Rw1G006820

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000918
Physical Location & Seq
Reverse (-)
14013504 .. 14021166
7663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006820.1

Sequence Viewer

Length: 1002 bp
ATGGTCCATGAGAAGAAGCTGGTCTTGAAAGTATCCGATTGGCTTAGAGTCTATGACGACGGCTCTGTCGACCGGACATGGAGAGGACCTCCTCAGATCAGTTACTTATTGGAGCCTGTTCCACCTCACCAGGAATTCATTAACGGAGTTGCCACCAAAGATGTGGTTATTGACAACACGTCCGGCCATCGTGTCCGAATATATCTACCGGAGAGAAAGCCAGATGATAACAACGAGACTAAGCTACCCATAATCCTTCATTTCCATGGAGGGGGCTTTTGCTTTTGCGAAGCGGATTGGTACATGTACTACCATATCTACACCAAAATTGCACGTGCGGCCAACGCCATTGTTGTCTCCCCCTATCTAAGACAAGCACCCGAGCACCGCCTCCCAGCTCAGATGGAGGACGGCTTTTCTGCTCTCCTCTGGCTCCGATCTCTGGCTCAATCTGATTCACATGAACCGTGGCTAAATGACCATGCCGATTTGAACTGCATTTTCCTCATAGGGGATAGTTCAGGAGGAAACATAGTCCATGAAGTGGCTGCTCGAGCAGGGAATGCGGATTTGGGTACGTTCAAGTTATCCGGTGGGATTCTTATCCAACCTGGCTTTGTCCGGGAAACGAGGAGCAAGTCGGAGTTGGAACAGCCCCAGTCTTCGTTTTTTAGCGTAGATATGGCGGACAACTTTGCACTGCCGGTGGGGTCCACCAAGGACCATCCAGTTACGTGTCCGATGGGTTCCGGCGCACCACCGTTGGAAAGTCTGCAGCTGCCGCGGTTCCTTGTGTGCGTGGCAGAGAGAGACATCATGGTGGACACTGAGATGGAGTACTATGAGGCCATGAAGAAGGCCGGTAAGAATGTGGAGCTCCTGATTAGCACTGGGGTGAGTCACAGCTTTTATCTTAACAAGATTGCTGTGGACATGGATCCGCAAACGGGAGCACAAACTGAAATCCTAATTTCAAGAATCAAAGAGTTCGTCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

333

Amino Acids

37.29

Weight (kDa)

5.45

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 76 - 184 5.6e-08 BD-FAE
Abhydrolase_3 PF07859 85 - 304 8.7e-48 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 544
AccI GTMKAC 1 cut(s) 69
AccII CGCG 1 cut(s) 784
AciI CCGC 8 cut(s) 293, 338, 388, 566, 686, 782, 784, 941
AclWI GGATC 2 cut(s) 932, 945
AcoI YGGCCR 2 cut(s) 184, 339
AcsI RAATTY 1 cut(s) 134
AcvI CACGTG 1 cut(s) 335
AfaI GTAC 4 cut(s) 302, 308, 577, 839
AfiI CCNNNNNNNGG 5 cut(s) 271, 442, 511, 544, 947
AflIII ACRYGT 3 cut(s) 177, 303, 734
AgsI TTSAA 4 cut(s) 28, 493, 583, 975
AhdI GACNNNNNGTC 1 cut(s) 178
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 2 cut(s) 129, 610
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AleI CACNNNNGTG 1 cut(s) 893
AluBI AGCT 6 cut(s) 19, 244, 398, 778, 877, 906
AluI AGCT 6 cut(s) 19, 244, 398, 778, 877, 906
Alw21I GWGCWC 3 cut(s) 387, 879, 955
Alw26I GTCTC 3 cut(s) 230, 361, 804
AlwI GGATC 2 cut(s) 932, 945
Ama87I CYCGRG 2 cut(s) 380, 552
AoxI GGCC 4 cut(s) 184, 339, 846, 858
ApeKI GCWGC 3 cut(s) 548, 775, 778
ApoI RAATTY 1 cut(s) 134
ArsI GACNNNNNNTTYG 1 cut(s) 975
AspLEI GCGC 1 cut(s) 755
AspS9I GGNCC 4 cut(s) 4, 86, 711, 721
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 2 cut(s) 119, 907
AvaI CYCGRG 2 cut(s) 380, 552
AvaII GGWCC 4 cut(s) 4, 86, 711, 721
BamHI GGATCC 1 cut(s) 937
BanII GRGCYC 1 cut(s) 879
BbrPI CACGTG 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 3 cut(s) 387, 879, 955
BbvI GCAGC 3 cut(s) 535, 765, 787
BccI CCATC 5 cut(s) 195, 397, 732, 736, 826
BceAI ACGGC 2 cut(s) 76, 427
BciT130I CCWGG 2 cut(s) 131, 612
BciVI GTATCC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 3 cut(s) 230, 361, 804
BfmI CTRYAG 1 cut(s) 773
BfuI GTATCC 1 cut(s) 43
BisI GCNGC 5 cut(s) 339, 549, 776, 779, 782
BlsI GCNGC 5 cut(s) 340, 550, 777, 780, 783
BmcAI AGTACT 1 cut(s) 839
Bme1390I CCNGG 3 cut(s) 131, 612, 623
Bme18I GGWCC 4 cut(s) 4, 86, 711, 721
BmeRI GACNNNNNGTC 1 cut(s) 178
BmeT110I CYCGRG 2 cut(s) 380, 552
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 4 cut(s) 4, 86, 711, 721
BmiI GGNNCC 6 cut(s) 114, 434, 712, 748, 788, 939
BmrFI CCNGG 3 cut(s) 131, 612, 623
BmrI ACTGGG 2 cut(s) 652, 900
BmuI ACTGGG 2 cut(s) 652, 900
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpuMI CCSGG 1 cut(s) 623
BsaAI YACGTR 2 cut(s) 335, 735
BsaBI GATNNNNATC 1 cut(s) 602
BsaJI CCNNGG 4 cut(s) 265, 467, 717, 782
BsaWI WCCGGW 3 cut(s) 72, 208, 590
Bsc4I CCNNNNNNNGG 5 cut(s) 271, 442, 511, 544, 947
Bse118I RCCGGY 2 cut(s) 703, 860
Bse1I ACTGG 3 cut(s) 658, 728, 895
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 2 cut(s) 131, 612
BseDI CCNNGG 4 cut(s) 265, 467, 717, 782
BseGI GGATG 1 cut(s) 724
BseJI GATNNNNATC 1 cut(s) 602
BseLI CCNNNNNNNGG 5 cut(s) 271, 442, 511, 544, 947
BseMII CTCAG 3 cut(s) 107, 413, 819
BseNI ACTGG 3 cut(s) 658, 728, 895
BseRI GAGGAG 3 cut(s) 81, 416, 646
BseXI GCAGC 3 cut(s) 535, 765, 787
BseYI CCCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 784
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 4 cut(s) 186, 341, 848, 860
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 3 cut(s) 387, 879, 955
BsiHKCI CYCGRG 2 cut(s) 380, 552
BsiSI CCGG 8 cut(s) 73, 183, 209, 591, 622, 704, 750, 861
BslI CCNNNNNNNGG 5 cut(s) 271, 442, 511, 544, 947
BsmAI GTCTC 3 cut(s) 230, 361, 804
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 4 cut(s) 186, 341, 848, 860
BsoBI CYCGRG 2 cut(s) 380, 552
Bsp1286I GDGCHC 3 cut(s) 387, 879, 955
Bsp143I GATC 3 cut(s) 96, 437, 937
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 8 cut(s) 293, 338, 388, 566, 686, 782, 784, 941
BspANI GGCC 4 cut(s) 186, 341, 848, 860
BspCNI CTCAG 3 cut(s) 106, 412, 820
BspFNI CGCG 1 cut(s) 784
BspLI GGNNCC 6 cut(s) 114, 434, 712, 748, 788, 939
BspMAI CTGCAG 1 cut(s) 777
BspPI GGATC 2 cut(s) 932, 945
BsrFI RCCGGY 2 cut(s) 703, 860
BsrI ACTGG 3 cut(s) 658, 728, 895
BssAI RCCGGY 2 cut(s) 703, 860
BssECI CCNNGG 4 cut(s) 265, 467, 717, 782
BssMI GATC 3 cut(s) 96, 437, 937
BssT1I CCWWGG 2 cut(s) 265, 717
Bst2UI CCWGG 2 cut(s) 131, 612
Bst4CI ACNGT 2 cut(s) 468, 762
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBAI YACGTR 2 cut(s) 335, 735
BstDEI CTNAG 6 cut(s) 44, 93, 240, 368, 399, 828
BstDSI CCRYGG 3 cut(s) 265, 467, 782
BstF5I GGATG 1 cut(s) 724
BstFNI CGCG 1 cut(s) 784
BstHHI GCGC 1 cut(s) 755
BstKTI GATC 3 cut(s) 99, 440, 940
BstMAI GTCTC 3 cut(s) 230, 361, 804
BstMBI GATC 3 cut(s) 96, 437, 937
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 5 cut(s) 338, 344, 554, 563, 781
BstNI CCWGG 2 cut(s) 131, 612
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 129, 610, 621
BstSFI CTRYAG 1 cut(s) 773
BstUI CGCG 1 cut(s) 784
BstV1I GCAGC 3 cut(s) 535, 765, 787
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 937
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 1 cut(s) 937
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 4 cut(s) 186, 341, 848, 860
BtgI CCRYGG 3 cut(s) 265, 467, 782
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 1 cut(s) 724
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 3 cut(s) 698, 825, 888
CfoI GCGC 1 cut(s) 755
Cfr10I RCCGGY 2 cut(s) 703, 860
Cfr13I GGNCC 4 cut(s) 4, 86, 711, 721
Cfr42I CCGCGG 1 cut(s) 785
Csp6I GTAC 4 cut(s) 301, 307, 576, 838
CviQI GTAC 4 cut(s) 301, 307, 576, 838
DdeI CTNAG 6 cut(s) 44, 93, 240, 368, 399, 828
DpnI GATC 3 cut(s) 98, 439, 939
DpnII GATC 3 cut(s) 96, 437, 937
DrdI GACNNNNNNGTC 1 cut(s) 65
DriI GACNNNNNGTC 1 cut(s) 178
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 2 cut(s) 184, 339
Eam1105I GACNNNNNGTC 1 cut(s) 178
EciI GGCGGA 1 cut(s) 701
Ecl136II GAGCTC 1 cut(s) 877
Eco130I CCWWGG 2 cut(s) 265, 717
Eco24I GRGCYC 1 cut(s) 879
Eco47I GGWCC 4 cut(s) 4, 86, 711, 721
Eco53kI GAGCTC 1 cut(s) 877
Eco72I CACGTG 1 cut(s) 335
Eco88I CYCGRG 2 cut(s) 380, 552
EcoICRI GAGCTC 1 cut(s) 877
EcoO109I RGGNCCY 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 129, 610
EcoT14I CCWWGG 2 cut(s) 265, 717
EcoT38I GRGCYC 1 cut(s) 879
ErhI CCWWGG 2 cut(s) 265, 717
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 5 cut(s) 339, 549, 776, 779, 782
FokI GGATG 1 cut(s) 711
FriOI GRGCYC 1 cut(s) 879
Fsp4HI GCNGC 5 cut(s) 339, 549, 776, 779, 782
GlaI GCGC 1 cut(s) 754
GluI GCNGC 5 cut(s) 339, 549, 776, 779, 782
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 4 cut(s) 186, 341, 848, 860
HapII CCGG 8 cut(s) 73, 183, 209, 591, 622, 704, 750, 861
HhaI GCGC 1 cut(s) 755
Hin6I GCGC 1 cut(s) 753
HinP1I GCGC 1 cut(s) 753
HincII GTYRAC 2 cut(s) 70, 994
HindII GTYRAC 2 cut(s) 70, 994
HinfI GANTC 5 cut(s) 48, 455, 598, 898, 978
HpaII CCGG 8 cut(s) 73, 183, 209, 591, 622, 704, 750, 861
HphI GGTGA 2 cut(s) 119, 907
Hpy166II GTNNAC 5 cut(s) 70, 714, 823, 931, 994
Hpy188I TCNGA 8 cut(s) 37, 96, 197, 402, 437, 454, 643, 741
Hpy188III TCNNGA 4 cut(s) 25, 522, 880, 975
Hpy8I GTNNAC 5 cut(s) 70, 714, 823, 931, 994
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 266, 850
HpyCH4III ACNGT 2 cut(s) 468, 762
HpyCH4IV ACGT 4 cut(s) 179, 334, 578, 734
HpyCH4V TGCA 4 cut(s) 332, 498, 698, 775
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 344, 554, 563, 781
HpyF3I CTNAG 6 cut(s) 44, 93, 240, 368, 399, 828
HpySE526I ACGT 4 cut(s) 179, 334, 578, 734
HspAI GCGC 1 cut(s) 753
KspI CCGCGG 1 cut(s) 785
Kzo9I GATC 3 cut(s) 96, 437, 937
LmnI GCTCC 6 cut(s) 112, 438, 633, 874, 882, 950
Lsp1109I GCAGC 3 cut(s) 535, 765, 787
MaeII ACGT 4 cut(s) 179, 334, 578, 734
MaeIII GTNAC 3 cut(s) 101, 730, 899
MalI GATC 3 cut(s) 98, 439, 939
MboI GATC 3 cut(s) 96, 437, 937
MboII GAAGA 3 cut(s) 25, 654, 865
MflI RGATCY 1 cut(s) 937
MhlI GDGCHC 3 cut(s) 387, 879, 955
MluCI AATT 4 cut(s) 134, 327, 969, 997
MlyI GAGTC 2 cut(s) 57, 907
MmeI TCCRAC 4 cut(s) 621, 627, 631, 744
MseI TTAA 3 cut(s) 141, 915, 1000
MslI CAYNNNNRTG 4 cut(s) 264, 818, 830, 893
MspA1I CMGCKG 2 cut(s) 778, 784
MspI CCGG 8 cut(s) 73, 183, 209, 591, 622, 704, 750, 861
MspR9I CCNGG 3 cut(s) 131, 612, 623
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 2 cut(s) 131, 612
MvnI CGCG 1 cut(s) 784
MwoI GCNNNNNNNGC 5 cut(s) 338, 344, 554, 563, 781
NciI CCSGG 1 cut(s) 623
NcoI CCATGG 1 cut(s) 265
NdeII GATC 3 cut(s) 96, 437, 937
NlaIV GGNNCC 6 cut(s) 114, 434, 712, 748, 788, 939
NmuCI GTSAC 1 cut(s) 899
NspI RCATGY 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 893
PaeR7I CTCGAG 1 cut(s) 552
PciI ACATGT 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 568
PfeI GAWTC 3 cut(s) 455, 598, 978
PflMI CCANNNNNTGG 1 cut(s) 544
PfoI TCCNGGA 1 cut(s) 621
PkrI GCNGC 5 cut(s) 340, 550, 777, 780, 783
PleI GAGTC 2 cut(s) 56, 906
PmaCI CACGTG 1 cut(s) 335
PmlI CACGTG 1 cut(s) 335
PpsI GAGTC 2 cut(s) 56, 906
Ppu21I YACGTR 2 cut(s) 335, 735
PpuMI RGGWCCY 1 cut(s) 86
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 129, 610
PspCI CACGTG 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 129, 610
PspN4I GGNNCC 6 cut(s) 114, 434, 712, 748, 788, 939
PspPI GGNCC 4 cut(s) 4, 86, 711, 721
PspPPI RGGWCCY 1 cut(s) 86
PspXI VCTCGAGB 1 cut(s) 552
PstI CTGCAG 1 cut(s) 777
PsuI RGATCY 1 cut(s) 937
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 4 cut(s) 302, 308, 577, 839
RsaNI GTAC 4 cut(s) 301, 307, 576, 838
RseI CAYNNNNRTG 4 cut(s) 264, 818, 830, 893
SacI GAGCTC 1 cut(s) 879
SacII CCGCGG 1 cut(s) 785
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 3 cut(s) 141, 915, 1000
SatI GCNGC 5 cut(s) 339, 549, 776, 779, 782
Sau3AI GATC 3 cut(s) 96, 437, 937
Sau96I GGNCC 4 cut(s) 4, 86, 711, 721
ScaI AGTACT 1 cut(s) 839
SchI GAGTC 2 cut(s) 57, 907
ScrFI CCNGG 3 cut(s) 131, 612, 623
SduI GDGCHC 3 cut(s) 387, 879, 955
SfcI CTRYAG 1 cut(s) 773
Sfr274I CTCGAG 1 cut(s) 552
Sfr303I CCGCGG 1 cut(s) 785
SgrBI CCGCGG 1 cut(s) 785
SinI GGWCC 4 cut(s) 4, 86, 711, 721
SlaI CTCGAG 1 cut(s) 552
SmiMI CAYNNNNRTG 4 cut(s) 264, 818, 830, 893
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
Sse9I AATT 4 cut(s) 134, 327, 969, 997
SsiI CCGC 8 cut(s) 293, 338, 388, 566, 686, 782, 784, 941
SstI GAGCTC 1 cut(s) 879
StyD4I CCNGG 3 cut(s) 129, 610, 621
StyI CCWWGG 2 cut(s) 265, 717
TaaI ACNGT 2 cut(s) 468, 762
TaiI ACGT 4 cut(s) 182, 337, 581, 737
TaqI TCGA 2 cut(s) 69, 553
TasI AATT 4 cut(s) 134, 327, 969, 997
TatI WGTACW 2 cut(s) 306, 837
TauI GCSGC 2 cut(s) 341, 784
TfiI GAWTC 3 cut(s) 455, 598, 978
Tru1I TTAA 3 cut(s) 141, 915, 1000
Tru9I TTAA 3 cut(s) 141, 915, 1000
TscAI CASTG 3 cut(s) 705, 832, 895
TseFI GTSAC 1 cut(s) 899
TseI GCWGC 3 cut(s) 548, 775, 778
Tsp45I GTSAC 1 cut(s) 899
TspDTI ATGAA 5 cut(s) 127, 248, 477, 555, 866
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 3 cut(s) 705, 832, 895
Van91I CCANNNNNTGG 1 cut(s) 544
VpaK11BI GGWCC 4 cut(s) 4, 86, 711, 721
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 552
XmiI GTMKAC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.