Rorug05G0284600

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002192
Physical Location & Seq
Reverse (-)
32637220 .. 32638750
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0284600.1

Sequence Viewer

Length: 660 bp
ATGTTTCTGACCTTGTTTAAACTTAATTGGACTGACAAAATGCATAGTATCTCAGTGAGTGGTCTTGCCTTGATACCTGGAGTTGTTGGGCGTTTTGACTCAACAAATGGTGTCAGGGTTCCACATATCAGGTGGAATGCTTTGCAGATTAGAAAAGACTCTTTAATTTTGGATAATGTTGGAACCCATCACGTCTATTTCGTTCACTCTTACAAAGCGATGCCATCGGATGAAAACAAAGAATGGGTTTCATCTACCTGCAACTATGGTGACAATTTTATTGCGTCTGTTAGAAGGGGAAATGTGCATGCGGTTCAATTCCACCCCGAAAAAAGTGGAGGCAAGATATCATTTTCTCACTCTTATTACTCTATCCAGACTCACCCTTGGAGAAGACAGAAGCCTATGGAAGGGAAAGGTTCCAAACTTGCGAAGAGGGTTGTTGCTTGGCTGGATGTGAGGACGAATGACAAAGGAGATCTTGTTGTAATCAAAGGAGACCAATATGATGTAAGAGAGCATACAAAAGAGAGTGAGATATTCCAAGAACTCTCGACTGCATTCTTGTATCATTTGTGTGGTTCTTTGGACCTGACTATTGGGTGCAAACTGTCACAATTAAAGAAATATCAGAAACCCTCAGCCTTCGGTGCATCATAA

Protein Analysis

219

Amino Acids

24.86

Weight (kDa)

9.45

Isoelectric Point (pI)

29.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 41 - 114 6.5e-08 Glutamine amidotransferase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 266
AciI CCGC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 1 cut(s) 317
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 589
AsuHPI GGTGA 2 cut(s) 281, 374
AvaII GGWCC 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 640
BccI CCATC 2 cut(s) 195, 232
BciT130I CCWGG 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 266
BglII AGATCT 1 cut(s) 478
Bme1390I CCNGG 1 cut(s) 78
Bme18I GGWCC 1 cut(s) 589
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 589
BmiI GGNNCC 3 cut(s) 120, 184, 421
BmrFI CCNGG 1 cut(s) 78
BmsI GCATC 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 640
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 386
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 410
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 2 cut(s) 235, 460
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 2 cut(s) 66, 654
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 1 cut(s) 492
BsmI GAATGC 2 cut(s) 142, 560
Bso31I GGTCTC 1 cut(s) 492
Bsp143I GATC 1 cut(s) 478
BspACI CCGC 1 cut(s) 311
BspCNI CTCAG 2 cut(s) 65, 653
BspLI GGNNCC 3 cut(s) 120, 184, 421
BspMI ACCTGC 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 492
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 1 cut(s) 478
BssT1I CCWWGG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 612
Bst6I CTCTTC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 52, 640
BstENI CCTNNNNNAGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 235, 460
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 76
BstV2I GAAGAC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BtgZI GCGATG 1 cut(s) 233
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 2 cut(s) 235, 460
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 589
CseI GACGC 1 cut(s) 273
CviAII CATG 1 cut(s) 308
CviJI RGCY 3 cut(s) 403, 451, 644
CviKI_1 RGCY 3 cut(s) 403, 451, 644
DdeI CTNAG 2 cut(s) 52, 640
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
DraI TTTAAA 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 386
Eco31I GGTCTC 1 cut(s) 492
Eco32I GATATC 1 cut(s) 348
Eco47I GGWCC 1 cut(s) 589
EcoNI CCTNNNNNAGG 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 76
EcoRV GATATC 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 1 cut(s) 311
FaiI YATR 8 cut(s) 45, 126, 267, 309, 407, 507, 522, 658
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FatI CATG 1 cut(s) 307
FokI GGATG 2 cut(s) 242, 467
GsuI CTGGAG 1 cut(s) 99
HgaI GACGC 1 cut(s) 273
Hin1II CATG 1 cut(s) 311
HinfI GANTC 3 cut(s) 98, 158, 379
HphI GGTGA 2 cut(s) 281, 374
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 9, 229, 633
Hpy188III TCNNGA 2 cut(s) 376, 553
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 3 cut(s) 288, 404, 655
HpyCH4III ACNGT 1 cut(s) 612
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 7 cut(s) 43, 145, 261, 307, 560, 606, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 2 cut(s) 52, 640
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 1 cut(s) 311
Kzo9I GATC 1 cut(s) 478
LpnPI CCDG 8 cut(s) 63, 90, 100, 115, 271, 389, 437, 605
LweI GCATC 1 cut(s) 210
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 269, 612
MalI GATC 1 cut(s) 480
MboI GATC 1 cut(s) 478
MboII GAAGA 2 cut(s) 405, 445
MflI RGATCY 1 cut(s) 478
MluCI AATT 5 cut(s) 25, 165, 274, 317, 617
MlyI GAGTC 3 cut(s) 92, 152, 373
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 4 cut(s) 332, 429, 453, 649
Mph1103I ATGCAT 1 cut(s) 45
MseI TTAA 4 cut(s) 18, 24, 164, 620
MslI CAYNNNNRTG 1 cut(s) 576
MspR9I CCNGG 1 cut(s) 78
MssI GTTTAAAC 1 cut(s) 19
Mva1269I GAATGC 2 cut(s) 142, 560
MvaI CCWGG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 650
NdeII GATC 1 cut(s) 478
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 3 cut(s) 120, 184, 421
NmuCI GTSAC 2 cut(s) 269, 612
NsiI ATGCAT 1 cut(s) 45
NspI RCATGY 1 cut(s) 311
PaeI GCATGC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 2 cut(s) 142, 560
PleI GAGTC 3 cut(s) 92, 152, 373
PmeI GTTTAAAC 1 cut(s) 19
PpsI GAGTC 3 cut(s) 92, 152, 373
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 3 cut(s) 120, 184, 421
PspPI GGNCC 1 cut(s) 589
PsuI RGATCY 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 576
SaqAI TTAA 4 cut(s) 18, 24, 164, 620
Sau3AI GATC 1 cut(s) 478
Sau96I GGNCC 1 cut(s) 589
SchI GAGTC 3 cut(s) 92, 152, 373
ScrFI CCNGG 1 cut(s) 78
SetI ASST 7 cut(s) 14, 79, 134, 195, 260, 421, 594
SfaNI GCATC 1 cut(s) 210
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 1 cut(s) 576
SphI GCATGC 1 cut(s) 311
Sse9I AATT 5 cut(s) 25, 165, 274, 317, 617
SsiI CCGC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 612
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 554
TasI AATT 5 cut(s) 25, 165, 274, 317, 617
Tru1I TTAA 4 cut(s) 18, 24, 164, 620
Tru9I TTAA 4 cut(s) 18, 24, 164, 620
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 2 cut(s) 269, 612
Tsp45I GTSAC 2 cut(s) 269, 612
TspDTI ATGAA 2 cut(s) 240, 246
TspRI CASTG 1 cut(s) 60
VpaK11BI GGWCC 1 cut(s) 589
XagI CCTNNNNNAGG 1 cut(s) 408
XceI RCATGY 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.