MD01G1199600.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001452
Physical Location & Seq
Reverse (-)
29675369 .. 29678045
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1199600.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGACTGACACACCCTCCTCAAGAACCAGAACTCTAATAAATTGTGACGGAGTAAGGCTGTTTGGAGGTGGTTGGGAAGCTGGTGAAATGGAAGACGGTGGCCTCCCTGTGAGTAAATGGAAGGACCTGAACACTGACATACTACTAATGAGGATTTTACAGTCTTTCGACATCTTTGAGTTAACGTCGGGCATTGCTCACGTTTGTAGTGCGTGGCGCTTGGTTTGCCGTGATCCTCTGCTGTGGAAAAAGCTTGATTTGTCGATGATGAAATCGGATTTCATCAAATCACAAGTGGAGCCTTATGTCTATGTGACAGACTCGTCCGATAAGACACTGACTCGTATTTTGAAGACTTCATTGAGTCTCAGCCGGGGAAACATAATGACATTGGTTTTCCATTACAACTTATTTGTGAGCGATGATCAGTTGACGTATACTGCTGAAAGGTGCCCACAGCTGAAGCGACTGGTTATGCCGGCTTGGAACAGAATAAAAAAGACTGGAATCTGCAAGGCCATTCGCTGCTGGAAAGAGCTTGAATCGCTAACAATGCCTAGCATAGCGAATCCCCCGTACCTCTTGGAAGAGATTAAAAGGAACTGCAAAAATTTCAGTGAACTGAAGATCATGGGTCCTTGTGATATGTTCTTTGCTTCAACACTAGTTGAACATCTTCCACAACTGAAGGTGCTAAGCCTCCGGTGCACGACGCTCTTAAAGGATGTTTTGATCTTCATCTTGGATCACCTAGAACACCTGGAAGTTCTCAACATCTCACATTGCCGTCTAATCGAAATTCCTCCGCCTCCTGCAACTAGGAGACTTGTCAGAAATCTCGATCCGCTTATTCTCGAGAAGGCCTCTCGGTTGCAAAGATTTTTAACATGCATGCAGGACTCTTGCGTGATGTGCCAAAGGACCAAAAACGACGAGGGAATCATGAGGTGGTATAATTACGAAGGGCTATGGAAAGAAGATGAGGTGAGCTCCCTAGCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

335

Amino Acids

38.52

Weight (kDa)

8.15

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 48 - 87 6.6e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 450
AccI GTMKAC 1 cut(s) 437
AciI CCGC 2 cut(s) 806, 845
AclWI GGATC 3 cut(s) 227, 753, 836
AcsI RAATTY 2 cut(s) 610, 798
AcuI CTGAAG 3 cut(s) 482, 644, 707
AfaI GTAC 1 cut(s) 578
AgsI TTSAA 4 cut(s) 352, 542, 660, 671
AhlI ACTAGT 1 cut(s) 664
AjnI CCWGG 1 cut(s) 759
AluBI AGCT 6 cut(s) 80, 253, 460, 538, 990, 998
AluI AGCT 6 cut(s) 80, 253, 460, 538, 990, 998
Alw21I GWGCWC 2 cut(s) 710, 992
Alw26I GTCTC 2 cut(s) 371, 817
Alw44I GTGCAC 1 cut(s) 706
AlwI GGATC 3 cut(s) 227, 753, 836
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 3 cut(s) 100, 516, 861
ApaLI GTGCAC 1 cut(s) 706
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 2 cut(s) 610, 798
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
Asp700I GAANNNNTTC 1 cut(s) 675
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 124, 635, 921
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 3 cut(s) 95, 740, 997
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 3 cut(s) 124, 635, 921
BaeGI GKGCMC 2 cut(s) 455, 710
BanI GGYRCC 1 cut(s) 450
BanII GRGCYC 1 cut(s) 992
BbsI GAAGAC 2 cut(s) 99, 359
Bbv12I GWGCWC 2 cut(s) 710, 992
BbvI GCAGC 1 cut(s) 512
BceAI ACGGC 2 cut(s) 213, 771
BciT130I CCWGG 1 cut(s) 761
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 371, 817
BcuI ACTAGT 1 cut(s) 664
BfaI CTAG 5 cut(s) 558, 665, 752, 819, 995
BfoI RGCGCY 1 cut(s) 220
BisI GCNGC 1 cut(s) 526
BlpI GCTNAGC 1 cut(s) 695
BlsI GCNGC 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 374, 761
Bme18I GGWCC 3 cut(s) 124, 635, 921
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 3 cut(s) 124, 635, 921
BmiI GGNNCC 3 cut(s) 300, 452, 636
BmrFI CCNGG 2 cut(s) 374, 761
BpiI GAAGAC 2 cut(s) 99, 359
BplI GAGNNNNNCTC 3 cut(s) 848, 880, 974
Bpu1102I GCTNAGC 1 cut(s) 695
BpuEI CTTGAG 1 cut(s) 4
BpuMI CCSGG 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 737
BsaJI CCNNGG 1 cut(s) 373
BsaWI WCCGGW 1 cut(s) 702
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 2 cut(s) 474, 508
Bse3DI GCAATG 2 cut(s) 192, 781
Bse8I GATNNNNATC 1 cut(s) 737
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 1 cut(s) 373
BseGI GGATG 1 cut(s) 730
BseJI GATNNNNATC 1 cut(s) 737
BseMI GCAATG 2 cut(s) 192, 781
BseMII CTCAG 1 cut(s) 382
BseNI ACTGG 2 cut(s) 474, 508
BseRI GAGGAG 1 cut(s) 7
BseSI GKGCMC 2 cut(s) 455, 710
BseXI GCAGC 1 cut(s) 512
BshFI GGCC 3 cut(s) 102, 518, 863
BshNI GGYRCC 1 cut(s) 450
BsiHKAI GWGCWC 2 cut(s) 710, 992
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 3 cut(s) 373, 479, 703
BsmAI GTCTC 2 cut(s) 371, 817
BsnI GGCC 3 cut(s) 102, 518, 863
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 3 cut(s) 455, 710, 992
Bsp143I GATC 6 cut(s) 232, 424, 627, 732, 745, 841
Bsp1720I GCTNAGC 1 cut(s) 695
BspACI CCGC 2 cut(s) 806, 845
BspANI GGCC 3 cut(s) 102, 518, 863
BspCNI CTCAG 1 cut(s) 381
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 3 cut(s) 300, 452, 636
BspPI GGATC 3 cut(s) 227, 753, 836
BspT107I GGYRCC 1 cut(s) 450
BsrDI GCAATG 2 cut(s) 192, 781
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 2 cut(s) 474, 508
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 6 cut(s) 232, 424, 627, 732, 745, 841
BssNAI GTATAC 1 cut(s) 438
Bst1107I GTATAC 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 2 cut(s) 98, 162
Bst6I CTCTTC 1 cut(s) 582
BstC8I GCNNGC 2 cut(s) 480, 893
BstDEI CTNAG 2 cut(s) 368, 695
BstF5I GGATG 1 cut(s) 730
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 6 cut(s) 235, 427, 630, 735, 748, 844
BstMAI GTCTC 2 cut(s) 371, 817
BstMBI GATC 6 cut(s) 232, 424, 627, 732, 745, 841
BstMWI GCNNNNNNNGC 5 cut(s) 225, 544, 553, 705, 912
BstNI CCWGG 1 cut(s) 761
BstNSI RCATGY 2 cut(s) 891, 895
BstSCI CCNGG 2 cut(s) 372, 759
BstSLI GKGCMC 2 cut(s) 455, 710
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 2 cut(s) 99, 359
BstZ17I GTATAC 1 cut(s) 438
BsuRI GGCC 3 cut(s) 102, 518, 863
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 1 cut(s) 730
BtsIMutI CAGTG 3 cut(s) 132, 335, 622
Cac8I GCNNGC 2 cut(s) 480, 893
CciI TCATGA 1 cut(s) 942
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 3 cut(s) 124, 635, 921
CseI GACGC 1 cut(s) 721
Csp6I GTAC 1 cut(s) 577
CviAII CATG 4 cut(s) 631, 888, 892, 943
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 2 cut(s) 368, 695
DpnI GATC 6 cut(s) 234, 426, 629, 734, 747, 843
DpnII GATC 6 cut(s) 232, 424, 627, 732, 745, 841
DrdI GACNNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 795
Ecl136II GAGCTC 1 cut(s) 990
Eco147I AGGCCT 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 992
Eco47I GGWCC 3 cut(s) 124, 635, 921
Eco53kI GAGCTC 1 cut(s) 990
Eco57I CTGAAG 3 cut(s) 482, 644, 707
Eco88I CYCGRG 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 990
EcoO109I RGGNCCY 2 cut(s) 124, 635
EcoRII CCWGG 1 cut(s) 759
EcoT22I ATGCAT 1 cut(s) 893
EcoT38I GRGCYC 1 cut(s) 992
FaeI CATG 4 cut(s) 634, 891, 895, 946
FatI CATG 4 cut(s) 630, 887, 891, 942
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 1 cut(s) 526
FokI GGATG 1 cut(s) 737
FriOI GRGCYC 1 cut(s) 992
Fsp4HI GCNGC 1 cut(s) 526
FspBI CTAG 5 cut(s) 558, 665, 752, 819, 995
GlaI GCGC 1 cut(s) 218
GluI GCNGC 1 cut(s) 526
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 3 cut(s) 102, 518, 863
HapII CCGG 3 cut(s) 373, 479, 703
HgaI GACGC 1 cut(s) 721
HhaI GCGC 1 cut(s) 219
Hin1II CATG 4 cut(s) 634, 891, 895, 946
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 2 cut(s) 183, 432
HindII GTYRAC 2 cut(s) 183, 432
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 8 cut(s) 320, 340, 364, 507, 542, 568, 899, 939
HpaI GTTAAC 1 cut(s) 183
HpaII CCGG 3 cut(s) 373, 479, 703
HphI GGTGA 3 cut(s) 95, 740, 997
Hpy166II GTNNAC 5 cut(s) 183, 432, 438, 620, 708
Hpy188I TCNGA 3 cut(s) 277, 328, 833
Hpy188III TCNNGA 5 cut(s) 21, 839, 854, 856, 943
Hpy8I GTNNAC 5 cut(s) 183, 432, 438, 620, 708
Hpy99I CGWCG 3 cut(s) 190, 715, 935
HpyAV CCTTC 4 cut(s) 115, 682, 853, 956
HpyCH4III ACNGT 2 cut(s) 98, 162
HpyCH4IV ACGT 3 cut(s) 185, 201, 434
HpyCH4V TGCA 7 cut(s) 513, 606, 708, 815, 874, 891, 895
HpyF10VI GCNNNNNNNGC 5 cut(s) 225, 544, 553, 705, 912
HpyF3I CTNAG 2 cut(s) 368, 695
HpySE526I ACGT 3 cut(s) 185, 201, 434
Hsp92II CATG 4 cut(s) 634, 891, 895, 946
HspAI GCGC 1 cut(s) 217
KroI GCCGGC 1 cut(s) 478
KroNI GCCGGC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 424
KspAI GTTAAC 1 cut(s) 183
Kzo9I GATC 6 cut(s) 232, 424, 627, 732, 745, 841
LmnI GCTCC 2 cut(s) 298, 995
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 5 cut(s) 558, 665, 752, 819, 995
MaeII ACGT 3 cut(s) 185, 201, 434
MaeIII GTNAC 2 cut(s) 44, 313
MalI GATC 6 cut(s) 234, 426, 629, 734, 747, 843
MboI GATC 6 cut(s) 232, 424, 627, 732, 745, 841
MboII GAAGA 7 cut(s) 104, 364, 599, 637, 668, 727, 989
MhlI GDGCHC 3 cut(s) 455, 710, 992
MluCI AATT 4 cut(s) 40, 610, 798, 955
MlyI GAGTC 4 cut(s) 314, 334, 373, 893
Mph1103I ATGCAT 1 cut(s) 893
MroNI GCCGGC 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 675
MseI TTAA 4 cut(s) 182, 594, 719, 884
MspA1I CMGCKG 1 cut(s) 460
MspI CCGG 3 cut(s) 373, 479, 703
MspR9I CCNGG 2 cut(s) 374, 761
MvaI CCWGG 1 cut(s) 761
MwoI GCNNNNNNNGC 5 cut(s) 225, 544, 553, 705, 912
NaeI GCCGGC 1 cut(s) 480
NciI CCSGG 1 cut(s) 374
NdeII GATC 6 cut(s) 232, 424, 627, 732, 745, 841
NgoMIV GCCGGC 1 cut(s) 478
NlaIII CATG 4 cut(s) 634, 891, 895, 946
NlaIV GGNNCC 3 cut(s) 300, 452, 636
NmuCI GTSAC 2 cut(s) 44, 313
NsiI ATGCAT 1 cut(s) 893
NspI RCATGY 2 cut(s) 891, 895
PaeI GCATGC 1 cut(s) 895
PaeR7I CTCGAG 1 cut(s) 854
PagI TCATGA 1 cut(s) 942
PceI AGGCCT 1 cut(s) 863
PdiI GCCGGC 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 675
PfeI GAWTC 4 cut(s) 507, 542, 568, 939
PkrI GCNGC 1 cut(s) 527
PleI GAGTC 4 cut(s) 314, 334, 372, 893
PpsI GAGTC 4 cut(s) 314, 334, 372, 893
PpuMI RGGWCCY 2 cut(s) 124, 635
Psp124BI GAGCTC 1 cut(s) 992
Psp5II RGGWCCY 2 cut(s) 124, 635
Psp6I CCWGG 1 cut(s) 759
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 3 cut(s) 300, 452, 636
PspPI GGNCC 3 cut(s) 124, 635, 921
PspPPI RGGWCCY 2 cut(s) 124, 635
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SacI GAGCTC 1 cut(s) 992
SaqAI TTAA 4 cut(s) 182, 594, 719, 884
SatI GCNGC 1 cut(s) 526
Sau3AI GATC 6 cut(s) 232, 424, 627, 732, 745, 841
Sau96I GGNCC 3 cut(s) 124, 635, 921
SchI GAGTC 4 cut(s) 314, 334, 373, 893
ScrFI CCNGG 2 cut(s) 374, 761
SduI GDGCHC 3 cut(s) 455, 710, 992
Sfr274I CTCGAG 1 cut(s) 854
SinI GGWCC 3 cut(s) 124, 635, 921
SlaI CTCGAG 1 cut(s) 854
SmlI CTYRAG 2 cut(s) 19, 854
SmoI CTYRAG 2 cut(s) 19, 854
SpeI ACTAGT 1 cut(s) 664
SphI GCATGC 1 cut(s) 895
Sse9I AATT 4 cut(s) 40, 610, 798, 955
SseBI AGGCCT 1 cut(s) 863
SsiI CCGC 2 cut(s) 806, 845
SspMI CTAG 5 cut(s) 558, 665, 752, 819, 995
SstI GAGCTC 1 cut(s) 992
StuI AGGCCT 1 cut(s) 863
StyD4I CCNGG 2 cut(s) 372, 759
TaaI ACNGT 2 cut(s) 98, 162
TaiI ACGT 3 cut(s) 188, 204, 437
TaqI TCGA 5 cut(s) 168, 263, 795, 840, 855
TasI AATT 4 cut(s) 40, 610, 798, 955
TfiI GAWTC 4 cut(s) 507, 542, 568, 939
Tru1I TTAA 4 cut(s) 182, 594, 719, 884
Tru9I TTAA 4 cut(s) 182, 594, 719, 884
TscAI CASTG 3 cut(s) 139, 342, 622
TseFI GTSAC 2 cut(s) 44, 313
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 2 cut(s) 44, 313
TspDTI ATGAA 4 cut(s) 271, 284, 348, 727
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 3 cut(s) 139, 342, 622
VneI GTGCAC 1 cut(s) 706
VpaK11BI GGWCC 3 cut(s) 124, 635, 921
XapI RAATTY 2 cut(s) 610, 798
XceI RCATGY 2 cut(s) 891, 895
XhoI CTCGAG 1 cut(s) 854
XmiI GTMKAC 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 5 cut(s) 558, 665, 752, 819, 995
Zsp2I ATGCAT 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.