RchiOBHm_Chr1g0327821

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000918
Physical Location & Seq
Reverse (-)
16745494 .. 16746767
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55731

Sequence Viewer

Length: 1005 bp
ATGGTCCAAGAAAAGAAGGTGATCGATGAGGTTTCCGGTTGGCTTAGGCTCTTTGACGACGGATCAGTCGACCGGACATGGACGGGACCCCCTGAGGTCAAGTTCATGACAGAGCCAGTCCCACCGCATGTTGAGTTCATCGACGGAGTTGCGACAAAAGACATGGTCATCGATGAAACGTCCGGCCTTCGAGTTCGAATTTACTTATCTGAGAGAAAGCCGGAGGATTATGAGAGTCTGCCCATATTTCTCCACTTCCATGGAGGCGGCTTTTGCATTAGCCAAGCAGATTGGTACATGTACTACCACATGTACACAAACCTCACACGTGCAGTCAACGCTATATGCGTCTCCGTCTATCTACGCCTCGCACCGGAGCACCGTCTCCCGGCTCCGATCGACGATGGCTTCTCTGCTCTCCTCTGGCTCCGGTCCCTGGCTCGAGGTGATTCATATGAACCATGGTTCAACGACCATGCTGACTTTAACCGTGTTTACCTCCTGGGGGATAGCACAGGAGGAAACATAGTCCATGAAGTGGCTGCAAGAGCAGGGAAGCTGGATTTGAGTCCGGCGAAATTATCCGGGGGGATTCTTATCCACCCTGGTTTTGTCCGGTCATCGAGGAGCAGGTCAGAGCTGGAACAGCCGCAGTCACCTATGCTTACTCTAGACATGGTGGACAAGTTCCTGAGCTTTGCACTGCCGGTGGGGTCCACCAAGGACCATCCGGTTACGTGTCCGATGGGTTCCGGCGCACCACCGTTGGAAAGTCTGCAGCTGCCGCCGTTCCTTGTGTGCGTGGCAGAGAGAGACATCATGGTAGACACTGAGATGGAATACTATGAGGCCATGAAGAAGGCTGGTAAGGATGTGGAGCTCCTGATTAGCACTGGGGTGAGTCACAGTTTTTATCTTAACAAGATTGCTGTGGACATGGATCCGCAAACGGGAGTACAAACTGCAATCCTAATTTCAAGAATCAGAGAGTTCGTCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

334

Amino Acids

37.42

Weight (kDa)

5.15

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 84 - 305 3.2e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 621
AccB7I CCANNNNNTGG 1 cut(s) 538
AccI GTMKAC 2 cut(s) 69, 825
AciI CCGC 5 cut(s) 125, 267, 650, 785, 944
AclWI GGATC 3 cut(s) 70, 935, 948
AcsI RAATTY 1 cut(s) 198
AcvI CACGTG 1 cut(s) 329
AfaI GTAC 4 cut(s) 296, 302, 314, 957
AfiI CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
AflIII ACRYGT 4 cut(s) 297, 309, 326, 737
AgsI TTSAA 2 cut(s) 469, 978
AjnI CCWGG 3 cut(s) 435, 501, 604
AleI CACNNNNGTG 1 cut(s) 896
AluBI AGCT 5 cut(s) 559, 640, 696, 781, 880
AluI AGCT 5 cut(s) 559, 640, 696, 781, 880
Alw21I GWGCWC 2 cut(s) 381, 882
Alw26I GTCTC 3 cut(s) 355, 389, 807
AlwI GGATC 3 cut(s) 70, 935, 948
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 2 cut(s) 184, 849
ApeKI GCWGC 3 cut(s) 542, 778, 781
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 758
AspS9I GGNCC 5 cut(s) 4, 86, 432, 714, 724
AsuC2I CCSGG 2 cut(s) 389, 586
AsuHPI GGTGA 4 cut(s) 31, 458, 648, 910
AsuII TTCGAA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 5 cut(s) 4, 86, 432, 714, 724
AxyI CCTNAGG 1 cut(s) 93
BamHI GGATCC 1 cut(s) 940
BanII GRGCYC 1 cut(s) 882
BbrPI CACGTG 1 cut(s) 329
Bbv12I GWGCWC 2 cut(s) 381, 882
BbvI GCAGC 3 cut(s) 529, 768, 790
BccI CCATC 4 cut(s) 398, 735, 739, 829
BceAI ACGGC 1 cut(s) 772
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 3 cut(s) 437, 503, 606
BcnI CCSGG 2 cut(s) 389, 586
BcoDI GTCTC 3 cut(s) 355, 389, 807
BfaI CTAG 1 cut(s) 671
BfmI CTRYAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 621
BisI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
BlsI GCNGC 6 cut(s) 269, 544, 651, 780, 783, 786
Bme1390I CCNGG 5 cut(s) 389, 437, 503, 586, 606
Bme18I GGWCC 5 cut(s) 4, 86, 432, 714, 724
BmeT110I CYCGRG 1 cut(s) 441
BmgT120I GGNCC 5 cut(s) 4, 86, 432, 714, 724
BmiI GGNNCC 8 cut(s) 87, 88, 393, 428, 434, 715, 751, 942
BmrFI CCNGG 5 cut(s) 389, 437, 503, 586, 606
BmrI ACTGGG 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 903
Bpu10I CCTNAGC 2 cut(s) 44, 692
Bpu14I TTCGAA 1 cut(s) 196
BpuMI CCSGG 2 cut(s) 389, 586
Bsa29I ATCGAT 2 cut(s) 24, 171
BsaAI YACGTR 2 cut(s) 329, 738
BsaBI GATNNNNATC 1 cut(s) 596
BsaJI CCNNGG 7 cut(s) 259, 435, 461, 502, 585, 604, 720
BsaWI WCCGGW 6 cut(s) 35, 72, 373, 429, 615, 730
Bsc4I CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 116, 898
Bse21I CCTNAGG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 596
BseBI CCWGG 3 cut(s) 437, 503, 606
BseCI ATCGAT 2 cut(s) 24, 171
BseDI CCNNGG 7 cut(s) 259, 435, 461, 502, 585, 604, 720
BseGI GGATG 2 cut(s) 727, 877
BseJI GATNNNNATC 1 cut(s) 596
BseLI CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
BseMII CTCAG 4 cut(s) 84, 201, 683, 822
BseNI ACTGG 2 cut(s) 116, 898
BseRI GAGGAG 2 cut(s) 410, 640
BseXI GCAGC 3 cut(s) 529, 768, 790
BsgI GTGCAG 1 cut(s) 351
Bsh1285I CGRYCG 2 cut(s) 73, 399
BshFI GGCC 2 cut(s) 186, 851
BshVI ATCGAT 2 cut(s) 24, 171
BsiEI CGRYCG 2 cut(s) 73, 399
BsiHKAI GWGCWC 2 cut(s) 381, 882
BsiHKCI CYCGRG 1 cut(s) 441
BslFI GGGAC 3 cut(s) 99, 104, 418
BslI CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
BsmAI GTCTC 3 cut(s) 355, 389, 807
BsmBI CGTCTC 2 cut(s) 355, 389
BsmFI GGGAC 3 cut(s) 99, 104, 418
BsnI GGCC 2 cut(s) 186, 851
BsoBI CYCGRG 1 cut(s) 441
Bsp119I TTCGAA 1 cut(s) 196
Bsp1286I GDGCHC 2 cut(s) 381, 882
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 4 cut(s) 21, 62, 396, 940
Bsp19I CCATGG 2 cut(s) 259, 461
BspACI CCGC 5 cut(s) 125, 267, 650, 785, 944
BspANI GGCC 2 cut(s) 186, 851
BspCNI CTCAG 4 cut(s) 85, 202, 684, 823
BspDI ATCGAT 2 cut(s) 24, 171
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 8 cut(s) 87, 88, 393, 428, 434, 715, 751, 942
BspMAI CTGCAG 1 cut(s) 780
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 3 cut(s) 70, 935, 948
BspT104I TTCGAA 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 2 cut(s) 116, 898
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 7 cut(s) 259, 435, 461, 502, 585, 604, 720
BssMI GATC 4 cut(s) 21, 62, 396, 940
BssT1I CCWWGG 3 cut(s) 259, 461, 720
Bst2UI CCWGG 3 cut(s) 437, 503, 606
Bst4CI ACNGT 4 cut(s) 383, 491, 765, 908
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 2 cut(s) 329, 738
BstBI TTCGAA 1 cut(s) 196
BstDEI CTNAG 5 cut(s) 44, 93, 210, 692, 831
BstDSI CCRYGG 2 cut(s) 259, 461
BstF5I GGATG 2 cut(s) 727, 877
BstHHI GCGC 1 cut(s) 758
BstKTI GATC 4 cut(s) 24, 65, 399, 943
BstMAI GTCTC 3 cut(s) 355, 389, 807
BstMBI GATC 4 cut(s) 21, 62, 396, 940
BstMCI CGRYCG 2 cut(s) 73, 399
BstMWI GCNNNNNNNGC 5 cut(s) 273, 338, 548, 646, 784
BstNI CCWGG 3 cut(s) 437, 503, 606
BstNSI RCATGY 3 cut(s) 131, 301, 313
BstSCI CCNGG 5 cut(s) 387, 435, 501, 584, 604
BstSFI CTRYAG 1 cut(s) 776
BstV1I GCAGC 3 cut(s) 529, 768, 790
BstX2I RGATCY 1 cut(s) 940
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 940
Bsu15I ATCGAT 2 cut(s) 24, 171
Bsu36I CCTNAGG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 186, 851
BsuTUI ATCGAT 2 cut(s) 24, 171
BtgI CCRYGG 2 cut(s) 259, 461
BtsCI GGATG 2 cut(s) 727, 877
BtsI GCAGTG 1 cut(s) 701
BtsIMutI CAGTG 3 cut(s) 701, 828, 891
BveI ACCTGC 1 cut(s) 621
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 758
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 5 cut(s) 4, 86, 432, 714, 724
ClaI ATCGAT 2 cut(s) 24, 171
CseI GACGC 1 cut(s) 337
Csp6I GTAC 4 cut(s) 295, 301, 313, 956
CviQI GTAC 4 cut(s) 295, 301, 313, 956
DdeI CTNAG 5 cut(s) 44, 93, 210, 692, 831
DpnI GATC 4 cut(s) 23, 64, 398, 942
DpnII GATC 4 cut(s) 21, 62, 396, 940
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 3 cut(s) 259, 461, 720
Eco24I GRGCYC 1 cut(s) 882
Eco47I GGWCC 5 cut(s) 4, 86, 432, 714, 724
Eco53kI GAGCTC 1 cut(s) 880
Eco72I CACGTG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 3 cut(s) 435, 501, 604
EcoT14I CCWWGG 3 cut(s) 259, 461, 720
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 3 cut(s) 259, 461, 720
Esp3I CGTCTC 2 cut(s) 355, 389
FaqI GGGAC 3 cut(s) 99, 104, 418
FauNDI CATATG 1 cut(s) 454
FblI GTMKAC 2 cut(s) 69, 825
Fnu4HI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
FokI GGATG 2 cut(s) 714, 884
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
FspBI CTAG 1 cut(s) 671
GlaI GCGC 1 cut(s) 757
GluI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
HaeIII GGCC 2 cut(s) 186, 851
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 758
Hin6I GCGC 1 cut(s) 756
HinP1I GCGC 1 cut(s) 756
HincII GTYRAC 3 cut(s) 70, 337, 997
HindII GTYRAC 3 cut(s) 70, 337, 997
HinfI GANTC 6 cut(s) 235, 449, 568, 592, 901, 981
HphI GGTGA 4 cut(s) 31, 458, 648, 910
Hpy166II GTNNAC 9 cut(s) 70, 315, 337, 496, 682, 717, 826, 934, 997
Hpy188I TCNGA 5 cut(s) 211, 396, 637, 744, 986
Hpy188III TCNNGA 5 cut(s) 106, 671, 691, 883, 978
Hpy8I GTNNAC 9 cut(s) 70, 315, 337, 496, 682, 717, 826, 934, 997
Hpy99I CGWCG 3 cut(s) 62, 146, 404
HpyAV CCTTC 3 cut(s) 10, 197, 853
HpyCH4III ACNGT 4 cut(s) 383, 491, 765, 908
HpyCH4IV ACGT 3 cut(s) 179, 328, 737
HpyCH4V TGCA 6 cut(s) 276, 332, 545, 701, 778, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 273, 338, 548, 646, 784
HpyF3I CTNAG 5 cut(s) 44, 93, 210, 692, 831
HpySE526I ACGT 3 cut(s) 179, 328, 737
HspAI GCGC 1 cut(s) 756
KflI GGGWCCC 1 cut(s) 86
Kzo9I GATC 4 cut(s) 21, 62, 396, 940
LmnI GCTCC 6 cut(s) 376, 397, 432, 627, 877, 885
Lsp1109I GCAGC 3 cut(s) 529, 768, 790
MaeI CTAG 1 cut(s) 671
MaeII ACGT 3 cut(s) 179, 328, 737
MaeIII GTNAC 3 cut(s) 654, 733, 902
MalI GATC 4 cut(s) 23, 64, 398, 942
MboI GATC 4 cut(s) 21, 62, 396, 940
MboII GAAGA 1 cut(s) 868
MflI RGATCY 1 cut(s) 940
MhlI GDGCHC 2 cut(s) 381, 882
MluCI AATT 4 cut(s) 198, 578, 972, 1000
MlyI GAGTC 3 cut(s) 244, 577, 910
MmeI TCCRAC 1 cut(s) 747
MseI TTAA 3 cut(s) 486, 918, 1003
MslI CAYNNNNRTG 3 cut(s) 258, 833, 896
MspA1I CMGCKG 1 cut(s) 781
MspR9I CCNGG 5 cut(s) 389, 437, 503, 586, 606
MvaI CCWGG 3 cut(s) 437, 503, 606
MwoI GCNNNNNNNGC 5 cut(s) 273, 338, 548, 646, 784
NciI CCSGG 2 cut(s) 389, 586
NcoI CCATGG 2 cut(s) 259, 461
NdeI CATATG 1 cut(s) 454
NdeII GATC 4 cut(s) 21, 62, 396, 940
NlaIV GGNNCC 8 cut(s) 87, 88, 393, 428, 434, 715, 751, 942
NmuCI GTSAC 2 cut(s) 654, 902
NspI RCATGY 3 cut(s) 131, 301, 313
NspV TTCGAA 1 cut(s) 196
OliI CACNNNNGTG 1 cut(s) 896
PaeR7I CTCGAG 1 cut(s) 441
PagI TCATGA 1 cut(s) 105
PciI ACATGT 2 cut(s) 297, 309
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 345
PfeI GAWTC 3 cut(s) 449, 592, 981
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 1 cut(s) 538
PkrI GCNGC 6 cut(s) 269, 544, 651, 780, 783, 786
Ple19I CGATCG 1 cut(s) 399
PleI GAGTC 3 cut(s) 243, 576, 909
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 3 cut(s) 243, 576, 909
Ppu21I YACGTR 2 cut(s) 329, 738
PpuMI RGGWCCY 1 cut(s) 86
PscI ACATGT 2 cut(s) 297, 309
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 3 cut(s) 435, 501, 604
PspCI CACGTG 1 cut(s) 329
PspGI CCWGG 3 cut(s) 435, 501, 604
PspN4I GGNNCC 8 cut(s) 87, 88, 393, 428, 434, 715, 751, 942
PspPI GGNCC 5 cut(s) 4, 86, 432, 714, 724
PspPPI RGGWCCY 1 cut(s) 86
PspXI VCTCGAGB 1 cut(s) 441
PstI CTGCAG 1 cut(s) 780
PsuI RGATCY 1 cut(s) 940
PsyI GACNNNGTC 1 cut(s) 164
PvuI CGATCG 1 cut(s) 399
PvuII CAGCTG 1 cut(s) 781
RsaI GTAC 4 cut(s) 296, 302, 314, 957
RsaNI GTAC 4 cut(s) 295, 301, 313, 956
RseI CAYNNNNRTG 3 cut(s) 258, 833, 896
SacI GAGCTC 1 cut(s) 882
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 3 cut(s) 486, 918, 1003
SatI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
Sau3AI GATC 4 cut(s) 21, 62, 396, 940
Sau96I GGNCC 5 cut(s) 4, 86, 432, 714, 724
SchI GAGTC 3 cut(s) 244, 577, 910
ScrFI CCNGG 5 cut(s) 389, 437, 503, 586, 606
SduI GDGCHC 2 cut(s) 381, 882
SfcI CTRYAG 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 441
SfuI TTCGAA 1 cut(s) 196
SinI GGWCC 5 cut(s) 4, 86, 432, 714, 724
SlaI CTCGAG 1 cut(s) 441
SmiMI CAYNNNNRTG 3 cut(s) 258, 833, 896
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 4 cut(s) 198, 578, 972, 1000
SsiI CCGC 5 cut(s) 125, 267, 650, 785, 944
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 5 cut(s) 387, 435, 501, 584, 604
StyI CCWWGG 3 cut(s) 259, 461, 720
TaaI ACNGT 4 cut(s) 383, 491, 765, 908
TaiI ACGT 3 cut(s) 182, 331, 740
TaqI TCGA 9 cut(s) 24, 69, 141, 171, 190, 196, 399, 442, 623
TasI AATT 4 cut(s) 198, 578, 972, 1000
TatI WGTACW 3 cut(s) 300, 312, 955
TauI GCSGC 3 cut(s) 270, 652, 787
TfiI GAWTC 3 cut(s) 449, 592, 981
Tru1I TTAA 3 cut(s) 486, 918, 1003
Tru9I TTAA 3 cut(s) 486, 918, 1003
TscAI CASTG 3 cut(s) 708, 835, 898
TseFI GTSAC 2 cut(s) 654, 902
TseI GCWGC 3 cut(s) 542, 778, 781
Tsp45I GTSAC 2 cut(s) 654, 902
TspDTI ATGAA 7 cut(s) 94, 127, 189, 441, 471, 549, 869
TspGWI ACGGA 3 cut(s) 75, 159, 343
TspRI CASTG 3 cut(s) 708, 835, 898
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 538
VpaK11BI GGWCC 5 cut(s) 4, 86, 432, 714, 724
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 3 cut(s) 131, 301, 313
XhoI CTCGAG 1 cut(s) 441
XmiI GTMKAC 2 cut(s) 69, 825
XspI CTAG 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.