RLG00000029966

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000918
Physical Location & Seq
Reverse (-)
52341401 .. 52342405
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029966

Sequence Viewer

Length: 1005 bp
ATGGTCCAAGAAAAGAAAGTGGTCGACGAGGTTTCCGGTTGGCTTAGGCTCTTTGACGACGGATCAGTCGACCGGACATGGACGGGACCCCCTGACGTCAAGTTCATGACGGAACCAGTCCCACCGCACGTTGAGTTCATCGACGGAGTTGCGACAAAAGACATGGTCATCGATGAAACGTCCGGCCTTCGAGTTCGAATTTACTTACCTGAGAGAAAGCCGGAGGATTGTGAGAGTCTGCCCATAATCCTCCACTTCCATGGAGGCGGCTTTTGCATTAGCCAAGCAGATTGGTACATGTACTACCACATGTACACAAACCTCGCACGTACAGCCAACGCCATATGCGTCTCCGTCTATCTACGCCTCGCACCGGAGCACCGTCTCCCGGCTCCGATTGACGATGGCTTCTCTGCTCTCCTCTGGCTCCGATCCCTGGCTCGAGGTGATTCATATGAACCATGGTTCAACGACCATGCAGACTTTAACCGTGTTTACCTCCTGGGGGATAGCACAGGAGGAAACATAGTCCATGAAGTGGCTGCAAGAGCAGGGAAGCTGGATTTGAGTCCGGCGAAATTATCCGGCGGGATTCTTATCCACCCTGGTTTTGTCCGGTCATCGAGGAGCAGGTCAGAGCTGGAACAGCCGCAGTCACCTATGCTTACTCTAGACATGGTGGACAAGTTCCTGAGCTTTGCACTGCCGGTGGGGTCCACCAAGGACCATCCGGTTACGTGTCCGATGGGTTCCGGCGCACCACCGTTGGAAAGTCTGCAGCTGCCGCGGTTCCTCGTGTGTGTGGCAGAGAGGGACATCATGGTGGACACTGAGATGGAATACTATGAGGCCATGAAGAAGGCTGGTAAGGATATGGAGCTCCTGATTAGCACTGGGGTGAGTCACAGTTTTTATCTTAACAAGCTTGCTGTGGACATGGATCCGCAAACAGGAGCACAAACTGCAATCCTAATTTCAAGAATCAAAGAGTTCGTCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

335

Amino Acids

37.31

Weight (kDa)

5.22

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 67 - 178 1.7e-07 BD-FAE
Abhydrolase_3 PF07859 83 - 305 9.5e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 621
AccB7I CCANNNNNTGG 1 cut(s) 538
AccI GTMKAC 2 cut(s) 24, 69
AccII CGCG 1 cut(s) 787
AciI CCGC 7 cut(s) 125, 267, 588, 650, 785, 787, 944
AclWI GGATC 4 cut(s) 70, 426, 935, 948
AcsI RAATTY 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 4 cut(s) 296, 302, 314, 331
AfiI CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
AflIII ACRYGT 3 cut(s) 297, 309, 737
AgsI TTSAA 2 cut(s) 469, 978
AjnI CCWGG 3 cut(s) 435, 501, 604
AleI CACNNNNGTG 1 cut(s) 896
AluBI AGCT 6 cut(s) 559, 640, 696, 781, 880, 925
AluI AGCT 6 cut(s) 559, 640, 696, 781, 880, 925
Alw21I GWGCWC 3 cut(s) 381, 882, 958
Alw26I GTCTC 2 cut(s) 355, 389
AlwI GGATC 4 cut(s) 70, 426, 935, 948
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 2 cut(s) 184, 849
ApeKI GCWGC 3 cut(s) 542, 778, 781
ApoI RAATTY 1 cut(s) 198
ArsI GACNNNNNNTTYG 1 cut(s) 978
AspLEI GCGC 1 cut(s) 758
AspS9I GGNCC 4 cut(s) 4, 86, 714, 724
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 3 cut(s) 458, 648, 910
AsuII TTCGAA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 4 cut(s) 4, 86, 714, 724
BamHI GGATCC 1 cut(s) 940
BanII GRGCYC 1 cut(s) 882
BauI CACGAG 1 cut(s) 794
Bbv12I GWGCWC 3 cut(s) 381, 882, 958
BbvI GCAGC 3 cut(s) 529, 768, 790
BccI CCATC 4 cut(s) 398, 735, 739, 829
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 3 cut(s) 437, 503, 606
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 2 cut(s) 355, 389
BfaI CTAG 1 cut(s) 671
BfmI CTRYAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 621
BisI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
BlsI GCNGC 6 cut(s) 269, 544, 651, 780, 783, 786
Bme1390I CCNGG 4 cut(s) 389, 437, 503, 606
Bme18I GGWCC 4 cut(s) 4, 86, 714, 724
BmeT110I CYCGRG 1 cut(s) 441
BmgT120I GGNCC 4 cut(s) 4, 86, 714, 724
BmiI GGNNCC 9 cut(s) 87, 88, 114, 393, 428, 715, 751, 791, 942
BmrFI CCNGG 4 cut(s) 389, 437, 503, 606
BmrI ACTGGG 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 903
Bpu10I CCTNAGC 2 cut(s) 44, 692
Bpu14I TTCGAA 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 171
BsaAI YACGTR 2 cut(s) 329, 738
BsaBI GATNNNNATC 1 cut(s) 596
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 7 cut(s) 259, 435, 461, 502, 604, 720, 785
BsaWI WCCGGW 5 cut(s) 35, 72, 373, 615, 730
Bsc4I CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 116, 898
Bse8I GATNNNNATC 1 cut(s) 596
BseBI CCWGG 3 cut(s) 437, 503, 606
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 7 cut(s) 259, 435, 461, 502, 604, 720, 785
BseGI GGATG 1 cut(s) 727
BseJI GATNNNNATC 1 cut(s) 596
BseLI CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
BseMII CTCAG 3 cut(s) 201, 683, 822
BseNI ACTGG 2 cut(s) 116, 898
BseRI GAGGAG 2 cut(s) 410, 640
BseXI GCAGC 3 cut(s) 529, 768, 790
Bsh1236I CGCG 1 cut(s) 787
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 2 cut(s) 186, 851
BshVI ATCGAT 1 cut(s) 171
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 3 cut(s) 381, 882, 958
BsiHKCI CYCGRG 1 cut(s) 441
BslFI GGGAC 3 cut(s) 99, 104, 827
BslI CCNNNNNNNGG 6 cut(s) 373, 388, 436, 505, 538, 950
BsmAI GTCTC 2 cut(s) 355, 389
BsmBI CGTCTC 2 cut(s) 355, 389
BsmFI GGGAC 3 cut(s) 99, 104, 827
BsnI GGCC 2 cut(s) 186, 851
BsoBI CYCGRG 1 cut(s) 441
Bsp119I TTCGAA 1 cut(s) 196
Bsp1286I GDGCHC 3 cut(s) 381, 882, 958
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 3 cut(s) 62, 431, 940
Bsp19I CCATGG 2 cut(s) 259, 461
BspACI CCGC 7 cut(s) 125, 267, 588, 650, 785, 787, 944
BspANI GGCC 2 cut(s) 186, 851
BspCNI CTCAG 3 cut(s) 202, 684, 823
BspDI ATCGAT 1 cut(s) 171
BspFNI CGCG 1 cut(s) 787
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 9 cut(s) 87, 88, 114, 393, 428, 715, 751, 791, 942
BspMAI CTGCAG 1 cut(s) 780
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 4 cut(s) 70, 426, 935, 948
BspT104I TTCGAA 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 2 cut(s) 116, 898
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 7 cut(s) 259, 435, 461, 502, 604, 720, 785
BssMI GATC 3 cut(s) 62, 431, 940
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 794
BssT1I CCWWGG 3 cut(s) 259, 461, 720
Bst2BI CACGAG 1 cut(s) 794
Bst2UI CCWGG 3 cut(s) 437, 503, 606
Bst4CI ACNGT 4 cut(s) 383, 491, 765, 908
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 962
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 2 cut(s) 329, 738
BstBI TTCGAA 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 927
BstDEI CTNAG 4 cut(s) 44, 210, 692, 831
BstDSI CCRYGG 3 cut(s) 259, 461, 785
BstF5I GGATG 1 cut(s) 727
BstFNI CGCG 1 cut(s) 787
BstHHI GCGC 1 cut(s) 758
BstKTI GATC 3 cut(s) 65, 434, 943
BstMAI GTCTC 2 cut(s) 355, 389
BstMBI GATC 3 cut(s) 62, 431, 940
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 6 cut(s) 273, 332, 548, 646, 784, 962
BstNI CCWGG 3 cut(s) 437, 503, 606
BstNSI RCATGY 2 cut(s) 301, 313
BstSCI CCNGG 4 cut(s) 387, 435, 501, 604
BstSFI CTRYAG 1 cut(s) 776
BstUI CGCG 1 cut(s) 787
BstV1I GCAGC 3 cut(s) 529, 768, 790
BstX2I RGATCY 1 cut(s) 940
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 940
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 2 cut(s) 186, 851
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 3 cut(s) 259, 461, 785
BtsCI GGATG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 701
BtsIMutI CAGTG 3 cut(s) 701, 828, 891
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 1 cut(s) 927
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 758
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 4 cut(s) 4, 86, 714, 724
Cfr42I CCGCGG 1 cut(s) 788
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 1 cut(s) 337
Csp6I GTAC 4 cut(s) 295, 301, 313, 330
CviQI GTAC 4 cut(s) 295, 301, 313, 330
DdeI CTNAG 4 cut(s) 44, 210, 692, 831
DpnI GATC 3 cut(s) 64, 433, 942
DpnII GATC 3 cut(s) 62, 431, 940
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 3 cut(s) 259, 461, 720
Eco24I GRGCYC 1 cut(s) 882
Eco47I GGWCC 4 cut(s) 4, 86, 714, 724
Eco53kI GAGCTC 1 cut(s) 880
Eco88I CYCGRG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 3 cut(s) 435, 501, 604
EcoT14I CCWWGG 3 cut(s) 259, 461, 720
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 3 cut(s) 259, 461, 720
Esp3I CGTCTC 2 cut(s) 355, 389
FaqI GGGAC 3 cut(s) 99, 104, 827
FauI CCCGC 1 cut(s) 581
FauNDI CATATG 2 cut(s) 344, 454
FblI GTMKAC 2 cut(s) 24, 69
Fnu4HI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
FokI GGATG 1 cut(s) 714
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
FspBI CTAG 1 cut(s) 671
GlaI GCGC 1 cut(s) 757
GluI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
HaeIII GGCC 2 cut(s) 186, 851
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 758
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 1 cut(s) 756
HinP1I GCGC 1 cut(s) 756
HincII GTYRAC 3 cut(s) 25, 70, 997
HindII GTYRAC 3 cut(s) 25, 70, 997
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 6 cut(s) 235, 449, 568, 592, 901, 981
HphI GGTGA 3 cut(s) 458, 648, 910
Hpy166II GTNNAC 9 cut(s) 25, 70, 315, 496, 682, 717, 826, 934, 997
Hpy188I TCNGA 4 cut(s) 396, 431, 637, 744
Hpy188III TCNNGA 5 cut(s) 106, 671, 691, 883, 978
Hpy8I GTNNAC 9 cut(s) 25, 70, 315, 496, 682, 717, 826, 934, 997
Hpy99I CGWCG 3 cut(s) 29, 62, 146
HpyAV CCTTC 2 cut(s) 197, 853
HpyCH4III ACNGT 4 cut(s) 383, 491, 765, 908
HpyCH4IV ACGT 5 cut(s) 96, 129, 179, 328, 737
HpyCH4V TGCA 6 cut(s) 276, 479, 545, 701, 778, 965
HpyF10VI GCNNNNNNNGC 6 cut(s) 273, 332, 548, 646, 784, 962
HpyF3I CTNAG 4 cut(s) 44, 210, 692, 831
HpySE526I ACGT 5 cut(s) 96, 129, 179, 328, 737
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 1 cut(s) 756
KflI GGGWCCC 1 cut(s) 86
KspI CCGCGG 1 cut(s) 788
Kzo9I GATC 3 cut(s) 62, 431, 940
LmnI GCTCC 7 cut(s) 376, 397, 432, 627, 877, 885, 953
Lsp1109I GCAGC 3 cut(s) 529, 768, 790
MaeI CTAG 1 cut(s) 671
MaeII ACGT 5 cut(s) 96, 129, 179, 328, 737
MaeIII GTNAC 3 cut(s) 654, 733, 902
MalI GATC 3 cut(s) 64, 433, 942
MboI GATC 3 cut(s) 62, 431, 940
MboII GAAGA 1 cut(s) 868
MflI RGATCY 1 cut(s) 940
MhlI GDGCHC 3 cut(s) 381, 882, 958
MluCI AATT 4 cut(s) 198, 578, 972, 1000
MlyI GAGTC 3 cut(s) 244, 577, 910
MmeI TCCRAC 1 cut(s) 747
MseI TTAA 3 cut(s) 486, 918, 1003
MslI CAYNNNNRTG 4 cut(s) 258, 821, 833, 896
MspA1I CMGCKG 2 cut(s) 781, 787
MspR9I CCNGG 4 cut(s) 389, 437, 503, 606
MvaI CCWGG 3 cut(s) 437, 503, 606
MvnI CGCG 1 cut(s) 787
MwoI GCNNNNNNNGC 6 cut(s) 273, 332, 548, 646, 784, 962
NciI CCSGG 1 cut(s) 389
NcoI CCATGG 2 cut(s) 259, 461
NdeI CATATG 2 cut(s) 344, 454
NdeII GATC 3 cut(s) 62, 431, 940
NlaIV GGNNCC 9 cut(s) 87, 88, 114, 393, 428, 715, 751, 791, 942
NmuCI GTSAC 2 cut(s) 654, 902
NspI RCATGY 2 cut(s) 301, 313
NspV TTCGAA 1 cut(s) 196
OliI CACNNNNGTG 1 cut(s) 896
PaeR7I CTCGAG 1 cut(s) 441
PagI TCATGA 1 cut(s) 105
PciI ACATGT 2 cut(s) 297, 309
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 345
PfeI GAWTC 3 cut(s) 449, 592, 981
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 1 cut(s) 538
PkrI GCNGC 6 cut(s) 269, 544, 651, 780, 783, 786
PleI GAGTC 3 cut(s) 243, 576, 909
PpsI GAGTC 3 cut(s) 243, 576, 909
Ppu21I YACGTR 2 cut(s) 329, 738
PpuMI RGGWCCY 1 cut(s) 86
PscI ACATGT 2 cut(s) 297, 309
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 3 cut(s) 435, 501, 604
PspGI CCWGG 3 cut(s) 435, 501, 604
PspN4I GGNNCC 9 cut(s) 87, 88, 114, 393, 428, 715, 751, 791, 942
PspPI GGNCC 4 cut(s) 4, 86, 714, 724
PspPPI RGGWCCY 1 cut(s) 86
PspXI VCTCGAGB 1 cut(s) 441
PstI CTGCAG 1 cut(s) 780
PsuI RGATCY 1 cut(s) 940
PsyI GACNNNGTC 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 781
RsaI GTAC 4 cut(s) 296, 302, 314, 331
RsaNI GTAC 4 cut(s) 295, 301, 313, 330
RseI CAYNNNNRTG 4 cut(s) 258, 821, 833, 896
SacI GAGCTC 1 cut(s) 882
SacII CCGCGG 1 cut(s) 788
SalI GTCGAC 2 cut(s) 23, 68
SaqAI TTAA 3 cut(s) 486, 918, 1003
SatI GCNGC 6 cut(s) 268, 543, 650, 779, 782, 785
Sau3AI GATC 3 cut(s) 62, 431, 940
Sau96I GGNCC 4 cut(s) 4, 86, 714, 724
SchI GAGTC 3 cut(s) 244, 577, 910
ScrFI CCNGG 4 cut(s) 389, 437, 503, 606
SduI GDGCHC 3 cut(s) 381, 882, 958
SfcI CTRYAG 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 441
Sfr303I CCGCGG 1 cut(s) 788
SfuI TTCGAA 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 788
SinI GGWCC 4 cut(s) 4, 86, 714, 724
SlaI CTCGAG 1 cut(s) 441
SmiMI CAYNNNNRTG 4 cut(s) 258, 821, 833, 896
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 4 cut(s) 198, 578, 972, 1000
SsiI CCGC 7 cut(s) 125, 267, 588, 650, 785, 787, 944
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 4 cut(s) 387, 435, 501, 604
StyI CCWWGG 3 cut(s) 259, 461, 720
TaaI ACNGT 4 cut(s) 383, 491, 765, 908
TaiI ACGT 5 cut(s) 99, 132, 182, 331, 740
TaqI TCGA 8 cut(s) 24, 69, 141, 171, 190, 196, 442, 623
TasI AATT 4 cut(s) 198, 578, 972, 1000
TatI WGTACW 2 cut(s) 300, 312
TauI GCSGC 3 cut(s) 270, 652, 787
TfiI GAWTC 3 cut(s) 449, 592, 981
Tru1I TTAA 3 cut(s) 486, 918, 1003
Tru9I TTAA 3 cut(s) 486, 918, 1003
TscAI CASTG 3 cut(s) 708, 835, 898
TseFI GTSAC 2 cut(s) 654, 902
TseI GCWGC 3 cut(s) 542, 778, 781
Tsp45I GTSAC 2 cut(s) 654, 902
TspDTI ATGAA 7 cut(s) 94, 127, 189, 441, 471, 549, 869
TspGWI ACGGA 4 cut(s) 75, 125, 159, 343
TspRI CASTG 3 cut(s) 708, 835, 898
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 538
VpaK11BI GGWCC 4 cut(s) 4, 86, 714, 724
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 2 cut(s) 301, 313
XhoI CTCGAG 1 cut(s) 441
XmiI GTMKAC 2 cut(s) 24, 69
XspI CTAG 1 cut(s) 671
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.