MD02G1275600.v1.1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000918
Physical Location & Seq
Forward (+)
32999727 .. 33000065
339 bp
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UTR
Exon/CDS
Intron
MD02G1275600.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGTGGACAAGTTGTTAGGGTTAGCCATTCCAGCGGGGTCCACCAAGAACCATCCCATAACTTGTCCGATGGGTCCCAAGGCCCCACCGTTGGATAATTTGAAGCTTCCGCCGTTCCTTCTCTGCGTTGCTTCCAAGGACTTGATCATAGACACGCAAATGGAGTACTTTGAGGCCATGAAGAAGGCCAACAAGGATGTGGAACTTCTAATCAACGAGGGGGTGACTCATTGCTTTTATCTGAACATAATTGCTGTGGACACGGACCCGGAAACAGCCGCAGAAACTGAGCGTCTGATTGCAGGGATCAAACAGTTTATCAAGAACCACTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

113

Amino Acids

12.4

Weight (kDa)

5.28

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 2 - 80 1.8e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 35, 110, 279
AclWI GGATC 1 cut(s) 314
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 1 cut(s) 103
AluBI AGCT 1 cut(s) 106
AluI AGCT 1 cut(s) 106
AlwI GGATC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 3 cut(s) 81, 174, 186
AspS9I GGNCC 4 cut(s) 39, 74, 82, 265
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 3 cut(s) 39, 74, 265
BccI CCATC 2 cut(s) 60, 64
BceAI ACGGC 1 cut(s) 97
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 1 cut(s) 269
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
BmcAI AGTACT 1 cut(s) 167
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 3 cut(s) 39, 74, 265
BmgT120I GGNCC 4 cut(s) 39, 74, 82, 265
BmiI GGNNCC 5 cut(s) 40, 75, 76, 84, 267
BmrFI CCNGG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 78, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 229
BseDI CCNNGG 2 cut(s) 78, 135
BseGI GGATG 2 cut(s) 52, 202
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 279
BshFI GGCC 3 cut(s) 83, 176, 188
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 91
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 3 cut(s) 83, 176, 188
Bsp143I GATC 2 cut(s) 144, 306
BspACI CCGC 3 cut(s) 35, 110, 279
BspANI GGCC 3 cut(s) 83, 176, 188
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 5 cut(s) 40, 75, 76, 84, 267
BspPI GGATC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 78, 135
BssMI GATC 2 cut(s) 144, 306
BssT1I CCWWGG 2 cut(s) 78, 135
Bst4CI ACNGT 2 cut(s) 90, 315
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 52, 202
BstKTI GATC 2 cut(s) 147, 309
BstMBI GATC 2 cut(s) 144, 306
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 267
BsuRI GGCC 3 cut(s) 83, 176, 188
BtsCI GGATG 2 cut(s) 52, 202
CaiI CAGNNNCTG 1 cut(s) 287
Cfr13I GGNCC 4 cut(s) 39, 74, 82, 265
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 166
CspCI CAANNNNNGTGG 1 cut(s) 317
CviAII CATG 1 cut(s) 178
CviJI RGCY 6 cut(s) 26, 83, 106, 176, 188, 278
CviKI_1 RGCY 6 cut(s) 26, 83, 106, 176, 188, 278
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 146, 308
DpnII GATC 2 cut(s) 144, 306
EciI GGCGGA 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 78, 135
Eco47I GGWCC 3 cut(s) 39, 74, 265
EcoO109I RGGNCCY 2 cut(s) 74, 82
EcoT14I CCWWGG 2 cut(s) 78, 135
ErhI CCWWGG 2 cut(s) 78, 135
FaeI CATG 1 cut(s) 181
FaiI YATR 5 cut(s) 59, 149, 179, 248, 333
FaqI GGGAC 1 cut(s) 60
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 28
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 2 cut(s) 39, 209
Fsp4HI GCNGC 1 cut(s) 279
GluI GCNGC 1 cut(s) 279
HaeIII GGCC 3 cut(s) 83, 176, 188
HapII CCGG 1 cut(s) 269
HgaI GACGC 1 cut(s) 281
Hin1II CATG 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 1 cut(s) 226
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 7, 42, 259
Hpy188I TCNGA 3 cut(s) 69, 243, 297
Hpy188III TCNNGA 1 cut(s) 322
Hpy8I GTNNAC 3 cut(s) 7, 42, 259
HpyAV CCTTC 2 cut(s) 128, 178
HpyCH4III ACNGT 2 cut(s) 90, 315
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 1 cut(s) 181
KflI GGGWCCC 1 cut(s) 74
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 2 cut(s) 144, 306
LpnPI CCDG 3 cut(s) 45, 282, 288
MaeIII GTNAC 1 cut(s) 223
MalI GATC 2 cut(s) 146, 308
MboI GATC 2 cut(s) 144, 306
MboII GAAGA 1 cut(s) 193
MluCI AATT 2 cut(s) 97, 249
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 2 cut(s) 166, 211
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 32
NciI CCSGG 1 cut(s) 269
NdeII GATC 2 cut(s) 144, 306
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 5 cut(s) 40, 75, 76, 84, 267
NmuCI GTSAC 1 cut(s) 223
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
PspN4I GGNNCC 5 cut(s) 40, 75, 76, 84, 267
PspPI GGNCC 4 cut(s) 39, 74, 82, 265
PspPPI RGGWCCY 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
RseI CAYNNNNRTG 1 cut(s) 158
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 2 cut(s) 144, 306
Sau96I GGNCC 4 cut(s) 39, 74, 82, 265
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 269
SetI ASST 1 cut(s) 108
SinI GGWCC 3 cut(s) 39, 74, 265
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 2 cut(s) 97, 249
SsiI CCGC 3 cut(s) 35, 110, 279
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 78, 135
TaaI ACNGT 2 cut(s) 90, 315
TasI AATT 2 cut(s) 97, 249
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 281
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 39, 74, 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.