FvH4_7g04030

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000918
Physical Location & Seq
Reverse (-)
4604650 .. 4605650
1001 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04030.t1

Sequence Viewer

Length: 924 bp
ATGGCCCAAGAGAAGAAGGTGGTCATTCAAGTGTCTGATCTTAGAGTCTATGACAACGGCTCAGTCGACCGGACATGGGCAGGACCCCCCGAGTTCACTTTCCTATTGCAGCCGGTTCCATCTCACGAGCAATTCATTGAAGGCGTTGCCACAAGAGACGTGATCATTGACAAACATCCGGCCATCGCGTCCGAATATACCTACCGGAGAGAAAGCCGGAGGATAATAAGGAGACTAAGCTACCAGATCGCACGTGCCGCCAATGCTATTGTTGTCTCCGTCTATCTCAGACAAGCTCCCGAGCACCGCCTCCCAGCTCAGATCGACGACGGCTTCAATGCTCTCCTCTGGCTCAGATCGGTGGCTCAAGCGGAATCGTATGAACCTTGGCTAAACGACCATGCAGACTTGAACCGGGTTTTCCTCATAGGGGATAGTTCAGGAGGAAACATAGTCCATGAAGTGGCTGCACGAGCAGGGCATGCAGATTTAGGTCAGTTCAAGCTATCCGGTGGGATTCTAATCCAACCTGGCTTTGTCCGGGAAACGAGGAGCAAGTCGGAGTTGGAACAGCCACAGTCTTCGTTTTTCAACGTAGATATGGCGGACAAGTTGTTCAGCTTGTCAATTCCGGTAGGATCAAACAAGGACCATCCGATTACATGTCCGATGGGTTCATTGGCTCCACCATTGGATGGCCTGAAGCTGCCTCCGATGCTGGTCTTCGTTGGGGGGGTCGACGTTTTTCAGGACACTGAGATGGAATACTACGAGGCCATGAAGAAGGCGAACCAGGATGTGCAGCTTTTGACTATCCCCGGAGTCGGTCATGCGTTTTATCTCGCGGCGGACATGGACCCACGCATTGCTGTCCCAATTGCAAGTCTAGTTTCTGGGATCGTAGAGTTCGTCAAGACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

308

Amino Acids

34.0

Weight (kDa)

5.51

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 77 - 280 1.6e-44 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 463, 695
AccI GTMKAC 2 cut(s) 66, 738
AccII CGCG 2 cut(s) 188, 845
AciI CCGC 6 cut(s) 258, 307, 371, 605, 845, 848
AclWI GGATC 2 cut(s) 646, 905
AcoI YGGCCR 1 cut(s) 180
AcuI CTGAAG 1 cut(s) 722
AcvI CACGTG 1 cut(s) 254
AfiI CCNNNNNNNGG 5 cut(s) 76, 430, 463, 695, 824
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 6 cut(s) 29, 140, 337, 412, 502, 592
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 529, 792
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 7 cut(s) 240, 296, 317, 505, 621, 706, 805
AluI AGCT 7 cut(s) 240, 296, 317, 505, 621, 706, 805
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 150, 226, 280
AlwI GGATC 2 cut(s) 646, 905
Ama87I CYCGRG 2 cut(s) 89, 299
AoxI GGCC 4 cut(s) 3, 180, 697, 774
ApeKI GCWGC 4 cut(s) 109, 467, 706, 802
AspS9I GGNCC 4 cut(s) 4, 83, 649, 856
AsuC2I CCSGG 3 cut(s) 416, 542, 819
AvaI CYCGRG 2 cut(s) 89, 299
AvaII GGWCC 3 cut(s) 83, 649, 856
BauI CACGAG 2 cut(s) 125, 471
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 2 cut(s) 573, 715
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 4 cut(s) 121, 454, 693, 814
BccI CCATC 6 cut(s) 127, 191, 660, 664, 689, 754
BceAI ACGGC 2 cut(s) 73, 346
BciT130I CCWGG 2 cut(s) 531, 794
BclI TGATCA 1 cut(s) 162
BcnI CCSGG 3 cut(s) 416, 542, 819
BcoDI GTCTC 3 cut(s) 150, 226, 280
BfaI CTAG 1 cut(s) 887
BisI GCNGC 6 cut(s) 110, 258, 468, 707, 803, 846
BlsI GCNGC 6 cut(s) 111, 259, 469, 708, 804, 847
Bme1390I CCNGG 5 cut(s) 416, 531, 542, 794, 819
Bme18I GGWCC 3 cut(s) 83, 649, 856
BmeT110I CYCGRG 2 cut(s) 89, 299
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 4 cut(s) 4, 83, 649, 856
BmiI GGNNCC 4 cut(s) 85, 117, 684, 858
BmrFI CCNGG 5 cut(s) 416, 531, 542, 794, 819
BmsI GCATC 1 cut(s) 705
BpiI GAAGAC 2 cut(s) 573, 715
BpuEI CTTGAG 1 cut(s) 351
BpuMI CCSGG 3 cut(s) 416, 542, 819
BsaAI YACGTR 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 386, 817
BsaWI WCCGGW 4 cut(s) 69, 204, 509, 631
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 430, 463, 695, 824
Bse118I RCCGGY 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 521
BseBI CCWGG 2 cut(s) 531, 794
BseDI CCNNGG 2 cut(s) 386, 817
BseGI GGATG 4 cut(s) 175, 652, 700, 802
BseJI GATNNNNATC 1 cut(s) 521
BseLI CCNNNNNNNGG 5 cut(s) 76, 430, 463, 695, 824
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 5 cut(s) 75, 301, 332, 367, 747
BseRI GAGGAG 2 cut(s) 335, 565
BseXI GCAGC 4 cut(s) 121, 454, 693, 814
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 2 cut(s) 453, 821
Bsh1236I CGCG 2 cut(s) 188, 845
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 4 cut(s) 5, 182, 699, 776
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 2 cut(s) 89, 299
BslFI GGGAC 1 cut(s) 857
BslI CCNNNNNNNGG 5 cut(s) 76, 430, 463, 695, 824
BsmAI GTCTC 3 cut(s) 150, 226, 280
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 857
BsnI GGCC 4 cut(s) 5, 182, 699, 776
BsoBI CYCGRG 2 cut(s) 89, 299
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
BspACI CCGC 6 cut(s) 258, 307, 371, 605, 845, 848
BspANI GGCC 4 cut(s) 5, 182, 699, 776
BspCNI CTCAG 5 cut(s) 74, 300, 331, 366, 748
BspFNI CGCG 2 cut(s) 188, 845
BspLI GGNNCC 4 cut(s) 85, 117, 684, 858
BspPI GGATC 2 cut(s) 646, 905
BsrDI GCAATG 1 cut(s) 864
BsrFI RCCGGY 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 386, 817
BssMI GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
BssSI CACGAG 2 cut(s) 125, 471
BssT1I CCWWGG 1 cut(s) 386
Bst2BI CACGAG 2 cut(s) 125, 471
Bst2UI CCWGG 2 cut(s) 531, 794
Bst4CI ACNGT 1 cut(s) 579
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 7 cut(s) 41, 61, 236, 287, 318, 353, 756
BstF5I GGATG 4 cut(s) 175, 652, 700, 802
BstFNI CGCG 2 cut(s) 188, 845
BstKTI GATC 7 cut(s) 40, 165, 249, 324, 359, 641, 900
BstMAI GTCTC 3 cut(s) 150, 226, 280
BstMBI GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 5 cut(s) 257, 263, 473, 482, 715
BstNI CCWGG 2 cut(s) 531, 794
BstNSI RCATGY 2 cut(s) 485, 666
BstSCI CCNGG 5 cut(s) 414, 529, 540, 792, 817
BstUI CGCG 2 cut(s) 188, 845
BstV1I GCAGC 4 cut(s) 121, 454, 693, 814
BstV2I GAAGAC 2 cut(s) 573, 715
BsuRI GGCC 4 cut(s) 5, 182, 699, 776
BtgZI GCGATG 1 cut(s) 169
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 4 cut(s) 175, 652, 700, 802
BtsIMutI CAGTG 1 cut(s) 753
Cac8I GCNNGC 1 cut(s) 483
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 4, 83, 649, 856
CseI GACGC 1 cut(s) 177
CviAII CATG 8 cut(s) 75, 401, 458, 482, 663, 778, 830, 853
DdeI CTNAG 7 cut(s) 41, 61, 236, 287, 318, 353, 756
DpnI GATC 7 cut(s) 39, 164, 248, 323, 358, 640, 899
DpnII GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
EaeI YGGCCR 1 cut(s) 180
EciI GGCGGA 2 cut(s) 620, 863
Eco130I CCWWGG 1 cut(s) 386
Eco47I GGWCC 3 cut(s) 83, 649, 856
Eco57I CTGAAG 1 cut(s) 722
Eco72I CACGTG 1 cut(s) 254
Eco88I CYCGRG 2 cut(s) 89, 299
EcoO109I RGGNCCY 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 529, 792
EcoT14I CCWWGG 1 cut(s) 386
ErhI CCWWGG 1 cut(s) 386
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 8 cut(s) 78, 404, 461, 485, 666, 781, 833, 856
FaqI GGGAC 1 cut(s) 857
FatI CATG 8 cut(s) 74, 400, 457, 481, 662, 777, 829, 852
FbaI TGATCA 1 cut(s) 162
FblI GTMKAC 2 cut(s) 66, 738
Fnu4HI GCNGC 6 cut(s) 110, 258, 468, 707, 803, 846
FokI GGATG 4 cut(s) 162, 639, 707, 809
Fsp4HI GCNGC 6 cut(s) 110, 258, 468, 707, 803, 846
FspBI CTAG 1 cut(s) 887
GluI GCNGC 6 cut(s) 110, 258, 468, 707, 803, 846
GsaI CCCAGC 1 cut(s) 317
HaeIII GGCC 4 cut(s) 5, 182, 699, 776
HgaI GACGC 1 cut(s) 177
Hin1II CATG 8 cut(s) 78, 404, 461, 485, 666, 781, 833, 856
HincII GTYRAC 2 cut(s) 67, 739
HindII GTYRAC 2 cut(s) 67, 739
HinfI GANTC 4 cut(s) 45, 374, 517, 822
Hpy166II GTNNAC 3 cut(s) 67, 96, 739
Hpy188I TCNGA 9 cut(s) 37, 193, 290, 321, 356, 562, 657, 669, 714
Hpy188III TCNNGA 5 cut(s) 125, 299, 441, 749, 913
Hpy8I GTNNAC 3 cut(s) 67, 96, 739
Hpy99I CGWCG 3 cut(s) 329, 332, 743
HpyAV CCTTC 3 cut(s) 10, 134, 778
HpyCH4III ACNGT 1 cut(s) 579
HpyCH4IV ACGT 4 cut(s) 159, 253, 594, 741
HpyCH4V TGCA 6 cut(s) 109, 404, 470, 485, 802, 881
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 263, 473, 482, 715
HpyF3I CTNAG 7 cut(s) 41, 61, 236, 287, 318, 353, 756
HpySE526I ACGT 4 cut(s) 159, 253, 594, 741
Hsp92II CATG 8 cut(s) 78, 404, 461, 485, 666, 781, 833, 856
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
LmnI GCTCC 3 cut(s) 301, 552, 688
Lsp1109I GCAGC 4 cut(s) 121, 454, 693, 814
LweI GCATC 1 cut(s) 705
MaeI CTAG 1 cut(s) 887
MaeII ACGT 4 cut(s) 159, 253, 594, 741
MalI GATC 7 cut(s) 39, 164, 248, 323, 358, 640, 899
MboI GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
MboII GAAGA 4 cut(s) 25, 573, 715, 793
MfeI CAATTG 1 cut(s) 876
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 3 cut(s) 131, 627, 876
MlyI GAGTC 2 cut(s) 54, 831
MmeI TCCRAC 3 cut(s) 540, 546, 550
MnlI CCTC 8 cut(s) 213, 320, 356, 434, 437, 543, 720, 766
MslI CAYNNNNRTG 2 cut(s) 29, 758
MspR9I CCNGG 5 cut(s) 416, 531, 542, 794, 819
MunI CAATTG 1 cut(s) 876
MvaI CCWGG 2 cut(s) 531, 794
MvnI CGCG 2 cut(s) 188, 845
MwoI GCNNNNNNNGC 5 cut(s) 257, 263, 473, 482, 715
NciI CCSGG 3 cut(s) 416, 542, 819
NdeII GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
NlaIII CATG 8 cut(s) 78, 404, 461, 485, 666, 781, 833, 856
NlaIV GGNNCC 4 cut(s) 85, 117, 684, 858
NspI RCATGY 2 cut(s) 485, 666
PaeI GCATGC 1 cut(s) 485
PciI ACATGT 1 cut(s) 662
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 906
PfeI GAWTC 2 cut(s) 374, 517
PflMI CCANNNNNTGG 2 cut(s) 463, 695
PfoI TCCNGGA 1 cut(s) 540
PkrI GCNGC 6 cut(s) 111, 259, 469, 708, 804, 847
PleI GAGTC 2 cut(s) 53, 830
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 2 cut(s) 53, 830
Ppu21I YACGTR 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 83
PscI ACATGT 1 cut(s) 662
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 2 cut(s) 529, 792
PspCI CACGTG 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 2 cut(s) 529, 792
PspN4I GGNNCC 4 cut(s) 85, 117, 684, 858
PspPI GGNCC 4 cut(s) 4, 83, 649, 856
PspPPI RGGWCCY 1 cut(s) 83
RseI CAYNNNNRTG 2 cut(s) 29, 758
SalI GTCGAC 2 cut(s) 65, 737
SatI GCNGC 6 cut(s) 110, 258, 468, 707, 803, 846
Sau3AI GATC 7 cut(s) 37, 162, 246, 321, 356, 638, 897
Sau96I GGNCC 4 cut(s) 4, 83, 649, 856
SchI GAGTC 2 cut(s) 54, 831
ScrFI CCNGG 5 cut(s) 416, 531, 542, 794, 819
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 705
SinI GGWCC 3 cut(s) 83, 649, 856
SmiMI CAYNNNNRTG 2 cut(s) 29, 758
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
SphI GCATGC 1 cut(s) 485
Sse9I AATT 3 cut(s) 131, 627, 876
SsiI CCGC 6 cut(s) 258, 307, 371, 605, 845, 848
SspMI CTAG 1 cut(s) 887
StyD4I CCNGG 5 cut(s) 414, 529, 540, 792, 817
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 579
TaiI ACGT 4 cut(s) 162, 256, 597, 744
TaqI TCGA 3 cut(s) 66, 324, 738
TaqII GACCGA 1 cut(s) 815
TasI AATT 3 cut(s) 131, 627, 876
TauI GCSGC 2 cut(s) 260, 848
TfiI GAWTC 2 cut(s) 374, 517
TscAI CASTG 1 cut(s) 760
TseI GCWGC 4 cut(s) 109, 467, 706, 802
TspDTI ATGAA 5 cut(s) 124, 396, 474, 666, 794
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 1 cut(s) 760
Van91I CCANNNNNTGG 2 cut(s) 463, 695
VpaK11BI GGWCC 3 cut(s) 83, 649, 856
XceI RCATGY 2 cut(s) 485, 666
XmiI GTMKAC 2 cut(s) 66, 738
XspI CTAG 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.