Rh1DG092600

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000918
Physical Location & Seq
Forward (+)
17370493 .. 17371841
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG092600.1

Sequence Viewer

Length: 1017 bp
ATGGTCCGCGAGAAGAAGCTCGTCGATGAAGTTTCCGGTTGGCTTAGACTCTACGACGATGGCTCAGTGGACCGGACATGGACTGGACCACCTCAGGTGCAGTTCATGGCCGAGCCCGTCCCACCACACCATGAATTCATCAACGGAGTTGCCACCAAAGATGTCATGGTGGATGAGATGTCCGGCCTTCGCGTCCGAATATATGTTCCGGAGAGAAAGCCGGAGCAGGACGATGACGAGACTAAGCTACCGGTAATCCTTCATTTCCATGGGGGCGGTTTTTGCATTAGCCAAGCCGATTGGTTCATGTACTACCACATGTACGCTAACCTCGCACGTGCAGCCAACGCCATTTGCGTCTCCGTCTATCTACGCCTTGCACCCGAGCACCGTCTCCCAGCTCCGATCAACGACGGCTGCTCGGCCCTCCTCTGGCTCCGCTCCTTGGCTCGAGCAGAGTCATACGAGTCGTGGCTGAGTGACGATGCTGACTTTTCCCGAGTTTTTCTCATAGGAGACAGCTCAGGTGGCAACATAGTCCATGAAGTGGCCGCTCGAGCTGGGAGGCTTGATTTGAGTCCCTTGAAGCTTGCCGGAGGGATTCCGATCCACCCGGGTTTCGTTCGAGCCGTGAGGAGCCAGTCGGAGCTGGAGCAGCCCGAGTCGCCGTTACTAACCCTAGACATGGTGGACAAGTTCTTGAGCTTGTCACTGCCGGTGGGATCCACCAAGGATCATCCGATTACGTGTCCGATGGGTTCTAGGGCCCCGGCGCTGGATAGCTTGAAGCTGCCGCCGTTCCTTCTGTGCGTTGCGGAGAAGGACATGCTTGTGGACACGGAGATGGAGTACTTTGAGGCCATGAAGAAGGCGAAGAAGGATGTCGAGCTTTTGATCAGCCCTGGAATGAGTCATAGTTTTTATCTTAACAAGATTGCAATAGATATGGACCCGCAAACGAGTGAGCAAACTGAGAGTCTGATTTCTGGGATAGTAGAGTTCGTCAAGAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

338

Amino Acids

37.72

Weight (kDa)

5.24

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 78 - 181 6.1e-09 BD-FAE
Abhydrolase_3 PF07859 86 - 308 5.4e-51 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 2 cut(s) 441, 554
AccII CGCG 2 cut(s) 9, 192
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 7 cut(s) 7, 276, 439, 552, 794, 815, 953
AclWI GGATC 4 cut(s) 601, 717, 730, 741
AcoI YGGCCR 2 cut(s) 108, 549
AcsI RAATTY 1 cut(s) 134
AcvI CACGTG 1 cut(s) 338
AfaI GTAC 3 cut(s) 311, 323, 851
AfiI CCNNNNNNNGG 5 cut(s) 432, 445, 547, 685, 775
AflIII ACRYGT 2 cut(s) 318, 746
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 2 cut(s) 586, 787
AjnI CCWGG 1 cut(s) 901
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 4 cut(s) 233, 364, 398, 510
AlwI GGATC 4 cut(s) 601, 717, 730, 741
Ama87I CYCGRG 6 cut(s) 383, 450, 498, 555, 613, 659
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 6 cut(s) 108, 184, 423, 549, 765, 858
ApaI GGGCCC 1 cut(s) 769
ApeKI GCWGC 4 cut(s) 341, 417, 655, 790
ApoI RAATTY 1 cut(s) 134
ArsI GACNNNNNNTTYG 2 cut(s) 961, 993
AsiGI ACCGGT 1 cut(s) 250
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 7 cut(s) 4, 70, 86, 424, 765, 766, 949
AsuC2I CCSGG 3 cut(s) 614, 615, 770
AvaI CYCGRG 6 cut(s) 383, 450, 498, 555, 613, 659
AvaII GGWCC 4 cut(s) 4, 70, 86, 949
AxyI CCTNAGG 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 769
BamHI GGATCC 1 cut(s) 722
BanII GRGCYC 2 cut(s) 117, 769
BbrPI CACGTG 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 4 cut(s) 353, 404, 667, 777
BccI CCATC 3 cut(s) 53, 748, 838
BceAI ACGGC 4 cut(s) 430, 614, 652, 781
BciT130I CCWGG 1 cut(s) 903
BclI TGATCA 1 cut(s) 894
BcnI CCSGG 3 cut(s) 614, 615, 770
BcoDI GTCTC 4 cut(s) 233, 364, 398, 510
BfaI CTAG 2 cut(s) 680, 762
BfoI RGCGCY 1 cut(s) 776
BisI GCNGC 6 cut(s) 342, 418, 552, 656, 791, 794
BlsI GCNGC 6 cut(s) 343, 419, 553, 657, 792, 795
BmcAI AGTACT 1 cut(s) 851
Bme1390I CCNGG 4 cut(s) 614, 615, 770, 903
Bme18I GGWCC 4 cut(s) 4, 70, 86, 949
BmeT110I CYCGRG 6 cut(s) 383, 450, 498, 555, 613, 659
BmgT120I GGNCC 7 cut(s) 4, 70, 86, 424, 765, 766, 949
BmiI GGNNCC 6 cut(s) 437, 638, 724, 767, 768, 951
BmrFI CCNGG 4 cut(s) 614, 615, 770, 903
BmsI GCATC 1 cut(s) 475
BplI GAGNNNNNCTC 2 cut(s) 492, 524
BpmI CTGGAG 1 cut(s) 671
Bpu10I CCTNAGC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 721
BpuMI CCSGG 3 cut(s) 614, 615, 770
BsaAI YACGTR 2 cut(s) 338, 747
BsaBI GATNNNNATC 1 cut(s) 605
BsaJI CCNNGG 6 cut(s) 268, 444, 613, 729, 768, 901
BsaWI WCCGGW 4 cut(s) 35, 72, 208, 250
BsaXI ACNNNNNCTCC 2 cut(s) 833, 863
Bsc4I CCNNNNNNNGG 5 cut(s) 432, 445, 547, 685, 775
Bse118I RCCGGY 2 cut(s) 250, 715
Bse1I ACTGG 2 cut(s) 88, 640
Bse21I CCTNAGG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 605
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 1 cut(s) 903
BseDI CCNNGG 6 cut(s) 268, 444, 613, 729, 768, 901
BseGI GGATG 3 cut(s) 178, 736, 886
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 5 cut(s) 432, 445, 547, 685, 775
BseMII CTCAG 5 cut(s) 78, 107, 467, 537, 963
BseNI ACTGG 2 cut(s) 88, 640
BseRI GAGGAG 2 cut(s) 419, 649
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 4 cut(s) 353, 404, 667, 777
BseYI CCCAGC 2 cut(s) 397, 560
BsgI GTGCAG 2 cut(s) 119, 360
Bsh1236I CGCG 2 cut(s) 9, 192
BshFI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BshTI ACCGGT 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 6 cut(s) 383, 450, 498, 555, 613, 659
BslFI GGGAC 2 cut(s) 104, 564
BslI CCNNNNNNNGG 5 cut(s) 432, 445, 547, 685, 775
BsmAI GTCTC 4 cut(s) 233, 364, 398, 510
BsmBI CGTCTC 2 cut(s) 364, 398
BsmFI GGGAC 2 cut(s) 104, 564
BsnI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BsoBI CYCGRG 6 cut(s) 383, 450, 498, 555, 613, 659
Bsp120I GGGCCC 1 cut(s) 765
Bsp1286I GDGCHC 3 cut(s) 117, 390, 769
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 5 cut(s) 405, 606, 722, 733, 894
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 7 cut(s) 7, 276, 439, 552, 794, 815, 953
BspANI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BspCNI CTCAG 5 cut(s) 77, 106, 468, 536, 964
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 2 cut(s) 9, 192
BspLI GGNNCC 6 cut(s) 437, 638, 724, 767, 768, 951
BspPI GGATC 4 cut(s) 601, 717, 730, 741
BsrBI CCGCTC 2 cut(s) 441, 554
BsrFI RCCGGY 2 cut(s) 250, 715
BsrI ACTGG 2 cut(s) 88, 640
BssAI RCCGGY 2 cut(s) 250, 715
BssECI CCNNGG 6 cut(s) 268, 444, 613, 729, 768, 901
BssMI GATC 5 cut(s) 405, 606, 722, 733, 894
BssT1I CCWWGG 3 cut(s) 268, 444, 729
Bst2UI CCWGG 1 cut(s) 903
Bst4CI ACNGT 1 cut(s) 392
BstBAI YACGTR 2 cut(s) 338, 747
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 7 cut(s) 44, 64, 93, 243, 476, 523, 972
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 178, 736, 886
BstFNI CGCG 2 cut(s) 9, 192
BstH2I RGCGCY 1 cut(s) 776
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 5 cut(s) 408, 609, 725, 736, 897
BstMAI GTCTC 4 cut(s) 233, 364, 398, 510
BstMBI GATC 5 cut(s) 405, 606, 722, 733, 894
BstMWI GCNNNNNNNGC 8 cut(s) 282, 332, 341, 347, 528, 557, 655, 664
BstNI CCWGG 1 cut(s) 903
BstNSI RCATGY 2 cut(s) 322, 829
BstSCI CCNGG 4 cut(s) 612, 613, 768, 901
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 2 cut(s) 9, 192
BstV1I GCAGC 4 cut(s) 353, 404, 667, 777
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
Bsu36I CCTNAGG 1 cut(s) 93
BsuRI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 3 cut(s) 178, 736, 886
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 3 cut(s) 72, 710, 1012
Cac8I GCNNGC 1 cut(s) 591
CfoI GCGC 1 cut(s) 775
Cfr10I RCCGGY 2 cut(s) 250, 715
Cfr13I GGNCC 7 cut(s) 4, 70, 86, 424, 765, 766, 949
Cfr9I CCCGGG 1 cut(s) 613
CseI GACGC 2 cut(s) 181, 346
Csp6I GTAC 3 cut(s) 310, 322, 850
CspAI ACCGGT 1 cut(s) 250
CviQI GTAC 3 cut(s) 310, 322, 850
DdeI CTNAG 7 cut(s) 44, 64, 93, 243, 476, 523, 972
DpnI GATC 5 cut(s) 407, 608, 724, 735, 896
DpnII GATC 5 cut(s) 405, 606, 722, 733, 894
EaeI YGGCCR 2 cut(s) 108, 549
Eco130I CCWWGG 3 cut(s) 268, 444, 729
Eco24I GRGCYC 2 cut(s) 117, 769
Eco47I GGWCC 4 cut(s) 4, 70, 86, 949
Eco72I CACGTG 1 cut(s) 338
Eco81I CCTNAGG 1 cut(s) 93
Eco88I CYCGRG 6 cut(s) 383, 450, 498, 555, 613, 659
EcoO109I RGGNCCY 2 cut(s) 765, 766
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 901
EcoT14I CCWWGG 3 cut(s) 268, 444, 729
EcoT38I GRGCYC 2 cut(s) 117, 769
ErhI CCWWGG 3 cut(s) 268, 444, 729
Esp3I CGTCTC 2 cut(s) 364, 398
FaqI GGGAC 2 cut(s) 104, 564
FauI CCCGC 1 cut(s) 960
FbaI TGATCA 1 cut(s) 894
Fnu4HI GCNGC 6 cut(s) 342, 418, 552, 656, 791, 794
FokI GGATG 3 cut(s) 185, 723, 893
FriOI GRGCYC 2 cut(s) 117, 769
Fsp4HI GCNGC 6 cut(s) 342, 418, 552, 656, 791, 794
FspBI CTAG 2 cut(s) 680, 762
GlaI GCGC 1 cut(s) 774
GluI GCNGC 6 cut(s) 342, 418, 552, 656, 791, 794
GsaI CCCAGC 2 cut(s) 401, 564
GsuI CTGGAG 1 cut(s) 671
HaeII RGCGCY 1 cut(s) 776
HaeIII GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
HgaI GACGC 2 cut(s) 181, 346
HhaI GCGC 1 cut(s) 775
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 8 cut(s) 48, 458, 467, 577, 601, 662, 910, 976
Hpy166II GTNNAC 3 cut(s) 70, 691, 835
Hpy188I TCNGA 7 cut(s) 197, 405, 606, 646, 741, 753, 981
Hpy188III TCNNGA 4 cut(s) 209, 498, 700, 1006
Hpy8I GTNNAC 3 cut(s) 70, 691, 835
Hpy99I CGWCG 3 cut(s) 26, 59, 416
HpyAV CCTTC 6 cut(s) 197, 269, 812, 814, 862, 871
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 2 cut(s) 337, 746
HpyCH4V TGCA 5 cut(s) 100, 285, 341, 380, 938
HpyF10VI GCNNNNNNNGC 8 cut(s) 282, 332, 341, 347, 528, 557, 655, 664
HpyF3I CTNAG 7 cut(s) 44, 64, 93, 243, 476, 523, 972
HpySE526I ACGT 2 cut(s) 337, 746
HspAI GCGC 1 cut(s) 773
Kpn2I TCCGGA 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 894
Kzo9I GATC 5 cut(s) 405, 606, 722, 733, 894
LmnI GCTCC 7 cut(s) 223, 406, 441, 446, 636, 646, 652
Lsp1109I GCAGC 4 cut(s) 353, 404, 667, 777
LweI GCATC 1 cut(s) 475
MaeI CTAG 2 cut(s) 680, 762
MaeII ACGT 2 cut(s) 337, 746
MaeIII GTNAC 3 cut(s) 479, 669, 708
MalI GATC 5 cut(s) 407, 608, 724, 735, 896
MbiI CCGCTC 2 cut(s) 441, 554
MboI GATC 5 cut(s) 405, 606, 722, 733, 894
MboII GAAGA 3 cut(s) 25, 877, 886
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 3 cut(s) 117, 390, 769
MluCI AATT 1 cut(s) 134
MlyI GAGTC 7 cut(s) 42, 467, 476, 586, 671, 919, 985
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 8 cut(s) 102, 341, 437, 440, 558, 590, 627, 850
MroI TCCGGA 1 cut(s) 208
MseI TTAA 1 cut(s) 927
MslI CAYNNNNRTG 3 cut(s) 267, 830, 842
MspR9I CCNGG 4 cut(s) 614, 615, 770, 903
MvaI CCWGG 1 cut(s) 903
MvnI CGCG 2 cut(s) 9, 192
MwoI GCNNNNNNNGC 8 cut(s) 282, 332, 341, 347, 528, 557, 655, 664
NciI CCSGG 3 cut(s) 614, 615, 770
NcoI CCATGG 1 cut(s) 268
NdeII GATC 5 cut(s) 405, 606, 722, 733, 894
NlaIV GGNNCC 6 cut(s) 437, 638, 724, 767, 768, 951
NmeAIII GCCGAG 2 cut(s) 136, 401
NmuCI GTSAC 2 cut(s) 479, 708
NspI RCATGY 2 cut(s) 322, 829
PaeR7I CTCGAG 2 cut(s) 450, 555
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 354, 627
PfeI GAWTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 547
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 6 cut(s) 343, 419, 553, 657, 792, 795
PleI GAGTC 7 cut(s) 42, 466, 475, 585, 670, 918, 984
PmaCI CACGTG 1 cut(s) 338
PmlI CACGTG 1 cut(s) 338
PpsI GAGTC 7 cut(s) 42, 466, 475, 585, 670, 918, 984
Ppu21I YACGTR 2 cut(s) 338, 747
PscI ACATGT 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 901
PspCI CACGTG 1 cut(s) 338
PspFI CCCAGC 2 cut(s) 397, 560
PspGI CCWGG 1 cut(s) 901
PspN4I GGNNCC 6 cut(s) 437, 638, 724, 767, 768, 951
PspOMI GGGCCC 1 cut(s) 765
PspPI GGNCC 7 cut(s) 4, 70, 86, 424, 765, 766, 949
PspXI VCTCGAGB 2 cut(s) 450, 555
PsuI RGATCY 1 cut(s) 722
RsaI GTAC 3 cut(s) 311, 323, 851
RsaNI GTAC 3 cut(s) 310, 322, 850
RseI CAYNNNNRTG 3 cut(s) 267, 830, 842
SaqAI TTAA 1 cut(s) 927
SatI GCNGC 6 cut(s) 342, 418, 552, 656, 791, 794
Sau3AI GATC 5 cut(s) 405, 606, 722, 733, 894
Sau96I GGNCC 7 cut(s) 4, 70, 86, 424, 765, 766, 949
ScaI AGTACT 1 cut(s) 851
SchI GAGTC 7 cut(s) 42, 467, 476, 586, 671, 919, 985
ScrFI CCNGG 4 cut(s) 614, 615, 770, 903
SduI GDGCHC 3 cut(s) 117, 390, 769
SfaNI GCATC 1 cut(s) 475
Sfr274I CTCGAG 2 cut(s) 450, 555
SinI GGWCC 4 cut(s) 4, 70, 86, 949
SlaI CTCGAG 2 cut(s) 450, 555
SmaI CCCGGG 1 cut(s) 615
SmiMI CAYNNNNRTG 3 cut(s) 267, 830, 842
SmlI CTYRAG 3 cut(s) 450, 555, 700
SmoI CTYRAG 3 cut(s) 450, 555, 700
Sse9I AATT 1 cut(s) 134
SsiI CCGC 7 cut(s) 7, 276, 439, 552, 794, 815, 953
SspMI CTAG 2 cut(s) 680, 762
StyD4I CCNGG 4 cut(s) 612, 613, 768, 901
StyI CCWWGG 3 cut(s) 268, 444, 729
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 2 cut(s) 340, 749
TaqI TCGA 5 cut(s) 24, 451, 556, 625, 885
TasI AATT 1 cut(s) 134
TatI WGTACW 2 cut(s) 309, 849
TauI GCSGC 2 cut(s) 554, 796
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 1 cut(s) 927
Tru9I TTAA 1 cut(s) 927
TscAI CASTG 2 cut(s) 72, 717
TseFI GTSAC 2 cut(s) 479, 708
TseI GCWGC 4 cut(s) 341, 417, 655, 790
Tsp45I GTSAC 2 cut(s) 479, 708
TspDTI ATGAA 8 cut(s) 42, 94, 127, 147, 251, 295, 558, 878
TspGWI ACGGA 3 cut(s) 159, 352, 854
TspMI CCCGGG 1 cut(s) 613
TspRI CASTG 2 cut(s) 72, 717
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 4 cut(s) 4, 70, 86, 949
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 322, 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XhoI CTCGAG 2 cut(s) 450, 555
XmaI CCCGGG 1 cut(s) 613
XspI CTAG 2 cut(s) 680, 762
ZrmI AGTACT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.