Rroxscaffold_4G00281440

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001452
Physical Location & Seq
Forward (+)
3756203 .. 3758330
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281440.1

Sequence Viewer

Length: 921 bp
ATGGAGGACTGTGTCTCTGCTGTGAGGAGATGGGAGGACTTGGAGACGGATATTTTGGTGAAGATTTTTCAGTCGTTTGATGTGTTTGAGTTAACTTCCGGAATAGCTCATGTTTGTAGTGGGTGGCGTTTGGCGTGCTGTGATCCTCTGCTGTGGAAGACGTTGGATTTGTCGATGATGAAATCTAATTTCATCAAAATCCCGTCAGAGCCTTATGTATATGTGGACGACCCGTCCGATAAGCTTTTGTCTCGTTTGTTGAAGATTTCAATGAGTCTCAGCAGGGGAAACATCACGACCTTGATTTTTCATTTCAACTTATATGTGAGCGATGATCAGTTGACTTATACTGCTGAAAGGTGCCCAAAGCTGAAACGACTAGTTTTGCCTGGTTGGAACAGAATAAAAAAGACAGGAATCTGCAAGGCCATTTCCTGCTGGAAGGATCTTGAATCCTTGACAATGCCTAGCATAGCGAATCCCCCCCCATACTTCATAGAAGAGATTGCCAAGAACTGCAGGAACTTCAGAGAACTGAAGATCATGGGTCCTTGTGATATCTTTTTTGCTTCAACCCTAGTTGCATATCTTCCTAAGCTGAAGGTTTTAAGCCTCCGCTGCTCAATGCTCCTAAGGGAGGCTCTACTCATGATCTTGGAGGGCCTACAACAGCTAGAAGTTCTGAATATTTCGCATTGCCTTCTTTTCGACCTCTCTCCATCGCCTACATCTAAAAAAATTTTGAGAGAGATCGATCCTTTTATCCATGAGAAGGCCTCTCGACTACGCAGGTTTTTGACTTGCAGTCAAGACACCTGCGTGATGTGCCAAAGGACGAAAACCGACGAGGGGCTCTTGAGGTGGTACAAGTATGAAGAAGGGCTATGGAAAGAAGACGAGGTGAGCTCCCTTGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

306

Amino Acids

35.36

Weight (kDa)

7.87

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 16 - 57 1.3e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 824
Acc36I ACCTGC 2 cut(s) 780, 824
AccB1I GGYRCC 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 98
AciI CCGC 1 cut(s) 616
AclWI GGATC 3 cut(s) 137, 453, 749
AcsI RAATTY 1 cut(s) 738
AcuI CTGAAG 3 cut(s) 511, 557, 620
AfaI GTAC 1 cut(s) 866
AfiI CCNNNNNNNGG 3 cut(s) 637, 772, 849
AgsI TTSAA 5 cut(s) 262, 270, 316, 452, 573
AhdI GACNNNNNGTC 2 cut(s) 232, 804
AhlI ACTAGT 1 cut(s) 379
AjnI CCWGG 1 cut(s) 388
AleI CACNNNNGTG 1 cut(s) 818
AluBI AGCT 6 cut(s) 107, 244, 370, 598, 673, 906
AluI AGCT 6 cut(s) 107, 244, 370, 598, 673, 906
Alw21I GWGCWC 1 cut(s) 908
Alw26I GTCTC 4 cut(s) 19, 38, 255, 281
AlwI GGATC 3 cut(s) 137, 453, 749
Aor13HI TCCGGA 1 cut(s) 98
AoxI GGCC 3 cut(s) 426, 661, 774
ApeKI GCWGC 1 cut(s) 618
ApoI RAATTY 1 cut(s) 738
ArsI GACNNNNNNTTYG 4 cut(s) 37, 69, 151, 183
AspS9I GGNCC 2 cut(s) 548, 661
AsuHPI GGTGA 2 cut(s) 70, 913
AvaII GGWCC 1 cut(s) 548
AxyI CCTNAGG 1 cut(s) 632
BaeGI GKGCMC 1 cut(s) 365
BanI GGYRCC 1 cut(s) 360
BanII GRGCYC 2 cut(s) 855, 908
BbsI GAAGAC 2 cut(s) 164, 900
Bbv12I GWGCWC 1 cut(s) 908
BbvI GCAGC 1 cut(s) 605
BccI CCATC 2 cut(s) 24, 727
BciT130I CCWGG 1 cut(s) 390
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 4 cut(s) 19, 38, 255, 281
BcuI ACTAGT 1 cut(s) 379
BfaI CTAG 4 cut(s) 380, 468, 578, 674
BfmI CTRYAG 1 cut(s) 517
BfuAI ACCTGC 2 cut(s) 780, 824
BisI GCNGC 1 cut(s) 619
BlsI GCNGC 1 cut(s) 620
Bme1390I CCNGG 1 cut(s) 390
Bme18I GGWCC 1 cut(s) 548
BmeRI GACNNNNNGTC 2 cut(s) 232, 804
BmgT120I GGNCC 2 cut(s) 548, 661
BmiI GGNNCC 2 cut(s) 362, 549
BmrFI CCNGG 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 164, 900
BplI GAGNNNNNCTC 3 cut(s) 761, 793, 890
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 877
Bsa29I ATCGAT 1 cut(s) 753
BsaWI WCCGGW 1 cut(s) 98
Bsc4I CCNNNNNNNGG 3 cut(s) 637, 772, 849
Bse21I CCTNAGG 1 cut(s) 632
Bse3DI GCAATG 1 cut(s) 694
BseAI TCCGGA 1 cut(s) 98
BseBI CCWGG 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 753
BseLI CCNNNNNNNGG 3 cut(s) 637, 772, 849
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 40
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 1 cut(s) 605
BshFI GGCC 3 cut(s) 428, 663, 776
BshNI GGYRCC 1 cut(s) 360
BshVI ATCGAT 1 cut(s) 753
BsiHKAI GWGCWC 1 cut(s) 908
BsiSI CCGG 1 cut(s) 99
BslI CCNNNNNNNGG 3 cut(s) 637, 772, 849
BsmAI GTCTC 4 cut(s) 19, 38, 255, 281
BsmBI CGTCTC 1 cut(s) 38
BsnI GGCC 3 cut(s) 428, 663, 776
Bsp1286I GDGCHC 3 cut(s) 365, 855, 908
Bsp13I TCCGGA 1 cut(s) 98
Bsp143I GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
BspACI CCGC 1 cut(s) 616
BspANI GGCC 3 cut(s) 428, 663, 776
BspCNI CTCAG 1 cut(s) 291
BspDI ATCGAT 1 cut(s) 753
BspEI TCCGGA 1 cut(s) 98
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 2 cut(s) 362, 549
BspMAI CTGCAG 1 cut(s) 521
BspMI ACCTGC 2 cut(s) 780, 824
BspPI GGATC 3 cut(s) 137, 453, 749
BspT107I GGYRCC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 694
BssMI GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 495
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 3 cut(s) 278, 594, 632
BstENI CCTNNNNNAGG 1 cut(s) 635
BstKTI GATC 7 cut(s) 145, 337, 448, 543, 654, 753, 757
BstMAI GTCTC 4 cut(s) 19, 38, 255, 281
BstMBI GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
BstMWI GCNNNNNNNGC 2 cut(s) 618, 825
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 517
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 2 cut(s) 164, 900
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
Bsu15I ATCGAT 1 cut(s) 753
Bsu36I CCTNAGG 1 cut(s) 632
BsuRI GGCC 3 cut(s) 428, 663, 776
BsuTUI ATCGAT 1 cut(s) 753
BtgZI GCGATG 2 cut(s) 345, 705
BveI ACCTGC 2 cut(s) 780, 824
Cac8I GCNNGC 1 cut(s) 136
CciI TCATGA 1 cut(s) 648
Cfr13I GGNCC 2 cut(s) 548, 661
ClaI ATCGAT 1 cut(s) 753
Csp6I GTAC 1 cut(s) 865
CviAII CATG 4 cut(s) 110, 544, 649, 767
CviQI GTAC 1 cut(s) 865
DdeI CTNAG 3 cut(s) 278, 594, 632
DpnI GATC 7 cut(s) 144, 336, 447, 542, 653, 752, 756
DpnII GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
DriI GACNNNNNGTC 2 cut(s) 232, 804
Eam1104I CTCTTC 1 cut(s) 495
Eam1105I GACNNNNNGTC 2 cut(s) 232, 804
EarI CTCTTC 1 cut(s) 495
Ecl136II GAGCTC 1 cut(s) 906
Eco147I AGGCCT 1 cut(s) 776
Eco24I GRGCYC 2 cut(s) 855, 908
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 548
Eco53kI GAGCTC 1 cut(s) 906
Eco57I CTGAAG 3 cut(s) 511, 557, 620
Eco81I CCTNAGG 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 906
EcoNI CCTNNNNNAGG 1 cut(s) 635
EcoO109I RGGNCCY 2 cut(s) 548, 661
EcoRII CCWGG 1 cut(s) 388
EcoRV GATATC 1 cut(s) 559
EcoT38I GRGCYC 2 cut(s) 855, 908
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 4 cut(s) 113, 547, 652, 770
FatI CATG 4 cut(s) 109, 543, 648, 766
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 619
FriOI GRGCYC 2 cut(s) 855, 908
Fsp4HI GCNGC 1 cut(s) 619
FspBI CTAG 4 cut(s) 380, 468, 578, 674
GluI GCNGC 1 cut(s) 619
HaeIII GGCC 3 cut(s) 428, 663, 776
HapII CCGG 1 cut(s) 99
Hin1II CATG 4 cut(s) 113, 547, 652, 770
HincII GTYRAC 2 cut(s) 93, 342
HindII GTYRAC 2 cut(s) 93, 342
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 4 cut(s) 274, 417, 452, 478
HpaI GTTAAC 1 cut(s) 93
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 70, 913
Hpy166II GTNNAC 3 cut(s) 93, 226, 342
Hpy188I TCNGA 4 cut(s) 208, 238, 530, 684
Hpy188III TCNNGA 7 cut(s) 99, 295, 449, 649, 780, 809, 856
Hpy8I GTNNAC 3 cut(s) 93, 226, 342
Hpy99I CGWCG 1 cut(s) 848
HpyAV CCTTC 5 cut(s) 436, 595, 710, 766, 872
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 4 cut(s) 423, 519, 584, 804
HpyF10VI GCNNNNNNNGC 2 cut(s) 618, 825
HpyF3I CTNAG 3 cut(s) 278, 594, 632
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 4 cut(s) 113, 547, 652, 770
Kpn2I TCCGGA 1 cut(s) 98
Ksp22I TGATCA 1 cut(s) 334
KspAI GTTAAC 1 cut(s) 93
Kzo9I GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
LmnI GCTCC 2 cut(s) 633, 911
Lsp1109I GCAGC 1 cut(s) 605
MaeI CTAG 4 cut(s) 380, 468, 578, 674
MaeII ACGT 1 cut(s) 161
MalI GATC 7 cut(s) 144, 336, 447, 542, 653, 752, 756
MboI GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
MboII GAAGA 8 cut(s) 73, 169, 274, 512, 550, 581, 887, 905
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 3 cut(s) 365, 855, 908
MluCI AATT 2 cut(s) 187, 738
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 144, 374
MroI TCCGGA 1 cut(s) 98
MseI TTAA 2 cut(s) 92, 608
MslI CAYNNNNRTG 1 cut(s) 818
MspA1I CMGCKG 1 cut(s) 618
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 390
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 618, 825
NdeII GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
NlaIII CATG 4 cut(s) 113, 547, 652, 770
NlaIV GGNNCC 2 cut(s) 362, 549
OliI CACNNNNGTG 1 cut(s) 818
PagI TCATGA 1 cut(s) 648
PaqCI CACCTGC 1 cut(s) 824
PceI AGGCCT 1 cut(s) 776
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 3 cut(s) 417, 452, 478
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 1 cut(s) 620
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 548
Psp124BI GAGCTC 1 cut(s) 908
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 362, 549
PspPI GGNCC 2 cut(s) 548, 661
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 445
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 1 cut(s) 866
RsaNI GTAC 1 cut(s) 865
RseI CAYNNNNRTG 1 cut(s) 818
SacI GAGCTC 1 cut(s) 908
SaqAI TTAA 2 cut(s) 92, 608
SatI GCNGC 1 cut(s) 619
Sau3AI GATC 7 cut(s) 142, 334, 445, 540, 651, 750, 754
Sau96I GGNCC 2 cut(s) 548, 661
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 3 cut(s) 365, 855, 908
SfcI CTRYAG 1 cut(s) 517
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 856
SmoI CTYRAG 1 cut(s) 856
SpeI ACTAGT 1 cut(s) 379
Sse9I AATT 2 cut(s) 187, 738
SseBI AGGCCT 1 cut(s) 776
SsiI CCGC 1 cut(s) 616
SspI AATATT 1 cut(s) 688
SspMI CTAG 4 cut(s) 380, 468, 578, 674
SstI GAGCTC 1 cut(s) 908
StuI AGGCCT 1 cut(s) 776
StyD4I CCNGG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 164
TaqI TCGA 4 cut(s) 173, 708, 753, 781
TasI AATT 2 cut(s) 187, 738
TfiI GAWTC 3 cut(s) 417, 452, 478
Tru1I TTAA 2 cut(s) 92, 608
Tru9I TTAA 2 cut(s) 92, 608
TseI GCWGC 1 cut(s) 618
TspDTI ATGAA 5 cut(s) 181, 194, 299, 484, 888
TspGWI ACGGA 1 cut(s) 62
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 1 cut(s) 548
XagI CCTNNNNNAGG 1 cut(s) 635
XapI RAATTY 1 cut(s) 738
XspI CTAG 4 cut(s) 380, 468, 578, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.