pycom02g23560

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000918
Physical Location & Seq
Forward (+)
21644171 .. 21645187
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23560.1

Sequence Viewer

Length: 1017 bp
ATGGTCAGTGAAAATAAAAGGCTAGTTGAGGAGGTGTCTAGTTGGCTAAGAGTCTTCGACGATGGCTCGGTCGATCGGGAGTGGACTGGACCTCCTGAGATTCAGTTCATGGTGGAGCCCGTGCCAGCCCACGATGAATTTGTTGACGGCATTTCCACAAAAGATGTTGTTGCCGACAAATCATCCGGCCTTCGCGTCCGAATTTATTTACCGGAGCGAAAGCCGGAGGGTTATGACACCAAGCTACCAATAATCCTCCATTTCCACGGGGGTGGCTTTTGCATAAGTCAAGCTGATTGGTACATGTACTACCACATATACACGAAGCTATCTCGCTCCACGAATGCCATTGTAGTCTCTGTATATCAGAGGCTCGCACCGGAGCACCGTCTCCCGGCTGCCATTGAGGACGGCTATTCCGCTCTCCTTTGGCTCCGGTCATTGGCTCGAGGGGAGCAAAACGAGCCACTTCTAAGCCATGCAGACTATAACCGTGTGTTTCTTATTGGAGATAGCTCCGGAGGAAACATTGTCCACCACGTGGTGGCTCAAGCGGGGACTGCGGATCTGAGTCCCTTAAGACTTGCCGGCGGGATTCCAATCCACCCCGGTTTCGTGCGAGCGGAGCGGAGTAAGTCGGAGTTGGAGCAGCCTCAGTCACCTCTCCTTACCCTAGACATGGTGGACAAGTTCTTAGGGTTAGCCGTTCCAACGGGGTCCACCAAGAACCATCCCATAACTTGTCCGATGGGTCCCGAGGCCCCACCGTTGGATAATCTGAAGCTTCCGCCGTTCCTTCTCTGCGTTGCTTCCATGGACTTGGTCATAGACACGCAAATGGAGTACTTTGAGGCCATGAAGAAGGCCAACAAGGATGTGGAGCTTCTAATCAACGAGGGGGTGACTCATAGCTTTTATCTGAACAAGATTGCTGTCGACACGGACCCGGAAACAGCCGCAGAAACTGAGCGTCTGATTGCAGGGATCAAACAGTTTATCAAGAACCATTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

339

Amino Acids

37.69

Weight (kDa)

5.32

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 75 - 178 1.7e-09 BD-FAE
Abhydrolase_3 PF07859 85 - 306 2e-48 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 541, 544
AccBSI CCGCTC 3 cut(s) 422, 623, 628
AccI GTMKAC 1 cut(s) 936
AccII CGCG 1 cut(s) 195
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 8 cut(s) 420, 554, 563, 591, 623, 628, 788, 957
AclWI GGATC 2 cut(s) 573, 992
AcsI RAATTY 2 cut(s) 137, 201
AcuI CTGAAG 1 cut(s) 800
AcvI CACGTG 1 cut(s) 541
AdeI CACNNNGTG 2 cut(s) 541, 544
AfaI GTAC 3 cut(s) 302, 308, 845
AfiI CCNNNNNNNGG 6 cut(s) 394, 442, 541, 544, 679, 769
AflII CTTAAG 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 303
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 7 cut(s) 244, 293, 328, 516, 784, 883, 912
AluI AGCT 7 cut(s) 244, 293, 328, 516, 784, 883, 912
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 2 cut(s) 361, 395
AlwI GGATC 2 cut(s) 573, 992
AlwNI CAGNNNCTG 1 cut(s) 965
Ama87I CYCGRG 2 cut(s) 447, 755
Aor13HI TCCGGA 1 cut(s) 518
AoxI GGCC 4 cut(s) 187, 759, 852, 864
ApeKI GCWGC 2 cut(s) 398, 649
ApoI RAATTY 2 cut(s) 137, 201
AspS9I GGNCC 5 cut(s) 89, 717, 752, 760, 943
AsuC2I CCSGG 3 cut(s) 395, 609, 947
AsuHPI GGTGA 2 cut(s) 651, 913
AvaI CYCGRG 2 cut(s) 447, 755
AvaII GGWCC 4 cut(s) 89, 717, 752, 943
BanII GRGCYC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 541
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 385, 661
BccI CCATC 3 cut(s) 56, 738, 742
BceAI ACGGC 4 cut(s) 163, 427, 689, 775
BcnI CCSGG 3 cut(s) 395, 609, 947
BcoDI GTCTC 2 cut(s) 361, 395
BfaI CTAG 3 cut(s) 23, 39, 674
BfrI CTTAAG 1 cut(s) 577
BisI GCNGC 3 cut(s) 399, 650, 957
BlsI GCNGC 3 cut(s) 400, 651, 958
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 3 cut(s) 395, 609, 947
Bme18I GGWCC 4 cut(s) 89, 717, 752, 943
BmeT110I CYCGRG 2 cut(s) 447, 755
BmgT120I GGNCC 5 cut(s) 89, 717, 752, 760, 943
BmiI GGNNCC 7 cut(s) 117, 434, 718, 753, 754, 762, 945
BmrFI CCNGG 3 cut(s) 395, 609, 947
BpiI GAAGAC 1 cut(s) 46
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 3 cut(s) 395, 609, 947
BsaAI YACGTR 1 cut(s) 541
BsaBI GATNNNNATC 1 cut(s) 599
BsaJI CCNNGG 4 cut(s) 265, 607, 756, 813
BsaWI WCCGGW 4 cut(s) 211, 379, 435, 518
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 6 cut(s) 394, 442, 541, 544, 679, 769
Bse118I RCCGGY 1 cut(s) 587
Bse1I ACTGG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 599
BseAI TCCGGA 1 cut(s) 518
BseDI CCNNGG 4 cut(s) 265, 607, 756, 813
BseGI GGATG 3 cut(s) 182, 730, 880
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 6 cut(s) 394, 442, 541, 544, 679, 769
BseMII CTCAG 4 cut(s) 87, 560, 668, 957
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 385, 661
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 2 cut(s) 72, 76
BshFI GGCC 4 cut(s) 189, 761, 854, 866
BsiEI CGRYCG 2 cut(s) 72, 76
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 2 cut(s) 447, 755
BslFI GGGAC 3 cut(s) 558, 571, 738
BslI CCNNNNNNNGG 6 cut(s) 394, 442, 541, 544, 679, 769
BsmAI GTCTC 2 cut(s) 361, 395
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 3 cut(s) 558, 571, 738
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 4 cut(s) 189, 761, 854, 866
BsoBI CYCGRG 2 cut(s) 447, 755
Bsp1286I GDGCHC 2 cut(s) 120, 387
Bsp13I TCCGGA 1 cut(s) 518
Bsp143I GATC 3 cut(s) 73, 565, 984
Bsp19I CCATGG 1 cut(s) 813
BspACI CCGC 8 cut(s) 420, 554, 563, 591, 623, 628, 788, 957
BspANI GGCC 4 cut(s) 189, 761, 854, 866
BspCNI CTCAG 4 cut(s) 88, 561, 667, 958
BspEI TCCGGA 1 cut(s) 518
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 7 cut(s) 117, 434, 718, 753, 754, 762, 945
BspPI GGATC 2 cut(s) 573, 992
BspTI CTTAAG 1 cut(s) 577
BsrBI CCGCTC 3 cut(s) 422, 623, 628
BsrFI RCCGGY 1 cut(s) 587
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 587
BssECI CCNNGG 4 cut(s) 265, 607, 756, 813
BssMI GATC 3 cut(s) 73, 565, 984
BssT1I CCWWGG 1 cut(s) 813
Bst4CI ACNGT 4 cut(s) 389, 494, 768, 993
BstAFI CTTAAG 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 126, 375, 589, 621
BstDEI CTNAG 7 cut(s) 47, 96, 473, 569, 654, 694, 966
BstDSI CCRYGG 2 cut(s) 265, 813
BstF5I GGATG 3 cut(s) 182, 730, 880
BstFNI CGCG 1 cut(s) 195
BstKTI GATC 3 cut(s) 76, 568, 987
BstMAI GTCTC 2 cut(s) 361, 395
BstMBI GATC 3 cut(s) 73, 565, 984
BstMCI CGRYCG 2 cut(s) 72, 76
BstMWI GCNNNNNNNGC 3 cut(s) 463, 560, 625
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 393, 607, 945
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 385, 661
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 1 cut(s) 565
BstXI CCANNNNNNTGG 2 cut(s) 272, 820
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 4 cut(s) 189, 761, 854, 866
BtgI CCRYGG 2 cut(s) 265, 813
BtsCI GGATG 3 cut(s) 182, 730, 880
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 4 cut(s) 126, 375, 589, 621
CaiI CAGNNNCTG 1 cut(s) 965
Cfr10I RCCGGY 1 cut(s) 587
Cfr13I GGNCC 5 cut(s) 89, 717, 752, 760, 943
CseI GACGC 2 cut(s) 184, 959
Csp6I GTAC 3 cut(s) 301, 307, 844
CviAII CATG 6 cut(s) 109, 304, 479, 679, 814, 856
CviQI GTAC 3 cut(s) 301, 307, 844
DdeI CTNAG 7 cut(s) 47, 96, 473, 569, 654, 694, 966
DpnI GATC 3 cut(s) 75, 567, 986
DpnII GATC 3 cut(s) 73, 565, 984
DraIII CACNNNGTG 2 cut(s) 541, 544
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 813
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 4 cut(s) 89, 717, 752, 943
Eco57I CTGAAG 1 cut(s) 800
Eco72I CACGTG 1 cut(s) 541
Eco88I CYCGRG 2 cut(s) 447, 755
EcoO109I RGGNCCY 2 cut(s) 752, 760
EcoT14I CCWWGG 1 cut(s) 813
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 813
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 6 cut(s) 112, 307, 482, 682, 817, 859
FaqI GGGAC 3 cut(s) 558, 571, 738
FatI CATG 6 cut(s) 108, 303, 478, 678, 813, 855
FauI CCCGC 2 cut(s) 547, 584
FblI GTMKAC 1 cut(s) 936
Fnu4HI GCNGC 3 cut(s) 399, 650, 957
FokI GGATG 3 cut(s) 169, 717, 887
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 3 cut(s) 399, 650, 957
FspBI CTAG 3 cut(s) 23, 39, 674
GluI GCNGC 3 cut(s) 399, 650, 957
HaeIII GGCC 4 cut(s) 189, 761, 854, 866
HgaI GACGC 2 cut(s) 184, 959
Hin1II CATG 6 cut(s) 112, 307, 482, 682, 817, 859
HincII GTYRAC 2 cut(s) 145, 937
HindII GTYRAC 2 cut(s) 145, 937
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 5 cut(s) 51, 100, 571, 595, 904
HphI GGTGA 2 cut(s) 651, 913
Hpy166II GTNNAC 6 cut(s) 84, 145, 535, 685, 720, 937
Hpy188I TCNGA 8 cut(s) 200, 369, 570, 640, 747, 780, 921, 975
Hpy188III TCNNGA 5 cut(s) 77, 95, 519, 755, 1000
Hpy8I GTNNAC 6 cut(s) 84, 145, 535, 685, 720, 937
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 200, 806, 856
HpyCH4III ACNGT 4 cut(s) 389, 494, 768, 993
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 3 cut(s) 282, 482, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 463, 560, 625
HpyF3I CTNAG 7 cut(s) 47, 96, 473, 569, 654, 694, 966
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 6 cut(s) 112, 307, 482, 682, 817, 859
KflI GGGWCCC 1 cut(s) 752
Kpn2I TCCGGA 1 cut(s) 518
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Kzo9I GATC 3 cut(s) 73, 565, 984
Lsp1109I GCAGC 2 cut(s) 385, 661
MaeI CTAG 3 cut(s) 23, 39, 674
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 657, 901
MalI GATC 3 cut(s) 75, 567, 986
MbiI CCGCTC 3 cut(s) 422, 623, 628
MboI GATC 3 cut(s) 73, 565, 984
MboII GAAGA 2 cut(s) 46, 871
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 2 cut(s) 120, 387
MluCI AATT 2 cut(s) 137, 201
MlyI GAGTC 3 cut(s) 60, 580, 898
MmeI TCCRAC 4 cut(s) 618, 624, 734, 750
MroI TCCGGA 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 587
MseI TTAA 1 cut(s) 578
MslI CAYNNNNRTG 2 cut(s) 270, 836
MspCI CTTAAG 1 cut(s) 577
MspR9I CCNGG 3 cut(s) 395, 609, 947
Mva1269I GAATGC 1 cut(s) 349
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 3 cut(s) 463, 560, 625
NaeI GCCGGC 1 cut(s) 589
NciI CCSGG 3 cut(s) 395, 609, 947
NcoI CCATGG 1 cut(s) 813
NdeII GATC 3 cut(s) 73, 565, 984
NgoMIV GCCGGC 1 cut(s) 587
NlaIII CATG 6 cut(s) 112, 307, 482, 682, 817, 859
NlaIV GGNNCC 7 cut(s) 117, 434, 718, 753, 754, 762, 945
NmuCI GTSAC 2 cut(s) 657, 901
NspI RCATGY 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 447
PciI ACATGT 1 cut(s) 303
PctI GAATGC 1 cut(s) 349
PdiI GCCGGC 1 cut(s) 589
PfeI GAWTC 2 cut(s) 100, 595
PflFI GACNNNGTC 1 cut(s) 821
PflMI CCANNNNNTGG 2 cut(s) 541, 544
PkrI GCNGC 3 cut(s) 400, 651, 958
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 3 cut(s) 59, 579, 898
PmaCI CACGTG 1 cut(s) 541
PmlI CACGTG 1 cut(s) 541
PpsI GAGTC 3 cut(s) 59, 579, 898
Ppu21I YACGTR 1 cut(s) 541
PpuMI RGGWCCY 1 cut(s) 752
PscI ACATGT 1 cut(s) 303
Psp5II RGGWCCY 1 cut(s) 752
PspCI CACGTG 1 cut(s) 541
PspN4I GGNNCC 7 cut(s) 117, 434, 718, 753, 754, 762, 945
PspPI GGNCC 5 cut(s) 89, 717, 752, 760, 943
PspPPI RGGWCCY 1 cut(s) 752
PspXI VCTCGAGB 1 cut(s) 447
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 565
PsyI GACNNNGTC 1 cut(s) 821
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 3 cut(s) 302, 308, 845
RsaNI GTAC 3 cut(s) 301, 307, 844
RseI CAYNNNNRTG 2 cut(s) 270, 836
SalI GTCGAC 1 cut(s) 935
SaqAI TTAA 1 cut(s) 578
SatI GCNGC 3 cut(s) 399, 650, 957
Sau3AI GATC 3 cut(s) 73, 565, 984
Sau96I GGNCC 5 cut(s) 89, 717, 752, 760, 943
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 3 cut(s) 60, 580, 898
ScrFI CCNGG 3 cut(s) 395, 609, 947
SduI GDGCHC 2 cut(s) 120, 387
Sfr274I CTCGAG 1 cut(s) 447
SinI GGWCC 4 cut(s) 89, 717, 752, 943
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 270, 836
SmlI CTYRAG 3 cut(s) 447, 549, 577
SmoI CTYRAG 3 cut(s) 447, 549, 577
Sse9I AATT 2 cut(s) 137, 201
SsiI CCGC 8 cut(s) 420, 554, 563, 591, 623, 628, 788, 957
SspMI CTAG 3 cut(s) 23, 39, 674
StyD4I CCNGG 3 cut(s) 393, 607, 945
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 4 cut(s) 389, 494, 768, 993
TaiI ACGT 1 cut(s) 543
TaqI TCGA 4 cut(s) 57, 72, 448, 936
TaqII GACCGA 1 cut(s) 58
TasI AATT 2 cut(s) 137, 201
TatI WGTACW 2 cut(s) 306, 843
TauI GCSGC 1 cut(s) 959
TfiI GAWTC 2 cut(s) 100, 595
Tru1I TTAA 1 cut(s) 578
Tru9I TTAA 1 cut(s) 578
TscAI CASTG 1 cut(s) 13
TseFI GTSAC 2 cut(s) 657, 901
TseI GCWGC 2 cut(s) 398, 649
Tsp45I GTSAC 2 cut(s) 657, 901
TspDTI ATGAA 3 cut(s) 97, 150, 872
TspGWI ACGGA 1 cut(s) 956
TspRI CASTG 1 cut(s) 13
Tth111I GACNNNGTC 1 cut(s) 821
Van91I CCANNNNNTGG 2 cut(s) 541, 544
Vha464I CTTAAG 1 cut(s) 577
VpaK11BI GGWCC 4 cut(s) 89, 717, 752, 943
XapI RAATTY 2 cut(s) 137, 201
XceI RCATGY 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 936
XspI CTAG 3 cut(s) 23, 39, 674
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.