MD02G1275900.v1.1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000918
Physical Location & Seq
Forward (+)
33066264 .. 33067274
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1275900.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGTCAGCGAAAAAAAAAGGCCAGTCGAGGAGGTGTCTGGTTGGCTAAGAGTCTTTGATGATGGCTCTGTCGACCGGAGGTGGACTGGACCTCCTGAGGTTCAGTTCATGGTGGAGCCTGTGGAAGCCCACGATGAATTTATCGACGGAGTTTCCACAAAAGATGTGGTTGCCGACAAATCATCTGGCCATCGTGTCCGAATATATTTACCAGAGCGAAAGCCGGAAAATAATGACACCAAGCTACCAATAATCCTCCATTTCCACGGAGGTGGCTTTTGCATAAGTCAAGCTGATTGGTACATGTACTACCAAATATACACTAAACTATCTCGCTCCACGAATGCCATTGTAATCTCTGTCTATCAGAGGCTCGCACCGGAGCACCGTCTCCCGGCTGCAATCGAGGACGGCTATTCCGCTCTCCTTTGGCTCCGGTCATTGGCTCAAGGCGAGCAAAACGAGCCACTCCTCAGCCATGCAGACTATAACCGTGTGTTTCTTATTGGAGATAGCTCCGGAGGGAACATAGTCCACCATGTGGCGGCTCAATCAGGGACTGCGGATTTGAGTCCCTTAAGACTTGCCGGCGCGATTCCAATCCACCCCGGTTTCGTTCGAGCGGAGCGGAGTAAGTCGCAGTTGGAGCAGCCTCAGTCACCTTTCCTTACCCTAGATATGGTGGACAAGTTCTTAGGGTTAGCCCTTCCAGTGGGGTCCACCAAGGACCATCCCATAACTTGTCCGATAGGTCCCGAGGCCCAACCGTTGGGTAATCTGAAGCTTCCGCCGTTCCTTCTCTGCATTGCTTCCAGGGACTTGATCATAGACACGCAAATGGAGTACTTTGAGGCCATGAAGAAGGCCAACAAGGATGTGGAGCTTCTAATCAACGAGGGGGTGACTCATAGCTTTTATCTGAACAAGATTGCTGTCGACACGGACCCGGAAACTGACGCCGAAACTGAGCGTCTGATTGCAGGGATCAAACAGTTTATCAAGAACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

337

Amino Acids

37.54

Weight (kDa)

5.56

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 74 - 185 8.8e-11 BD-FAE
Abhydrolase_3 PF07859 85 - 306 1.1e-49 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 541, 769
AccBSI CCGCTC 3 cut(s) 422, 623, 628
AccI GTMKAC 2 cut(s) 72, 936
AccII CGCG 1 cut(s) 593
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 6 cut(s) 420, 545, 563, 623, 628, 788
AclWI GGATC 1 cut(s) 992
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 1 cut(s) 957
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 3 cut(s) 302, 308, 845
AfiI CCNNNNNNNGG 7 cut(s) 394, 442, 541, 544, 679, 712, 769
AflII CTTAAG 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 303
AjnI CCWGG 1 cut(s) 812
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 6 cut(s) 244, 293, 516, 784, 883, 912
AluI AGCT 6 cut(s) 244, 293, 516, 784, 883, 912
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 395
AlwI GGATC 1 cut(s) 992
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 755
Aor13HI TCCGGA 1 cut(s) 518
AoxI GGCC 5 cut(s) 20, 187, 759, 852, 864
ApeKI GCWGC 2 cut(s) 398, 649
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 6 cut(s) 89, 717, 727, 752, 760, 943
AsuC2I CCSGG 3 cut(s) 395, 609, 947
AsuHPI GGTGA 2 cut(s) 651, 913
AvaI CYCGRG 1 cut(s) 755
AvaII GGWCC 5 cut(s) 89, 717, 727, 752, 943
AxyI CCTNAGG 1 cut(s) 96
BalI TGGCCA 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 473
BbvI GCAGC 2 cut(s) 385, 661
BccI CCATC 3 cut(s) 56, 198, 738
BceAI ACGGC 2 cut(s) 427, 775
BciT130I CCWGG 1 cut(s) 814
BclI TGATCA 1 cut(s) 822
BcnI CCSGG 3 cut(s) 395, 609, 947
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 1 cut(s) 674
BfrI CTTAAG 1 cut(s) 577
BisI GCNGC 3 cut(s) 399, 546, 650
BlsI GCNGC 3 cut(s) 400, 547, 651
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 4 cut(s) 395, 609, 814, 947
Bme18I GGWCC 5 cut(s) 89, 717, 727, 752, 943
BmeT110I CYCGRG 1 cut(s) 755
BmgT120I GGNCC 6 cut(s) 89, 717, 727, 752, 760, 943
BmiI GGNNCC 5 cut(s) 117, 434, 718, 754, 945
BmrFI CCNGG 4 cut(s) 395, 609, 814, 947
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 432
BpuMI CCSGG 3 cut(s) 395, 609, 947
BsaBI GATNNNNATC 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 957
BsaJI CCNNGG 5 cut(s) 265, 607, 723, 756, 813
BsaWI WCCGGW 4 cut(s) 75, 379, 435, 518
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 7 cut(s) 394, 442, 541, 544, 679, 712, 769
Bse118I RCCGGY 1 cut(s) 587
Bse1I ACTGG 3 cut(s) 23, 91, 710
Bse21I CCTNAGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 1 cut(s) 599
BseAI TCCGGA 1 cut(s) 518
BseBI CCWGG 1 cut(s) 814
BseDI CCNNGG 5 cut(s) 265, 607, 723, 756, 813
BseGI GGATG 2 cut(s) 730, 880
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 7 cut(s) 394, 442, 541, 544, 679, 712, 769
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 4 cut(s) 87, 487, 668, 957
BseNI ACTGG 3 cut(s) 23, 91, 710
BseRI GAGGAG 2 cut(s) 44, 461
BseXI GCAGC 2 cut(s) 385, 661
Bsh1236I CGCG 1 cut(s) 593
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 5 cut(s) 22, 189, 761, 854, 866
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 755
BsiSI CCGG 9 cut(s) 76, 224, 380, 395, 436, 519, 588, 609, 947
BslFI GGGAC 4 cut(s) 558, 571, 738, 830
BslI CCNNNNNNNGG 7 cut(s) 394, 442, 541, 544, 679, 712, 769
BsmAI GTCTC 1 cut(s) 395
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 4 cut(s) 558, 571, 738, 830
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 5 cut(s) 22, 189, 761, 854, 866
BsoBI CYCGRG 1 cut(s) 755
Bsp1286I GDGCHC 1 cut(s) 387
Bsp13I TCCGGA 1 cut(s) 518
Bsp143I GATC 2 cut(s) 822, 984
BspACI CCGC 6 cut(s) 420, 545, 563, 623, 628, 788
BspANI GGCC 5 cut(s) 22, 189, 761, 854, 866
BspCNI CTCAG 4 cut(s) 88, 486, 667, 958
BspEI TCCGGA 1 cut(s) 518
BspFNI CGCG 1 cut(s) 593
BspLI GGNNCC 5 cut(s) 117, 434, 718, 754, 945
BspPI GGATC 1 cut(s) 992
BspTI CTTAAG 1 cut(s) 577
BsrBI CCGCTC 3 cut(s) 422, 623, 628
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 587
BsrI ACTGG 3 cut(s) 23, 91, 710
BssAI RCCGGY 1 cut(s) 587
BssECI CCNNGG 5 cut(s) 265, 607, 723, 756, 813
BssMI GATC 2 cut(s) 822, 984
BssNI GRCGYC 1 cut(s) 957
BssT1I CCWWGG 1 cut(s) 723
Bst2UI CCWGG 1 cut(s) 814
Bst4CI ACNGT 4 cut(s) 389, 494, 768, 993
BstACI GRCGYC 1 cut(s) 957
BstAFI CTTAAG 1 cut(s) 577
BstC8I GCNNGC 3 cut(s) 375, 455, 589
BstDEI CTNAG 6 cut(s) 47, 96, 473, 654, 694, 966
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 2 cut(s) 730, 880
BstFNI CGCG 1 cut(s) 593
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 2 cut(s) 825, 987
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 2 cut(s) 822, 984
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 2 cut(s) 463, 646
BstNI CCWGG 1 cut(s) 814
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 4 cut(s) 393, 607, 812, 945
BstUI CGCG 1 cut(s) 593
BstV1I GCAGC 2 cut(s) 385, 661
BstXI CCANNNNNNTGG 1 cut(s) 272
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 5 cut(s) 22, 189, 761, 854, 866
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 2 cut(s) 730, 880
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 3 cut(s) 375, 455, 589
CaiI CAGNNNCTG 1 cut(s) 560
CfoI GCGC 1 cut(s) 593
Cfr10I RCCGGY 1 cut(s) 587
Cfr13I GGNCC 6 cut(s) 89, 717, 727, 752, 760, 943
CseI GACGC 2 cut(s) 959, 965
Csp6I GTAC 3 cut(s) 301, 307, 844
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 5 cut(s) 109, 304, 479, 539, 856
CviQI GTAC 3 cut(s) 301, 307, 844
DdeI CTNAG 6 cut(s) 47, 96, 473, 654, 694, 966
DpnI GATC 2 cut(s) 824, 986
DpnII GATC 2 cut(s) 822, 984
DraIII CACNNNGTG 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 187
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 723
Eco47I GGWCC 5 cut(s) 89, 717, 727, 752, 943
Eco57I CTGAAG 1 cut(s) 800
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 752
EcoRII CCWGG 1 cut(s) 812
EcoT14I CCWWGG 1 cut(s) 723
ErhI CCWWGG 1 cut(s) 723
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 5 cut(s) 112, 307, 482, 542, 859
FaqI GGGAC 4 cut(s) 558, 571, 738, 830
FatI CATG 5 cut(s) 108, 303, 478, 538, 855
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 2 cut(s) 72, 936
Fnu4HI GCNGC 3 cut(s) 399, 546, 650
FokI GGATG 2 cut(s) 717, 887
Fsp4HI GCNGC 3 cut(s) 399, 546, 650
FspBI CTAG 1 cut(s) 674
GlaI GCGC 1 cut(s) 592
GluI GCNGC 3 cut(s) 399, 546, 650
HaeIII GGCC 5 cut(s) 22, 189, 761, 854, 866
HapII CCGG 9 cut(s) 76, 224, 380, 395, 436, 519, 588, 609, 947
HgaI GACGC 2 cut(s) 959, 965
HhaI GCGC 1 cut(s) 593
Hin1I GRCGYC 1 cut(s) 957
Hin1II CATG 5 cut(s) 112, 307, 482, 542, 859
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HincII GTYRAC 2 cut(s) 73, 937
HindII GTYRAC 2 cut(s) 73, 937
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 4 cut(s) 51, 571, 595, 904
HpaII CCGG 9 cut(s) 76, 224, 380, 395, 436, 519, 588, 609, 947
HphI GGTGA 2 cut(s) 651, 913
Hpy166II GTNNAC 6 cut(s) 73, 84, 535, 685, 720, 937
Hpy188I TCNGA 6 cut(s) 200, 369, 747, 780, 921, 975
Hpy188III TCNNGA 4 cut(s) 95, 519, 755, 1000
Hpy8I GTNNAC 6 cut(s) 73, 84, 535, 685, 720, 937
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 716, 806, 856
HpyCH4III ACNGT 4 cut(s) 389, 494, 768, 993
HpyCH4V TGCA 5 cut(s) 282, 401, 482, 804, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 646
HpyF3I CTNAG 6 cut(s) 47, 96, 473, 654, 694, 966
Hsp92I GRCGYC 1 cut(s) 957
Hsp92II CATG 5 cut(s) 112, 307, 482, 542, 859
HspAI GCGC 1 cut(s) 591
Kpn2I TCCGGA 1 cut(s) 518
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 822
Kzo9I GATC 2 cut(s) 822, 984
LmnI GCTCC 8 cut(s) 115, 341, 382, 438, 521, 625, 646, 880
Lsp1109I GCAGC 2 cut(s) 385, 661
MaeI CTAG 1 cut(s) 674
MaeIII GTNAC 2 cut(s) 657, 901
MalI GATC 2 cut(s) 824, 986
MbiI CCGCTC 3 cut(s) 422, 623, 628
MboI GATC 2 cut(s) 822, 984
MboII GAAGA 1 cut(s) 871
MhlI GDGCHC 1 cut(s) 387
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 1 cut(s) 137
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 3 cut(s) 60, 580, 898
MmeI TCCRAC 1 cut(s) 624
Mox20I TGGCCA 1 cut(s) 189
MroI TCCGGA 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 587
MscI TGGCCA 1 cut(s) 189
MseI TTAA 1 cut(s) 578
MslI CAYNNNNRTG 2 cut(s) 270, 836
Msp20I TGGCCA 1 cut(s) 189
MspCI CTTAAG 1 cut(s) 577
MspI CCGG 9 cut(s) 76, 224, 380, 395, 436, 519, 588, 609, 947
MspR9I CCNGG 4 cut(s) 395, 609, 814, 947
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 1 cut(s) 814
MvnI CGCG 1 cut(s) 593
MwoI GCNNNNNNNGC 2 cut(s) 463, 646
NaeI GCCGGC 1 cut(s) 589
NciI CCSGG 3 cut(s) 395, 609, 947
NdeII GATC 2 cut(s) 822, 984
NgoMIV GCCGGC 1 cut(s) 587
NlaIII CATG 5 cut(s) 112, 307, 482, 542, 859
NlaIV GGNNCC 5 cut(s) 117, 434, 718, 754, 945
NmuCI GTSAC 2 cut(s) 657, 901
NspI RCATGY 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 270
PciI ACATGT 1 cut(s) 303
PctI GAATGC 1 cut(s) 349
PdiI GCCGGC 1 cut(s) 589
PfeI GAWTC 1 cut(s) 595
PflMI CCANNNNNTGG 2 cut(s) 541, 769
PkrI GCNGC 3 cut(s) 400, 547, 651
PleI GAGTC 3 cut(s) 59, 579, 898
PpsI GAGTC 3 cut(s) 59, 579, 898
PpuMI RGGWCCY 1 cut(s) 752
PscI ACATGT 1 cut(s) 303
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 1 cut(s) 812
PspGI CCWGG 1 cut(s) 812
PspN4I GGNNCC 5 cut(s) 117, 434, 718, 754, 945
PspPI GGNCC 6 cut(s) 89, 717, 727, 752, 760, 943
PspPPI RGGWCCY 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 560
RsaI GTAC 3 cut(s) 302, 308, 845
RsaNI GTAC 3 cut(s) 301, 307, 844
RseI CAYNNNNRTG 2 cut(s) 270, 836
SalI GTCGAC 2 cut(s) 71, 935
SaqAI TTAA 1 cut(s) 578
SatI GCNGC 3 cut(s) 399, 546, 650
Sau3AI GATC 2 cut(s) 822, 984
Sau96I GGNCC 6 cut(s) 89, 717, 727, 752, 760, 943
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 3 cut(s) 60, 580, 898
ScrFI CCNGG 4 cut(s) 395, 609, 814, 947
SduI GDGCHC 1 cut(s) 387
SinI GGWCC 5 cut(s) 89, 717, 727, 752, 943
SmiMI CAYNNNNRTG 2 cut(s) 270, 836
SmlI CTYRAG 2 cut(s) 447, 577
SmoI CTYRAG 2 cut(s) 447, 577
Sse9I AATT 1 cut(s) 137
SsiI CCGC 6 cut(s) 420, 545, 563, 623, 628, 788
SspMI CTAG 1 cut(s) 674
StyD4I CCNGG 4 cut(s) 393, 607, 812, 945
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 4 cut(s) 389, 494, 768, 993
TaqI TCGA 6 cut(s) 27, 72, 144, 405, 619, 936
TasI AATT 1 cut(s) 137
TatI WGTACW 2 cut(s) 306, 843
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 595
Tru1I TTAA 1 cut(s) 578
Tru9I TTAA 1 cut(s) 578
TscAI CASTG 1 cut(s) 717
TseFI GTSAC 2 cut(s) 657, 901
TseI GCWGC 2 cut(s) 398, 649
Tsp45I GTSAC 2 cut(s) 657, 901
TspDTI ATGAA 3 cut(s) 97, 150, 872
TspGWI ACGGA 3 cut(s) 162, 282, 956
TspRI CASTG 1 cut(s) 717
Van91I CCANNNNNTGG 2 cut(s) 541, 769
Vha464I CTTAAG 1 cut(s) 577
VpaK11BI GGWCC 5 cut(s) 89, 717, 727, 752, 943
XapI RAATTY 1 cut(s) 137
XceI RCATGY 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 2 cut(s) 163, 874
XmiI GTMKAC 2 cut(s) 72, 936
XspI CTAG 1 cut(s) 674
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.