pycom07g03080

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000918
Physical Location & Seq
Reverse (-)
2550125 .. 2551126
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03080.1

Sequence Viewer

Length: 1002 bp
ATGGTCTGCCGGAAGGTTATTGTCGATGAGGTGTCCGGTTGGCTGAGAGTGTTCGCTGATGGCTCAGTTGATCGGACGTGGACTGGACCGCCTCAGGTCCAGTTCATGACTGAGCCAGTTGCACCTCACGATGAATTCATTGATGGAGTTGCCACCCGAGATGTTTACGTTAACGAAAACCTCCGGCTACGAATTTATCTACCGGAGACAAACCCGGAAGATTCTTCGAAGCTCCCCATAATCCTTCACCTCCATGGAGGTGGCTTTTGTATTAGCCAAGCGGATTGGTACATGTACTACCAAATGTACACCAAGCTTGCACGCTCAGCCAAAGCCATTTGCATCTCGGTCTACTTAAGTCTCGCGCCCGAGCACCGTCTCCCCGCTCCAATCATCGACGGCTTCTCTGCGTTGCTGTGGCTTCGTTCCGTTGCACAAGGCGAGTCATACGAGCAATGGCTAGTCAGCCACGCAGACTTCAACCGTGTCTTTCTCATAGGGGACAGCTCGGGTGGAAATCTCGTGCATGAAATTGCCGCTCGAGCAGGGAAGGTGGATTTGAGTCCGTTAAGGCTAGCGGGTGGGATTCCTATCCACCCAGGCTTCGTTCGAGCGGTGAGGAGTAGGTCGGAGTTGGAGCAGCCAGAGTCACCTTTGCTAACCCTAGACATGGTGGACAAGTTTTTGAGCTTGGCACTGCCAGTGGGGTCGACCAAGGACCATCCAATAACTTGTCCGATGGGCCCCGAAGCATCGCCTTTGGATACTCTGAAGCTGCCGCCGTTTCTTCTATGTATAGCTGAGATGGACATGATAATAGACACTGAGATGGAATACTATGACGCTATGAAGAAGGCTAAGAAGGATGTGGAGCTTCTGATTAGTCCAGGGATGAGTCATAGCTTTTATCTTAACAAGATTGCGGTGGATATGGATCCTCAAACGGCTGCGCAAACTGAGGCTCTAATTTCAGGGATCAAAGACTTTGTCAACAAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

334

Amino Acids

37.04

Weight (kDa)

5.29

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 64 - 176 2.3e-08 BD-FAE
Abhydrolase_3 PF07859 81 - 303 2.1e-47 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 951
AccBSI CCGCTC 3 cut(s) 386, 539, 614
AccI GTMKAC 2 cut(s) 351, 710
AccII CGCG 1 cut(s) 365
AciI CCGC 8 cut(s) 89, 281, 384, 537, 578, 614, 779, 923
AclWI GGATC 3 cut(s) 929, 942, 983
AcsI RAATTY 2 cut(s) 134, 192
AcuI CTGAAG 1 cut(s) 791
AfaI GTAC 3 cut(s) 290, 296, 308
AfiI CCNNNNNNNGG 1 cut(s) 670
AflII CTTAAG 1 cut(s) 355
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 1 cut(s) 481
AjiI CACGTC 1 cut(s) 78
AjnI CCWGG 2 cut(s) 598, 886
AluBI AGCT 8 cut(s) 232, 316, 507, 690, 775, 800, 874, 903
AluI AGCT 8 cut(s) 232, 316, 507, 690, 775, 800, 874, 903
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 3 cut(s) 200, 365, 383
AlwI GGATC 3 cut(s) 929, 942, 983
Ama87I CYCGRG 4 cut(s) 156, 368, 508, 540
AoxI GGCC 1 cut(s) 742
ApaI GGGCCC 1 cut(s) 746
ApeKI GCWGC 3 cut(s) 640, 775, 947
ApoI RAATTY 2 cut(s) 134, 192
ArsI GACNNNNNNTTYG 1 cut(s) 972
AspLEI GCGC 2 cut(s) 367, 952
AspS9I GGNCC 5 cut(s) 86, 97, 718, 742, 743
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 239, 628, 642
AsuII TTCGAA 1 cut(s) 227
AsuNHI GCTAGC 1 cut(s) 574
AvaI CYCGRG 4 cut(s) 156, 368, 508, 540
AvaII GGWCC 3 cut(s) 86, 97, 718
AxyI CCTNAGG 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 746
BamHI GGATCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 746
BauI CACGAG 1 cut(s) 521
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 652, 762, 934
BccI CCATC 6 cut(s) 53, 137, 729, 733, 799, 823
BceAI ACGGC 3 cut(s) 415, 766, 960
BciT130I CCWGG 2 cut(s) 600, 888
BciVI GTATCC 1 cut(s) 757
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 3 cut(s) 200, 365, 383
BfaI CTAG 3 cut(s) 461, 575, 665
BfrI CTTAAG 1 cut(s) 355
BfuI GTATCC 1 cut(s) 757
BisI GCNGC 5 cut(s) 537, 641, 776, 779, 948
BlpI GCTNAGC 1 cut(s) 325
BlsI GCNGC 5 cut(s) 538, 642, 777, 780, 949
Bme1390I CCNGG 3 cut(s) 215, 600, 888
Bme18I GGWCC 3 cut(s) 86, 97, 718
BmeT110I CYCGRG 4 cut(s) 156, 368, 508, 540
BmgBI CACGTC 1 cut(s) 78
BmgT120I GGNCC 5 cut(s) 86, 97, 718, 742, 743
BmiI GGNNCC 3 cut(s) 744, 745, 936
BmrFI CCNGG 3 cut(s) 215, 600, 888
BmsI GCATC 2 cut(s) 351, 761
BmtI GCTAGC 1 cut(s) 578
Bpu1102I GCTNAGC 1 cut(s) 325
Bpu14I TTCGAA 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 215
BsaBI GATNNNNATC 2 cut(s) 590, 933
BsaJI CCNNGG 4 cut(s) 253, 598, 714, 887
BsaWI WCCGGW 2 cut(s) 35, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse1I ACTGG 4 cut(s) 88, 100, 116, 701
Bse21I CCTNAGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 461
Bse8I GATNNNNATC 2 cut(s) 590, 933
BseBI CCWGG 2 cut(s) 600, 888
BseDI CCNNGG 4 cut(s) 253, 598, 714, 887
BseGI GGATG 3 cut(s) 721, 871, 897
BseJI GATNNNNATC 2 cut(s) 590, 933
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMI GCAATG 1 cut(s) 461
BseMII CTCAG 8 cut(s) 35, 78, 102, 107, 339, 792, 816, 948
BseNI ACTGG 4 cut(s) 88, 100, 116, 701
BseRI GAGGAG 1 cut(s) 634
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 3 cut(s) 652, 762, 934
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 1 cut(s) 744
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 4 cut(s) 156, 368, 508, 540
BsiSI CCGG 5 cut(s) 10, 36, 184, 203, 215
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 3 cut(s) 200, 365, 383
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 1 cut(s) 744
BsoBI CYCGRG 4 cut(s) 156, 368, 508, 540
Bsp119I TTCGAA 1 cut(s) 227
Bsp120I GGGCCC 1 cut(s) 742
Bsp1286I GDGCHC 2 cut(s) 375, 746
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 3 cut(s) 70, 934, 975
Bsp1720I GCTNAGC 1 cut(s) 325
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 8 cut(s) 89, 281, 384, 537, 578, 614, 779, 923
BspANI GGCC 1 cut(s) 744
BspCNI CTCAG 8 cut(s) 36, 77, 103, 106, 338, 793, 817, 949
BspFNI CGCG 1 cut(s) 365
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 3 cut(s) 744, 745, 936
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 3 cut(s) 929, 942, 983
BspT104I TTCGAA 1 cut(s) 227
BspTI CTTAAG 1 cut(s) 355
BsrBI CCGCTC 3 cut(s) 386, 539, 614
BsrDI GCAATG 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 4 cut(s) 88, 100, 116, 701
BssECI CCNNGG 4 cut(s) 253, 598, 714, 887
BssMI GATC 3 cut(s) 70, 934, 975
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 2 cut(s) 253, 714
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 2 cut(s) 600, 888
Bst4CI ACNGT 2 cut(s) 377, 485
BstAFI CTTAAG 1 cut(s) 355
BstAUI TGTACA 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 3 cut(s) 318, 322, 576
BstDEI CTNAG 9 cut(s) 44, 64, 93, 111, 325, 801, 825, 858, 957
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 3 cut(s) 721, 871, 897
BstFNI CGCG 1 cut(s) 365
BstHHI GCGC 2 cut(s) 367, 952
BstKTI GATC 3 cut(s) 73, 937, 978
BstMAI GTCTC 3 cut(s) 200, 365, 383
BstMBI GATC 3 cut(s) 70, 934, 975
BstMWI GCNNNNNNNGC 2 cut(s) 326, 542
BstNI CCWGG 2 cut(s) 600, 888
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 3 cut(s) 213, 598, 886
BstSLI GKGCMC 1 cut(s) 746
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 652, 762, 934
BstX2I RGATCY 1 cut(s) 934
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 934
Bsu36I CCTNAGG 1 cut(s) 93
BsuI GTATCC 1 cut(s) 757
BsuRI GGCC 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 738
BtrI CACGTC 1 cut(s) 78
BtsCI GGATG 3 cut(s) 721, 871, 897
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 3 cut(s) 695, 708, 822
Cac8I GCNNGC 3 cut(s) 318, 322, 576
CciI TCATGA 1 cut(s) 105
CfoI GCGC 2 cut(s) 367, 952
Cfr13I GGNCC 5 cut(s) 86, 97, 718, 742, 743
CseI GACGC 1 cut(s) 851
Csp6I GTAC 3 cut(s) 289, 295, 307
CviAII CATG 6 cut(s) 106, 254, 292, 527, 670, 811
CviQI GTAC 3 cut(s) 289, 295, 307
DdeI CTNAG 9 cut(s) 44, 64, 93, 111, 325, 801, 825, 858, 957
DpnI GATC 3 cut(s) 72, 936, 977
DpnII GATC 3 cut(s) 70, 934, 975
Eco130I CCWWGG 2 cut(s) 253, 714
Eco24I GRGCYC 1 cut(s) 746
Eco47I GGWCC 3 cut(s) 86, 97, 718
Eco57I CTGAAG 1 cut(s) 791
Eco81I CCTNAGG 1 cut(s) 93
Eco88I CYCGRG 4 cut(s) 156, 368, 508, 540
EcoO109I RGGNCCY 1 cut(s) 743
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 598, 886
EcoT14I CCWWGG 2 cut(s) 253, 714
EcoT38I GRGCYC 1 cut(s) 746
ErhI CCWWGG 2 cut(s) 253, 714
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 6 cut(s) 109, 257, 295, 530, 673, 814
FaqI GGGAC 1 cut(s) 515
FatI CATG 6 cut(s) 105, 253, 291, 526, 669, 810
FauI CCCGC 2 cut(s) 391, 571
FblI GTMKAC 2 cut(s) 351, 710
Fnu4HI GCNGC 5 cut(s) 537, 641, 776, 779, 948
FokI GGATG 3 cut(s) 708, 878, 904
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 5 cut(s) 537, 641, 776, 779, 948
FspBI CTAG 3 cut(s) 461, 575, 665
FspI TGCGCA 1 cut(s) 951
GlaI GCGC 2 cut(s) 366, 951
GluI GCNGC 5 cut(s) 537, 641, 776, 779, 948
HaeIII GGCC 1 cut(s) 744
HapII CCGG 5 cut(s) 10, 36, 184, 203, 215
HgaI GACGC 1 cut(s) 851
HhaI GCGC 2 cut(s) 367, 952
Hin1II CATG 6 cut(s) 109, 257, 295, 530, 673, 814
Hin6I GCGC 2 cut(s) 365, 950
HinP1I GCGC 2 cut(s) 365, 950
HincII GTYRAC 3 cut(s) 172, 711, 991
HindII GTYRAC 3 cut(s) 172, 711, 991
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 6 cut(s) 221, 443, 562, 586, 647, 895
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 5 cut(s) 10, 36, 184, 203, 215
HphI GGTGA 3 cut(s) 239, 628, 642
Hpy166II GTNNAC 8 cut(s) 81, 166, 172, 309, 352, 676, 711, 991
Hpy188I TCNGA 5 cut(s) 75, 631, 738, 771, 879
Hpy188III TCNNGA 2 cut(s) 106, 128
Hpy8I GTNNAC 8 cut(s) 81, 166, 172, 309, 352, 676, 711, 991
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 5 cut(s) 7, 254, 544, 847, 856
HpyCH4III ACNGT 2 cut(s) 377, 485
HpyCH4IV ACGT 2 cut(s) 77, 168
HpyCH4V TGCA 5 cut(s) 122, 320, 342, 434, 526
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 542
HpyF3I CTNAG 9 cut(s) 44, 64, 93, 111, 325, 801, 825, 858, 957
HpySE526I ACGT 2 cut(s) 77, 168
Hsp92II CATG 6 cut(s) 109, 257, 295, 530, 673, 814
HspAI GCGC 2 cut(s) 365, 950
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 3 cut(s) 70, 934, 975
LmnI GCTCC 4 cut(s) 237, 391, 637, 871
Lsp1109I GCAGC 3 cut(s) 652, 762, 934
LweI GCATC 2 cut(s) 351, 761
MaeI CTAG 3 cut(s) 461, 575, 665
MaeII ACGT 2 cut(s) 77, 168
MaeIII GTNAC 1 cut(s) 648
MalI GATC 3 cut(s) 72, 936, 977
MbiI CCGCTC 3 cut(s) 386, 539, 614
MboI GATC 3 cut(s) 70, 934, 975
MboII GAAGA 4 cut(s) 216, 230, 779, 862
MflI RGATCY 1 cut(s) 934
MhlI GDGCHC 2 cut(s) 375, 746
MluCI AATT 4 cut(s) 134, 192, 531, 966
MlyI GAGTC 4 cut(s) 452, 571, 656, 904
MmeI TCCRAC 2 cut(s) 609, 615
MnlI CCTC 9 cut(s) 22, 102, 135, 191, 251, 260, 612, 948, 952
MseI TTAA 4 cut(s) 171, 356, 569, 912
MslI CAYNNNNRTG 3 cut(s) 252, 258, 827
MspCI CTTAAG 1 cut(s) 355
MspI CCGG 5 cut(s) 10, 36, 184, 203, 215
MspR9I CCNGG 3 cut(s) 215, 600, 888
MvaI CCWGG 2 cut(s) 600, 888
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 2 cut(s) 326, 542
NciI CCSGG 1 cut(s) 215
NcoI CCATGG 1 cut(s) 253
NdeII GATC 3 cut(s) 70, 934, 975
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 6 cut(s) 109, 257, 295, 530, 673, 814
NlaIV GGNNCC 3 cut(s) 744, 745, 936
NmuCI GTSAC 1 cut(s) 648
NsbI TGCGCA 1 cut(s) 951
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 227
PaeR7I CTCGAG 1 cut(s) 540
PagI TCATGA 1 cut(s) 105
PciI ACATGT 1 cut(s) 291
PfeI GAWTC 2 cut(s) 221, 586
PflFI GACNNNGTC 1 cut(s) 986
PkrI GCNGC 5 cut(s) 538, 642, 777, 780, 949
PleI GAGTC 4 cut(s) 451, 570, 655, 903
PpsI GAGTC 4 cut(s) 451, 570, 655, 903
PscI ACATGT 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 598, 886
PspGI CCWGG 2 cut(s) 598, 886
PspN4I GGNNCC 3 cut(s) 744, 745, 936
PspOMI GGGCCC 1 cut(s) 742
PspPI GGNCC 5 cut(s) 86, 97, 718, 742, 743
PspXI VCTCGAGB 1 cut(s) 540
PsuI RGATCY 1 cut(s) 934
PsyI GACNNNGTC 1 cut(s) 986
RsaI GTAC 3 cut(s) 290, 296, 308
RsaNI GTAC 3 cut(s) 289, 295, 307
RseI CAYNNNNRTG 3 cut(s) 252, 258, 827
SalI GTCGAC 1 cut(s) 709
SaqAI TTAA 4 cut(s) 171, 356, 569, 912
SatI GCNGC 5 cut(s) 537, 641, 776, 779, 948
Sau3AI GATC 3 cut(s) 70, 934, 975
Sau96I GGNCC 5 cut(s) 86, 97, 718, 742, 743
SchI GAGTC 4 cut(s) 452, 571, 656, 904
ScrFI CCNGG 3 cut(s) 215, 600, 888
SduI GDGCHC 2 cut(s) 375, 746
SfaNI GCATC 2 cut(s) 351, 761
Sfr274I CTCGAG 1 cut(s) 540
SfuI TTCGAA 1 cut(s) 227
SinI GGWCC 3 cut(s) 86, 97, 718
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 3 cut(s) 252, 258, 827
SmlI CTYRAG 2 cut(s) 355, 540
SmoI CTYRAG 2 cut(s) 355, 540
Sse9I AATT 4 cut(s) 134, 192, 531, 966
SsiI CCGC 8 cut(s) 89, 281, 384, 537, 578, 614, 779, 923
SspMI CTAG 3 cut(s) 461, 575, 665
StyD4I CCNGG 3 cut(s) 213, 598, 886
StyI CCWWGG 2 cut(s) 253, 714
TaaI ACNGT 2 cut(s) 377, 485
TaiI ACGT 2 cut(s) 80, 171
TaqI TCGA 6 cut(s) 24, 227, 396, 541, 610, 710
TaqII GACCGA 1 cut(s) 337
TasI AATT 4 cut(s) 134, 192, 531, 966
TatI WGTACW 2 cut(s) 294, 306
TauI GCSGC 2 cut(s) 539, 781
TfiI GAWTC 2 cut(s) 221, 586
Tru1I TTAA 4 cut(s) 171, 356, 569, 912
Tru9I TTAA 4 cut(s) 171, 356, 569, 912
TscAI CASTG 3 cut(s) 702, 708, 829
TseFI GTSAC 1 cut(s) 648
TseI GCWGC 3 cut(s) 640, 775, 947
Tsp45I GTSAC 1 cut(s) 648
TspDTI ATGAA 5 cut(s) 94, 127, 147, 543, 863
TspGWI ACGGA 2 cut(s) 418, 555
TspRI CASTG 3 cut(s) 702, 708, 829
Tth111I GACNNNGTC 1 cut(s) 986
Vha464I CTTAAG 1 cut(s) 355
VpaK11BI GGWCC 3 cut(s) 86, 97, 718
XapI RAATTY 2 cut(s) 134, 192
XceI RCATGY 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 540
XmiI GTMKAC 2 cut(s) 351, 710
XspI CTAG 3 cut(s) 461, 575, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.