Rh1BG070900

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000918
Physical Location & Seq
Reverse (-)
10780482 .. 10782270
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG070900.1

Sequence Viewer

Length: 1068 bp
ATGTATTCCCATTTATCATCATCGATCACCATGGTCCGTGAGAACAAGAAGCTTGTCCAAGAAGTGTCCGGTTGGCTAAGAGTCTACGATGACGGCTCAGTCGACCGGACATGGACAGGACCTCCCGAGGTCAAGTTCATGACCGAACCAGTCCCGCCCCATGACGAATTCATCGATGGTGTGGCCACTAAGGACGTATTGGTCGACCAAACCTCCGGCCTCCGCGTCCGGATCTACCTTCCGGAGCAGCAGCCAAAGCACGAGGAGGACGACAACAAAGTAAGCGAGCAACTAAAGCTCCCCATCATACTTCATTTCCATGGAGGAGGCTTCTGCATCACCCAAGCTGACTCGTACATGTACTACCACATATACACTAAGCTCTCGCGCTCCGCCAACGCCATTGTCGTGTCCGTTTACCAACGCCTGGCACCGGAGCACCGCCTCCCTGCCGCAATCGAGGACGGCTACTCCGCTCTCCTTTGGCTCCGCTCTGTCGCCCTAGCGGAACAAAAAGAGCCATGGCTTCTCAGCTATGCGGATTTCAACAGAGTTTTCCTTACCGGAGATAGCTCCGGAGGGAACATCGTCCACCAAGTGGCGGCTCAAGCGGGAAGTACAGACTTAAGTCCACTGAAGCTCGCCGGCGGGATCCCCATCCACCCAGGGTTCGTTCGGTCGGAGAGGAGCAAGTCGGAGCTGGAGCAGCCTCAGTCGCCGTTTCTTACGCTCGACATGGTGGACAAGTTCTTGGGGCTGGCACTGCCTGTAGGTTCCACCAAGGACCATCCGATTACGTGTCCGATGGGATCCGGCGCTCCACCGTTGGAGAGCCTGAAGCTGCCGCCGTTCCTGCTGTGCATTGCGGAGAAGGACCTGATCATTGACACGGAGATGGAGTACTATGAGGCCATGAAGAAGGCGAACAAGGATGTGGAGATTCTGATGAACAAGGGAATGATGCACAGCTTCTATCTCAACAAGATTGCGGTGGACATGGACCCAGAAACAGCTGCAGAGACTGAGCGTCTAATTGCAGGGATCAAAGGGTTCGTGGAGAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

355

Amino Acids

39.61

Weight (kDa)

5.4

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 96 - 205 8.1e-09 BD-FAE
Abhydrolase_3 PF07859 103 - 325 3.5e-51 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 98, 200
AccB1I GGYRCC 1 cut(s) 430
AccB7I CCANNNNNTGG 2 cut(s) 427, 598
AccBSI CCGCTC 2 cut(s) 476, 492
AccI GTMKAC 3 cut(s) 84, 102, 204
AccII CGCG 2 cut(s) 225, 388
AccIII TCCGGA 3 cut(s) 228, 241, 575
AclWI GGATC 6 cut(s) 239, 646, 659, 804, 817, 1049
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 2 cut(s) 656, 857
AdeI CACNNNGTG 1 cut(s) 598
AfaI GTAC 4 cut(s) 356, 362, 619, 902
AfiI CCNNNNNNNGG 4 cut(s) 427, 433, 598, 601
AflII CTTAAG 1 cut(s) 625
AflIII ACRYGT 2 cut(s) 357, 797
AgsI TTSAA 1 cut(s) 547
AhdI GACNNNNNGTC 1 cut(s) 1026
AjnI CCWGG 2 cut(s) 426, 664
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 1 cut(s) 1013
AlwI GGATC 6 cut(s) 239, 646, 659, 804, 817, 1049
AlwNI CAGNNNCTG 1 cut(s) 1022
Ama87I CYCGRG 1 cut(s) 125
Aor13HI TCCGGA 3 cut(s) 228, 241, 575
AoxI GGCC 3 cut(s) 183, 217, 909
ApeKI GCWGC 5 cut(s) 247, 250, 706, 841, 1013
ApoI RAATTY 1 cut(s) 167
AspLEI GCGC 2 cut(s) 390, 818
AspS9I GGNCC 5 cut(s) 34, 119, 784, 874, 1000
AsuHPI GGTGA 2 cut(s) 19, 331
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 5 cut(s) 34, 119, 784, 874, 1000
BalI TGGCCA 1 cut(s) 185
BamHI GGATCC 2 cut(s) 651, 809
BanI GGYRCC 1 cut(s) 430
BauI CACGAG 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 5 cut(s) 259, 262, 718, 828, 1000
BccI CCATC 6 cut(s) 170, 311, 665, 795, 799, 889
BceAI ACGGC 4 cut(s) 109, 481, 703, 832
BciT130I CCWGG 2 cut(s) 428, 666
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 1 cut(s) 1013
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 2 cut(s) 768, 1014
BfoI RGCGCY 1 cut(s) 819
BfrI CTTAAG 1 cut(s) 625
BisI GCNGC 8 cut(s) 248, 251, 453, 603, 707, 842, 845, 1014
BlsI GCNGC 8 cut(s) 249, 252, 454, 604, 708, 843, 846, 1015
BmcAI AGTACT 1 cut(s) 902
Bme1390I CCNGG 2 cut(s) 428, 666
Bme18I GGWCC 5 cut(s) 34, 119, 784, 874, 1000
BmeRI GACNNNNNGTC 1 cut(s) 1026
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 5 cut(s) 34, 119, 784, 874, 1000
BmiI GGNNCC 6 cut(s) 432, 488, 653, 775, 811, 1002
BmrFI CCNGG 2 cut(s) 428, 666
BmsI GCATC 2 cut(s) 345, 951
BoxI GACNNNNGTC 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 591
Bsa29I ATCGAT 2 cut(s) 23, 174
BsaAI YACGTR 1 cut(s) 798
BsaBI GATNNNNATC 1 cut(s) 656
BsaJI CCNNGG 7 cut(s) 30, 126, 319, 521, 664, 665, 780
BsaWI WCCGGW 7 cut(s) 68, 105, 228, 241, 433, 563, 575
Bsc4I CCNNNNNNNGG 4 cut(s) 427, 433, 598, 601
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 861
Bse8I GATNNNNATC 1 cut(s) 656
BseAI TCCGGA 3 cut(s) 228, 241, 575
BseBI CCWGG 2 cut(s) 428, 666
BseCI ATCGAT 2 cut(s) 23, 174
BseDI CCNNGG 7 cut(s) 30, 126, 319, 521, 664, 665, 780
BseGI GGATG 3 cut(s) 657, 787, 937
BseJI GATNNNNATC 1 cut(s) 656
BseLI CCNNNNNNNGG 4 cut(s) 427, 433, 598, 601
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 4 cut(s) 111, 544, 725, 1014
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 3 cut(s) 278, 339, 700
BseXI GCAGC 5 cut(s) 259, 262, 718, 828, 1000
Bsh1236I CGCG 2 cut(s) 225, 388
Bsh1285I CGRYCG 2 cut(s) 106, 680
BshFI GGCC 3 cut(s) 185, 219, 911
BshNI GGYRCC 1 cut(s) 430
BshVI ATCGAT 2 cut(s) 23, 174
BsiEI CGRYCG 2 cut(s) 106, 680
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 4 cut(s) 427, 433, 598, 601
BsmAI GTCTC 1 cut(s) 1013
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 3 cut(s) 185, 219, 911
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 441
Bsp13I TCCGGA 3 cut(s) 228, 241, 575
Bsp143I GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
Bsp19I CCATGG 3 cut(s) 30, 319, 521
BspANI GGCC 3 cut(s) 185, 219, 911
BspCNI CTCAG 4 cut(s) 110, 543, 724, 1015
BspDI ATCGAT 2 cut(s) 23, 174
BspEI TCCGGA 3 cut(s) 228, 241, 575
BspFNI CGCG 2 cut(s) 225, 388
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 6 cut(s) 432, 488, 653, 775, 811, 1002
BspMAI CTGCAG 1 cut(s) 1018
BspPI GGATC 6 cut(s) 239, 646, 659, 804, 817, 1049
BspT107I GGYRCC 1 cut(s) 430
BspTI CTTAAG 1 cut(s) 625
BsrBI CCGCTC 2 cut(s) 476, 492
BsrDI GCAATG 1 cut(s) 861
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 7 cut(s) 30, 126, 319, 521, 664, 665, 780
BssMI GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
BssSI CACGAG 1 cut(s) 260
BssT1I CCWWGG 4 cut(s) 30, 319, 521, 780
Bst2BI CACGAG 1 cut(s) 260
Bst2UI CCWGG 2 cut(s) 428, 666
Bst4CI ACNGT 1 cut(s) 825
BstAFI CTTAAG 1 cut(s) 625
BstBAI YACGTR 1 cut(s) 798
BstC8I GCNNGC 4 cut(s) 287, 642, 646, 759
BstDEI CTNAG 7 cut(s) 77, 97, 189, 378, 530, 711, 1023
BstDSI CCRYGG 3 cut(s) 30, 319, 521
BstF5I GGATG 3 cut(s) 657, 787, 937
BstFNI CGCG 2 cut(s) 225, 388
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 2 cut(s) 390, 818
BstKTI GATC 6 cut(s) 27, 234, 654, 812, 882, 1044
BstMAI GTCTC 1 cut(s) 1013
BstMBI GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
BstMCI CGRYCG 2 cut(s) 106, 680
BstMWI GCNNNNNNNGC 7 cut(s) 256, 295, 608, 706, 715, 763, 853
BstNI CCWGG 2 cut(s) 428, 666
BstNSI RCATGY 1 cut(s) 361
BstPAI GACNNNNGTC 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 426, 664
BstSFI CTRYAG 2 cut(s) 768, 1014
BstUI CGCG 2 cut(s) 225, 388
BstV1I GCAGC 5 cut(s) 259, 262, 718, 828, 1000
BstX2I RGATCY 3 cut(s) 231, 651, 809
BstYI RGATCY 3 cut(s) 231, 651, 809
Bsu15I ATCGAT 2 cut(s) 23, 174
BsuRI GGCC 3 cut(s) 185, 219, 911
BsuTUI ATCGAT 2 cut(s) 23, 174
BtgI CCRYGG 3 cut(s) 30, 319, 521
BtsCI GGATG 3 cut(s) 657, 787, 937
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 3 cut(s) 632, 761, 1063
Cac8I GCNNGC 4 cut(s) 287, 642, 646, 759
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 1 cut(s) 138
CfoI GCGC 2 cut(s) 390, 818
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 5 cut(s) 34, 119, 784, 874, 1000
ClaI ATCGAT 2 cut(s) 23, 174
CseI GACGC 2 cut(s) 214, 1016
Csp6I GTAC 4 cut(s) 355, 361, 618, 901
CviQI GTAC 4 cut(s) 355, 361, 618, 901
DdeI CTNAG 7 cut(s) 77, 97, 189, 378, 530, 711, 1023
DpnI GATC 6 cut(s) 26, 233, 653, 811, 881, 1043
DpnII GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
DraIII CACNNNGTG 1 cut(s) 598
DrdI GACNNNNNNGTC 2 cut(s) 98, 200
DriI GACNNNNNGTC 1 cut(s) 1026
DseDI GACNNNNNNGTC 2 cut(s) 98, 200
EaeI YGGCCR 1 cut(s) 183
Eam1105I GACNNNNNGTC 1 cut(s) 1026
EciI GGCGGA 1 cut(s) 382
Eco130I CCWWGG 4 cut(s) 30, 319, 521, 780
Eco47I GGWCC 5 cut(s) 34, 119, 784, 874, 1000
Eco57I CTGAAG 2 cut(s) 656, 857
Eco88I CYCGRG 1 cut(s) 125
EcoO109I RGGNCCY 2 cut(s) 119, 874
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 426, 664
EcoT14I CCWWGG 4 cut(s) 30, 319, 521, 780
ErhI CCWWGG 4 cut(s) 30, 319, 521, 780
FaqI GGGAC 1 cut(s) 137
FauI CCCGC 3 cut(s) 162, 604, 641
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 3 cut(s) 84, 102, 204
Fnu4HI GCNGC 8 cut(s) 248, 251, 453, 603, 707, 842, 845, 1014
FokI GGATG 3 cut(s) 644, 774, 944
Fsp4HI GCNGC 8 cut(s) 248, 251, 453, 603, 707, 842, 845, 1014
FspBI CTAG 1 cut(s) 503
GlaI GCGC 2 cut(s) 389, 817
GluI GCNGC 8 cut(s) 248, 251, 453, 603, 707, 842, 845, 1014
GsuI CTGGAG 1 cut(s) 722
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 3 cut(s) 185, 219, 911
HgaI GACGC 2 cut(s) 214, 1016
HhaI GCGC 2 cut(s) 390, 818
Hin6I GCGC 2 cut(s) 388, 816
HinP1I GCGC 2 cut(s) 388, 816
HincII GTYRAC 2 cut(s) 103, 205
HindII GTYRAC 2 cut(s) 103, 205
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 3 cut(s) 81, 350, 940
HphI GGTGA 2 cut(s) 19, 331
Hpy166II GTNNAC 8 cut(s) 85, 103, 205, 418, 592, 632, 742, 994
Hpy188I TCNGA 5 cut(s) 682, 697, 792, 804, 945
Hpy188III TCNNGA 5 cut(s) 125, 139, 229, 242, 576
Hpy8I GTNNAC 8 cut(s) 85, 103, 205, 418, 592, 632, 742, 994
HpyAV CCTTC 3 cut(s) 248, 865, 913
HpyCH4III ACNGT 1 cut(s) 825
HpyCH4IV ACGT 2 cut(s) 195, 797
HpyCH4V TGCA 5 cut(s) 336, 861, 964, 1016, 1037
HpyF10VI GCNNNNNNNGC 7 cut(s) 256, 295, 608, 706, 715, 763, 853
HpyF3I CTNAG 7 cut(s) 77, 97, 189, 378, 530, 711, 1023
HpySE526I ACGT 2 cut(s) 195, 797
HspAI GCGC 2 cut(s) 388, 816
Kpn2I TCCGGA 3 cut(s) 228, 241, 575
KroI GCCGGC 1 cut(s) 644
KroNI GCCGGC 1 cut(s) 646
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
Lsp1109I GCAGC 5 cut(s) 259, 262, 718, 828, 1000
LweI GCATC 2 cut(s) 345, 951
MaeI CTAG 1 cut(s) 503
MaeII ACGT 2 cut(s) 195, 797
MalI GATC 6 cut(s) 26, 233, 653, 811, 881, 1043
MbiI CCGCTC 2 cut(s) 476, 492
MboI GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
MboII GAAGA 1 cut(s) 928
MflI RGATCY 3 cut(s) 231, 651, 809
MhlI GDGCHC 1 cut(s) 441
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 2 cut(s) 167, 1032
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 2 cut(s) 90, 344
MmeI TCCRAC 3 cut(s) 660, 675, 807
Mox20I TGGCCA 1 cut(s) 185
MreI CGCCGGCG 1 cut(s) 644
MroI TCCGGA 3 cut(s) 228, 241, 575
MroNI GCCGGC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 185
MseI TTAA 1 cut(s) 626
MslI CAYNNNNRTG 3 cut(s) 318, 407, 893
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 1013
MspCI CTTAAG 1 cut(s) 625
MspR9I CCNGG 2 cut(s) 428, 666
MvaI CCWGG 2 cut(s) 428, 666
MvnI CGCG 2 cut(s) 225, 388
MwoI GCNNNNNNNGC 7 cut(s) 256, 295, 608, 706, 715, 763, 853
NaeI GCCGGC 1 cut(s) 646
NcoI CCATGG 3 cut(s) 30, 319, 521
NdeII GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
NgoMIV GCCGGC 1 cut(s) 644
NlaIV GGNNCC 6 cut(s) 432, 488, 653, 775, 811, 1002
NspI RCATGY 1 cut(s) 361
PagI TCATGA 1 cut(s) 138
PasI CCCWGGG 1 cut(s) 665
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 5 cut(s) 99, 171, 201, 267, 405
PdiI GCCGGC 1 cut(s) 646
PfeI GAWTC 1 cut(s) 940
PflMI CCANNNNNTGG 2 cut(s) 427, 598
PkrI GCNGC 8 cut(s) 249, 252, 454, 604, 708, 843, 846, 1015
PleI GAGTC 2 cut(s) 89, 344
PpsI GAGTC 2 cut(s) 89, 344
Ppu21I YACGTR 1 cut(s) 798
PpuMI RGGWCCY 2 cut(s) 119, 874
PscI ACATGT 1 cut(s) 357
PshAI GACNNNNGTC 1 cut(s) 627
Psp5II RGGWCCY 2 cut(s) 119, 874
Psp6I CCWGG 2 cut(s) 426, 664
PspGI CCWGG 2 cut(s) 426, 664
PspN4I GGNNCC 6 cut(s) 432, 488, 653, 775, 811, 1002
PspPI GGNCC 5 cut(s) 34, 119, 784, 874, 1000
PspPPI RGGWCCY 2 cut(s) 119, 874
PstI CTGCAG 1 cut(s) 1018
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 3 cut(s) 231, 651, 809
PvuII CAGCTG 1 cut(s) 1013
RsaI GTAC 4 cut(s) 356, 362, 619, 902
RsaNI GTAC 4 cut(s) 355, 361, 618, 901
RseI CAYNNNNRTG 3 cut(s) 318, 407, 893
SalI GTCGAC 2 cut(s) 101, 203
SaqAI TTAA 1 cut(s) 626
SatI GCNGC 8 cut(s) 248, 251, 453, 603, 707, 842, 845, 1014
Sau3AI GATC 6 cut(s) 24, 231, 651, 809, 879, 1041
Sau96I GGNCC 5 cut(s) 34, 119, 784, 874, 1000
ScaI AGTACT 1 cut(s) 902
SchI GAGTC 2 cut(s) 90, 344
ScrFI CCNGG 2 cut(s) 428, 666
SduI GDGCHC 1 cut(s) 441
SfaNI GCATC 2 cut(s) 345, 951
SfcI CTRYAG 2 cut(s) 768, 1014
SgrAI CRCCGGYG 1 cut(s) 644
SinI GGWCC 5 cut(s) 34, 119, 784, 874, 1000
SmiMI CAYNNNNRTG 3 cut(s) 318, 407, 893
SmlI CTYRAG 2 cut(s) 606, 625
SmoI CTYRAG 2 cut(s) 606, 625
Sse9I AATT 2 cut(s) 167, 1032
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 2 cut(s) 426, 664
StyI CCWWGG 4 cut(s) 30, 319, 521, 780
TaaI ACNGT 1 cut(s) 825
TaiI ACGT 2 cut(s) 198, 800
TaqI TCGA 6 cut(s) 23, 102, 174, 204, 459, 732
TaqII GACCGA 2 cut(s) 158, 666
TasI AATT 2 cut(s) 167, 1032
TatI WGTACW 3 cut(s) 360, 617, 900
TauI GCSGC 3 cut(s) 455, 605, 847
TfiI GAWTC 1 cut(s) 940
Tru1I TTAA 1 cut(s) 626
Tru9I TTAA 1 cut(s) 626
TscAI CASTG 2 cut(s) 639, 768
TseI GCWGC 5 cut(s) 247, 250, 706, 841, 1013
TspDTI ATGAA 5 cut(s) 127, 160, 302, 929, 962
TspGWI ACGGA 3 cut(s) 26, 403, 905
TspRI CASTG 2 cut(s) 639, 768
Van91I CCANNNNNTGG 2 cut(s) 427, 598
Vha464I CTTAAG 1 cut(s) 625
VpaK11BI GGWCC 5 cut(s) 34, 119, 784, 874, 1000
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 361
XmiI GTMKAC 3 cut(s) 84, 102, 204
XspI CTAG 1 cut(s) 503
ZrmI AGTACT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.