pycom03g13820

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002192
Physical Location & Seq
Forward (+)
14907315 .. 14908980
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13820.1

Sequence Viewer

Length: 1038 bp
ATGGCTCCACCTCTCCCTCGCTTACTCCTCTTCTGTTCTCTCCTCCTCCTTTGCCTCACCATCCCCGCTTTAGCCCACAGCGGCCACGACGACGACGACGAAACTGAACCCGCCACCAACGACAGCGAAACCCGGCATGACCTCCGTTCGAGGCCTCTAATACTAGTCAAGATTTGGTGCCTCATCATAATATTTTTTGGGACGTTCGTACCCGGAGTTTCGCCTTATTTCTTCAAGTGGAATGAAGGGTTCTTGGTTTTGGGTACGCAGTTTGCTGGAGGTGTGTTTTTTGGTACGGCGATGATGCACTTTCTAAGTGATTCCAATGAGACTTTCAAGGACTTGACCAAAAAAGAATACCCTTTTGCATTTATGTTGGCTTGTGGGGGATTCTTGCTTACAATGCTTGCTGATTGTGTCATTTCCTATGTGTTCCTCAAGAATAAGAGCGGTGTGTCTGCTGCTGATCTTCAGGTTCAGGGTAATATTGAGCAGGGAAAGGGTGGTCAATATGGCACTCAGAGCCATAACCACTTTGCAAATGCAGTGGTTGCAAGCGCTACTTCACTTGGGGACAGCATCTTGTTGATAGTAGCCTTGTGTTTCCACTCTGTGTTTGAGGGCATTGCAATTGGAGTCGCTGAGACTAAAGCCGATGCTTGGAAAGCCTTATGGACAATTTCTCTTCACAAGGTATTTGCGGCCATTGCCATGGGCATTGCTCTCCTTCGAATGATGCCTAACCGTCCCTTCTTATCATGCGCTGCCTACGCTTTTGCATTTGCTATTTCAAGTCCTATTGGTGTGGCAATCGGAATCCTAATAGATGCGACAACCCAAGGAGCTGTGGCTGATTGGATATTCGCGATTTCAATGGCTCTAGCATGTGGAGTGTTCATATACGTAGCAATAAACCATTTATTGGCAAAGGGCTATACACCCGACAGAGCAGTTTCAGTTGACAAGCCCCAGTACAAGTTTCTGGCAGTTTTGTTAGGCATTGGGGTAATAGCTGTTGTGATGATTTGGGACACTTGA

Protein Analysis

346

Amino Acids

37.29

Weight (kDa)

5.96

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 55 - 340 5.6e-51 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 177
AccB7I CCANNNNNTGG 1 cut(s) 922
AccBSI CCGCTC 1 cut(s) 450
AccII CGCG 1 cut(s) 866
AciI CCGC 5 cut(s) 66, 81, 111, 450, 701
AcoI YGGCCR 2 cut(s) 82, 702
AcuI CTGAAG 1 cut(s) 455
AdeI CACNNNGTG 1 cut(s) 613
AfaI GTAC 4 cut(s) 210, 265, 295, 974
AfeI AGCGCT 1 cut(s) 559
AfiI CCNNNNNNNGG 2 cut(s) 660, 922
AgsI TTSAA 4 cut(s) 235, 337, 792, 873
AhlI ACTAGT 1 cut(s) 163
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 2 cut(s) 845, 1013
AluI AGCT 2 cut(s) 845, 1013
Alw26I GTCTC 2 cut(s) 323, 638
Aor51HI AGCGCT 1 cut(s) 559
AoxI GGCC 3 cut(s) 82, 152, 702
ApeKI GCWGC 2 cut(s) 461, 764
AspLEI GCGC 2 cut(s) 560, 764
AsuC2I CCSGG 2 cut(s) 133, 213
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 730
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BanI GGYRCC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 448, 751
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 312
BcnI CCSGG 2 cut(s) 133, 213
BcoDI GTCTC 2 cut(s) 323, 638
BcuI ACTAGT 1 cut(s) 163
BfaI CTAG 2 cut(s) 164, 881
BfoI RGCGCY 1 cut(s) 561
BisI GCNGC 4 cut(s) 82, 462, 702, 765
BlsI GCNGC 4 cut(s) 83, 463, 703, 766
Bme1390I CCNGG 2 cut(s) 133, 213
BmiI GGNNCC 2 cut(s) 6, 179
BmrFI CCNGG 2 cut(s) 133, 213
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 5 cut(s) 294, 588, 646, 726, 817
BmuI ACTGGG 1 cut(s) 964
BpmI CTGGAG 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 730
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 133, 213
BsaAI YACGTR 1 cut(s) 904
BsaJI CCNNGG 2 cut(s) 711, 838
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 922
Bse1I ACTGG 1 cut(s) 970
Bse3DI GCAATG 3 cut(s) 624, 705, 717
BseDI CCNNGG 2 cut(s) 711, 838
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 2 cut(s) 660, 922
BseMI GCAATG 3 cut(s) 624, 705, 717
BseMII CTCAG 2 cut(s) 533, 633
BseNI ACTGG 1 cut(s) 970
BseRI GAGGAG 3 cut(s) 17, 32, 35
BseXI GCAGC 2 cut(s) 448, 751
Bsh1236I CGCG 1 cut(s) 866
BshFI GGCC 3 cut(s) 84, 154, 704
BshNI GGYRCC 1 cut(s) 177
BsiSI CCGG 2 cut(s) 133, 213
BslFI GGGAC 3 cut(s) 214, 587, 732
BslI CCNNNNNNNGG 2 cut(s) 660, 922
BsmAI GTCTC 2 cut(s) 323, 638
BsmFI GGGAC 3 cut(s) 214, 587, 732
BsnI GGCC 3 cut(s) 84, 154, 704
Bsp119I TTCGAA 1 cut(s) 730
Bsp143I GATC 1 cut(s) 466
Bsp19I CCATGG 1 cut(s) 711
Bsp68I TCGCGA 1 cut(s) 866
BspACI CCGC 5 cut(s) 66, 81, 111, 450, 701
BspANI GGCC 3 cut(s) 84, 154, 704
BspCNI CTCAG 2 cut(s) 532, 634
BspFNI CGCG 1 cut(s) 866
BspLI GGNNCC 2 cut(s) 6, 179
BspT104I TTCGAA 1 cut(s) 730
BspT107I GGYRCC 1 cut(s) 177
BsrBI CCGCTC 1 cut(s) 450
BsrDI GCAATG 3 cut(s) 624, 705, 717
BsrI ACTGG 1 cut(s) 970
BssECI CCNNGG 2 cut(s) 711, 838
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 711, 838
Bst4CI ACNGT 1 cut(s) 746
Bst6I CTCTTC 2 cut(s) 35, 690
BstAPI GCANNNNNTGC 1 cut(s) 551
BstBAI YACGTR 1 cut(s) 904
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 2 cut(s) 408, 556
BstDEI CTNAG 3 cut(s) 314, 519, 642
BstDSI CCRYGG 1 cut(s) 711
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 866
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 2 cut(s) 560, 764
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 2 cut(s) 323, 638
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 6 cut(s) 403, 522, 551, 665, 707, 770
BstNSI RCATGY 1 cut(s) 888
BstSCI CCNGG 2 cut(s) 131, 211
BstSNI TACGTA 1 cut(s) 904
BstUI CGCG 1 cut(s) 866
BstV1I GCAGC 2 cut(s) 448, 751
BstXI CCANNNNNNTGG 1 cut(s) 712
BsuRI GGCC 3 cut(s) 84, 154, 704
BtgI CCRYGG 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 314
BtsCI GGATG 1 cut(s) 60
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 1 cut(s) 552
BtuMI TCGCGA 1 cut(s) 866
Cac8I GCNNGC 2 cut(s) 408, 556
CfoI GCGC 2 cut(s) 560, 764
Csp6I GTAC 4 cut(s) 209, 264, 294, 973
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviAII CATG 4 cut(s) 137, 712, 759, 885
CviQI GTAC 4 cut(s) 209, 264, 294, 973
DdeI CTNAG 3 cut(s) 314, 519, 642
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DraIII CACNNNGTG 1 cut(s) 613
EaeI YGGCCR 2 cut(s) 82, 702
Eam1104I CTCTTC 2 cut(s) 35, 690
EarI CTCTTC 2 cut(s) 35, 690
Eco105I TACGTA 1 cut(s) 904
Eco130I CCWWGG 2 cut(s) 711, 838
Eco147I AGGCCT 1 cut(s) 154
Eco47III AGCGCT 1 cut(s) 559
Eco57I CTGAAG 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 711, 838
ErhI CCWWGG 2 cut(s) 711, 838
FaeI CATG 4 cut(s) 140, 715, 762, 888
FalI AAGNNNNNCTT 2 cut(s) 547, 579
FaqI GGGAC 3 cut(s) 214, 587, 732
FatI CATG 4 cut(s) 136, 711, 758, 884
FauI CCCGC 2 cut(s) 73, 118
Fnu4HI GCNGC 4 cut(s) 82, 462, 702, 765
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 4 cut(s) 82, 462, 702, 765
FspBI CTAG 2 cut(s) 164, 881
GlaI GCGC 2 cut(s) 559, 763
GluI GCNGC 4 cut(s) 82, 462, 702, 765
GsuI CTGGAG 1 cut(s) 297
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 3 cut(s) 84, 154, 704
HapII CCGG 2 cut(s) 133, 213
HhaI GCGC 2 cut(s) 560, 764
Hin1II CATG 4 cut(s) 140, 715, 762, 888
Hin6I GCGC 2 cut(s) 558, 762
HinP1I GCGC 2 cut(s) 558, 762
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 4 cut(s) 320, 390, 636, 816
HpaII CCGG 2 cut(s) 133, 213
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 961
Hpy188I TCNGA 2 cut(s) 522, 815
Hpy188III TCNNGA 3 cut(s) 169, 439, 865
Hpy8I GTNNAC 1 cut(s) 961
Hpy99I CGWCG 4 cut(s) 92, 95, 98, 101
HpyAV CCTTC 3 cut(s) 239, 737, 760
HpyCH4III ACNGT 1 cut(s) 746
HpyCH4IV ACGT 2 cut(s) 203, 903
HpyCH4V TGCA 7 cut(s) 307, 368, 539, 545, 554, 629, 779
HpyF10VI GCNNNNNNNGC 6 cut(s) 403, 522, 551, 665, 707, 770
HpyF3I CTNAG 3 cut(s) 314, 519, 642
HpySE526I ACGT 2 cut(s) 203, 903
Hsp92II CATG 4 cut(s) 140, 715, 762, 888
HspAI GCGC 2 cut(s) 558, 762
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 10, 842
LpnPI CCDG 8 cut(s) 146, 226, 261, 458, 464, 479, 968, 983
Lsp1109I GCAGC 2 cut(s) 448, 751
LweI GCATC 5 cut(s) 294, 588, 646, 726, 817
MaeI CTAG 2 cut(s) 164, 881
MaeII ACGT 2 cut(s) 203, 903
MalI GATC 1 cut(s) 468
MbiI CCGCTC 1 cut(s) 450
MboI GATC 1 cut(s) 466
MboII GAAGA 4 cut(s) 22, 223, 461, 677
MfeI CAATTG 1 cut(s) 630
MluCI AATT 2 cut(s) 630, 678
MlyI GAGTC 1 cut(s) 645
MslI CAYNNNNRTG 1 cut(s) 710
MspA1I CMGCKG 1 cut(s) 81
MspI CCGG 2 cut(s) 133, 213
MspR9I CCNGG 2 cut(s) 133, 213
MunI CAATTG 1 cut(s) 630
MvnI CGCG 1 cut(s) 866
MwoI GCNNNNNNNGC 6 cut(s) 403, 522, 551, 665, 707, 770
NciI CCSGG 2 cut(s) 133, 213
NcoI CCATGG 1 cut(s) 711
NdeII GATC 1 cut(s) 466
NlaIII CATG 4 cut(s) 140, 715, 762, 888
NlaIV GGNNCC 2 cut(s) 6, 179
NruI TCGCGA 1 cut(s) 866
NspI RCATGY 1 cut(s) 888
NspV TTCGAA 1 cut(s) 730
PceI AGGCCT 1 cut(s) 154
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 96
PfeI GAWTC 3 cut(s) 320, 390, 816
PflMI CCANNNNNTGG 1 cut(s) 922
PkrI GCNGC 4 cut(s) 83, 463, 703, 766
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
Ppu21I YACGTR 1 cut(s) 904
PspN4I GGNNCC 2 cut(s) 6, 179
RruI TCGCGA 1 cut(s) 866
RsaI GTAC 4 cut(s) 210, 265, 295, 974
RsaNI GTAC 4 cut(s) 209, 264, 294, 973
RseI CAYNNNNRTG 1 cut(s) 710
SatI GCNGC 4 cut(s) 82, 462, 702, 765
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 1 cut(s) 645
ScrFI CCNGG 2 cut(s) 133, 213
SetI ASST 9 cut(s) 13, 144, 206, 283, 477, 696, 847, 906, 1015
SfaNI GCATC 5 cut(s) 294, 588, 646, 726, 817
SfuI TTCGAA 1 cut(s) 730
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SnaBI TACGTA 1 cut(s) 904
SpeI ACTAGT 1 cut(s) 163
Sse9I AATT 2 cut(s) 630, 678
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 5 cut(s) 66, 81, 111, 450, 701
SspI AATATT 2 cut(s) 192, 487
SspMI CTAG 2 cut(s) 164, 881
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 131, 211
StyI CCWWGG 2 cut(s) 711, 838
TaaI ACNGT 1 cut(s) 746
TaiI ACGT 2 cut(s) 206, 906
TaqI TCGA 2 cut(s) 149, 730
TasI AATT 2 cut(s) 630, 678
TatI WGTACW 1 cut(s) 972
TauI GCSGC 2 cut(s) 84, 704
TfiI GAWTC 3 cut(s) 320, 390, 816
TscAI CASTG 1 cut(s) 552
TseI GCWGC 2 cut(s) 461, 764
TspDTI ATGAA 2 cut(s) 258, 886
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 1 cut(s) 552
Van91I CCANNNNNTGG 1 cut(s) 922
XceI RCATGY 1 cut(s) 888
XspI CTAG 2 cut(s) 164, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.