cut(s) 76
AgsI TTSAA 4 cut(s) 19, 131, 227, 485
AluBI AGCT 5 cut(s) 113, 263, 407, 434, 461
AluI AGCT 5 cut(s) 113, 263, 407, 434, 461
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 2 cut(s) 199, 212
AlwNI CAGNNNCTG 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 264
Aor13HI TCCGGA 1 cut(s) 164
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 434, 461
ApoI RAATTY 1 cut(s) 235
Asp700I GAANNNNTTC 2 cut(s) 63, 97
Asp718I GGTACC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 306
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 264
BamHI GGATCC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 337
BbvI GCAGC 2 cut(s) 421, 448
BccI CCATC 1 cut(s) 45
BceAI ACGGC 1 cut(s) 69
BcgI CGANNNNNNTGC 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 261
BglII AGATCT 1 cut(s) 61
BisI GCNGC 2 cut(s) 435, 462
BlsI GCNGC 2 cut(s) 436, 463
BmeT110I CYCGRG 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 76, 206
BpmI CTGGAG 1 cut(s) 64
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 283
Bsa29I ATCGAT 2 cut(s) 497, 501
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 1 cut(s) 164
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 164
BseCI ATCGAT 2 cut(s) 497, 501
BseDI CCNNGG 1 cut(s) 208
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 421, 448
Bsh1236I CGCG 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 74
BshVI ATCGAT 2 cut(s) 497, 501
BsiEI CGRYCG 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 2 cut(s) 33, 165
BsmAI GTCTC 1 cut(s) 261
BsmBI CGTCTC 1 cut(s) 261
BsnI GGCC 1 cut(s) 36
BsoBI CYCGRG 1 cut(s) 264
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 337
Bsp13I TCCGGA 1 cut(s) 164
Bsp143I GATC 4 cut(s) 61, 204, 498, 502
BspACI CCGC 1 cut(s) 490
BspANI GGCC 1 cut(s) 36
BspDI ATCGAT 2 cut(s) 497, 501
BspEI TCCGGA 1 cut(s) 164
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 76, 206
BspPI GGATC 2 cut(s) 199, 212
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 4 cut(s) 61, 204, 498, 502
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 2 cut(s) 26, 490
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 64, 207, 501, 505
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 4 cut(s) 61, 204, 498, 502
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 421, 448
BstX2I RGATCY 2 cut(s) 61, 204
BstYI RGATCY 2 cut(s) 61, 204
Bsu15I ATCGAT 2 cut(s) 497, 501
BsuRI GGCC 1 cut(s) 36
BsuTUI ATCGAT 2 cut(s) 497, 501
BtgZI GCGATG 1 cut(s) 23
Cac8I GCNNGC 2 cut(s) 26, 490
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 32
ClaI ATCGAT 2 cut(s) 497, 501
Csp6I GTAC 1 cut(s) 75
CviAII CATG 1 cut(s) 119
CviQI GTAC 1 cut(s) 75
DpnI GATC 4 cut(s) 63, 206, 500, 504
DpnII GATC 4 cut(s) 61, 204, 498, 502
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 1 cut(s) 122
FaiI YATR 5 cut(s) 120, 278, 348, 350, 413
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FatI CATG 1 cut(s) 118
FauI CCCGC 1 cut(s) 497
Fnu4HI GCNGC 2 cut(s) 435, 462
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 2 cut(s) 435, 462
GlaI GCGC 1 cut(s) 305
GluI GCNGC 2 cut(s) 435, 462
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 1 cut(s) 36
HapII CCGG 2 cut(s) 33, 165
HhaI GCGC 1 cut(s) 306
Hin1II CATG 1 cut(s) 122
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HinfI GANTC 2 cut(s) 161, 173
HpaII CCGG 2 cut(s) 33, 165
Hpy188I TCNGA 3 cut(s) 154, 178, 234
Hpy188III TCNNGA 4 cut(s) 19, 56, 165, 266
HpyAV CCTTC 4 cut(s) 173, 193, 276, 472
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 1 cut(s) 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 122
HspAI GCGC 1 cut(s) 304
Kpn2I TCCGGA 1 cut(s) 164
KpnI GGTACC 1 cut(s) 78
Kzo9I GATC 4 cut(s) 61, 204, 498, 502
LpnPI CCDG 6 cut(s) 46, 56, 57, 94, 178, 350
Lsp1109I GCAGC 2 cut(s) 421, 448
MaeII ACGT 1 cut(s) 270
MalI GATC 4 cut(s) 63, 206, 500, 504
MboI GATC 4 cut(s) 61, 204, 498, 502
MboII GAAGA 9 cut(s) 56, 71, 167, 205, 208, 326, 461, 464, 487
MflI RGATCY 2 cut(s) 61, 204
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 1 cut(s) 235
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 4 cut(s) 88, 99, 151, 345
MroI TCCGGA 1 cut(s) 164
MroXI GAANNNNTTC 2 cut(s) 63, 97
MspI CCGG 2 cut(s) 33, 165
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 1 cut(s) 434
NdeII GATC 4 cut(s) 61, 204, 498, 502
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 2 cut(s) 76, 206
NspV TTCGAA 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 264
PdmI GAANNNNTTC 2 cut(s) 63, 97
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 436, 463
Ple19I CGATCG 1 cut(s) 501
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 76, 206
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 2 cut(s) 61, 204
PvuI CGATCG 1 cut(s) 501
RsaI GTAC 1 cut(s) 76
RsaNI GTAC 1 cut(s) 75
SatI GCNGC 2 cut(s) 435, 462
Sau3AI GATC 4 cut(s) 61, 204, 498, 502
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 1 cut(s) 337
SetI ASST 9 cut(s) 80, 115, 265, 273, 356, 409, 436, 463, 483
Sfr274I CTCGAG 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 101
SlaI CTCGAG 1 cut(s) 264
SmlI CTYRAG 2 cut(s) 264, 298
SmoI CTYRAG 2 cut(s) 264, 298
Sse9I AATT 1 cut(s) 235
SsiI CCGC 1 cut(s) 490
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 1 cut(s) 273
TaqI TCGA 5 cut(s) 91, 101, 265, 497, 501
TasI AATT 1 cut(s) 235
TfiI GAWTC 1 cut(s) 173
TseI GCWGC 2 cut(s) 434, 461
TspDTI ATGAA 2 cut(s) 138, 186
XapI RAATTY 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 264
XmnI GAANNNNTTC 2 cut(s) 63, 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.