MD02G1276300.v1.1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000918
Physical Location & Seq
Forward (+)
33143924 .. 33144949
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1276300.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGTCAGTGAAAATAAAAGGCTAGTCGAGGAGGTGTCTGGTTGGCTAAGAGTCTTTGACGATGGTTCAGTCGATCGTAGGTGGACTGGACCTCCTGAGGTCCAGTTCATGGTGGAGCCTGTGGCGCCCCATGATGAATTTGTCGACGGAGTTTCCACAAAAGATGTTGTGGCCGGCAAATCATCTGGACTTCGCATCCGACTATATTTACCGAAGCGAAAGCCGGAGGATGACGACACCAAGCTACCAATAATCCTCCATTTCCACGGGGGTGGCTTTTGCATGAGTCAAGCTGATTGGTACATGTACTACCACATATACACTAAACTATCTCGCTCCACGAATGCCATTGTAGCGTCTCTCTATCAGAGGCTCGCACCGGAGCACCGTCTCCCGGCTGCCATTGACGACGGCTATTCCGCTCTCCTTTGGCTCCGGTCATTGGCTCGAGGCGAGCAAAACGAGCCACTCCTCAGCCACGCAGACTTTAACCGAGTGTTCCTTATTGGGGATAGCACCGGAGGGAACATAGTCCACCACTTGGCGGCTCAAGCGGGGACTGCGGACCTGAGTCCCTTAAGAATTGCCGGCGGGATTTTAATCCACCCCGGTTTCGTACGAGCGGAGCGGAGCAAGTCGGAGTTGGAGCAACCTCAGTCACCTTTCCTTACCCTAGATATGGTGGACAAGTTCTTAGATTTAGCCCTTCCAGTGGGGTCCACCAAGAACCATCCCATAACTTGTCCGATGGGTCCCGAGGCCGCACCGTTGGATAATCTGAAGCTTCCGCCGTTCCTTCTCTGCGTTGCTTCCAAGGACTTGATGGTAGACACGGAAATGGAGTACTTTGAGGCCATGAAGAAGGCCGACAAGGATGTGGAGCTTCTAATCAACGAGGGGGTGAGTCATAGCTTTTATCTGAACAAGATTGCCGTCGACACTGACCCGGACACAGCCGCAGAAACTGAGCGTCTGATTGCAGGGATCAAACAGTTTATCAAGAACACTACGATTGAAGGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

342

Amino Acids

37.88

Weight (kDa)

5.32

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 75 - 187 2.7e-09 BD-FAE
Abhydrolase_3 PF07859 85 - 306 9.7e-50 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 2 cut(s) 109, 541
AccBSI CCGCTC 3 cut(s) 422, 623, 628
AccI GTMKAC 3 cut(s) 144, 828, 936
AclWI GGATC 1 cut(s) 992
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 4 cut(s) 302, 308, 618, 845
AfiI CCNNNNNNNGG 8 cut(s) 109, 394, 442, 508, 541, 544, 679, 712
AflII CTTAAG 1 cut(s) 577
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 1 cut(s) 1016
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 5 cut(s) 244, 293, 784, 883, 912
AluI AGCT 5 cut(s) 244, 293, 784, 883, 912
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 2 cut(s) 363, 395
AlwI GGATC 1 cut(s) 992
AlwNI CAGNNNCTG 1 cut(s) 965
Ama87I CYCGRG 2 cut(s) 447, 755
AoxI GGCC 4 cut(s) 171, 759, 852, 864
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 5 cut(s) 89, 100, 565, 717, 752
AsuC2I CCSGG 3 cut(s) 395, 609, 947
AsuHPI GGTGA 2 cut(s) 651, 913
AvaI CYCGRG 2 cut(s) 447, 755
AvaII GGWCC 5 cut(s) 89, 100, 565, 717, 752
AxyI CCTNAGG 1 cut(s) 96
BanI GGYRCC 1 cut(s) 124
Bbv12I GWGCWC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 385
BccI CCATC 4 cut(s) 56, 738, 742, 817
BceAI ACGGC 3 cut(s) 427, 775, 917
BcnI CCSGG 3 cut(s) 395, 609, 947
BcoDI GTCTC 2 cut(s) 363, 395
BfaI CTAG 2 cut(s) 23, 674
BfoI RGCGCY 1 cut(s) 128
BfrI CTTAAG 1 cut(s) 577
BisI GCNGC 4 cut(s) 399, 546, 762, 957
BlsI GCNGC 4 cut(s) 400, 547, 763, 958
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 3 cut(s) 395, 609, 947
Bme18I GGWCC 5 cut(s) 89, 100, 565, 717, 752
BmeT110I CYCGRG 2 cut(s) 447, 755
BmgT120I GGNCC 5 cut(s) 89, 100, 565, 717, 752
BmiI GGNNCC 6 cut(s) 117, 126, 434, 718, 753, 754
BmrFI CCNGG 3 cut(s) 395, 609, 947
BmsI GCATC 1 cut(s) 204
BoxI GACNNNNGTC 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 3 cut(s) 395, 609, 947
BsaBI GATNNNNATC 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 4 cut(s) 265, 607, 756, 813
BsaWI WCCGGW 3 cut(s) 379, 435, 518
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 394, 442, 508, 541, 544, 679, 712
Bse118I RCCGGY 2 cut(s) 173, 587
Bse1I ACTGG 3 cut(s) 91, 103, 710
Bse21I CCTNAGG 1 cut(s) 96
Bse8I GATNNNNATC 1 cut(s) 599
BseDI CCNNGG 4 cut(s) 265, 607, 756, 813
BseGI GGATG 4 cut(s) 195, 235, 730, 880
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 8 cut(s) 109, 394, 442, 508, 541, 544, 679, 712
BseMII CTCAG 5 cut(s) 87, 487, 560, 668, 957
BseNI ACTGG 3 cut(s) 91, 103, 710
BseRI GAGGAG 2 cut(s) 44, 461
BseXI GCAGC 1 cut(s) 385
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 4 cut(s) 173, 761, 854, 866
BshNI GGYRCC 1 cut(s) 124
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 387
BsiHKCI CYCGRG 2 cut(s) 447, 755
BsiSI CCGG 9 cut(s) 174, 224, 380, 395, 436, 519, 588, 609, 947
BsiWI CGTACG 1 cut(s) 616
BslFI GGGAC 3 cut(s) 558, 571, 738
BslI CCNNNNNNNGG 8 cut(s) 109, 394, 442, 508, 541, 544, 679, 712
BsmAI GTCTC 2 cut(s) 363, 395
BsmBI CGTCTC 2 cut(s) 363, 395
BsmFI GGGAC 3 cut(s) 558, 571, 738
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 4 cut(s) 173, 761, 854, 866
BsoBI CYCGRG 2 cut(s) 447, 755
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 73, 984
BspANI GGCC 4 cut(s) 173, 761, 854, 866
BspCNI CTCAG 5 cut(s) 88, 486, 561, 667, 958
BspLI GGNNCC 6 cut(s) 117, 126, 434, 718, 753, 754
BspPI GGATC 1 cut(s) 992
BspT107I GGYRCC 1 cut(s) 124
BspTI CTTAAG 1 cut(s) 577
BsrBI CCGCTC 3 cut(s) 422, 623, 628
BsrFI RCCGGY 2 cut(s) 173, 587
BsrI ACTGG 3 cut(s) 91, 103, 710
BssAI RCCGGY 2 cut(s) 173, 587
BssECI CCNNGG 4 cut(s) 265, 607, 756, 813
BssMI GATC 2 cut(s) 73, 984
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 813
Bst4CI ACNGT 3 cut(s) 389, 768, 993
BstACI GRCGYC 1 cut(s) 125
BstAFI CTTAAG 1 cut(s) 577
BstC8I GCNNGC 4 cut(s) 175, 375, 455, 589
BstDEI CTNAG 7 cut(s) 47, 96, 473, 569, 654, 694, 966
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 4 cut(s) 195, 235, 730, 880
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 76, 987
BstMAI GTCTC 2 cut(s) 363, 395
BstMBI GATC 2 cut(s) 73, 984
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 5 cut(s) 124, 353, 463, 551, 560
BstNSI RCATGY 1 cut(s) 307
BstPAI GACNNNNGTC 1 cut(s) 570
BstSCI CCNGG 3 cut(s) 393, 607, 945
BstV1I GCAGC 1 cut(s) 385
BstXI CCANNNNNNTGG 1 cut(s) 272
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 4 cut(s) 173, 761, 854, 866
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 4 cut(s) 195, 235, 730, 880
BtsIMutI CAGTG 3 cut(s) 13, 717, 939
Cac8I GCNNGC 4 cut(s) 175, 375, 455, 589
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 1 cut(s) 127
Cfr10I RCCGGY 2 cut(s) 173, 587
Cfr13I GGNCC 5 cut(s) 89, 100, 565, 717, 752
CseI GACGC 2 cut(s) 345, 959
Csp6I GTAC 4 cut(s) 301, 307, 617, 844
CviAII CATG 5 cut(s) 109, 131, 283, 304, 856
CviQI GTAC 4 cut(s) 301, 307, 617, 844
DdeI CTNAG 7 cut(s) 47, 96, 473, 569, 654, 694, 966
DinI GGCGCC 1 cut(s) 126
DpnI GATC 2 cut(s) 75, 986
DpnII GATC 2 cut(s) 73, 984
EaeI YGGCCR 1 cut(s) 171
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 813
Eco47I GGWCC 5 cut(s) 89, 100, 565, 717, 752
Eco57I CTGAAG 1 cut(s) 800
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 447, 755
EcoO109I RGGNCCY 1 cut(s) 752
EcoT14I CCWWGG 1 cut(s) 813
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 813
Esp3I CGTCTC 2 cut(s) 363, 395
FaeI CATG 5 cut(s) 112, 134, 286, 307, 859
FaqI GGGAC 3 cut(s) 558, 571, 738
FatI CATG 5 cut(s) 108, 130, 282, 303, 855
FauI CCCGC 2 cut(s) 547, 584
FblI GTMKAC 3 cut(s) 144, 828, 936
Fnu4HI GCNGC 4 cut(s) 399, 546, 762, 957
FokI GGATG 4 cut(s) 182, 242, 717, 887
Fsp4HI GCNGC 4 cut(s) 399, 546, 762, 957
FspBI CTAG 2 cut(s) 23, 674
GlaI GCGC 1 cut(s) 126
GluI GCNGC 4 cut(s) 399, 546, 762, 957
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 4 cut(s) 173, 761, 854, 866
HapII CCGG 9 cut(s) 174, 224, 380, 395, 436, 519, 588, 609, 947
HgaI GACGC 2 cut(s) 345, 959
HhaI GCGC 1 cut(s) 127
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 5 cut(s) 112, 134, 286, 307, 859
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 2 cut(s) 145, 937
HindII GTYRAC 2 cut(s) 145, 937
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 4 cut(s) 51, 286, 571, 904
HpaII CCGG 9 cut(s) 174, 224, 380, 395, 436, 519, 588, 609, 947
HphI GGTGA 2 cut(s) 651, 913
Hpy166II GTNNAC 7 cut(s) 84, 145, 535, 685, 720, 829, 937
Hpy188I TCNGA 7 cut(s) 200, 369, 640, 747, 780, 921, 975
Hpy188III TCNNGA 4 cut(s) 95, 186, 755, 1000
Hpy8I GTNNAC 7 cut(s) 84, 145, 535, 685, 720, 829, 937
Hpy99I CGWCG 3 cut(s) 149, 413, 938
HpyAV CCTTC 4 cut(s) 716, 806, 856, 1010
HpyCH4III ACNGT 3 cut(s) 389, 768, 993
HpyCH4V TGCA 2 cut(s) 282, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 124, 353, 463, 551, 560
HpyF3I CTNAG 7 cut(s) 47, 96, 473, 569, 654, 694, 966
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 5 cut(s) 112, 134, 286, 307, 859
HspAI GCGC 1 cut(s) 125
KasI GGCGCC 1 cut(s) 124
KflI GGGWCCC 1 cut(s) 752
KroI GCCGGC 2 cut(s) 173, 587
KroNI GCCGGC 2 cut(s) 175, 589
Kzo9I GATC 2 cut(s) 73, 984
LmnI GCTCC 8 cut(s) 115, 341, 382, 438, 625, 630, 646, 880
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 204
MaeI CTAG 2 cut(s) 23, 674
MaeIII GTNAC 1 cut(s) 657
MalI GATC 2 cut(s) 75, 986
MbiI CCGCTC 3 cut(s) 422, 623, 628
MboI GATC 2 cut(s) 73, 984
MboII GAAGA 1 cut(s) 871
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 2 cut(s) 137, 582
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 4 cut(s) 60, 295, 580, 913
MmeI TCCRAC 4 cut(s) 223, 618, 624, 750
MroNI GCCGGC 2 cut(s) 173, 587
MseI TTAA 3 cut(s) 489, 578, 599
MslI CAYNNNNRTG 2 cut(s) 270, 836
MspCI CTTAAG 1 cut(s) 577
MspI CCGG 9 cut(s) 174, 224, 380, 395, 436, 519, 588, 609, 947
MspR9I CCNGG 3 cut(s) 395, 609, 947
Mva1269I GAATGC 1 cut(s) 349
MwoI GCNNNNNNNGC 5 cut(s) 124, 353, 463, 551, 560
NaeI GCCGGC 2 cut(s) 175, 589
NarI GGCGCC 1 cut(s) 125
NciI CCSGG 3 cut(s) 395, 609, 947
NdeII GATC 2 cut(s) 73, 984
NgoMIV GCCGGC 2 cut(s) 173, 587
NlaIII CATG 5 cut(s) 112, 134, 286, 307, 859
NlaIV GGNNCC 6 cut(s) 117, 126, 434, 718, 753, 754
NmuCI GTSAC 1 cut(s) 657
NspI RCATGY 1 cut(s) 307
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 447
PciI ACATGT 1 cut(s) 303
PctI GAATGC 1 cut(s) 349
PdiI GCCGGC 2 cut(s) 175, 589
Pfl23II CGTACG 1 cut(s) 616
PflMI CCANNNNNTGG 2 cut(s) 109, 541
PkrI GCNGC 4 cut(s) 400, 547, 763, 958
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 4 cut(s) 59, 294, 579, 912
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 4 cut(s) 59, 294, 579, 912
PpuMI RGGWCCY 1 cut(s) 752
PscI ACATGT 1 cut(s) 303
PshAI GACNNNNGTC 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 752
PspLI CGTACG 1 cut(s) 616
PspN4I GGNNCC 6 cut(s) 117, 126, 434, 718, 753, 754
PspPI GGNCC 5 cut(s) 89, 100, 565, 717, 752
PspPPI RGGWCCY 1 cut(s) 752
PspXI VCTCGAGB 1 cut(s) 447
PstNI CAGNNNCTG 1 cut(s) 965
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 4 cut(s) 302, 308, 618, 845
RsaNI GTAC 4 cut(s) 301, 307, 617, 844
RseI CAYNNNNRTG 2 cut(s) 270, 836
SalI GTCGAC 2 cut(s) 143, 935
SaqAI TTAA 3 cut(s) 489, 578, 599
SatI GCNGC 4 cut(s) 399, 546, 762, 957
Sau3AI GATC 2 cut(s) 73, 984
Sau96I GGNCC 5 cut(s) 89, 100, 565, 717, 752
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 4 cut(s) 60, 295, 580, 913
ScrFI CCNGG 3 cut(s) 395, 609, 947
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 1 cut(s) 204
SfoI GGCGCC 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 447
SinI GGWCC 5 cut(s) 89, 100, 565, 717, 752
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 270, 836
SmlI CTYRAG 3 cut(s) 447, 549, 577
SmoI CTYRAG 3 cut(s) 447, 549, 577
Sse9I AATT 2 cut(s) 137, 582
SspDI GGCGCC 1 cut(s) 124
SspMI CTAG 2 cut(s) 23, 674
StyD4I CCNGG 3 cut(s) 393, 607, 945
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 3 cut(s) 389, 768, 993
TaqI TCGA 5 cut(s) 27, 72, 144, 448, 936
TasI AATT 2 cut(s) 137, 582
TatI WGTACW 2 cut(s) 306, 843
TauI GCSGC 3 cut(s) 548, 764, 959
Tru1I TTAA 3 cut(s) 489, 578, 599
Tru9I TTAA 3 cut(s) 489, 578, 599
TscAI CASTG 3 cut(s) 13, 717, 946
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 3 cut(s) 97, 150, 872
TspGWI ACGGA 2 cut(s) 162, 848
TspRI CASTG 3 cut(s) 13, 717, 946
Van91I CCANNNNNTGG 2 cut(s) 109, 541
Vha464I CTTAAG 1 cut(s) 577
VpaK11BI GGWCC 5 cut(s) 89, 100, 565, 717, 752
XapI RAATTY 1 cut(s) 137
XceI RCATGY 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 820
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 3 cut(s) 144, 828, 936
XspI CTAG 2 cut(s) 23, 674
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.