RchiOBHm_Chr1g0327831

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000918
Physical Location & Seq
Reverse (-)
16772395 .. 16772862
468 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55732

Sequence Viewer

Length: 468 bp
ATGAAGTGGCTGCTCGAGCAGGGAATGCGGATTTGGGTAAGTTCAAGTTATCCGGTGGGATTCTTTATCCCACCTGGCTTTGTCCGGGAAACGAGGAGCAAGTCGGAGTTGGAACAGCCCCAGTCTTCGTTTTTGAGCGTAGATATGGCGGACAAGTTATTCAGCTTGTCACTTCCGGTGGGATCAACCAAGGACCACCCGATTACGTGTCCGATGGGTTCATTGGCCCCGCCTTTGGATGGTCTGAAGCTGCCTCCGATGCTTGTTTTCGTTGGGGAGATCGACGTCTTTAAGGACACTCAGGTGGAATACTACGAGGCCATGAAGAAGGCTAACCAGGATGTGCAGCTTTTGAGTTTCCCCGGAATGGGTCATGCTTTTTATCTTTCGGCGGAAATGAACCCATGCACGTCTGTCCAAACTGCGAGTCTAGTTTCTGGGATCGCAGAGTTTGTCAAGAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

155

Amino Acids

17.18

Weight (kDa)

5.56

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 28 - 128 5.6e-16 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AciI CCGC 4 cut(s) 28, 149, 230, 392
AclWI GGATC 2 cut(s) 190, 449
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 285
AfiI CCNNNNNNNGG 4 cut(s) 235, 239, 367, 368
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 1 cut(s) 45
AjiI CACGTC 1 cut(s) 411
AjnI CCWGG 2 cut(s) 73, 336
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
AleI CACNNNNGTG 1 cut(s) 302
AluBI AGCT 3 cut(s) 165, 250, 349
AluI AGCT 3 cut(s) 165, 250, 349
AlwI GGATC 2 cut(s) 190, 449
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 2 cut(s) 225, 318
ApeKI GCWGC 3 cut(s) 10, 250, 346
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
AspS9I GGNCC 2 cut(s) 193, 226
AsuC2I CCSGG 2 cut(s) 86, 363
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 237, 358
BccI CCATC 2 cut(s) 208, 233
BciT130I CCWGG 2 cut(s) 75, 338
BcnI CCSGG 2 cut(s) 86, 363
BfaI CTAG 1 cut(s) 431
BisI GCNGC 3 cut(s) 11, 251, 347
BlsI GCNGC 3 cut(s) 12, 252, 348
Bme1390I CCNGG 4 cut(s) 75, 86, 338, 363
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 14
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 2 cut(s) 193, 226
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 4 cut(s) 75, 86, 338, 363
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 117
BpuMI CCSGG 2 cut(s) 86, 363
BsaAI YACGTR 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 189, 361
BsaWI WCCGGW 2 cut(s) 52, 175
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 239, 367, 368
Bse1I ACTGG 1 cut(s) 121
BseBI CCWGG 2 cut(s) 75, 338
BseDI CCNNGG 2 cut(s) 189, 361
BseGI GGATG 2 cut(s) 244, 346
BseLI CCNNNNNNNGG 4 cut(s) 235, 239, 367, 368
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 2 cut(s) 237, 358
BsgI GTGCAG 1 cut(s) 365
BshFI GGCC 2 cut(s) 227, 320
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 4 cut(s) 53, 85, 176, 363
BslI CCNNNNNNNGG 4 cut(s) 235, 239, 367, 368
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 2 cut(s) 227, 320
BsoBI CYCGRG 1 cut(s) 14
Bsp143I GATC 3 cut(s) 182, 279, 441
BspACI CCGC 4 cut(s) 28, 149, 230, 392
BspANI GGCC 2 cut(s) 227, 320
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 2 cut(s) 190, 449
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 189, 361
BssMI GATC 3 cut(s) 182, 279, 441
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 2 cut(s) 75, 338
BstACI GRCGYC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 300
BstF5I GGATG 2 cut(s) 244, 346
BstKTI GATC 3 cut(s) 185, 282, 444
BstMBI GATC 3 cut(s) 182, 279, 441
BstMWI GCNNNNNNNGC 3 cut(s) 16, 25, 259
BstNI CCWGG 2 cut(s) 75, 338
BstSCI CCNGG 4 cut(s) 73, 84, 336, 361
BstV1I GCAGC 2 cut(s) 237, 358
BstV2I GAAGAC 1 cut(s) 117
BsuRI GGCC 2 cut(s) 227, 320
BtrI CACGTC 1 cut(s) 411
BtsCI GGATG 2 cut(s) 244, 346
Cfr13I GGNCC 2 cut(s) 193, 226
CviAII CATG 3 cut(s) 322, 374, 405
CviJI RGCY 9 cut(s) 10, 78, 118, 165, 227, 250, 320, 332, 349
CviKI_1 RGCY 9 cut(s) 10, 78, 118, 165, 227, 250, 320, 332, 349
DdeI CTNAG 1 cut(s) 300
DpnI GATC 3 cut(s) 184, 281, 443
DpnII GATC 3 cut(s) 182, 279, 441
EciI GGCGGA 2 cut(s) 164, 407
Eco130I CCWWGG 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 73, 336
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 3 cut(s) 325, 377, 408
FaiI YATR 4 cut(s) 146, 323, 375, 406
FatI CATG 3 cut(s) 321, 373, 404
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 11, 251, 347
FokI GGATG 2 cut(s) 251, 353
Fsp4HI GCNGC 3 cut(s) 11, 251, 347
FspBI CTAG 1 cut(s) 431
GluI GCNGC 3 cut(s) 11, 251, 347
HaeIII GGCC 2 cut(s) 227, 320
HapII CCGG 4 cut(s) 53, 85, 176, 363
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 3 cut(s) 325, 377, 408
HinfI GANTC 2 cut(s) 60, 427
HpaII CCGG 4 cut(s) 53, 85, 176, 363
Hpy188I TCNGA 4 cut(s) 106, 213, 246, 258
Hpy188III TCNNGA 1 cut(s) 457
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 1 cut(s) 322
HpyCH4IV ACGT 3 cut(s) 206, 285, 410
HpyCH4V TGCA 2 cut(s) 346, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 25, 259
HpyF3I CTNAG 1 cut(s) 300
HpySE526I ACGT 3 cut(s) 206, 285, 410
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 3 cut(s) 325, 377, 408
Kzo9I GATC 3 cut(s) 182, 279, 441
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 237, 358
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 431
MaeII ACGT 3 cut(s) 206, 285, 410
MaeIII GTNAC 1 cut(s) 168
MalI GATC 3 cut(s) 184, 281, 443
MboI GATC 3 cut(s) 182, 279, 441
MboII GAAGA 2 cut(s) 117, 337
MlyI GAGTC 1 cut(s) 436
MmeI TCCRAC 2 cut(s) 84, 90
MnlI CCTC 3 cut(s) 87, 264, 310
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 302
MspI CCGG 4 cut(s) 53, 85, 176, 363
MspR9I CCNGG 4 cut(s) 75, 86, 338, 363
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 2 cut(s) 75, 338
MwoI GCNNNNNNNGC 3 cut(s) 16, 25, 259
NciI CCSGG 2 cut(s) 86, 363
NdeII GATC 3 cut(s) 182, 279, 441
NlaIII CATG 3 cut(s) 325, 377, 408
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 168
OliI CACNNNNGTG 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 14
PctI GAATGC 1 cut(s) 30
PfeI GAWTC 1 cut(s) 60
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 3 cut(s) 12, 252, 348
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Ppu21I YACGTR 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 73, 336
PspGI CCWGG 2 cut(s) 73, 336
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 2 cut(s) 193, 226
PspXI VCTCGAGB 1 cut(s) 14
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 3 cut(s) 11, 251, 347
Sau3AI GATC 3 cut(s) 182, 279, 441
Sau96I GGNCC 2 cut(s) 193, 226
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 4 cut(s) 75, 86, 338, 363
SetI ASST 8 cut(s) 76, 167, 209, 252, 288, 306, 351, 413
SfaNI GCATC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
SsiI CCGC 4 cut(s) 28, 149, 230, 392
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 4 cut(s) 73, 84, 336, 361
StyI CCWWGG 1 cut(s) 189
TaiI ACGT 3 cut(s) 209, 288, 413
TaqI TCGA 2 cut(s) 15, 282
TfiI GAWTC 1 cut(s) 60
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 3 cut(s) 10, 250, 346
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 4 cut(s) 17, 210, 338, 413
VpaK11BI GGWCC 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 14
XspI CTAG 1 cut(s) 431
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.