RLG00000029962

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000918
Physical Location & Seq
Reverse (-)
52266925 .. 52267941
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029962

Sequence Viewer

Length: 1017 bp
ATGGTCCGCGAGAAGAAGCTCGTCGATGAAGTTTCCGGTTGGCTTAGACTCTACGACGATGGCTCAGTGGACCGGAAATGGACTGGACCACCTCAGGTGCAGTTCATGGCCGAGCCCGTCCCACCACATGATGAATTCATCAACGGAGTTGCCACCAAAGATGTCATGGTGGATGAGAGGTCCGGCCTTCGCGTCCGAATATATGTACCGGAGAGAAAGCTGGAGCAGGACGATGACGAGACTAAGCTACCGGTAATCCTTCATTTCCATGGGGGCGGCTTTTGCATTAGCCAAGCCGATTGGTTCATGTACTACCACATGTACGCTAACCTCGCACGTGCAGCCAACGCCATTTGTGTATCCGTCTATCTACGCCTTGCACCGGAGCACCGTCTCCCGGCTCCGATCAACGACGGCTGCTCGGCCCTCCTCTGGCTCCGCTCCTTGGCTCAAGCAGAGTCATACGAGTCGTGGCTGAGTGACGATGCTGACTTTTCCCGCGTTTTTCTCATAGGAGATAGCTCTGGAGGCAACATAGTCCATGAAGTGGCCGCTCGAGCTGGGAGGCTTGATTTGAGTCCCTTGAAGCTTGCCGGAGGGATTCCGATCCACCCGGGTTTCGTTCGAGCCGTGAGGAGCCGGTCGGAGCTGGAGCAGCCCGAGTCGCCGTTACTAACCCTAGACATGGTGGACAAGTTCTTGAGCTTGTCACTGCCGGTGGGATCCACCAAGGATCATCCGATTACGTGTCCGATGGGTTCTGGGGCCCCGGCGCTGGATAGCTTGAAGCTGCCGCCGTTCCTTCTGTGCATTGCGGAGAAGGACATGATTGTGGACACGGAGATGGAGTACTTTGAGGCCATGAAGAAGGCGAAGAAGGATGTGGAGCTTTTGATCAGCCCCGGAATGAGTCATAGTTTTTATCTTAACAAGATCGCCATAGATATGGACCCGCAAACGAGTGAGCAAACTGAGTGTCTGATTTCTGGGATAGTAGAGTTCGTCAAGAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

339

Amino Acids

37.7

Weight (kDa)

5.18

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 78 - 181 4.3e-09 BD-FAE
Abhydrolase_3 PF07859 86 - 308 9e-50 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 2 cut(s) 441, 554
AccII CGCG 3 cut(s) 9, 192, 501
AciI CCGC 8 cut(s) 7, 276, 439, 499, 552, 794, 815, 953
AclWI GGATC 4 cut(s) 601, 717, 730, 741
AcoI YGGCCR 2 cut(s) 108, 549
AcsI RAATTY 1 cut(s) 134
AcvI CACGTG 1 cut(s) 338
AfaI GTAC 4 cut(s) 207, 311, 323, 851
AfiI CCNNNNNNNGG 7 cut(s) 382, 397, 432, 445, 547, 685, 775
AflIII ACRYGT 2 cut(s) 318, 746
AgeI ACCGGT 1 cut(s) 250
AgsI TTSAA 2 cut(s) 586, 787
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 2 cut(s) 233, 398
AlwI GGATC 4 cut(s) 601, 717, 730, 741
Ama87I CYCGRG 3 cut(s) 555, 613, 659
AoxI GGCC 6 cut(s) 108, 184, 423, 549, 765, 858
ApaI GGGCCC 1 cut(s) 769
ApeKI GCWGC 4 cut(s) 341, 417, 655, 790
ApoI RAATTY 1 cut(s) 134
ArsI GACNNNNNNTTYG 2 cut(s) 961, 993
AsiGI ACCGGT 1 cut(s) 250
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 8 cut(s) 4, 70, 86, 180, 424, 765, 766, 949
AsuC2I CCSGG 5 cut(s) 398, 614, 615, 770, 903
AvaI CYCGRG 3 cut(s) 555, 613, 659
AvaII GGWCC 5 cut(s) 4, 70, 86, 180, 949
AxyI CCTNAGG 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 769
BamHI GGATCC 1 cut(s) 722
BanII GRGCYC 2 cut(s) 117, 769
BbrPI CACGTG 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 4 cut(s) 353, 404, 667, 777
BccI CCATC 3 cut(s) 53, 748, 838
BceAI ACGGC 4 cut(s) 430, 614, 652, 781
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 894
BcnI CCSGG 5 cut(s) 398, 614, 615, 770, 903
BcoDI GTCTC 2 cut(s) 233, 398
BfaI CTAG 1 cut(s) 680
BfoI RGCGCY 1 cut(s) 776
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 7 cut(s) 277, 342, 418, 552, 656, 791, 794
BlsI GCNGC 7 cut(s) 278, 343, 419, 553, 657, 792, 795
BmcAI AGTACT 1 cut(s) 851
Bme1390I CCNGG 5 cut(s) 398, 614, 615, 770, 903
Bme18I GGWCC 5 cut(s) 4, 70, 86, 180, 949
BmeT110I CYCGRG 3 cut(s) 555, 613, 659
BmgT120I GGNCC 8 cut(s) 4, 70, 86, 180, 424, 765, 766, 949
BmiI GGNNCC 8 cut(s) 402, 437, 638, 724, 766, 767, 768, 951
BmrFI CCNGG 5 cut(s) 398, 614, 615, 770, 903
BmsI GCATC 1 cut(s) 475
BpmI CTGGAG 3 cut(s) 242, 546, 671
BpuEI CTTGAG 2 cut(s) 435, 721
BpuMI CCSGG 5 cut(s) 398, 614, 615, 770, 903
BsaAI YACGTR 2 cut(s) 338, 747
BsaBI GATNNNNATC 1 cut(s) 605
BsaJI CCNNGG 6 cut(s) 268, 444, 613, 729, 768, 901
BsaWI WCCGGW 5 cut(s) 35, 72, 208, 250, 382
BsaXI ACNNNNNCTCC 2 cut(s) 833, 863
Bsc4I CCNNNNNNNGG 7 cut(s) 382, 397, 432, 445, 547, 685, 775
Bse118I RCCGGY 3 cut(s) 250, 639, 715
Bse1I ACTGG 1 cut(s) 88
Bse21I CCTNAGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 810
Bse8I GATNNNNATC 1 cut(s) 605
BseDI CCNNGG 6 cut(s) 268, 444, 613, 729, 768, 901
BseGI GGATG 3 cut(s) 178, 736, 886
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 7 cut(s) 382, 397, 432, 445, 547, 685, 775
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 4 cut(s) 78, 107, 467, 963
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 2 cut(s) 419, 649
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 4 cut(s) 353, 404, 667, 777
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 2 cut(s) 119, 360
Bsh1236I CGCG 3 cut(s) 9, 192, 501
Bsh1285I CGRYCG 1 cut(s) 644
BshFI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BshTI ACCGGT 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 3 cut(s) 555, 613, 659
BslFI GGGAC 2 cut(s) 104, 564
BslI CCNNNNNNNGG 7 cut(s) 382, 397, 432, 445, 547, 685, 775
BsmAI GTCTC 2 cut(s) 233, 398
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 2 cut(s) 104, 564
BsnI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BsoBI CYCGRG 3 cut(s) 555, 613, 659
Bsp120I GGGCCC 1 cut(s) 765
Bsp1286I GDGCHC 3 cut(s) 117, 390, 769
Bsp143I GATC 6 cut(s) 405, 606, 722, 733, 894, 933
Bsp19I CCATGG 1 cut(s) 268
BspACI CCGC 8 cut(s) 7, 276, 439, 499, 552, 794, 815, 953
BspANI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BspCNI CTCAG 4 cut(s) 77, 106, 468, 964
BspFNI CGCG 3 cut(s) 9, 192, 501
BspLI GGNNCC 8 cut(s) 402, 437, 638, 724, 766, 767, 768, 951
BspPI GGATC 4 cut(s) 601, 717, 730, 741
BsrBI CCGCTC 2 cut(s) 441, 554
BsrDI GCAATG 1 cut(s) 810
BsrFI RCCGGY 3 cut(s) 250, 639, 715
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 3 cut(s) 250, 639, 715
BssECI CCNNGG 6 cut(s) 268, 444, 613, 729, 768, 901
BssMI GATC 6 cut(s) 405, 606, 722, 733, 894, 933
BssT1I CCWWGG 3 cut(s) 268, 444, 729
Bst4CI ACNGT 1 cut(s) 392
BstBAI YACGTR 2 cut(s) 338, 747
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 6 cut(s) 44, 64, 93, 243, 476, 972
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 3 cut(s) 178, 736, 886
BstFNI CGCG 3 cut(s) 9, 192, 501
BstH2I RGCGCY 1 cut(s) 776
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 6 cut(s) 408, 609, 725, 736, 897, 936
BstMAI GTCTC 2 cut(s) 233, 398
BstMBI GATC 6 cut(s) 405, 606, 722, 733, 894, 933
BstMCI CGRYCG 1 cut(s) 644
BstMWI GCNNNNNNNGC 8 cut(s) 282, 332, 341, 347, 528, 557, 655, 664
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 5 cut(s) 396, 612, 613, 768, 901
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 3 cut(s) 9, 192, 501
BstV1I GCAGC 4 cut(s) 353, 404, 667, 777
BstX2I RGATCY 1 cut(s) 722
BstXI CCANNNNNNTGG 1 cut(s) 946
BstYI RGATCY 1 cut(s) 722
Bsu36I CCTNAGG 1 cut(s) 93
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 3 cut(s) 178, 736, 886
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 3 cut(s) 72, 710, 1012
Cac8I GCNNGC 1 cut(s) 591
CfoI GCGC 1 cut(s) 775
Cfr10I RCCGGY 3 cut(s) 250, 639, 715
Cfr13I GGNCC 8 cut(s) 4, 70, 86, 180, 424, 765, 766, 949
Cfr9I CCCGGG 1 cut(s) 613
CseI GACGC 1 cut(s) 181
Csp6I GTAC 4 cut(s) 206, 310, 322, 850
CspAI ACCGGT 1 cut(s) 250
CviQI GTAC 4 cut(s) 206, 310, 322, 850
DdeI CTNAG 6 cut(s) 44, 64, 93, 243, 476, 972
DpnI GATC 6 cut(s) 407, 608, 724, 735, 896, 935
DpnII GATC 6 cut(s) 405, 606, 722, 733, 894, 933
EaeI YGGCCR 2 cut(s) 108, 549
Eco130I CCWWGG 3 cut(s) 268, 444, 729
Eco24I GRGCYC 2 cut(s) 117, 769
Eco47I GGWCC 5 cut(s) 4, 70, 86, 180, 949
Eco72I CACGTG 1 cut(s) 338
Eco81I CCTNAGG 1 cut(s) 93
Eco88I CYCGRG 3 cut(s) 555, 613, 659
EcoO109I RGGNCCY 2 cut(s) 765, 766
EcoRI GAATTC 1 cut(s) 134
EcoT14I CCWWGG 3 cut(s) 268, 444, 729
EcoT38I GRGCYC 2 cut(s) 117, 769
ErhI CCWWGG 3 cut(s) 268, 444, 729
Esp3I CGTCTC 1 cut(s) 398
FaqI GGGAC 2 cut(s) 104, 564
FauI CCCGC 2 cut(s) 506, 960
FbaI TGATCA 1 cut(s) 894
Fnu4HI GCNGC 7 cut(s) 277, 342, 418, 552, 656, 791, 794
FokI GGATG 3 cut(s) 185, 723, 893
FriOI GRGCYC 2 cut(s) 117, 769
Fsp4HI GCNGC 7 cut(s) 277, 342, 418, 552, 656, 791, 794
FspBI CTAG 1 cut(s) 680
GlaI GCGC 1 cut(s) 774
GluI GCNGC 7 cut(s) 277, 342, 418, 552, 656, 791, 794
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 3 cut(s) 242, 546, 671
HaeII RGCGCY 1 cut(s) 776
HaeIII GGCC 6 cut(s) 110, 186, 425, 551, 767, 860
HgaI GACGC 1 cut(s) 181
HhaI GCGC 1 cut(s) 775
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 7 cut(s) 48, 458, 467, 577, 601, 662, 910
Hpy166II GTNNAC 3 cut(s) 70, 691, 835
Hpy188I TCNGA 7 cut(s) 197, 405, 606, 646, 741, 753, 981
Hpy188III TCNNGA 3 cut(s) 525, 700, 1006
Hpy8I GTNNAC 3 cut(s) 70, 691, 835
Hpy99I CGWCG 3 cut(s) 26, 59, 416
HpyAV CCTTC 6 cut(s) 197, 269, 812, 814, 862, 871
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 2 cut(s) 337, 746
HpyCH4V TGCA 5 cut(s) 100, 285, 341, 380, 810
HpyF10VI GCNNNNNNNGC 8 cut(s) 282, 332, 341, 347, 528, 557, 655, 664
HpyF3I CTNAG 6 cut(s) 44, 64, 93, 243, 476, 972
HpySE526I ACGT 2 cut(s) 337, 746
HspAI GCGC 1 cut(s) 773
Ksp22I TGATCA 1 cut(s) 894
Kzo9I GATC 6 cut(s) 405, 606, 722, 733, 894, 933
LmnI GCTCC 9 cut(s) 223, 385, 406, 441, 446, 636, 646, 652, 886
Lsp1109I GCAGC 4 cut(s) 353, 404, 667, 777
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 680
MaeII ACGT 2 cut(s) 337, 746
MaeIII GTNAC 3 cut(s) 479, 669, 708
MalI GATC 6 cut(s) 407, 608, 724, 735, 896, 935
MbiI CCGCTC 2 cut(s) 441, 554
MboI GATC 6 cut(s) 405, 606, 722, 733, 894, 933
MboII GAAGA 3 cut(s) 25, 877, 886
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 3 cut(s) 117, 390, 769
MluCI AATT 1 cut(s) 134
MlyI GAGTC 6 cut(s) 42, 467, 476, 586, 671, 919
MmeI TCCRAC 1 cut(s) 624
MseI TTAA 1 cut(s) 927
MslI CAYNNNNRTG 4 cut(s) 267, 830, 842, 944
MspR9I CCNGG 5 cut(s) 398, 614, 615, 770, 903
MvnI CGCG 3 cut(s) 9, 192, 501
MwoI GCNNNNNNNGC 8 cut(s) 282, 332, 341, 347, 528, 557, 655, 664
NciI CCSGG 5 cut(s) 398, 614, 615, 770, 903
NcoI CCATGG 1 cut(s) 268
NdeII GATC 6 cut(s) 405, 606, 722, 733, 894, 933
NlaIV GGNNCC 8 cut(s) 402, 437, 638, 724, 766, 767, 768, 951
NmeAIII GCCGAG 2 cut(s) 136, 401
NmuCI GTSAC 2 cut(s) 479, 708
NspI RCATGY 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 555
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PfeI GAWTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 547
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 7 cut(s) 278, 343, 419, 553, 657, 792, 795
PleI GAGTC 6 cut(s) 42, 466, 475, 585, 670, 918
PmaCI CACGTG 1 cut(s) 338
PmlI CACGTG 1 cut(s) 338
PpsI GAGTC 6 cut(s) 42, 466, 475, 585, 670, 918
Ppu21I YACGTR 2 cut(s) 338, 747
PscI ACATGT 1 cut(s) 318
PspCI CACGTG 1 cut(s) 338
PspFI CCCAGC 1 cut(s) 560
PspN4I GGNNCC 8 cut(s) 402, 437, 638, 724, 766, 767, 768, 951
PspOMI GGGCCC 1 cut(s) 765
PspPI GGNCC 8 cut(s) 4, 70, 86, 180, 424, 765, 766, 949
PspXI VCTCGAGB 1 cut(s) 555
PsuI RGATCY 1 cut(s) 722
RsaI GTAC 4 cut(s) 207, 311, 323, 851
RsaNI GTAC 4 cut(s) 206, 310, 322, 850
RseI CAYNNNNRTG 4 cut(s) 267, 830, 842, 944
SaqAI TTAA 1 cut(s) 927
SatI GCNGC 7 cut(s) 277, 342, 418, 552, 656, 791, 794
Sau3AI GATC 6 cut(s) 405, 606, 722, 733, 894, 933
Sau96I GGNCC 8 cut(s) 4, 70, 86, 180, 424, 765, 766, 949
ScaI AGTACT 1 cut(s) 851
SchI GAGTC 6 cut(s) 42, 467, 476, 586, 671, 919
ScrFI CCNGG 5 cut(s) 398, 614, 615, 770, 903
SduI GDGCHC 3 cut(s) 117, 390, 769
SfaNI GCATC 1 cut(s) 475
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 5 cut(s) 4, 70, 86, 180, 949
SlaI CTCGAG 1 cut(s) 555
SmaI CCCGGG 1 cut(s) 615
SmiMI CAYNNNNRTG 4 cut(s) 267, 830, 842, 944
SmlI CTYRAG 3 cut(s) 450, 555, 700
SmoI CTYRAG 3 cut(s) 450, 555, 700
Sse9I AATT 1 cut(s) 134
SsiI CCGC 8 cut(s) 7, 276, 439, 499, 552, 794, 815, 953
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 5 cut(s) 396, 612, 613, 768, 901
StyI CCWWGG 3 cut(s) 268, 444, 729
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 2 cut(s) 340, 749
TaqI TCGA 3 cut(s) 24, 556, 625
TasI AATT 1 cut(s) 134
TatI WGTACW 2 cut(s) 309, 849
TauI GCSGC 3 cut(s) 279, 554, 796
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 1 cut(s) 927
Tru9I TTAA 1 cut(s) 927
TscAI CASTG 2 cut(s) 72, 717
TseFI GTSAC 2 cut(s) 479, 708
TseI GCWGC 4 cut(s) 341, 417, 655, 790
Tsp45I GTSAC 2 cut(s) 479, 708
TspDTI ATGAA 8 cut(s) 42, 94, 127, 147, 251, 295, 558, 878
TspGWI ACGGA 3 cut(s) 159, 352, 854
TspMI CCCGGG 1 cut(s) 613
TspRI CASTG 2 cut(s) 72, 717
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 5 cut(s) 4, 70, 86, 180, 949
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XhoI CTCGAG 1 cut(s) 555
XmaI CCCGGG 1 cut(s) 613
XspI CTAG 1 cut(s) 680
ZrmI AGTACT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.