pycom02g23580

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000918
Physical Location & Seq
Forward (+)
21676684 .. 21677931
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23580.1

Sequence Viewer

Length: 1065 bp
ATGTATTTTGAATACTTTATTTTTTCATCTTCAGTTTGCTATATTACCATCACCATGGTCAGCGAAAAAAAAAAGCCAGTCGAGGAGGTGTCTGGTTGGCTAAGAGTCTTTGACGATGGCTCAGTCGACCGGAGGTGGACTGGACCTCCTGAGGTTCAGTTCATGGTGAAGCCTGTGGCAGCCCACGATGAATTTGTCGACGGAGTTTCCACAAAAGATGTTATTGCCGACAAATCATCCGGCCTTCGCGTCCGAATATATTTACCGGAACAAAAGCCGGAGAATAATGACACCAAGCTGCCAATAATCCTCCATTTCCACGGAGGTGGCTTTTGCATAAGTCAAGCTGATTGGTACATGTACTACCACATATACACTAAACTATCTCGCTCCACGAATGCCATTGTAATCTCTGTCTATCAGAGGCTCGCACCGGAGCACCGTCTCCCGGCTGCAATCGAGGACGGCTATTCCGCTCTCCTTTGGCTCCGGTCATTGGCTCGAGGTGAGCAAAACGAGCCACACCTCAGCCATGCAGACTATAGCCGTGTGTTTCTTATTGGAGATAGCTCCGGAGGGAACATAGTCCACCACGTGGCGGCTCAAGCGGGGACTGCGGATTTGAGTCCCTTAAAACTTGCCGGCGGGATTCCAATCCACCCCGGTTTTGTTCGAGCGGAGCGGAGTAAGTCGCAATTGGAGCAGCCTCAGTCAACTTTCCTTACCCTAGATATGGTGGACAAGTTTTTAGGGTTAGCCCTTCCAGTGGGGTCCACCAAGGACCATCCCATAACTTGTCCGATAGGTCCCGAGGCCCAACCGTTAGGTAATCTGAAGCTTCCGCCGTTCCTTCTCTGCGTTGCTTCCAAGGACTTGCTGATAGACACGGAAATTGAGTACTTTGAGGCCATGAAGAAGGCTAACAAGGATGTGGAGCTTCTAATCAACGAGGGGGTGAGTCATAGCTTTTATCTGAACAAGATTGCCGTCGACATGGACCCGGAAACAGCTGCAGAAACTGAGCGTCTGATTGCAGGGATCAAACAGTTTATCAAGAACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

355

Amino Acids

39.49

Weight (kDa)

5.81

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 92 - 203 2e-10 BD-FAE
Abhydrolase_3 PF07859 103 - 324 7.3e-50 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 595
AccBSI CCGCTC 3 cut(s) 476, 677, 682
AccI GTMKAC 3 cut(s) 126, 198, 990
AccII CGCG 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 572
AciI CCGC 8 cut(s) 474, 599, 608, 617, 645, 677, 682, 842
AclWI GGATC 1 cut(s) 1046
AcsI RAATTY 1 cut(s) 191
AcuI CTGAAG 2 cut(s) 15, 854
AcvI CACGTG 1 cut(s) 595
AdeI CACNNNGTG 1 cut(s) 595
AfaI GTAC 3 cut(s) 356, 362, 899
AfiI CCNNNNNNNGG 6 cut(s) 448, 496, 595, 598, 733, 766
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 1 cut(s) 11
AleI CACNNNNGTG 1 cut(s) 324
AluBI AGCT 7 cut(s) 298, 347, 570, 838, 937, 966, 1010
AluI AGCT 7 cut(s) 298, 347, 570, 838, 937, 966, 1010
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 1 cut(s) 1046
AlwNI CAGNNNCTG 1 cut(s) 1019
Ama87I CYCGRG 2 cut(s) 501, 809
Aor13HI TCCGGA 1 cut(s) 572
AoxI GGCC 3 cut(s) 241, 813, 906
ApeKI GCWGC 5 cut(s) 179, 298, 452, 703, 1010
ApoI RAATTY 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 604, 636
AspS9I GGNCC 6 cut(s) 143, 771, 781, 806, 814, 997
AsuC2I CCSGG 3 cut(s) 449, 663, 1001
AsuHPI GGTGA 4 cut(s) 43, 178, 518, 967
AvaI CYCGRG 2 cut(s) 501, 809
AvaII GGWCC 5 cut(s) 143, 771, 781, 806, 997
AxyI CCTNAGG 1 cut(s) 150
BbrPI CACGTG 1 cut(s) 595
Bbv12I GWGCWC 1 cut(s) 441
BbvCI CCTCAGC 1 cut(s) 527
BbvI GCAGC 5 cut(s) 191, 285, 439, 715, 997
BccI CCATC 3 cut(s) 56, 110, 792
BceAI ACGGC 4 cut(s) 481, 531, 829, 971
BcnI CCSGG 3 cut(s) 449, 663, 1001
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 728
BfmI CTRYAG 2 cut(s) 541, 1011
BisI GCNGC 6 cut(s) 180, 299, 453, 600, 704, 1011
BlsI GCNGC 6 cut(s) 181, 300, 454, 601, 705, 1012
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 3 cut(s) 449, 663, 1001
Bme18I GGWCC 5 cut(s) 143, 771, 781, 806, 997
BmeT110I CYCGRG 2 cut(s) 501, 809
BmgT120I GGNCC 6 cut(s) 143, 771, 781, 806, 814, 997
BmiI GGNNCC 4 cut(s) 488, 772, 808, 999
BmrFI CCNGG 3 cut(s) 449, 663, 1001
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 588
BpuMI CCSGG 3 cut(s) 449, 663, 1001
BsaAI YACGTR 1 cut(s) 595
BsaBI GATNNNNATC 1 cut(s) 653
BsaJI CCNNGG 6 cut(s) 54, 319, 661, 777, 810, 867
BsaWI WCCGGW 5 cut(s) 129, 265, 433, 489, 572
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 6 cut(s) 448, 496, 595, 598, 733, 766
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 3 cut(s) 77, 145, 764
Bse21I CCTNAGG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 653
BseAI TCCGGA 1 cut(s) 572
BseDI CCNNGG 6 cut(s) 54, 319, 661, 777, 810, 867
BseGI GGATG 3 cut(s) 236, 784, 934
BseJI GATNNNNATC 1 cut(s) 653
BseLI CCNNNNNNNGG 6 cut(s) 448, 496, 595, 598, 733, 766
BseMII CTCAG 5 cut(s) 135, 141, 541, 722, 1011
BseNI ACTGG 3 cut(s) 77, 145, 764
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 5 cut(s) 191, 285, 439, 715, 997
Bsh1236I CGCG 1 cut(s) 249
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 3 cut(s) 243, 815, 908
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 2 cut(s) 501, 809
BslFI GGGAC 3 cut(s) 612, 625, 792
BslI CCNNNNNNNGG 6 cut(s) 448, 496, 595, 598, 733, 766
BsmAI GTCTC 1 cut(s) 449
BsmBI CGTCTC 1 cut(s) 449
BsmFI GGGAC 3 cut(s) 612, 625, 792
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 3 cut(s) 243, 815, 908
BsoBI CYCGRG 2 cut(s) 501, 809
Bsp1286I GDGCHC 1 cut(s) 441
Bsp13I TCCGGA 1 cut(s) 572
Bsp143I GATC 1 cut(s) 1038
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 8 cut(s) 474, 599, 608, 617, 645, 677, 682, 842
BspANI GGCC 3 cut(s) 243, 815, 908
BspCNI CTCAG 5 cut(s) 134, 142, 540, 721, 1012
BspEI TCCGGA 1 cut(s) 572
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 488, 772, 808, 999
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 1 cut(s) 1046
BsrBI CCGCTC 3 cut(s) 476, 677, 682
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 3 cut(s) 77, 145, 764
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 6 cut(s) 54, 319, 661, 777, 810, 867
BssMI GATC 1 cut(s) 1038
BssT1I CCWWGG 3 cut(s) 54, 777, 867
Bst4CI ACNGT 3 cut(s) 443, 822, 1047
BstBAI YACGTR 1 cut(s) 595
BstC8I GCNNGC 2 cut(s) 429, 643
BstDEI CTNAG 6 cut(s) 101, 121, 150, 527, 708, 1020
BstDSI CCRYGG 2 cut(s) 54, 319
BstF5I GGATG 3 cut(s) 236, 784, 934
BstFNI CGCG 1 cut(s) 249
BstKTI GATC 1 cut(s) 1041
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 1038
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 4 cut(s) 517, 605, 614, 700
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 447, 661, 999
BstSFI CTRYAG 2 cut(s) 541, 1011
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 5 cut(s) 191, 285, 439, 715, 997
BstXI CCANNNNNNTGG 2 cut(s) 55, 326
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 3 cut(s) 243, 815, 908
BtgI CCRYGG 2 cut(s) 54, 319
BtsCI GGATG 3 cut(s) 236, 784, 934
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 2 cut(s) 429, 643
CaiI CAGNNNCTG 1 cut(s) 1019
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 6 cut(s) 143, 771, 781, 806, 814, 997
CseI GACGC 2 cut(s) 238, 1013
Csp6I GTAC 3 cut(s) 355, 361, 898
CviAII CATG 6 cut(s) 55, 163, 358, 533, 910, 994
CviQI GTAC 3 cut(s) 355, 361, 898
DdeI CTNAG 6 cut(s) 101, 121, 150, 527, 708, 1020
DpnI GATC 1 cut(s) 1040
DpnII GATC 1 cut(s) 1038
DraIII CACNNNGTG 1 cut(s) 595
EciI GGCGGA 1 cut(s) 831
Eco130I CCWWGG 3 cut(s) 54, 777, 867
Eco47I GGWCC 5 cut(s) 143, 771, 781, 806, 997
Eco57I CTGAAG 2 cut(s) 15, 854
Eco72I CACGTG 1 cut(s) 595
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 2 cut(s) 501, 809
EcoO109I RGGNCCY 1 cut(s) 806
EcoT14I CCWWGG 3 cut(s) 54, 777, 867
ErhI CCWWGG 3 cut(s) 54, 777, 867
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 6 cut(s) 58, 166, 361, 536, 913, 997
FaqI GGGAC 3 cut(s) 612, 625, 792
FatI CATG 6 cut(s) 54, 162, 357, 532, 909, 993
FauI CCCGC 2 cut(s) 601, 638
FblI GTMKAC 3 cut(s) 126, 198, 990
Fnu4HI GCNGC 6 cut(s) 180, 299, 453, 600, 704, 1011
FokI GGATG 3 cut(s) 223, 771, 941
Fsp4HI GCNGC 6 cut(s) 180, 299, 453, 600, 704, 1011
FspBI CTAG 1 cut(s) 728
GluI GCNGC 6 cut(s) 180, 299, 453, 600, 704, 1011
HaeIII GGCC 3 cut(s) 243, 815, 908
HgaI GACGC 2 cut(s) 238, 1013
Hin1II CATG 6 cut(s) 58, 166, 361, 536, 913, 997
HincII GTYRAC 4 cut(s) 127, 199, 714, 991
HindII GTYRAC 4 cut(s) 127, 199, 714, 991
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 4 cut(s) 105, 625, 649, 958
HphI GGTGA 4 cut(s) 43, 178, 518, 967
Hpy166II GTNNAC 8 cut(s) 127, 138, 199, 589, 714, 739, 774, 991
Hpy188I TCNGA 6 cut(s) 254, 423, 801, 834, 975, 1029
Hpy188III TCNNGA 4 cut(s) 149, 573, 809, 1054
Hpy8I GTNNAC 8 cut(s) 127, 138, 199, 589, 714, 739, 774, 991
Hpy99I CGWCG 2 cut(s) 203, 992
HpyAV CCTTC 4 cut(s) 254, 770, 860, 910
HpyCH4III ACNGT 3 cut(s) 443, 822, 1047
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 5 cut(s) 336, 455, 536, 1013, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 517, 605, 614, 700
HpyF3I CTNAG 6 cut(s) 101, 121, 150, 527, 708, 1020
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 6 cut(s) 58, 166, 361, 536, 913, 997
Kpn2I TCCGGA 1 cut(s) 572
KroI GCCGGC 1 cut(s) 641
KroNI GCCGGC 1 cut(s) 643
Kzo9I GATC 1 cut(s) 1038
LmnI GCTCC 7 cut(s) 395, 436, 492, 575, 679, 700, 934
Lsp1109I GCAGC 5 cut(s) 191, 285, 439, 715, 997
MaeI CTAG 1 cut(s) 728
MaeII ACGT 1 cut(s) 594
MalI GATC 1 cut(s) 1040
MbiI CCGCTC 3 cut(s) 476, 677, 682
MboI GATC 1 cut(s) 1038
MboII GAAGA 2 cut(s) 21, 925
MfeI CAATTG 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 3 cut(s) 191, 695, 891
MlyI GAGTC 3 cut(s) 114, 634, 967
MroI TCCGGA 1 cut(s) 572
MroNI GCCGGC 1 cut(s) 641
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 2 cut(s) 53, 324
MspA1I CMGCKG 1 cut(s) 1010
MspR9I CCNGG 3 cut(s) 449, 663, 1001
MunI CAATTG 1 cut(s) 695
Mva1269I GAATGC 1 cut(s) 403
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 4 cut(s) 517, 605, 614, 700
NaeI GCCGGC 1 cut(s) 643
NciI CCSGG 3 cut(s) 449, 663, 1001
NcoI CCATGG 1 cut(s) 54
NdeII GATC 1 cut(s) 1038
NgoMIV GCCGGC 1 cut(s) 641
NlaIII CATG 6 cut(s) 58, 166, 361, 536, 913, 997
NlaIV GGNNCC 4 cut(s) 488, 772, 808, 999
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 324
PaeR7I CTCGAG 1 cut(s) 501
PciI ACATGT 1 cut(s) 357
PctI GAATGC 1 cut(s) 403
PdiI GCCGGC 1 cut(s) 643
PfeI GAWTC 1 cut(s) 649
PflMI CCANNNNNTGG 1 cut(s) 595
PkrI GCNGC 6 cut(s) 181, 300, 454, 601, 705, 1012
PleI GAGTC 3 cut(s) 113, 633, 966
PmaCI CACGTG 1 cut(s) 595
PmlI CACGTG 1 cut(s) 595
PpsI GAGTC 3 cut(s) 113, 633, 966
Ppu21I YACGTR 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 806
PscI ACATGT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 806
PspCI CACGTG 1 cut(s) 595
PspN4I GGNNCC 4 cut(s) 488, 772, 808, 999
PspPI GGNCC 6 cut(s) 143, 771, 781, 806, 814, 997
PspPPI RGGWCCY 1 cut(s) 806
PspXI VCTCGAGB 1 cut(s) 501
PstI CTGCAG 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 1019
PvuII CAGCTG 1 cut(s) 1010
RsaI GTAC 3 cut(s) 356, 362, 899
RsaNI GTAC 3 cut(s) 355, 361, 898
RseI CAYNNNNRTG 2 cut(s) 53, 324
SalI GTCGAC 3 cut(s) 125, 197, 989
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 6 cut(s) 180, 299, 453, 600, 704, 1011
Sau3AI GATC 1 cut(s) 1038
Sau96I GGNCC 6 cut(s) 143, 771, 781, 806, 814, 997
ScaI AGTACT 1 cut(s) 899
SchI GAGTC 3 cut(s) 114, 634, 967
ScrFI CCNGG 3 cut(s) 449, 663, 1001
SduI GDGCHC 1 cut(s) 441
SfcI CTRYAG 2 cut(s) 541, 1011
Sfr274I CTCGAG 1 cut(s) 501
SinI GGWCC 5 cut(s) 143, 771, 781, 806, 997
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 2 cut(s) 53, 324
SmlI CTYRAG 2 cut(s) 501, 603
SmoI CTYRAG 2 cut(s) 501, 603
Sse9I AATT 3 cut(s) 191, 695, 891
SsiI CCGC 8 cut(s) 474, 599, 608, 617, 645, 677, 682, 842
SspMI CTAG 1 cut(s) 728
StyD4I CCNGG 3 cut(s) 447, 661, 999
StyI CCWWGG 3 cut(s) 54, 777, 867
TaaI ACNGT 3 cut(s) 443, 822, 1047
TaiI ACGT 1 cut(s) 597
TaqI TCGA 7 cut(s) 81, 126, 198, 459, 502, 673, 990
TasI AATT 3 cut(s) 191, 695, 891
TatI WGTACW 2 cut(s) 360, 897
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 1 cut(s) 649
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TscAI CASTG 1 cut(s) 771
TseI GCWGC 5 cut(s) 179, 298, 452, 703, 1010
TspDTI ATGAA 4 cut(s) 15, 151, 204, 926
TspGWI ACGGA 3 cut(s) 216, 336, 902
TspRI CASTG 1 cut(s) 771
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 5 cut(s) 143, 771, 781, 806, 997
XapI RAATTY 1 cut(s) 191
XceI RCATGY 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 501
XmiI GTMKAC 3 cut(s) 126, 198, 990
XspI CTAG 1 cut(s) 728
ZrmI AGTACT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.