Prupe.2G045300_v2.0.a1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000918
Physical Location & Seq
Reverse (-)
5077131 .. 5078165
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G045300.1

Sequence Viewer

Length: 1017 bp
ATGGTCCGTGAAAATAAGAAGCTAGTCGAAGAGGTGTCCGGTTGGCTAAGAGTCTTCAACGACGGCTCAGTCGACCGGACATGGACTGGACCTCCTGAGGTCAAGTTCATGTCTGAGCCAGTGGCACCGCACAAAGAATTCGTCAACGGAGTTGCCACTGAAGATGTGATCGTCGATGAACCCTCTGGCCTTCGCGTACGGATATATCTACCTGAGCAAAAGCAGGAGGGTCACGACGACAACAAGCTTCCCATAATCCTCCACTTCCATGGGGGTGGCTTTTGCATAAGCCAAGCTGACTGGTACATGTACTACCACATATACACCAAGCTTGCTCGCGCCACAAACGCCATTGTTGTCTCTGTCTATCAAAGGCTAGCACCCGAGCACCGTCTCCCTGCCGCCATCGACGACGGCTACACCGCTCTCCTCTGGCTTCGGAAGTTGGCTCGGGGTGAGCAAAAAGAGCCGTGGCTTCTGAGCTACGCAGACTTCAGCCGTGTGTTTCTTATTGGAGATAGCTCAGGAGGGAACATAGTGCACCACGTCGCTGCTAGAGCAGGCACGGTGGATTTGAGTCCCTTGAGGCTTGCCGGAGGGATCCCGATCCACCCGGGTTTCGTTCGAGCCGAGAGGAGCAAGTCTGAATTGGAGCAGCCTGAGTCACCGTTTCTTACACTGGACATGGTGGACAAGTTTTTGGGGTTGGCTATTCCAGTGGGGTCCACCAAAGACCACCCGGTAACGTGTCCGATGGGCCCCGGCGCCCCGCTGTTGGATGGCCTGAAGCTGCCGCCGTTCCTTCTTTGCATTGCTTCCAAGGACTTGATCATAGACACAGAAATGGAGTACTTTGAGGCCATGAAGAAGGCCAACAAGGATGTGGAGCTTCTGATTAACAAGGGGATGACTCACAGCTTCTATCTTAACAAGATTGCACTAGACATGGACCCTGAAACAGCTTCCGAAACTGAGATTCTGATTACAAGGATCAAGGAGTTCATCAAGAACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

339

Amino Acids

37.86

Weight (kDa)

5.76

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccB1I GGYRCC 2 cut(s) 124, 764
AccBSI CCGCTC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 72
AccII CGCG 2 cut(s) 195, 339
AciI CCGC 5 cut(s) 128, 402, 423, 770, 794
AclWI GGATC 4 cut(s) 595, 601, 608, 998
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 3 cut(s) 180, 478, 806
AcyI GRCGYC 1 cut(s) 765
AfaI GTAC 4 cut(s) 198, 305, 311, 851
AfiI CCNNNNNNNGG 1 cut(s) 775
AflIII ACRYGT 2 cut(s) 306, 746
AgsI TTSAA 1 cut(s) 58
AjiI CACGTC 1 cut(s) 547
Alw21I GWGCWC 2 cut(s) 390, 543
Alw26I GTCTC 2 cut(s) 364, 398
Alw44I GTGCAC 1 cut(s) 539
AlwI GGATC 4 cut(s) 595, 601, 608, 998
Ama87I CYCGRG 3 cut(s) 383, 450, 613
AoxI GGCC 5 cut(s) 187, 757, 781, 858, 870
ApaI GGGCCC 1 cut(s) 761
ApaLI GTGCAC 1 cut(s) 539
ApeKI GCWGC 3 cut(s) 551, 655, 790
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AspLEI GCGC 2 cut(s) 341, 767
AspS9I GGNCC 6 cut(s) 4, 89, 723, 757, 758, 949
AsuC2I CCSGG 4 cut(s) 614, 615, 740, 762
AsuHPI GGTGA 2 cut(s) 467, 657
AsuNHI GCTAGC 1 cut(s) 376
AvaI CYCGRG 3 cut(s) 383, 450, 613
AvaII GGWCC 4 cut(s) 4, 89, 723, 949
AxyI CCTNAGG 1 cut(s) 96
BaeGI GKGCMC 2 cut(s) 543, 761
BamHI GGATCC 1 cut(s) 600
BanI GGYRCC 2 cut(s) 124, 764
BanII GRGCYC 1 cut(s) 761
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 2 cut(s) 390, 543
BbvI GCAGC 3 cut(s) 538, 667, 777
BccI CCATC 3 cut(s) 413, 748, 773
BceAI ACGGC 5 cut(s) 79, 430, 454, 483, 781
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 4 cut(s) 614, 615, 740, 762
BcoDI GTCTC 2 cut(s) 364, 398
BfaI CTAG 5 cut(s) 23, 377, 555, 941, 1015
BfoI RGCGCY 1 cut(s) 768
BisI GCNGC 5 cut(s) 402, 552, 656, 791, 794
BlsI GCNGC 5 cut(s) 403, 553, 657, 792, 795
BmcAI AGTACT 1 cut(s) 851
Bme1390I CCNGG 4 cut(s) 614, 615, 740, 762
Bme18I GGWCC 4 cut(s) 4, 89, 723, 949
BmeT110I CYCGRG 3 cut(s) 383, 450, 613
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 6 cut(s) 4, 89, 723, 757, 758, 949
BmiI GGNNCC 7 cut(s) 126, 602, 724, 759, 760, 766, 951
BmrFI CCNGG 4 cut(s) 614, 615, 740, 762
BmtI GCTAGC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 46
Bpu10I CCTNAGC 2 cut(s) 213, 523
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 4 cut(s) 614, 615, 740, 762
BsaBI GATNNNNATC 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 765
BsaJI CCNNGG 5 cut(s) 268, 470, 613, 760, 819
BsaWI WCCGGW 2 cut(s) 38, 75
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 1 cut(s) 775
Bse1I ACTGG 5 cut(s) 91, 119, 305, 684, 716
Bse21I CCTNAGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 810
Bse8I GATNNNNATC 1 cut(s) 605
BseDI CCNNGG 5 cut(s) 268, 470, 613, 760, 819
BseGI GGATG 3 cut(s) 784, 886, 912
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 1 cut(s) 775
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 8 cut(s) 81, 87, 105, 204, 470, 537, 651, 963
BseNI ACTGG 5 cut(s) 91, 119, 305, 684, 716
BseRI GAGGAG 2 cut(s) 419, 649
BseSI GKGCMC 2 cut(s) 543, 761
BseXI GCAGC 3 cut(s) 538, 667, 777
Bsh1236I CGCG 2 cut(s) 195, 339
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 5 cut(s) 189, 759, 783, 860, 872
BshNI GGYRCC 2 cut(s) 124, 764
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 390, 543
BsiHKCI CYCGRG 3 cut(s) 383, 450, 613
BsiSI CCGG 6 cut(s) 39, 76, 594, 614, 740, 762
BsiWI CGTACG 1 cut(s) 196
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 775
BsmAI GTCTC 2 cut(s) 364, 398
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 5 cut(s) 189, 759, 783, 860, 872
BsoBI CYCGRG 3 cut(s) 383, 450, 613
Bsp120I GGGCCC 1 cut(s) 757
Bsp1286I GDGCHC 3 cut(s) 390, 543, 761
Bsp143I GATC 5 cut(s) 168, 600, 606, 828, 990
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 5 cut(s) 128, 402, 423, 770, 794
BspANI GGCC 5 cut(s) 189, 759, 783, 860, 872
BspCNI CTCAG 8 cut(s) 80, 88, 106, 205, 471, 536, 652, 964
BspFNI CGCG 2 cut(s) 195, 339
BspLI GGNNCC 7 cut(s) 126, 602, 724, 759, 760, 766, 951
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 4 cut(s) 595, 601, 608, 998
BspT107I GGYRCC 2 cut(s) 124, 764
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 5 cut(s) 91, 119, 305, 684, 716
BssECI CCNNGG 5 cut(s) 268, 470, 613, 760, 819
BssMI GATC 5 cut(s) 168, 600, 606, 828, 990
BssNI GRCGYC 1 cut(s) 765
BssT1I CCWWGG 2 cut(s) 268, 819
Bst4CI ACNGT 3 cut(s) 392, 568, 669
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 765
BstC8I GCNNGC 5 cut(s) 333, 337, 378, 562, 591
BstDEI CTNAG 9 cut(s) 47, 67, 96, 114, 213, 479, 523, 660, 972
BstDSI CCRYGG 2 cut(s) 268, 470
BstF5I GGATG 3 cut(s) 784, 886, 912
BstFNI CGCG 2 cut(s) 195, 339
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 2 cut(s) 341, 767
BstKTI GATC 5 cut(s) 171, 603, 609, 831, 993
BstMAI GTCTC 2 cut(s) 364, 398
BstMBI GATC 5 cut(s) 168, 600, 606, 828, 990
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 3 cut(s) 347, 466, 557
BstNSI RCATGY 1 cut(s) 310
BstSCI CCNGG 4 cut(s) 612, 613, 738, 760
BstSLI GKGCMC 2 cut(s) 543, 761
BstUI CGCG 2 cut(s) 195, 339
BstV1I GCAGC 3 cut(s) 538, 667, 777
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 1 cut(s) 600
BstXI CCANNNNNNTGG 2 cut(s) 269, 275
BstYI RGATCY 1 cut(s) 600
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 5 cut(s) 189, 759, 783, 860, 872
BtgI CCRYGG 2 cut(s) 268, 470
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 784, 886, 912
BtsIMutI CAGTG 4 cut(s) 126, 156, 677, 723
Cac8I GCNNGC 5 cut(s) 333, 337, 378, 562, 591
CfoI GCGC 2 cut(s) 341, 767
Cfr13I GGNCC 6 cut(s) 4, 89, 723, 757, 758, 949
Cfr9I CCCGGG 1 cut(s) 613
Csp6I GTAC 4 cut(s) 197, 304, 310, 850
CviAII CATG 7 cut(s) 81, 109, 269, 307, 685, 862, 946
CviQI GTAC 4 cut(s) 197, 304, 310, 850
DdeI CTNAG 9 cut(s) 47, 67, 96, 114, 213, 479, 523, 660, 972
DinI GGCGCC 1 cut(s) 766
DpnI GATC 5 cut(s) 170, 602, 608, 830, 992
DpnII GATC 5 cut(s) 168, 600, 606, 828, 990
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 268, 819
Eco24I GRGCYC 1 cut(s) 761
Eco47I GGWCC 4 cut(s) 4, 89, 723, 949
Eco57I CTGAAG 3 cut(s) 180, 478, 806
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 3 cut(s) 383, 450, 613
EcoO109I RGGNCCY 1 cut(s) 758
EcoRI GAATTC 1 cut(s) 137
EcoT14I CCWWGG 2 cut(s) 268, 819
EcoT38I GRGCYC 1 cut(s) 761
EgeI GGCGCC 1 cut(s) 766
EheI GGCGCC 1 cut(s) 766
ErhI CCWWGG 2 cut(s) 268, 819
Esp3I CGTCTC 1 cut(s) 398
FaeI CATG 7 cut(s) 84, 112, 272, 310, 688, 865, 949
FaqI GGGAC 1 cut(s) 564
FatI CATG 7 cut(s) 80, 108, 268, 306, 684, 861, 945
FauI CCCGC 1 cut(s) 777
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 402, 552, 656, 791, 794
FokI GGATG 3 cut(s) 791, 893, 919
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 5 cut(s) 402, 552, 656, 791, 794
FspBI CTAG 5 cut(s) 23, 377, 555, 941, 1015
GlaI GCGC 2 cut(s) 340, 766
GluI GCNGC 5 cut(s) 402, 552, 656, 791, 794
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 5 cut(s) 189, 759, 783, 860, 872
HapII CCGG 6 cut(s) 39, 76, 594, 614, 740, 762
HhaI GCGC 2 cut(s) 341, 767
Hin1I GRCGYC 1 cut(s) 765
Hin1II CATG 7 cut(s) 84, 112, 272, 310, 688, 865, 949
Hin6I GCGC 2 cut(s) 339, 765
HinP1I GCGC 2 cut(s) 339, 765
HincII GTYRAC 2 cut(s) 73, 145
HindII GTYRAC 2 cut(s) 73, 145
HindIII AAGCTT 2 cut(s) 245, 329
HinfI GANTC 5 cut(s) 51, 577, 662, 910, 976
HpaII CCGG 6 cut(s) 39, 76, 594, 614, 740, 762
HphI GGTGA 2 cut(s) 467, 657
Hpy166II GTNNAC 5 cut(s) 73, 145, 541, 691, 726
Hpy188I TCNGA 8 cut(s) 115, 441, 480, 646, 753, 894, 967, 981
Hpy188III TCNNGA 5 cut(s) 95, 233, 525, 604, 1006
Hpy8I GTNNAC 5 cut(s) 73, 145, 541, 691, 726
Hpy99I CGWCG 6 cut(s) 65, 176, 239, 413, 416, 551
HpyAV CCTTC 3 cut(s) 200, 812, 862
HpyCH4III ACNGT 3 cut(s) 392, 568, 669
HpyCH4IV ACGT 2 cut(s) 546, 746
HpyCH4V TGCA 4 cut(s) 285, 541, 810, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 466, 557
HpyF3I CTNAG 9 cut(s) 47, 67, 96, 114, 213, 479, 523, 660, 972
HpySE526I ACGT 2 cut(s) 546, 746
Hsp92I GRCGYC 1 cut(s) 765
Hsp92II CATG 7 cut(s) 84, 112, 272, 310, 688, 865, 949
HspAI GCGC 2 cut(s) 339, 765
KasI GGCGCC 1 cut(s) 764
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 5 cut(s) 168, 600, 606, 828, 990
LmnI GCTCC 3 cut(s) 636, 652, 886
Lsp1109I GCAGC 3 cut(s) 538, 667, 777
MaeI CTAG 5 cut(s) 23, 377, 555, 941, 1015
MaeII ACGT 2 cut(s) 546, 746
MaeIII GTNAC 3 cut(s) 230, 663, 742
MalI GATC 5 cut(s) 170, 602, 608, 830, 992
MbiI CCGCTC 1 cut(s) 425
MboI GATC 5 cut(s) 168, 600, 606, 828, 990
MboII GAAGA 4 cut(s) 41, 46, 173, 877
MflI RGATCY 1 cut(s) 600
MhlI GDGCHC 3 cut(s) 390, 543, 761
MluCI AATT 2 cut(s) 137, 647
Mly113I GGCGCC 1 cut(s) 765
MlyI GAGTC 4 cut(s) 60, 586, 671, 904
MmeI TCCRAC 1 cut(s) 756
MseI TTAA 2 cut(s) 897, 927
MslI CAYNNNNRTG 3 cut(s) 267, 273, 842
MspA1I CMGCKG 1 cut(s) 772
MspI CCGG 6 cut(s) 39, 76, 594, 614, 740, 762
MspR9I CCNGG 4 cut(s) 614, 615, 740, 762
MvnI CGCG 2 cut(s) 195, 339
MwoI GCNNNNNNNGC 3 cut(s) 347, 466, 557
NarI GGCGCC 1 cut(s) 765
NciI CCSGG 4 cut(s) 614, 615, 740, 762
NcoI CCATGG 1 cut(s) 268
NdeII GATC 5 cut(s) 168, 600, 606, 828, 990
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 7 cut(s) 84, 112, 272, 310, 688, 865, 949
NlaIV GGNNCC 7 cut(s) 126, 602, 724, 759, 760, 766, 951
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 2 cut(s) 230, 663
NspI RCATGY 1 cut(s) 310
PciI ACATGT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 627
PfeI GAWTC 1 cut(s) 976
Pfl23II CGTACG 1 cut(s) 196
PkrI GCNGC 5 cut(s) 403, 553, 657, 792, 795
PleI GAGTC 4 cut(s) 59, 585, 670, 904
PluTI GGCGCC 1 cut(s) 768
PpsI GAGTC 4 cut(s) 59, 585, 670, 904
PscI ACATGT 1 cut(s) 306
PspLI CGTACG 1 cut(s) 196
PspN4I GGNNCC 7 cut(s) 126, 602, 724, 759, 760, 766, 951
PspOMI GGGCCC 1 cut(s) 757
PspPI GGNCC 6 cut(s) 4, 89, 723, 757, 758, 949
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 4 cut(s) 198, 305, 311, 851
RsaNI GTAC 4 cut(s) 197, 304, 310, 850
RseI CAYNNNNRTG 3 cut(s) 267, 273, 842
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 897, 927
SatI GCNGC 5 cut(s) 402, 552, 656, 791, 794
Sau3AI GATC 5 cut(s) 168, 600, 606, 828, 990
Sau96I GGNCC 6 cut(s) 4, 89, 723, 757, 758, 949
ScaI AGTACT 1 cut(s) 851
SchI GAGTC 4 cut(s) 60, 586, 671, 904
ScrFI CCNGG 4 cut(s) 614, 615, 740, 762
SduI GDGCHC 3 cut(s) 390, 543, 761
SfoI GGCGCC 1 cut(s) 766
SinI GGWCC 4 cut(s) 4, 89, 723, 949
SmaI CCCGGG 1 cut(s) 615
SmiMI CAYNNNNRTG 3 cut(s) 267, 273, 842
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 2 cut(s) 137, 647
SsiI CCGC 5 cut(s) 128, 402, 423, 770, 794
SspDI GGCGCC 1 cut(s) 764
SspMI CTAG 5 cut(s) 23, 377, 555, 941, 1015
StyD4I CCNGG 4 cut(s) 612, 613, 738, 760
StyI CCWWGG 2 cut(s) 268, 819
TaaI ACNGT 3 cut(s) 392, 568, 669
TaiI ACGT 2 cut(s) 549, 749
TaqI TCGA 5 cut(s) 27, 72, 174, 408, 625
TasI AATT 2 cut(s) 137, 647
TatI WGTACW 2 cut(s) 309, 849
TauI GCSGC 2 cut(s) 404, 796
TfiI GAWTC 1 cut(s) 976
Tru1I TTAA 2 cut(s) 897, 927
Tru9I TTAA 2 cut(s) 897, 927
TscAI CASTG 4 cut(s) 126, 163, 684, 723
TseFI GTSAC 2 cut(s) 230, 663
TseI GCWGC 3 cut(s) 551, 655, 790
Tsp45I GTSAC 2 cut(s) 230, 663
TspDTI ATGAA 4 cut(s) 97, 192, 878, 991
TspGWI ACGGA 2 cut(s) 162, 214
TspMI CCCGGG 1 cut(s) 613
TspRI CASTG 4 cut(s) 126, 163, 684, 723
VneI GTGCAC 1 cut(s) 539
VpaK11BI GGWCC 4 cut(s) 4, 89, 723, 949
XapI RAATTY 1 cut(s) 137
XceI RCATGY 1 cut(s) 310
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmaI CCCGGG 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 5 cut(s) 23, 377, 555, 941, 1015
ZrmI AGTACT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.