MD00G1099400.v1.1

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002192
Physical Location & Seq
Reverse (-)
20797816 .. 20801181
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1099400.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCTCCACCTCTCCCTCGCTTACTCCTCCTCTCTTCTCTCCTCCTACTCTGCCTCACCATCCCCTCCTTAGCCCACAGTGGCCACCACCACGACGATGAAACCGAACCCGACACCAACGACAGAGAAACCCCGCATGACCTTCGTTCGAGGCCTCTAATACTAGTCAAGATTTGGTGCCTCATCATAATATTTTTTGGGACGTTCGTACCCGGAGTTTCGCCTTATTTCTTCAAGTGGAATGAAGGGTTCTTGGTTTTGGGTATCCAGTTTGCTGGGGGTGTGTTTTTCGGTACGGCTATGATGCACTTTCTGAGTGATTCCGATGAGACTTTCAAGGACTTGACCCAAGAAAAATACCCTTTTGCGTTTATGTTGGCTTGTGGGGGCTTCTTGCTTACAATGCTTGCTGATTGTGTCATTTCCTATGTGTTCCTCAAGAAGAAGAGTGTTGTGTCTGCTGCTGATCTTCAGGTTCGGGGTAATACCAAGCAGGGAAAAGGTGGTCAATATGGCACTCAGCCATACCCAAAACACTGGGTTGCAAGCGCTACTTCACTTGGGGACAACATCTTGTTGATAGTAGCCTTGTGTTTCCACTCTGTGTTTGAGGGCATTGCAATTGGAGTTGCTGAGACTAAAGCCGATGCTTGGAAAGCCTTATGGACAATTTCTCTTCACAAGGTATTTGCCGCCATTGCCATGGGCATTGCTCTCCTTCGAATGATGCCTAACCGTCCCTTCTTGTCATGTGCTGCCTGCGCTTTTGCATTTGCTATTTCAAGTCCTATTGGTGTGGCAATTGGAATCCTAATAGATGCGACAACCCAAGGAGCTGTGGCTGATTGGATATTCGCGATTTCAATGGGTCTAGCATGTGGAGTGTTCATCTACGTAGCAATAAACCATTTATTGGCGAAGGGCTATAGACCCGACAGAGCAGTTTCAGTCGACAAACCCCAGTACAAGTTTCTGGCAGTTTTGTTGGGTATTGGGGTAATAGCTGTTGTGATGATTTGGGACACTTCAAGTCATTGTTCGTGCACGATTTGTTTGTGGAAGATTGCGAGAAGTTTAGATTTGTAA

Protein Analysis

362

Amino Acids

39.4

Weight (kDa)

7.58

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 55 - 337 7.2e-48 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 177
AccB7I CCANNNNNTGG 1 cut(s) 913
AccI GTMKAC 1 cut(s) 951
AccII CGCG 1 cut(s) 857
AciI CCGC 2 cut(s) 134, 693
AcoI YGGCCR 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 455
AdeI CACNNNGTG 1 cut(s) 604
AfaI GTAC 3 cut(s) 210, 295, 965
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 651, 913
AgsI TTSAA 5 cut(s) 235, 337, 783, 864, 1029
AhlI ACTAGT 1 cut(s) 163
AluBI AGCT 2 cut(s) 836, 1004
AluI AGCT 2 cut(s) 836, 1004
Alw21I GWGCWC 1 cut(s) 1046
Alw26I GTCTC 2 cut(s) 323, 629
Alw44I GTGCAC 1 cut(s) 1042
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 2 cut(s) 82, 152
ApaLI GTGCAC 1 cut(s) 1042
ApeKI GCWGC 2 cut(s) 461, 755
AspLEI GCGC 2 cut(s) 551, 764
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 721
BaeGI GKGCMC 1 cut(s) 1046
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BalI TGGCCA 1 cut(s) 84
BanI GGYRCC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 1046
BbvI GCAGC 2 cut(s) 448, 742
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 312
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciVI GTATCC 1 cut(s) 275
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 323, 629
BcuI ACTAGT 1 cut(s) 163
BfaI CTAG 2 cut(s) 164, 872
BfmI CTRYAG 1 cut(s) 925
BfoI RGCGCY 1 cut(s) 552
BfuI GTATCC 1 cut(s) 275
BisI GCNGC 3 cut(s) 462, 693, 756
BlsI GCNGC 3 cut(s) 463, 694, 757
Bme1390I CCNGG 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 6, 179
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 2 cut(s) 547, 955
BmsI GCATC 4 cut(s) 294, 637, 717, 808
BmuI ACTGGG 2 cut(s) 547, 955
Bpu10I CCTNAGC 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 895
BsaJI CCNNGG 2 cut(s) 702, 829
Bsc4I CCNNNNNNNGG 2 cut(s) 651, 913
Bse1I ACTGG 3 cut(s) 268, 542, 961
Bse3DI GCAATG 3 cut(s) 615, 696, 708
BseDI CCNNGG 2 cut(s) 702, 829
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 651, 913
BseMI GCAATG 3 cut(s) 615, 696, 708
BseMII CTCAG 3 cut(s) 305, 533, 624
BseNI ACTGG 3 cut(s) 268, 542, 961
BseRI GAGGAG 3 cut(s) 17, 20, 32
BseSI GKGCMC 1 cut(s) 1046
BseXI GCAGC 2 cut(s) 448, 742
BseYI CCCAGC 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 857
BshFI GGCC 2 cut(s) 84, 154
BshNI GGYRCC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 1046
BsiSI CCGG 1 cut(s) 213
BslFI GGGAC 4 cut(s) 214, 578, 723, 1034
BslI CCNNNNNNNGG 2 cut(s) 651, 913
BsmAI GTCTC 2 cut(s) 323, 629
BsmFI GGGAC 4 cut(s) 214, 578, 723, 1034
BsnI GGCC 2 cut(s) 84, 154
Bsp119I TTCGAA 1 cut(s) 721
Bsp1286I GDGCHC 1 cut(s) 1046
Bsp143I GATC 1 cut(s) 466
Bsp19I CCATGG 1 cut(s) 702
Bsp68I TCGCGA 1 cut(s) 857
BspACI CCGC 2 cut(s) 134, 693
BspANI GGCC 2 cut(s) 84, 154
BspCNI CTCAG 3 cut(s) 306, 532, 625
BspFNI CGCG 1 cut(s) 857
BspLI GGNNCC 2 cut(s) 6, 179
BspT104I TTCGAA 1 cut(s) 721
BspT107I GGYRCC 1 cut(s) 177
BsrDI GCAATG 3 cut(s) 615, 696, 708
BsrI ACTGG 3 cut(s) 268, 542, 961
BssECI CCNNGG 2 cut(s) 702, 829
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 702, 829
Bst4CI ACNGT 2 cut(s) 80, 737
Bst6I CTCTTC 3 cut(s) 40, 440, 681
BstBAI YACGTR 1 cut(s) 895
BstBI TTCGAA 1 cut(s) 721
BstC8I GCNNGC 3 cut(s) 408, 547, 760
BstDEI CTNAG 4 cut(s) 70, 314, 519, 633
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 857
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 2 cut(s) 551, 764
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 2 cut(s) 323, 629
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 403, 656, 698, 761
BstNSI RCATGY 1 cut(s) 879
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 925
BstSLI GKGCMC 1 cut(s) 1046
BstSNI TACGTA 1 cut(s) 895
BstUI CGCG 1 cut(s) 857
BstV1I GCAGC 2 cut(s) 448, 742
BstXI CCANNNNNNTGG 3 cut(s) 275, 537, 703
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 2 cut(s) 84, 154
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 85, 535
BtuMI TCGCGA 1 cut(s) 857
Cac8I GCNNGC 3 cut(s) 408, 547, 760
CfoI GCGC 2 cut(s) 551, 764
Csp6I GTAC 3 cut(s) 209, 294, 964
CviAII CATG 4 cut(s) 137, 703, 750, 876
CviQI GTAC 3 cut(s) 209, 294, 964
DdeI CTNAG 4 cut(s) 70, 314, 519, 633
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DraIII CACNNNGTG 1 cut(s) 604
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 3 cut(s) 40, 440, 681
EarI CTCTTC 3 cut(s) 40, 440, 681
Eco105I TACGTA 1 cut(s) 895
Eco130I CCWWGG 2 cut(s) 702, 829
Eco147I AGGCCT 1 cut(s) 154
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 702, 829
ErhI CCWWGG 2 cut(s) 702, 829
FaeI CATG 4 cut(s) 140, 706, 753, 879
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 4 cut(s) 214, 578, 723, 1034
FatI CATG 4 cut(s) 136, 702, 749, 875
FauI CCCGC 1 cut(s) 141
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 3 cut(s) 462, 693, 756
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 3 cut(s) 462, 693, 756
FspBI CTAG 2 cut(s) 164, 872
GlaI GCGC 2 cut(s) 550, 763
GluI GCNGC 3 cut(s) 462, 693, 756
GsaI CCCAGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 2 cut(s) 84, 154
HapII CCGG 1 cut(s) 213
HhaI GCGC 2 cut(s) 551, 764
Hin1II CATG 4 cut(s) 140, 706, 753, 879
Hin6I GCGC 2 cut(s) 549, 762
HinP1I GCGC 2 cut(s) 549, 762
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 2 cut(s) 320, 807
HpaII CCGG 1 cut(s) 213
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 952, 1044
Hpy188I TCNGA 2 cut(s) 315, 325
Hpy188III TCNNGA 3 cut(s) 169, 439, 856
Hpy8I GTNNAC 2 cut(s) 952, 1044
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 5 cut(s) 152, 239, 728, 751, 913
HpyCH4III ACNGT 2 cut(s) 80, 737
HpyCH4IV ACGT 2 cut(s) 203, 894
HpyCH4V TGCA 5 cut(s) 307, 545, 620, 770, 1044
HpyF10VI GCNNNNNNNGC 4 cut(s) 403, 656, 698, 761
HpyF3I CTNAG 4 cut(s) 70, 314, 519, 633
HpySE526I ACGT 2 cut(s) 203, 894
Hsp92II CATG 4 cut(s) 140, 706, 753, 879
HspAI GCGC 2 cut(s) 549, 762
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 10, 833
LpnPI CCDG 9 cut(s) 226, 261, 281, 458, 479, 523, 772, 959, 974
Lsp1109I GCAGC 2 cut(s) 448, 742
LweI GCATC 4 cut(s) 294, 637, 717, 808
MaeI CTAG 2 cut(s) 164, 872
MaeII ACGT 2 cut(s) 203, 894
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 7 cut(s) 27, 223, 454, 457, 461, 668, 1072
MfeI CAATTG 2 cut(s) 621, 801
MhlI GDGCHC 1 cut(s) 1046
MlsI TGGCCA 1 cut(s) 84
MluCI AATT 3 cut(s) 621, 669, 801
MluNI TGGCCA 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 84
MscI TGGCCA 1 cut(s) 84
MslI CAYNNNNRTG 2 cut(s) 96, 701
Msp20I TGGCCA 1 cut(s) 84
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 2 cut(s) 621, 801
MvnI CGCG 1 cut(s) 857
MwoI GCNNNNNNNGC 4 cut(s) 403, 656, 698, 761
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 1 cut(s) 702
NdeII GATC 1 cut(s) 466
NlaIII CATG 4 cut(s) 140, 706, 753, 879
NlaIV GGNNCC 2 cut(s) 6, 179
NruI TCGCGA 1 cut(s) 857
NspI RCATGY 1 cut(s) 879
NspV TTCGAA 1 cut(s) 721
PceI AGGCCT 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 2 cut(s) 320, 807
PflMI CCANNNNNTGG 1 cut(s) 913
PkrI GCNGC 3 cut(s) 463, 694, 757
Ppu21I YACGTR 1 cut(s) 895
PspFI CCCAGC 1 cut(s) 275
PspN4I GGNNCC 2 cut(s) 6, 179
RruI TCGCGA 1 cut(s) 857
RsaI GTAC 3 cut(s) 210, 295, 965
RsaNI GTAC 3 cut(s) 209, 294, 964
RseI CAYNNNNRTG 2 cut(s) 96, 701
SalI GTCGAC 1 cut(s) 950
SatI GCNGC 3 cut(s) 462, 693, 756
Sau3AI GATC 1 cut(s) 466
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 1046
SetI ASST 9 cut(s) 13, 144, 206, 477, 505, 687, 838, 897, 1006
SfaNI GCATC 4 cut(s) 294, 637, 717, 808
SfcI CTRYAG 1 cut(s) 925
SfuI TTCGAA 1 cut(s) 721
SmiMI CAYNNNNRTG 2 cut(s) 96, 701
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SnaBI TACGTA 1 cut(s) 895
SpeI ACTAGT 1 cut(s) 163
Sse9I AATT 3 cut(s) 621, 669, 801
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 2 cut(s) 134, 693
SspI AATATT 1 cut(s) 192
SspMI CTAG 2 cut(s) 164, 872
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 2 cut(s) 702, 829
TaaI ACNGT 2 cut(s) 80, 737
TaiI ACGT 2 cut(s) 206, 897
TaqI TCGA 3 cut(s) 149, 721, 951
TasI AATT 3 cut(s) 621, 669, 801
TatI WGTACW 1 cut(s) 963
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 2 cut(s) 320, 807
TscAI CASTG 2 cut(s) 85, 542
TseI GCWGC 2 cut(s) 461, 755
TspDTI ATGAA 3 cut(s) 114, 258, 877
TspRI CASTG 2 cut(s) 85, 542
Van91I CCANNNNNTGG 1 cut(s) 913
VneI GTGCAC 1 cut(s) 1042
XceI RCATGY 1 cut(s) 879
XmiI GTMKAC 1 cut(s) 951
XspI CTAG 2 cut(s) 164, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.