FvH4_7g04040

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000918
Physical Location & Seq
Forward (+)
4628781 .. 4630243
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04040.t1

Sequence Viewer

Length: 1101 bp
ATGCACACTCTCATTCTCAGTCTTCAATCTCATATATACTCCAAAGTTTTAATATCTAAACCTACAATCAAGCGATCAAATAACACCATGGTCCGCGAGAAGAAGCTCGTTGATGAAGTTTCCGGTTGGCTTAGACTCTACAACGACGGCTCAGTAGACCGGACATGGACTGGACCACCTCAGGTCAAGTTCATGGCTGAGCCCGTTCCACCACATGATGAATTCATCAACGGAGTTGCCACCAAAGATGTCATGGTGGATGAAATGTCTGGCCTTCGCGTCCGAATATATGTACAGGAGAGAAAGCCGGAGGACAACGACGAGACTAAGCTTCCGGTAATCCTTCATTTTCATGGAGGCGGCTTTTGCATTAGCCAAGCAGATTGGTTCATGTACTACCACATGTACGCCAGCCTTGCACTAGCAGCCAATGCCATTTGCGTCTCTGTCTATCTACGCCTTGCACCGGAGCACCGTCTCCCGGCTCCGATCAACGACGGCTGCTCCGCTCTCCTTTGGCTCCGTTCCTTGGCTCGAGCAGAGTCATACGAGTCTTGGCTGAGTGACGATGCTGACTTTTCCCGCGTTTTTCTCATAGGAGATAGCTCAGGTGGCAACATAGTCCATGAAGTGGCCGCTCGAGCTGGGAGACTTGATTTGAGTCCCCTGAAGCTAGCCGGAGCGATTCCGATCCACCCTGGTTTCGTTCGAGCGGTGAGGAGCCGGTCGGAGCTGGAACAACCGGAGTCGCCGTTTCTAACCCTAGACATGGTGGACAAGTTCTTGAGCTTGTCCCTGCCGGTGGGTTCAACCAAGGATCATCCGATTACGTGTCCGATGGGTTCTACGGCGCCAGATTTGGATACTCTGAAGCTGCCGCCGTTCCTTCTCTGTGTTGCGGAGAAGGACATGATTGTGGATACGGAGATGGAGTACTTTGAGGCCATGAAGAAGGCGAAGAAGGATGTGGAACTTCTGATCAGCCCTGGAATGAGTCATAGTTTTTATCTTAACAAGATAGCAATAGATATGGATCCGCAGACGGGTGCGCAAACAGAGAGTCTGATTTCTGGGATAGTAGAGTTCGTCAAGAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

367

Amino Acids

40.83

Weight (kDa)

5.75

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 106 - 209 3.4e-09 BD-FAE
Abhydrolase_3 PF07859 114 - 336 3.7e-50 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1050
AccB1I GGYRCC 1 cut(s) 850
AccB7I CCANNNNNTGG 1 cut(s) 631
AccBSI CCGCTC 3 cut(s) 509, 638, 713
AccI GTMKAC 1 cut(s) 156
AccII CGCG 3 cut(s) 96, 279, 585
AciI CCGC 9 cut(s) 94, 360, 507, 583, 636, 713, 878, 899, 1037
AclWI GGATC 4 cut(s) 685, 825, 1028, 1041
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 2 cut(s) 689, 890
AcyI GRCGYC 1 cut(s) 851
AfaI GTAC 4 cut(s) 294, 395, 407, 935
AfiI CCNNNNNNNGG 7 cut(s) 466, 481, 529, 631, 769, 802, 1043
AflIII ACRYGT 2 cut(s) 402, 830
AgsI TTSAA 2 cut(s) 26, 810
AjnI CCWGG 2 cut(s) 697, 985
AluBI AGCT 8 cut(s) 106, 331, 606, 644, 673, 733, 789, 874
AluI AGCT 8 cut(s) 106, 331, 606, 644, 673, 733, 789, 874
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 317, 448, 482, 643
AlwI GGATC 4 cut(s) 685, 825, 1028, 1041
Ama87I CYCGRG 2 cut(s) 534, 639
AoxI GGCC 3 cut(s) 271, 633, 942
ApeKI GCWGC 3 cut(s) 425, 501, 874
ApoI RAATTY 1 cut(s) 221
ArsI GACNNNNNNTTYG 2 cut(s) 1045, 1077
AspLEI GCGC 2 cut(s) 853, 1051
AspS9I GGNCC 2 cut(s) 91, 173
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 727
AsuNHI GCTAGC 1 cut(s) 673
AvaI CYCGRG 2 cut(s) 534, 639
AvaII GGWCC 2 cut(s) 91, 173
AxyI CCTNAGG 1 cut(s) 180
BamHI GGATCC 1 cut(s) 1033
BanI GGYRCC 1 cut(s) 850
BanII GRGCYC 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 3 cut(s) 437, 488, 861
BccI CCATC 2 cut(s) 832, 922
BceAI ACGGC 5 cut(s) 163, 514, 736, 864, 865
BciT130I CCWGG 2 cut(s) 699, 987
BciVI GTATCC 2 cut(s) 856, 913
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 4 cut(s) 317, 448, 482, 643
BfaI CTAG 3 cut(s) 422, 674, 764
BfoI RGCGCY 1 cut(s) 854
BfuI GTATCC 2 cut(s) 856, 913
BisI GCNGC 6 cut(s) 361, 426, 502, 636, 875, 878
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 6 cut(s) 362, 427, 503, 637, 876, 879
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 3 cut(s) 482, 699, 987
Bme18I GGWCC 2 cut(s) 91, 173
BmeT110I CYCGRG 2 cut(s) 534, 639
BmgT120I GGNCC 2 cut(s) 91, 173
BmiI GGNNCC 5 cut(s) 486, 521, 722, 852, 1035
BmrFI CCNGG 3 cut(s) 482, 699, 987
BmsI GCATC 1 cut(s) 559
BmtI GCTAGC 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 607
Bpu1102I GCTNAGC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 805
BpuMI CCSGG 1 cut(s) 482
BsaAI YACGTR 1 cut(s) 831
BsaBI GATNNNNATC 2 cut(s) 689, 1032
BsaHI GRCGYC 1 cut(s) 851
BsaJI CCNNGG 5 cut(s) 87, 528, 697, 813, 985
BsaWI WCCGGW 5 cut(s) 122, 159, 334, 466, 742
BsaXI ACNNNNNCTCC 6 cut(s) 488, 518, 737, 767, 917, 947
Bsc4I CCNNNNNNNGG 7 cut(s) 466, 481, 529, 631, 769, 802, 1043
Bse118I RCCGGY 2 cut(s) 723, 799
Bse1I ACTGG 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 180
Bse8I GATNNNNATC 2 cut(s) 689, 1032
BseBI CCWGG 2 cut(s) 699, 987
BseDI CCNNGG 5 cut(s) 87, 528, 697, 813, 985
BseGI GGATG 3 cut(s) 265, 820, 970
BseJI GATNNNNATC 2 cut(s) 689, 1032
BseLI CCNNNNNNNGG 7 cut(s) 466, 481, 529, 631, 769, 802, 1043
BseMII CTCAG 6 cut(s) 31, 165, 189, 194, 551, 621
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 1 cut(s) 733
BseXI GCAGC 3 cut(s) 437, 488, 861
BseYI CCCAGC 1 cut(s) 644
Bsh1236I CGCG 3 cut(s) 96, 279, 585
Bsh1285I CGRYCG 1 cut(s) 728
BshFI GGCC 3 cut(s) 273, 635, 944
BshNI GGYRCC 1 cut(s) 850
BsiEI CGRYCG 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 2 cut(s) 534, 639
BslFI GGGAC 2 cut(s) 648, 778
BslI CCNNNNNNNGG 7 cut(s) 466, 481, 529, 631, 769, 802, 1043
BsmAI GTCTC 4 cut(s) 317, 448, 482, 643
BsmBI CGTCTC 2 cut(s) 448, 482
BsmFI GGGAC 2 cut(s) 648, 778
BsnI GGCC 3 cut(s) 273, 635, 944
BsoBI CYCGRG 2 cut(s) 534, 639
Bsp1286I GDGCHC 2 cut(s) 204, 474
Bsp1407I TGTACA 1 cut(s) 292
Bsp143I GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
Bsp1720I GCTNAGC 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 9 cut(s) 94, 360, 507, 583, 636, 713, 878, 899, 1037
BspANI GGCC 3 cut(s) 273, 635, 944
BspCNI CTCAG 6 cut(s) 30, 164, 190, 193, 552, 620
BspFNI CGCG 3 cut(s) 96, 279, 585
BspLI GGNNCC 5 cut(s) 486, 521, 722, 852, 1035
BspOI GCTAGC 1 cut(s) 677
BspPI GGATC 4 cut(s) 685, 825, 1028, 1041
BspT107I GGYRCC 1 cut(s) 850
BsrBI CCGCTC 3 cut(s) 509, 638, 713
BsrFI RCCGGY 2 cut(s) 723, 799
BsrGI TGTACA 1 cut(s) 292
BsrI ACTGG 1 cut(s) 175
BssAI RCCGGY 2 cut(s) 723, 799
BssECI CCNNGG 5 cut(s) 87, 528, 697, 813, 985
BssMI GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 3 cut(s) 87, 528, 813
Bst2UI CCWGG 2 cut(s) 699, 987
Bst4CI ACNGT 1 cut(s) 476
BstACI GRCGYC 1 cut(s) 851
BstAPI GCANNNNNTGC 1 cut(s) 431
BstAUI TGTACA 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 831
BstC8I GCNNGC 2 cut(s) 412, 675
BstDEI CTNAG 8 cut(s) 17, 131, 151, 180, 198, 327, 560, 607
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 3 cut(s) 265, 820, 970
BstFNI CGCG 3 cut(s) 96, 279, 585
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 2 cut(s) 853, 1051
BstKTI GATC 6 cut(s) 77, 492, 693, 820, 981, 1036
BstMAI GTCTC 4 cut(s) 317, 448, 482, 643
BstMBI GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
BstMCI CGRYCG 1 cut(s) 728
BstMWI GCNNNNNNNGC 6 cut(s) 366, 416, 425, 431, 612, 641
BstNI CCWGG 2 cut(s) 699, 987
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 3 cut(s) 480, 697, 985
BstUI CGCG 3 cut(s) 96, 279, 585
BstV1I GCAGC 3 cut(s) 437, 488, 861
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 1033
Bsu36I CCTNAGG 1 cut(s) 180
BsuI GTATCC 2 cut(s) 856, 913
BsuRI GGCC 3 cut(s) 273, 635, 944
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 265, 820, 970
Cac8I GCNNGC 2 cut(s) 412, 675
CfoI GCGC 2 cut(s) 853, 1051
Cfr10I RCCGGY 2 cut(s) 723, 799
Cfr13I GGNCC 2 cut(s) 91, 173
CseI GACGC 2 cut(s) 268, 430
Csp6I GTAC 4 cut(s) 293, 394, 406, 934
CviQI GTAC 4 cut(s) 293, 394, 406, 934
DdeI CTNAG 8 cut(s) 17, 131, 151, 180, 198, 327, 560, 607
DinI GGCGCC 1 cut(s) 852
DpnI GATC 6 cut(s) 76, 491, 692, 819, 980, 1035
DpnII GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
EaeI YGGCCR 1 cut(s) 633
Eco130I CCWWGG 3 cut(s) 87, 528, 813
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 91, 173
Eco57I CTGAAG 2 cut(s) 689, 890
Eco81I CCTNAGG 1 cut(s) 180
Eco88I CYCGRG 2 cut(s) 534, 639
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 697, 985
EcoT14I CCWWGG 3 cut(s) 87, 528, 813
EcoT38I GRGCYC 1 cut(s) 204
EgeI GGCGCC 1 cut(s) 852
EheI GGCGCC 1 cut(s) 852
ErhI CCWWGG 3 cut(s) 87, 528, 813
Esp3I CGTCTC 2 cut(s) 448, 482
FaqI GGGAC 2 cut(s) 648, 778
FauI CCCGC 1 cut(s) 590
FbaI TGATCA 1 cut(s) 978
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 6 cut(s) 361, 426, 502, 636, 875, 878
FokI GGATG 3 cut(s) 272, 807, 977
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 6 cut(s) 361, 426, 502, 636, 875, 878
FspBI CTAG 3 cut(s) 422, 674, 764
FspI TGCGCA 1 cut(s) 1050
GlaI GCGC 2 cut(s) 852, 1050
GluI GCNGC 6 cut(s) 361, 426, 502, 636, 875, 878
GsaI CCCAGC 1 cut(s) 648
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 3 cut(s) 273, 635, 944
HgaI GACGC 2 cut(s) 268, 430
HhaI GCGC 2 cut(s) 853, 1051
Hin1I GRCGYC 1 cut(s) 851
Hin6I GCGC 2 cut(s) 851, 1049
HinP1I GCGC 2 cut(s) 851, 1049
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 8 cut(s) 135, 542, 551, 661, 685, 746, 994, 1060
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 2 cut(s) 157, 775
Hpy188I TCNGA 9 cut(s) 284, 489, 690, 730, 825, 837, 870, 978, 1065
Hpy188III TCNNGA 2 cut(s) 784, 1090
Hpy8I GTNNAC 2 cut(s) 157, 775
Hpy99I CGWCG 3 cut(s) 149, 323, 500
HpyAV CCTTC 6 cut(s) 284, 353, 896, 898, 946, 955
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 830
HpyCH4V TGCA 4 cut(s) 4, 369, 419, 464
HpyF10VI GCNNNNNNNGC 6 cut(s) 366, 416, 425, 431, 612, 641
HpyF3I CTNAG 8 cut(s) 17, 131, 151, 180, 198, 327, 560, 607
HpySE526I ACGT 1 cut(s) 830
Hsp92I GRCGYC 1 cut(s) 851
HspAI GCGC 2 cut(s) 851, 1049
KasI GGCGCC 1 cut(s) 850
Ksp22I TGATCA 1 cut(s) 978
Kzo9I GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
LmnI GCTCC 7 cut(s) 469, 490, 509, 525, 680, 720, 730
Lsp1109I GCAGC 3 cut(s) 437, 488, 861
LweI GCATC 1 cut(s) 559
MaeI CTAG 3 cut(s) 422, 674, 764
MaeII ACGT 1 cut(s) 830
MaeIII GTNAC 1 cut(s) 563
MalI GATC 6 cut(s) 76, 491, 692, 819, 980, 1035
MbiI CCGCTC 3 cut(s) 509, 638, 713
MboI GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
MboII GAAGA 4 cut(s) 14, 112, 961, 970
MflI RGATCY 1 cut(s) 1033
MhlI GDGCHC 2 cut(s) 204, 474
MluCI AATT 1 cut(s) 221
Mly113I GGCGCC 1 cut(s) 851
MlyI GAGTC 7 cut(s) 129, 551, 560, 670, 755, 1003, 1069
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 5 cut(s) 189, 304, 350, 711, 934
MseI TTAA 2 cut(s) 50, 1011
MslI CAYNNNNRTG 2 cut(s) 351, 914
MspR9I CCNGG 3 cut(s) 482, 699, 987
MvaI CCWGG 2 cut(s) 699, 987
MvnI CGCG 3 cut(s) 96, 279, 585
MwoI GCNNNNNNNGC 6 cut(s) 366, 416, 425, 431, 612, 641
NarI GGCGCC 1 cut(s) 851
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 1 cut(s) 87
NdeII GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
NheI GCTAGC 1 cut(s) 673
NlaIV GGNNCC 5 cut(s) 486, 521, 722, 852, 1035
NmuCI GTSAC 1 cut(s) 563
NsbI TGCGCA 1 cut(s) 1050
NspI RCATGY 1 cut(s) 406
PaeR7I CTCGAG 2 cut(s) 534, 639
PciI ACATGT 1 cut(s) 402
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 631
PkrI GCNGC 6 cut(s) 362, 427, 503, 637, 876, 879
PleI GAGTC 7 cut(s) 129, 550, 559, 669, 754, 1002, 1068
PluTI GGCGCC 1 cut(s) 854
PpsI GAGTC 7 cut(s) 129, 550, 559, 669, 754, 1002, 1068
Ppu21I YACGTR 1 cut(s) 831
PscI ACATGT 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 697, 985
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 2 cut(s) 697, 985
PspN4I GGNNCC 5 cut(s) 486, 521, 722, 852, 1035
PspPI GGNCC 2 cut(s) 91, 173
PspXI VCTCGAGB 2 cut(s) 534, 639
PsuI RGATCY 1 cut(s) 1033
RsaI GTAC 4 cut(s) 294, 395, 407, 935
RsaNI GTAC 4 cut(s) 293, 394, 406, 934
RseI CAYNNNNRTG 2 cut(s) 351, 914
SaqAI TTAA 2 cut(s) 50, 1011
SatI GCNGC 6 cut(s) 361, 426, 502, 636, 875, 878
Sau3AI GATC 6 cut(s) 74, 489, 690, 817, 978, 1033
Sau96I GGNCC 2 cut(s) 91, 173
ScaI AGTACT 1 cut(s) 935
SchI GAGTC 7 cut(s) 129, 551, 560, 670, 755, 1003, 1069
ScrFI CCNGG 3 cut(s) 482, 699, 987
SduI GDGCHC 2 cut(s) 204, 474
SfaNI GCATC 1 cut(s) 559
SfoI GGCGCC 1 cut(s) 852
Sfr274I CTCGAG 2 cut(s) 534, 639
SinI GGWCC 2 cut(s) 91, 173
SlaI CTCGAG 2 cut(s) 534, 639
SmiMI CAYNNNNRTG 2 cut(s) 351, 914
SmlI CTYRAG 3 cut(s) 534, 639, 784
SmoI CTYRAG 3 cut(s) 534, 639, 784
Sse9I AATT 1 cut(s) 221
SsiI CCGC 9 cut(s) 94, 360, 507, 583, 636, 713, 878, 899, 1037
SspDI GGCGCC 1 cut(s) 850
SspMI CTAG 3 cut(s) 422, 674, 764
StyD4I CCNGG 3 cut(s) 480, 697, 985
StyI CCWWGG 3 cut(s) 87, 528, 813
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 833
TaqI TCGA 3 cut(s) 535, 640, 709
TasI AATT 1 cut(s) 221
TatI WGTACW 3 cut(s) 292, 393, 933
TauI GCSGC 3 cut(s) 363, 638, 880
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 2 cut(s) 50, 1011
Tru9I TTAA 2 cut(s) 50, 1011
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 3 cut(s) 425, 501, 874
Tsp45I GTSAC 1 cut(s) 563
TspGWI ACGGA 3 cut(s) 246, 512, 938
Van91I CCANNNNNTGG 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 91, 173
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 2 cut(s) 534, 639
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 3 cut(s) 422, 674, 764
ZrmI AGTACT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.