RLG00000029967

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000918
Physical Location & Seq
Reverse (-)
52388256 .. 52393291
5036 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029967

Sequence Viewer

Length: 1248 bp
ATGTCCTTCTCCCCCACCCCTAAAGCGTATCGATGTCCTTCTCCCCTAAATCGAATCAGCCGTATTAGAGTGGACGCTGCTGTGAAGAAGAAGGTTGAGGAGATAATGCCCATTGCCACCGGCCACGAGCTAGAGGAGCTTGCAGCTGAATTTGAGTCTCTGATTTGCATTTTTCAACTTTCTAACGGAAAGAATATTCTGGAAATTGACCATCCCGAAGGTTTTGCACCACTGGGCTTCTTAGTGATGTTGTTGTTGAAGTTGGAGAAATGTGATTCCATCTTCACAAGAGTATACGACGACGGCTCAGTCGACCGGACATGGACAGGACTCCCGGAGTTCACTTTCCTATTTCAGTCGGTTTCACCTCACGAGGAATTCATCAAAGGCGTTGCCACAAAAGACGTGGTCATTGACAAAACGTCCGGCCATCGTGTCCGAATATACCTACCGGAGAGAAAGCCGGAGGACAATGACGAGACTAAGCTACCCATAATCCTTCATTTCCATGGAGGGGGATTTTGCATTTCCGAAGCCGATTGGTACATGTACTACTACATCTACTCCAAGATCGCACGCGCGGCCAACGCTATTATTGTCTCCGTCTACCTAAGACAAGCCCCCGAGCACCGTCTTCCAGCTCAGATAGATGACAGCTTCAGTGCTCTCCTCTGGCTACGATCTGTGGCTCAAGCAGATTCGTATGAACCGTGGTTAAATGACCATGCAGACTTGAACCGCATTTTCCTCATAGGGGACAGTTCAGGAGGAAACATTGTACATGAAGTGGCTGCTCGGGCAGGGAATGCGGATTTGGGTCAGTTCAAGTTATCCGGTGGGATTCTTATCCAACCTGGCTTTGTCCGGGTAACGAGGAGCAAGTCGGAGTTGGAACAACCGCAGACATCATTTTTTAACGTAGATATGGTGGACAAGTTGTTCAGCTTTTCACTTCCGGTGGGGTCAACGAAAGACCATCGGATCACATGTCCAATGGGTTCATTGGCCCCGCCACTGGACAGTCTAAAGCTGCCTCCGATGCTCATTTTCATTGGGGGGATCGACGTCTTTCAGGACACTGAGATGGAATACTATGAGGCCATGAAGAAGGCTAACCACGATGTGCAGCTCTTGACCATCCCCGGAATGAGACATGCGTTTTATCTCAACGCGGACATGGACCCACGCATGGCTGTCCCAACTGAAAGTCTAGTTTCTGGGGTGGTAGAGTTCGTCAAGAAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

416

Amino Acids

46.63

Weight (kDa)

5.57

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 157 - 266 2.8e-10 BD-FAE
Abhydrolase_3 PF07859 166 - 388 4.1e-49 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AatII GACGTC 1 cut(s) 1068
AccI GTMKAC 3 cut(s) 294, 312, 606
AccII CGCG 3 cut(s) 579, 581, 1172
AciI CCGC 6 cut(s) 581, 739, 809, 899, 1010, 1172
AclWI GGATC 2 cut(s) 989, 1067
AcoI YGGCCR 3 cut(s) 121, 427, 582
AcsI RAATTY 2 cut(s) 149, 377
AcuI CTGAAG 1 cut(s) 643
AcyI GRCGYC 1 cut(s) 1065
AdeI CACNNNGTG 1 cut(s) 1123
AfaI GTAC 3 cut(s) 545, 551, 780
AfiI CCNNNNNNNGG 4 cut(s) 514, 754, 1015, 1189
AflIII ACRYGT 2 cut(s) 546, 986
AgsI TTSAA 4 cut(s) 176, 259, 736, 826
AhdI GACNNNNNGTC 1 cut(s) 421
AjiI CACGTC 1 cut(s) 406
AjnI CCWGG 1 cut(s) 853
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AloI GAACNNNNNNTCC 2 cut(s) 923, 955
AluBI AGCT 9 cut(s) 130, 139, 146, 487, 641, 657, 945, 1030, 1129
AluI AGCT 9 cut(s) 130, 139, 146, 487, 641, 657, 945, 1030, 1129
Alw21I GWGCWC 2 cut(s) 630, 667
Alw26I GTCTC 4 cut(s) 162, 473, 604, 1144
AlwI GGATC 2 cut(s) 989, 1067
Ama87I CYCGRG 2 cut(s) 623, 795
AoxI GGCC 5 cut(s) 121, 427, 582, 1005, 1098
ApeKI GCWGC 5 cut(s) 77, 143, 791, 1030, 1126
ApoI RAATTY 2 cut(s) 149, 377
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 1006, 1180
AsuC2I CCSGG 3 cut(s) 335, 866, 1143
AsuHPI GGTGA 1 cut(s) 357
AvaI CYCGRG 2 cut(s) 623, 795
AvaII GGWCC 1 cut(s) 1180
BauI CACGAG 2 cut(s) 125, 371
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 2 cut(s) 630, 667
BbvI GCAGC 5 cut(s) 64, 155, 778, 1017, 1138
BccI CCATC 6 cut(s) 219, 287, 438, 984, 1078, 1145
BceAI ACGGC 2 cut(s) 45, 319
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 1 cut(s) 855
BcnI CCSGG 3 cut(s) 335, 866, 1143
BcoDI GTCTC 4 cut(s) 162, 473, 604, 1144
BfaI CTAG 2 cut(s) 131, 1211
BisI GCNGC 6 cut(s) 78, 144, 582, 792, 1031, 1127
BlsI GCNGC 6 cut(s) 79, 145, 583, 793, 1032, 1128
Bme1390I CCNGG 4 cut(s) 335, 855, 866, 1143
Bme18I GGWCC 1 cut(s) 1180
BmeRI GACNNNNNGTC 1 cut(s) 421
BmeT110I CYCGRG 2 cut(s) 623, 795
BmgBI CACGTC 1 cut(s) 406
BmgT120I GGNCC 2 cut(s) 1006, 1180
BmiI GGNNCC 2 cut(s) 1008, 1182
BmrFI CCNGG 4 cut(s) 335, 855, 866, 1143
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 1 cut(s) 1029
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 675
BpuMI CCSGG 3 cut(s) 335, 866, 1143
Bsa29I ATCGAT 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 845
BsaHI GRCGYC 1 cut(s) 1065
BsaJI CCNNGG 3 cut(s) 508, 710, 1141
BsaWI WCCGGW 4 cut(s) 315, 451, 833, 955
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 4 cut(s) 514, 754, 1015, 1189
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 2 cut(s) 237, 1020
Bse3DI GCAATG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 845
BseBI CCWGG 1 cut(s) 855
BseCI ATCGAT 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 508, 710, 1141
BseGI GGATG 2 cut(s) 211, 1137
BseJI GATNNNNATC 1 cut(s) 845
BseLI CCNNNNNNNGG 4 cut(s) 514, 754, 1015, 1189
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 321, 656, 1071
BseNI ACTGG 2 cut(s) 237, 1020
BseRI GAGGAG 4 cut(s) 113, 149, 659, 889
BseXI GCAGC 5 cut(s) 64, 155, 778, 1017, 1138
BsgI GTGCAG 1 cut(s) 1145
Bsh1236I CGCG 3 cut(s) 579, 581, 1172
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 5 cut(s) 123, 429, 584, 1007, 1100
BshVI ATCGAT 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 2 cut(s) 630, 667
BsiHKCI CYCGRG 2 cut(s) 623, 795
BslFI GGGAC 2 cut(s) 770, 1181
BslI CCNNNNNNNGG 4 cut(s) 514, 754, 1015, 1189
BsmAI GTCTC 4 cut(s) 162, 473, 604, 1144
BsmFI GGGAC 2 cut(s) 770, 1181
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 5 cut(s) 123, 429, 584, 1007, 1100
BsoBI CYCGRG 2 cut(s) 623, 795
Bsp1286I GDGCHC 2 cut(s) 630, 667
Bsp1407I TGTACA 1 cut(s) 778
Bsp143I GATC 4 cut(s) 570, 680, 981, 1059
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 6 cut(s) 581, 739, 809, 899, 1010, 1172
BspANI GGCC 5 cut(s) 123, 429, 584, 1007, 1100
BspCNI CTCAG 3 cut(s) 320, 655, 1072
BspDI ATCGAT 1 cut(s) 31
BspFNI CGCG 3 cut(s) 579, 581, 1172
BspLI GGNNCC 2 cut(s) 1008, 1182
BspPI GGATC 2 cut(s) 989, 1067
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 778
BsrI ACTGG 2 cut(s) 237, 1020
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 508, 710, 1141
BssMI GATC 4 cut(s) 570, 680, 981, 1059
BssNAI GTATAC 1 cut(s) 295
BssNI GRCGYC 1 cut(s) 1065
BssSI CACGAG 2 cut(s) 125, 371
BssT1I CCWWGG 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 295
Bst2BI CACGAG 2 cut(s) 125, 371
Bst2UI CCWGG 1 cut(s) 855
Bst4CI ACNGT 4 cut(s) 632, 711, 761, 1022
BstACI GRCGYC 1 cut(s) 1065
BstAPI GCANNNNNTGC 1 cut(s) 806
BstAUI TGTACA 1 cut(s) 778
BstC8I GCNNGC 2 cut(s) 141, 577
BstDEI CTNAG 6 cut(s) 241, 307, 483, 611, 642, 1080
BstDSI CCRYGG 2 cut(s) 508, 710
BstF5I GGATG 2 cut(s) 211, 1137
BstFNI CGCG 3 cut(s) 579, 581, 1172
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 4 cut(s) 573, 683, 984, 1062
BstMAI GTCTC 4 cut(s) 162, 473, 604, 1144
BstMBI GATC 4 cut(s) 570, 680, 981, 1059
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 6 cut(s) 136, 581, 587, 797, 806, 1039
BstNI CCWGG 1 cut(s) 855
BstNSI RCATGY 3 cut(s) 550, 990, 1157
BstSCI CCNGG 4 cut(s) 333, 853, 864, 1141
BstUI CGCG 3 cut(s) 579, 581, 1172
BstV1I GCAGC 5 cut(s) 64, 155, 778, 1017, 1138
BstV2I GAAGAC 1 cut(s) 626
BstZ17I GTATAC 1 cut(s) 295
Bsu15I ATCGAT 1 cut(s) 31
BsuRI GGCC 5 cut(s) 123, 429, 584, 1007, 1100
BsuTUI ATCGAT 1 cut(s) 31
BtgI CCRYGG 2 cut(s) 508, 710
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 2 cut(s) 211, 1137
BtsIMutI CAGTG 4 cut(s) 230, 667, 1013, 1077
Cac8I GCNNGC 2 cut(s) 141, 577
CfoI GCGC 1 cut(s) 581
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 1006, 1180
ClaI ATCGAT 1 cut(s) 31
CseI GACGC 1 cut(s) 83
Csp6I GTAC 3 cut(s) 544, 550, 779
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviQI GTAC 3 cut(s) 544, 550, 779
DdeI CTNAG 6 cut(s) 241, 307, 483, 611, 642, 1080
DpnI GATC 4 cut(s) 572, 682, 983, 1061
DpnII GATC 4 cut(s) 570, 680, 981, 1059
DraIII CACNNNGTG 1 cut(s) 1123
DrdI GACNNNNNNGTC 1 cut(s) 308
DriI GACNNNNNGTC 1 cut(s) 421
DseDI GACNNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 3 cut(s) 121, 427, 582
Eam1105I GACNNNNNGTC 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 1180
Eco57I CTGAAG 1 cut(s) 643
Eco88I CYCGRG 2 cut(s) 623, 795
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
FaqI GGGAC 2 cut(s) 770, 1181
FauI CCCGC 1 cut(s) 1017
FblI GTMKAC 3 cut(s) 294, 312, 606
Fnu4HI GCNGC 6 cut(s) 78, 144, 582, 792, 1031, 1127
FokI GGATG 2 cut(s) 198, 1124
Fsp4HI GCNGC 6 cut(s) 78, 144, 582, 792, 1031, 1127
FspBI CTAG 2 cut(s) 131, 1211
GlaI GCGC 1 cut(s) 580
GluI GCNGC 6 cut(s) 78, 144, 582, 792, 1031, 1127
HaeIII GGCC 5 cut(s) 123, 429, 584, 1007, 1100
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 581
Hin1I GRCGYC 1 cut(s) 1065
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HincII GTYRAC 2 cut(s) 313, 966
HindII GTYRAC 2 cut(s) 313, 966
HinfI GANTC 6 cut(s) 54, 155, 275, 330, 698, 841
HphI GGTGA 1 cut(s) 357
Hpy166II GTNNAC 7 cut(s) 73, 295, 313, 342, 607, 931, 966
Hpy188I TCNGA 7 cut(s) 162, 440, 532, 645, 886, 981, 1038
Hpy188III TCNNGA 7 cut(s) 200, 215, 371, 765, 1073, 1132, 1237
Hpy8I GTNNAC 7 cut(s) 73, 295, 313, 342, 607, 931, 966
Hpy99I CGWCG 3 cut(s) 302, 305, 1067
HpyAV CCTTC 6 cut(s) 16, 48, 85, 212, 509, 1102
HpyCH4III ACNGT 4 cut(s) 632, 711, 761, 1022
HpyCH4IV ACGT 4 cut(s) 405, 422, 918, 1065
HpyCH4V TGCA 6 cut(s) 143, 168, 227, 525, 728, 1126
HpyF10VI GCNNNNNNNGC 6 cut(s) 136, 581, 587, 797, 806, 1039
HpyF3I CTNAG 6 cut(s) 241, 307, 483, 611, 642, 1080
HpySE526I ACGT 4 cut(s) 405, 422, 918, 1065
Hsp92I GRCGYC 1 cut(s) 1065
HspAI GCGC 1 cut(s) 579
Kzo9I GATC 4 cut(s) 570, 680, 981, 1059
LmnI GCTCC 2 cut(s) 136, 876
Lsp1109I GCAGC 5 cut(s) 64, 155, 778, 1017, 1138
LweI GCATC 1 cut(s) 1029
MaeI CTAG 2 cut(s) 131, 1211
MaeII ACGT 4 cut(s) 405, 422, 918, 1065
MaeIII GTNAC 1 cut(s) 868
MalI GATC 4 cut(s) 572, 682, 983, 1061
MboI GATC 4 cut(s) 570, 680, 981, 1059
MboII GAAGA 5 cut(s) 97, 100, 274, 626, 1117
MhlI GDGCHC 2 cut(s) 630, 667
MluCI AATT 3 cut(s) 149, 204, 377
MlyI GAGTC 2 cut(s) 164, 324
MmeI TCCRAC 4 cut(s) 243, 864, 870, 874
MseI TTAA 2 cut(s) 716, 915
MslI CAYNNNNRTG 2 cut(s) 507, 1082
MspA1I CMGCKG 1 cut(s) 146
MspR9I CCNGG 4 cut(s) 335, 855, 866, 1143
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 1 cut(s) 855
MvnI CGCG 3 cut(s) 579, 581, 1172
MwoI GCNNNNNNNGC 6 cut(s) 136, 581, 587, 797, 806, 1039
NciI CCSGG 3 cut(s) 335, 866, 1143
NcoI CCATGG 1 cut(s) 508
NdeII GATC 4 cut(s) 570, 680, 981, 1059
NlaIV GGNNCC 2 cut(s) 1008, 1182
NspI RCATGY 3 cut(s) 550, 990, 1157
PciI ACATGT 2 cut(s) 546, 986
PcsI WCGNNNNNNNCGW 3 cut(s) 58, 309, 707
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 4 cut(s) 54, 275, 698, 841
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 6 cut(s) 79, 145, 583, 793, 1032, 1128
PleI GAGTC 2 cut(s) 163, 324
PpsI GAGTC 2 cut(s) 163, 324
PscI ACATGT 2 cut(s) 546, 986
Psp6I CCWGG 1 cut(s) 853
PspGI CCWGG 1 cut(s) 853
PspN4I GGNNCC 2 cut(s) 1008, 1182
PspPI GGNCC 2 cut(s) 1006, 1180
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 545, 551, 780
RsaNI GTAC 3 cut(s) 544, 550, 779
RseI CAYNNNNRTG 2 cut(s) 507, 1082
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 2 cut(s) 716, 915
SatI GCNGC 6 cut(s) 78, 144, 582, 792, 1031, 1127
Sau3AI GATC 4 cut(s) 570, 680, 981, 1059
Sau96I GGNCC 2 cut(s) 1006, 1180
SchI GAGTC 2 cut(s) 164, 324
ScrFI CCNGG 4 cut(s) 335, 855, 866, 1143
SduI GDGCHC 2 cut(s) 630, 667
SfaNI GCATC 1 cut(s) 1029
SinI GGWCC 1 cut(s) 1180
SmiMI CAYNNNNRTG 2 cut(s) 507, 1082
SmlI CTYRAG 1 cut(s) 690
SmoI CTYRAG 1 cut(s) 690
Sse9I AATT 3 cut(s) 149, 204, 377
SsiI CCGC 6 cut(s) 581, 739, 809, 899, 1010, 1172
SspI AATATT 1 cut(s) 196
SspMI CTAG 2 cut(s) 131, 1211
StyD4I CCNGG 4 cut(s) 333, 853, 864, 1141
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 4 cut(s) 632, 711, 761, 1022
TaiI ACGT 4 cut(s) 408, 425, 921, 1068
TaqI TCGA 4 cut(s) 31, 52, 312, 1062
TasI AATT 3 cut(s) 149, 204, 377
TatI WGTACW 2 cut(s) 549, 778
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 4 cut(s) 54, 275, 698, 841
Tru1I TTAA 2 cut(s) 716, 915
Tru9I TTAA 2 cut(s) 716, 915
TscAI CASTG 4 cut(s) 237, 667, 1020, 1084
TseI GCWGC 5 cut(s) 77, 143, 791, 1030, 1126
TspDTI ATGAA 7 cut(s) 370, 491, 720, 798, 990, 1039, 1118
TspGWI ACGGA 2 cut(s) 201, 592
TspRI CASTG 4 cut(s) 237, 667, 1020, 1084
Tth111I GACNNNGTC 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 1180
XapI RAATTY 2 cut(s) 149, 377
XceI RCATGY 3 cut(s) 550, 990, 1157
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmiI GTMKAC 3 cut(s) 294, 312, 606
XspI CTAG 2 cut(s) 131, 1211
ZraI GACGTC 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.