MD02G1276600.v1.1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000918
Physical Location & Seq
Reverse (-)
33179051 .. 33180052
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1276600.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGGTCTGCCAGAAGGTTCTTGTTGATGAGGTGTCTGGTTGGCTGAGAGTGTTCGCCGATGGCTCAGTTGATCGGACATGGACTGGACCGCCTCAAGTCCAGTTCATGACCGAGCCAGTCGCACCACATGATGAATTCATCGATGGAGTTGCCACCCGAGATGTTTGCGTCAACGAAAACCTACGGCTACGAATTTATCTACCGGAGACAAACCCCGATGATTCTTTGAAGCTCCCTGTAATCCTTCATCTCCACGGAGGTGGCTTTTGTATAAGCCAGGCTGATTGGTACATGTACTACCAAATGTACACCAAGCTCGTACGCTCAGCCAAAGCCATTTGTATCTCTGTCTACTTAAGGCTCGCACCCGAGCACCGTCTCCCCGCTCCAATCATCGACGGCTTCTATGCGCTGCTCTGGCTTCGTTCCGTTGCACAAGGTGAATCATACGAGCAGTGGCTAGTCAGTCACGCAGACTTCAACCGTGTCTTTCTCATAGGGGACAGCTCGGGTGGAAATCTCGTACATGAAGTTGCCGCTCGAGCAGGGAAGGTGGATTTGCGTCCGTTGAGGCTAGCAGGTGGGATCCCTATCCACCCTGGATTCGTTCGATCAGTGAGGAGTAGGTCGGAGTTGGAGCAGCCAGAGTCACCTATGCTAACCCTAGACATGGTGGACAAGTTTCTGAGCTTGGCTCTGCCATTGGGGTCGACTAAGGACCATCCGATAACTTGTCCGATGGGCTCCAGAGCACCGTCTTTAGATACTCTGAAGCTGCCGCCGTTTCTTCTATGCATTGCTGAGATGGACATGATAGTAGACACAGAGATGGAATATTATGACGCCATGAAGAGGGCTAAGAAGGACGTGGAGCTTCTGATTAGTCCAGGGATGAGTCATAGCTTTTATCTTAACAAGATTGCGGTGGATATGGATCCTCAAACGGCTGAACAAACTGAGGCTCTAATTTCTGGGATAAAAAATTTCGTCAACAACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

334

Amino Acids

37.3

Weight (kDa)

5.47

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 64 - 177 3e-09 BD-FAE
Abhydrolase_3 PF07859 81 - 303 1e-47 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 569
Acc36I ACCTGC 1 cut(s) 569
AccBSI CCGCTC 2 cut(s) 386, 539
AccI GTMKAC 3 cut(s) 351, 710, 819
AciI CCGC 5 cut(s) 89, 384, 537, 779, 923
AclWI GGATC 4 cut(s) 580, 593, 929, 942
AcsI RAATTY 3 cut(s) 134, 192, 982
AcuI CTGAAG 1 cut(s) 791
AcyI GRCGYC 1 cut(s) 843
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 5 cut(s) 290, 296, 308, 321, 525
AfiI CCNNNNNNNGG 2 cut(s) 670, 852
AflII CTTAAG 1 cut(s) 355
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 2 cut(s) 229, 481
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 3 cut(s) 276, 598, 886
AleI CACNNNNGTG 1 cut(s) 258
AluBI AGCT 7 cut(s) 232, 316, 507, 690, 775, 874, 903
AluI AGCT 7 cut(s) 232, 316, 507, 690, 775, 874, 903
Alw21I GWGCWC 2 cut(s) 375, 754
Alw26I GTCTC 2 cut(s) 200, 383
AlwI GGATC 4 cut(s) 580, 593, 929, 942
Ama87I CYCGRG 4 cut(s) 156, 368, 508, 540
ApeKI GCWGC 3 cut(s) 412, 640, 775
ApoI RAATTY 3 cut(s) 134, 192, 982
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 2 cut(s) 86, 718
AsuHPI GGTGA 2 cut(s) 452, 642
AsuNHI GCTAGC 1 cut(s) 574
AvaI CYCGRG 4 cut(s) 156, 368, 508, 540
AvaII GGWCC 2 cut(s) 86, 718
BamHI GGATCC 2 cut(s) 585, 934
BanII GRGCYC 1 cut(s) 746
Bbv12I GWGCWC 2 cut(s) 375, 754
BbvI GCAGC 3 cut(s) 399, 652, 762
BccI CCATC 6 cut(s) 53, 137, 729, 733, 799, 823
BceAI ACGGC 4 cut(s) 200, 415, 766, 960
BcgI CGANNNNNNTGC 4 cut(s) 131, 147, 165, 181
BciT130I CCWGG 3 cut(s) 278, 600, 888
BcoDI GTCTC 2 cut(s) 200, 383
BfaI CTAG 3 cut(s) 461, 575, 665
BfrI CTTAAG 1 cut(s) 355
BfuAI ACCTGC 1 cut(s) 569
BisI GCNGC 5 cut(s) 413, 537, 641, 776, 779
BlpI GCTNAGC 1 cut(s) 325
BlsI GCNGC 5 cut(s) 414, 538, 642, 777, 780
Bme1390I CCNGG 3 cut(s) 278, 600, 888
Bme18I GGWCC 2 cut(s) 86, 718
BmeT110I CYCGRG 4 cut(s) 156, 368, 508, 540
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 2 cut(s) 86, 718
BmiI GGNNCC 3 cut(s) 587, 745, 936
BmrFI CCNGG 3 cut(s) 278, 600, 888
BmtI GCTAGC 1 cut(s) 578
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpmI CTGGAG 1 cut(s) 730
Bpu1102I GCTNAGC 1 cut(s) 325
BpuEI CTTGAG 1 cut(s) 78
Bsa29I ATCGAT 1 cut(s) 141
BsaBI GATNNNNATC 2 cut(s) 590, 933
BsaHI GRCGYC 1 cut(s) 843
BsaJI CCNNGG 3 cut(s) 253, 598, 887
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 670, 852
Bse1I ACTGG 3 cut(s) 88, 100, 116
Bse3DI GCAATG 1 cut(s) 795
Bse8I GATNNNNATC 2 cut(s) 590, 933
BseBI CCWGG 3 cut(s) 278, 600, 888
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 253, 598, 887
BseGI GGATG 2 cut(s) 721, 897
BseJI GATNNNNATC 2 cut(s) 590, 933
BseLI CCNNNNNNNGG 2 cut(s) 670, 852
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 6 cut(s) 35, 78, 339, 677, 792, 948
BseNI ACTGG 3 cut(s) 88, 100, 116
BseRI GAGGAG 1 cut(s) 634
BseXI GCAGC 3 cut(s) 399, 652, 762
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 375, 754
BsiHKCI CYCGRG 4 cut(s) 156, 368, 508, 540
BsiSI CCGG 1 cut(s) 203
BsiWI CGTACG 1 cut(s) 319
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 670, 852
BsmAI GTCTC 2 cut(s) 200, 383
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 515
BsoBI CYCGRG 4 cut(s) 156, 368, 508, 540
Bsp1286I GDGCHC 3 cut(s) 375, 746, 754
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 70, 585, 611, 934
Bsp1720I GCTNAGC 1 cut(s) 325
BspACI CCGC 5 cut(s) 89, 384, 537, 779, 923
BspCNI CTCAG 6 cut(s) 36, 77, 338, 678, 793, 949
BspDI ATCGAT 1 cut(s) 141
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 3 cut(s) 587, 745, 936
BspMI ACCTGC 1 cut(s) 569
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 4 cut(s) 580, 593, 929, 942
BspTI CTTAAG 1 cut(s) 355
BsrBI CCGCTC 2 cut(s) 386, 539
BsrDI GCAATG 1 cut(s) 795
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 3 cut(s) 88, 100, 116
BssECI CCNNGG 3 cut(s) 253, 598, 887
BssMI GATC 4 cut(s) 70, 585, 611, 934
BssNI GRCGYC 1 cut(s) 843
Bst2UI CCWGG 3 cut(s) 278, 600, 888
Bst4CI ACNGT 3 cut(s) 377, 485, 756
Bst6I CTCTTC 1 cut(s) 845
BstACI GRCGYC 1 cut(s) 843
BstAFI CTTAAG 1 cut(s) 355
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 363, 576
BstDEI CTNAG 8 cut(s) 44, 64, 325, 686, 714, 801, 858, 957
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 2 cut(s) 721, 897
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 4 cut(s) 73, 588, 614, 937
BstMAI GTCTC 2 cut(s) 200, 383
BstMBI GATC 4 cut(s) 70, 585, 611, 934
BstMWI GCNNNNNNNGC 2 cut(s) 418, 542
BstNI CCWGG 3 cut(s) 278, 600, 888
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 3 cut(s) 276, 598, 886
BstV1I GCAGC 3 cut(s) 399, 652, 762
BstX2I RGATCY 2 cut(s) 585, 934
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 2 cut(s) 585, 934
Bsu15I ATCGAT 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 253
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 2 cut(s) 721, 897
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 2 cut(s) 461, 621
BveI ACCTGC 1 cut(s) 569
Cac8I GCNNGC 2 cut(s) 363, 576
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 86, 718
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 3 cut(s) 157, 551, 851
Csp6I GTAC 5 cut(s) 289, 295, 307, 320, 524
CviAII CATG 8 cut(s) 78, 106, 128, 292, 527, 670, 811, 847
CviQI GTAC 5 cut(s) 289, 295, 307, 320, 524
DdeI CTNAG 8 cut(s) 44, 64, 325, 686, 714, 801, 858, 957
DpnI GATC 4 cut(s) 72, 587, 613, 936
DpnII GATC 4 cut(s) 70, 585, 611, 934
DraIII CACNNNGTG 1 cut(s) 440
Eam1104I CTCTTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 845
Eco24I GRGCYC 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 86, 718
Eco57I CTGAAG 1 cut(s) 791
Eco88I CYCGRG 4 cut(s) 156, 368, 508, 540
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 3 cut(s) 276, 598, 886
EcoT22I ATGCAT 1 cut(s) 797
EcoT38I GRGCYC 1 cut(s) 746
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 8 cut(s) 81, 109, 131, 295, 530, 673, 814, 850
FaqI GGGAC 1 cut(s) 515
FatI CATG 8 cut(s) 77, 105, 127, 291, 526, 669, 810, 846
FauI CCCGC 1 cut(s) 391
FblI GTMKAC 3 cut(s) 351, 710, 819
Fnu4HI GCNGC 5 cut(s) 413, 537, 641, 776, 779
FokI GGATG 2 cut(s) 708, 904
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 5 cut(s) 413, 537, 641, 776, 779
FspBI CTAG 3 cut(s) 461, 575, 665
GlaI GCGC 1 cut(s) 411
GluI GCNGC 5 cut(s) 413, 537, 641, 776, 779
GsuI CTGGAG 1 cut(s) 730
HapII CCGG 1 cut(s) 203
HgaI GACGC 3 cut(s) 157, 551, 851
HhaI GCGC 1 cut(s) 412
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 8 cut(s) 81, 109, 131, 295, 530, 673, 814, 850
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HincII GTYRAC 3 cut(s) 172, 711, 991
HindII GTYRAC 3 cut(s) 172, 711, 991
HinfI GANTC 5 cut(s) 221, 443, 603, 647, 895
HpaII CCGG 1 cut(s) 203
HphI GGTGA 2 cut(s) 452, 642
Hpy166II GTNNAC 7 cut(s) 172, 309, 352, 676, 711, 820, 991
Hpy188I TCNGA 7 cut(s) 75, 631, 687, 726, 738, 771, 879
Hpy188III TCNNGA 2 cut(s) 106, 747
Hpy8I GTNNAC 7 cut(s) 172, 309, 352, 676, 711, 820, 991
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 4 cut(s) 7, 254, 544, 856
HpyCH4III ACNGT 3 cut(s) 377, 485, 756
HpyCH4IV ACGT 1 cut(s) 867
HpyCH4V TGCA 2 cut(s) 434, 795
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 542
HpyF3I CTNAG 8 cut(s) 44, 64, 325, 686, 714, 801, 858, 957
HpySE526I ACGT 1 cut(s) 867
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 8 cut(s) 81, 109, 131, 295, 530, 673, 814, 850
HspAI GCGC 1 cut(s) 410
Kzo9I GATC 4 cut(s) 70, 585, 611, 934
LmnI GCTCC 5 cut(s) 237, 391, 637, 749, 871
Lsp1109I GCAGC 3 cut(s) 399, 652, 762
MaeI CTAG 3 cut(s) 461, 575, 665
MaeII ACGT 1 cut(s) 867
MaeIII GTNAC 2 cut(s) 467, 648
MalI GATC 4 cut(s) 72, 587, 613, 936
MbiI CCGCTC 2 cut(s) 386, 539
MboI GATC 4 cut(s) 70, 585, 611, 934
MboII GAAGA 2 cut(s) 779, 862
MflI RGATCY 2 cut(s) 585, 934
MhlI GDGCHC 3 cut(s) 375, 746, 754
MluCI AATT 4 cut(s) 134, 192, 966, 982
MlyI GAGTC 2 cut(s) 656, 904
MmeI TCCRAC 2 cut(s) 609, 615
MnlI CCTC 8 cut(s) 22, 102, 251, 564, 612, 846, 948, 952
Mph1103I ATGCAT 1 cut(s) 797
MseI TTAA 2 cut(s) 356, 912
MslI CAYNNNNRTG 2 cut(s) 258, 827
MspCI CTTAAG 1 cut(s) 355
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 3 cut(s) 278, 600, 888
MvaI CCWGG 3 cut(s) 278, 600, 888
MwoI GCNNNNNNNGC 2 cut(s) 418, 542
NdeII GATC 4 cut(s) 70, 585, 611, 934
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 8 cut(s) 81, 109, 131, 295, 530, 673, 814, 850
NlaIV GGNNCC 3 cut(s) 587, 745, 936
NmuCI GTSAC 2 cut(s) 467, 648
NsiI ATGCAT 1 cut(s) 797
NspI RCATGY 1 cut(s) 295
OliI CACNNNNGTG 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 540
PagI TCATGA 1 cut(s) 105
PaqCI CACCTGC 1 cut(s) 569
PciI ACATGT 1 cut(s) 291
PfeI GAWTC 3 cut(s) 221, 443, 603
Pfl23II CGTACG 1 cut(s) 319
PkrI GCNGC 5 cut(s) 414, 538, 642, 777, 780
PleI GAGTC 2 cut(s) 655, 903
PpsI GAGTC 2 cut(s) 655, 903
PscI ACATGT 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 276, 598, 886
PspGI CCWGG 3 cut(s) 276, 598, 886
PspLI CGTACG 1 cut(s) 319
PspN4I GGNNCC 3 cut(s) 587, 745, 936
PspPI GGNCC 2 cut(s) 86, 718
PspXI VCTCGAGB 1 cut(s) 540
PsuI RGATCY 2 cut(s) 585, 934
RsaI GTAC 5 cut(s) 290, 296, 308, 321, 525
RsaNI GTAC 5 cut(s) 289, 295, 307, 320, 524
RseI CAYNNNNRTG 2 cut(s) 258, 827
SalI GTCGAC 1 cut(s) 709
SaqAI TTAA 2 cut(s) 356, 912
SatI GCNGC 5 cut(s) 413, 537, 641, 776, 779
Sau3AI GATC 4 cut(s) 70, 585, 611, 934
Sau96I GGNCC 2 cut(s) 86, 718
SchI GAGTC 2 cut(s) 656, 904
ScrFI CCNGG 3 cut(s) 278, 600, 888
SduI GDGCHC 3 cut(s) 375, 746, 754
Sfr274I CTCGAG 1 cut(s) 540
SinI GGWCC 2 cut(s) 86, 718
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 2 cut(s) 258, 827
SmlI CTYRAG 3 cut(s) 93, 355, 540
SmoI CTYRAG 3 cut(s) 93, 355, 540
Sse9I AATT 4 cut(s) 134, 192, 966, 982
SsiI CCGC 5 cut(s) 89, 384, 537, 779, 923
SspI AATATT 1 cut(s) 836
SspMI CTAG 3 cut(s) 461, 575, 665
StyD4I CCNGG 3 cut(s) 276, 598, 886
TaaI ACNGT 3 cut(s) 377, 485, 756
TaiI ACGT 1 cut(s) 870
TaqI TCGA 5 cut(s) 141, 396, 541, 610, 710
TaqII GACCGA 1 cut(s) 125
TasI AATT 4 cut(s) 134, 192, 966, 982
TatI WGTACW 2 cut(s) 294, 306
TauI GCSGC 2 cut(s) 539, 781
TfiI GAWTC 3 cut(s) 221, 443, 603
Tru1I TTAA 2 cut(s) 356, 912
Tru9I TTAA 2 cut(s) 356, 912
TscAI CASTG 2 cut(s) 461, 621
TseFI GTSAC 2 cut(s) 467, 648
TseI GCWGC 3 cut(s) 412, 640, 775
Tsp45I GTSAC 2 cut(s) 467, 648
TspDTI ATGAA 6 cut(s) 94, 127, 147, 236, 543, 863
TspGWI ACGGA 3 cut(s) 270, 418, 555
TspRI CASTG 2 cut(s) 461, 621
Vha464I CTTAAG 1 cut(s) 355
VpaK11BI GGWCC 2 cut(s) 86, 718
XapI RAATTY 3 cut(s) 134, 192, 982
XceI RCATGY 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 540
XmiI GTMKAC 3 cut(s) 351, 710, 819
XspI CTAG 3 cut(s) 461, 575, 665
Zsp2I ATGCAT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.