Prupe.2G045000_v2.0.a1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000918
Physical Location & Seq
Forward (+)
5037444 .. 5038706
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G045000.1

Sequence Viewer

Length: 1002 bp
ATGGTCTGCCAGAAAGTAGTTGTCAATGAAGTGTCCGGTTGGCTTAGAGTCTTCGACGACGGCTCAGTCGACCGAACATGGACTGGACCTCCTCAGGTCCAGTTCATGACTGAGCCCGTCCCACCCCATGACGAATTCATTGACGGAGTTGCAACCCGTGATGTTTTCGTCAACAAAAACCTCCGCCTACGAATTTATCTGCCGGAAACAAACCCTGAGGACGAGACCAAGCTGCCCATAATACTTCACCTCCATGGAGGAGGCTTTTGCATAAGCCAAGCTGACTGGTACATGTACTACCACATGTACACCAGGCTTGCTCGCTCCGCCAAAGCCATCTGTGTCTCCGTCTACCTAAGGCTGGCGCCCGAGCACCGCCTCCCTGCTCCCGTGAACGACGGCTTCTCTGCTCTTCTCTGGCTCCGTTCCCTCGCTCAAGGAGAGTCATACGAGCCGTGGCTGATCAATCACGGCGACTTTAACCGTGTTTTTCTTATAGGAGACAGCTCAGGCGGTAACCTCGTGCATGAAGTTGCCGCCCGAGCTGGGAAGGCGGATTTGAGTCCGCTAAGGCTAGCGGGCGGGATTCCGATCCACCCGGGTTTTGTTCGGGCAGTGAGGAGCAGGTCGGAGTTGGAGCAGCCAGAGTCGCCTATGCTTACCATTGACATGGTGGACAAGTTTCTGAGCTTGGCATTGCCGGTGGGGTCCACAAAGGACCATCCGATAACTTGTCCAATGGGTTATGGAGCACCGGATTTGGATAGCCTGAAGCTGCCTCCGTTTCTTCTTTGTATTGCAGAGAGGGACATGATTATAGACACAGAGATGGAGTACTATGAGGCCATGAAGAAGGCTAAGAAGGAGGTGGAGCTTTTGATCAGTCCTGGAATGTCTCATAGTTTTTATCTTAACAAGATTGCTGTGGATATGGACCCACAAACAGCTGCCCAAACTGAGGGGCTTATTTCTGGGATCACAGAGTTCGTCAATAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

334

Amino Acids

37.21

Weight (kDa)

5.47

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 615
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 2 cut(s) 69, 351
AciI CCGC 9 cut(s) 184, 327, 376, 513, 537, 554, 566, 578, 582
AclWI GGATC 2 cut(s) 586, 983
AcsI RAATTY 2 cut(s) 134, 192
AcuI CTGAAG 1 cut(s) 791
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 4 cut(s) 290, 296, 308, 836
AfiI CCNNNNNNNGG 3 cut(s) 361, 546, 958
AflIII ACRYGT 2 cut(s) 291, 303
AjnI CCWGG 2 cut(s) 311, 886
AluBI AGCT 8 cut(s) 232, 281, 507, 545, 690, 775, 874, 947
AluI AGCT 8 cut(s) 232, 281, 507, 545, 690, 775, 874, 947
Alw21I GWGCWC 2 cut(s) 375, 754
Alw26I GTCTC 4 cut(s) 218, 349, 495, 900
AlwI GGATC 2 cut(s) 586, 983
Ama87I CYCGRG 3 cut(s) 368, 540, 598
AoxI GGCC 1 cut(s) 843
ApeKI GCWGC 4 cut(s) 232, 640, 775, 947
ApoI RAATTY 2 cut(s) 134, 192
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 5 cut(s) 86, 97, 708, 718, 934
AsuC2I CCSGG 2 cut(s) 599, 600
AsuHPI GGTGA 1 cut(s) 239
AsuNHI GCTAGC 1 cut(s) 574
AvaI CYCGRG 3 cut(s) 368, 540, 598
AvaII GGWCC 5 cut(s) 86, 97, 708, 718, 934
AxyI CCTNAGG 3 cut(s) 93, 216, 356
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 117
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 375, 754
BbvI GCAGC 4 cut(s) 219, 652, 762, 934
BccI CCATC 3 cut(s) 344, 729, 823
BceAI ACGGC 4 cut(s) 76, 415, 439, 487
BciT130I CCWGG 2 cut(s) 313, 888
BclI TGATCA 2 cut(s) 462, 879
BcnI CCSGG 2 cut(s) 599, 600
BcoDI GTCTC 4 cut(s) 218, 349, 495, 900
BfaI CTAG 1 cut(s) 575
BfoI RGCGCY 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 615
BisI GCNGC 5 cut(s) 233, 537, 641, 776, 948
BlsI GCNGC 5 cut(s) 234, 538, 642, 777, 949
BmcAI AGTACT 1 cut(s) 836
Bme1390I CCNGG 4 cut(s) 313, 599, 600, 888
Bme18I GGWCC 5 cut(s) 86, 97, 708, 718, 934
BmeT110I CYCGRG 3 cut(s) 368, 540, 598
BmgT120I GGNCC 5 cut(s) 86, 97, 708, 718, 934
BmiI GGNNCC 4 cut(s) 366, 422, 709, 936
BmrFI CCNGG 4 cut(s) 313, 599, 600, 888
BmtI GCTAGC 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 43
Bpu10I CCTNAGC 2 cut(s) 508, 569
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 2 cut(s) 599, 600
BsaBI GATNNNNATC 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 365
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 3 cut(s) 253, 455, 598
BsaWI WCCGGW 2 cut(s) 35, 754
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 234, 264
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 546, 958
Bse118I RCCGGY 1 cut(s) 700
Bse1I ACTGG 3 cut(s) 88, 100, 290
Bse21I CCTNAGG 3 cut(s) 93, 216, 356
Bse3DI GCAATG 1 cut(s) 695
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 2 cut(s) 313, 888
BseDI CCNNGG 3 cut(s) 253, 455, 598
BseGI GGATG 1 cut(s) 721
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 3 cut(s) 361, 546, 958
BseMI GCAATG 1 cut(s) 695
BseMII CTCAG 7 cut(s) 78, 102, 107, 207, 522, 677, 948
BseNI ACTGG 3 cut(s) 88, 100, 290
BseRI GAGGAG 3 cut(s) 81, 273, 634
BseXI GCAGC 4 cut(s) 219, 652, 762, 934
BseYI CCCAGC 1 cut(s) 545
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 1 cut(s) 845
BshNI GGYRCC 1 cut(s) 364
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 2 cut(s) 375, 754
BsiHKCI CYCGRG 3 cut(s) 368, 540, 598
BsiSI CCGG 5 cut(s) 36, 203, 599, 701, 755
BslFI GGGAC 2 cut(s) 104, 821
BslI CCNNNNNNNGG 3 cut(s) 361, 546, 958
BsmAI GTCTC 4 cut(s) 218, 349, 495, 900
BsmFI GGGAC 2 cut(s) 104, 821
BsnI GGCC 1 cut(s) 845
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 3 cut(s) 368, 540, 598
Bsp1286I GDGCHC 3 cut(s) 117, 375, 754
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 462, 591, 879, 975
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 9 cut(s) 184, 327, 376, 513, 537, 554, 566, 578, 582
BspANI GGCC 1 cut(s) 845
BspCNI CTCAG 7 cut(s) 77, 103, 106, 208, 521, 678, 949
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 4 cut(s) 366, 422, 709, 936
BspMI ACCTGC 1 cut(s) 615
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 2 cut(s) 586, 983
BspQI GCTCTTC 1 cut(s) 417
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 695
BsrFI RCCGGY 1 cut(s) 700
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 3 cut(s) 88, 100, 290
BssAI RCCGGY 1 cut(s) 700
BssECI CCNNGG 3 cut(s) 253, 455, 598
BssMI GATC 4 cut(s) 462, 591, 879, 975
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 2 cut(s) 313, 888
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 1 cut(s) 417
BstACI GRCGYC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 5 cut(s) 318, 322, 363, 576, 580
BstDSI CCRYGG 2 cut(s) 253, 455
BstEII GGTNACC 1 cut(s) 515
BstF5I GGATG 1 cut(s) 721
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 4 cut(s) 465, 594, 882, 978
BstMAI GTCTC 4 cut(s) 218, 349, 495, 900
BstMBI GATC 4 cut(s) 462, 591, 879, 975
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 4 cut(s) 326, 542, 551, 649
BstNI CCWGG 2 cut(s) 313, 888
BstNSI RCATGY 2 cut(s) 295, 307
BstPI GGTNACC 1 cut(s) 515
BstSCI CCNGG 4 cut(s) 311, 597, 598, 886
BstV1I GCAGC 4 cut(s) 219, 652, 762, 934
BstV2I GAAGAC 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 670
Bsu36I CCTNAGG 3 cut(s) 93, 216, 356
BsuRI GGCC 1 cut(s) 845
BtgI CCRYGG 2 cut(s) 253, 455
BtsCI GGATG 1 cut(s) 721
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 1 cut(s) 621
BveI ACCTGC 1 cut(s) 615
Cac8I GCNNGC 5 cut(s) 318, 322, 363, 576, 580
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 367
Cfr10I RCCGGY 1 cut(s) 700
Cfr13I GGNCC 5 cut(s) 86, 97, 708, 718, 934
Cfr9I CCCGGG 1 cut(s) 598
Csp6I GTAC 4 cut(s) 289, 295, 307, 835
CviQI GTAC 4 cut(s) 289, 295, 307, 835
DinI GGCGCC 1 cut(s) 366
DpnI GATC 4 cut(s) 464, 593, 881, 977
DpnII GATC 4 cut(s) 462, 591, 879, 975
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 3 cut(s) 173, 316, 569
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 5 cut(s) 86, 97, 708, 718, 934
Eco57I CTGAAG 1 cut(s) 791
Eco81I CCTNAGG 3 cut(s) 93, 216, 356
Eco88I CYCGRG 3 cut(s) 368, 540, 598
Eco91I GGTNACC 1 cut(s) 515
EcoO65I GGTNACC 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 311, 886
EcoT14I CCWWGG 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 117
EgeI GGCGCC 1 cut(s) 366
EheI GGCGCC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 253
FaqI GGGAC 2 cut(s) 104, 821
FauI CCCGC 2 cut(s) 571, 575
FbaI TGATCA 2 cut(s) 462, 879
FblI GTMKAC 2 cut(s) 69, 351
Fnu4HI GCNGC 5 cut(s) 233, 537, 641, 776, 948
FokI GGATG 1 cut(s) 708
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 5 cut(s) 233, 537, 641, 776, 948
FspBI CTAG 1 cut(s) 575
GlaI GCGC 1 cut(s) 366
GluI GCNGC 5 cut(s) 233, 537, 641, 776, 948
GsaI CCCAGC 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 1 cut(s) 845
HapII CCGG 5 cut(s) 36, 203, 599, 701, 755
HhaI GCGC 1 cut(s) 367
Hin1I GRCGYC 1 cut(s) 365
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 2 cut(s) 70, 172
HindII GTYRAC 2 cut(s) 70, 172
HinfI GANTC 5 cut(s) 48, 443, 562, 586, 647
HpaII CCGG 5 cut(s) 36, 203, 599, 701, 755
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 7 cut(s) 70, 172, 309, 352, 394, 676, 711
Hpy188I TCNGA 4 cut(s) 591, 631, 687, 726
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 7 cut(s) 70, 172, 309, 352, 394, 676, 711
Hpy99I CGWCG 3 cut(s) 59, 62, 401
HpyAV CCTTC 3 cut(s) 544, 847, 856
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4V TGCA 4 cut(s) 152, 270, 526, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 542, 551, 649
Hsp92I GRCGYC 1 cut(s) 365
HspAI GCGC 1 cut(s) 365
KasI GGCGCC 1 cut(s) 364
Ksp22I TGATCA 2 cut(s) 462, 879
Kzo9I GATC 4 cut(s) 462, 591, 879, 975
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 7 cut(s) 329, 391, 426, 621, 637, 749, 871
Lsp1109I GCAGC 4 cut(s) 219, 652, 762, 934
MaeI CTAG 1 cut(s) 575
MaeIII GTNAC 1 cut(s) 515
MalI GATC 4 cut(s) 464, 593, 881, 977
MboI GATC 4 cut(s) 462, 591, 879, 975
MboII GAAGA 4 cut(s) 43, 404, 779, 862
MhlI GDGCHC 3 cut(s) 117, 375, 754
MluCI AATT 2 cut(s) 134, 192
Mly113I GGCGCC 1 cut(s) 365
MlyI GAGTC 4 cut(s) 57, 452, 571, 656
MmeI TCCRAC 2 cut(s) 609, 615
MseI TTAA 2 cut(s) 480, 912
MslI CAYNNNNRTG 3 cut(s) 252, 668, 827
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 5 cut(s) 36, 203, 599, 701, 755
MspR9I CCNGG 4 cut(s) 313, 599, 600, 888
MvaI CCWGG 2 cut(s) 313, 888
MwoI GCNNNNNNNGC 4 cut(s) 326, 542, 551, 649
NarI GGCGCC 1 cut(s) 365
NciI CCSGG 2 cut(s) 599, 600
NcoI CCATGG 1 cut(s) 253
NdeII GATC 4 cut(s) 462, 591, 879, 975
NheI GCTAGC 1 cut(s) 574
NlaIV GGNNCC 4 cut(s) 366, 422, 709, 936
NspI RCATGY 2 cut(s) 295, 307
PagI TCATGA 1 cut(s) 105
PciI ACATGT 2 cut(s) 291, 303
PciSI GCTCTTC 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PfeI GAWTC 1 cut(s) 586
PfoI TCCNGGA 1 cut(s) 886
PkrI GCNGC 5 cut(s) 234, 538, 642, 777, 949
PleI GAGTC 4 cut(s) 56, 451, 570, 655
PluTI GGCGCC 1 cut(s) 368
PpsI GAGTC 4 cut(s) 56, 451, 570, 655
PscI ACATGT 2 cut(s) 291, 303
Psp6I CCWGG 2 cut(s) 311, 886
PspEI GGTNACC 1 cut(s) 515
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 2 cut(s) 311, 886
PspN4I GGNNCC 4 cut(s) 366, 422, 709, 936
PspPI GGNCC 5 cut(s) 86, 97, 708, 718, 934
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 4 cut(s) 290, 296, 308, 836
RsaNI GTAC 4 cut(s) 289, 295, 307, 835
RseI CAYNNNNRTG 3 cut(s) 252, 668, 827
SalI GTCGAC 1 cut(s) 68
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 2 cut(s) 480, 912
SatI GCNGC 5 cut(s) 233, 537, 641, 776, 948
Sau3AI GATC 4 cut(s) 462, 591, 879, 975
Sau96I GGNCC 5 cut(s) 86, 97, 708, 718, 934
ScaI AGTACT 1 cut(s) 836
SchI GAGTC 4 cut(s) 57, 452, 571, 656
ScrFI CCNGG 4 cut(s) 313, 599, 600, 888
SduI GDGCHC 3 cut(s) 117, 375, 754
SfoI GGCGCC 1 cut(s) 366
SinI GGWCC 5 cut(s) 86, 97, 708, 718, 934
SmaI CCCGGG 1 cut(s) 600
SmiMI CAYNNNNRTG 3 cut(s) 252, 668, 827
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 2 cut(s) 134, 192
SsiI CCGC 9 cut(s) 184, 327, 376, 513, 537, 554, 566, 578, 582
SspDI GGCGCC 1 cut(s) 364
SspMI CTAG 1 cut(s) 575
StyD4I CCNGG 4 cut(s) 311, 597, 598, 886
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 54, 69
TaqII GACCGA 1 cut(s) 87
TasI AATT 2 cut(s) 134, 192
TatI WGTACW 3 cut(s) 294, 306, 834
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 2 cut(s) 480, 912
Tru9I TTAA 2 cut(s) 480, 912
TscAI CASTG 1 cut(s) 621
TseI GCWGC 4 cut(s) 232, 640, 775, 947
TspDTI ATGAA 5 cut(s) 42, 94, 127, 543, 863
TspGWI ACGGA 4 cut(s) 159, 337, 413, 771
TspMI CCCGGG 1 cut(s) 598
TspRI CASTG 1 cut(s) 621
VpaK11BI GGWCC 5 cut(s) 86, 97, 708, 718, 934
XapI RAATTY 2 cut(s) 134, 192
XceI RCATGY 2 cut(s) 295, 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 670
XmaI CCCGGG 1 cut(s) 598
XmiI GTMKAC 2 cut(s) 69, 351
XspI CTAG 1 cut(s) 575
ZrmI AGTACT 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.