MD07G1267400.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001452
Physical Location & Seq
Reverse (-)
33536596 .. 33537777
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1267400.v1.1.491

Sequence Viewer

Length: 960 bp
ATGACTTTGGGAAGATGGGAAGACCTGAATGCTGACATATTGTGGAAGATTTTAGACTGCTTCGACGACACCTCTGAATCTGATGATTCATCTTCCGCCATCGGAAAGGTTAACTGTAGTGCCGTTTGCAAGAAGTGGCGTTCGACTCTGTGTGATCCTCGGCTTTGGAACACGCTTGATTTGTCGACGATGAAATCACATTTCATCAAAACCCCAGATAAACCTTATGTTTATGTTTGCAGCCGGTCCGAGATGGCACTGAGTCGCGCTTTGAAGATTTCTCTGAGTCTCAGCCACAGGAACATAACCACGTTGATTTTTAATCTCCAGTTATATGTTCCCAATGATCTCTTCATCTACACCGCTGAAAGGTGCCCGAATCTGAAGCGACTAGTCATGCCATCCTGGAACAAAATAGAGATTGATGGTATCCGCAAGGCCATCGGCTGTTGGAAAGACCTTGAATCCCTGACAATAGGGAACATAGAATGGACCGAATTTCTCGTCCTCTTGAAGGCGATTTCAAACAACTGCAAATATTTCAGCGAGCTAAAATTGATAGGCGCCACGAATCCTACTTGTAACTTTGCTTTAGAACTAGTTAAATATCTTCCAAACCTCAAGGTTTTAAGCCTTCGATGCTCAATGTTCGTGAAGGACGCTTTAACCACGATGCTAGACGGTCTGCAGAACCTGCAAGTTCTCAATGTATCGCATTGCCTTGTTTTGGCTGAAATCCCTCAGCTCGGTCAGGATGTTGTAATTGTTAAAGAGCTTGATCGATCCGTTGTTCACAAGGCTTCGAGGTTACGAAGATTTATAACATGCATGCACATGCAGGACGACGACTCGTCGTGCGTCATGTGCATGAGGAACAGAAACGACGAAGGGATCGTGAGGTGGTATAGATACGAAGAAGGGTTGTGGAAAGAAGATGAGGTGAAATCACTAGCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

320

Amino Acids

36.66

Weight (kDa)

7.44

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 152 - 286 8.2e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
Acc36I ACCTGC 1 cut(s) 702
AccB1I GGYRCC 2 cut(s) 372, 563
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 267
AciI CCGC 3 cut(s) 96, 363, 433
AclWI GGATC 3 cut(s) 149, 777, 899
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 1 cut(s) 404
AcyI GRCGYC 1 cut(s) 564
AfiI CCNNNNNNNGG 4 cut(s) 369, 514, 727, 746
AgsI TTSAA 4 cut(s) 274, 464, 514, 525
AhdI GACNNNNNGTC 1 cut(s) 850
AhlI ACTAGT 2 cut(s) 391, 598
AjnI CCWGG 1 cut(s) 404
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 3 cut(s) 550, 745, 775
AluI AGCT 3 cut(s) 550, 745, 775
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 3 cut(s) 149, 777, 899
AlwNI CAGNNNCTG 1 cut(s) 694
AoxI GGCC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 497
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
AspLEI GCGC 2 cut(s) 269, 566
AspS9I GGNCC 2 cut(s) 246, 492
AsuHPI GGTGA 1 cut(s) 952
AvaII GGWCC 2 cut(s) 246, 492
BaeGI GKGCMC 1 cut(s) 377
BanI GGYRCC 2 cut(s) 372, 563
BbsI GAAGAC 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 1 cut(s) 252
BccI CCATC 6 cut(s) 9, 107, 247, 409, 419, 449
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 1 cut(s) 406
BciVI GTATCC 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 293
BcuI ACTAGT 2 cut(s) 391, 598
BfaI CTAG 4 cut(s) 392, 599, 677, 950
BfmI CTRYAG 2 cut(s) 115, 686
BfoI RGCGCY 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 702
BfuI GTATCC 1 cut(s) 440
BisI GCNGC 1 cut(s) 241
BlsI GCNGC 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 2 cut(s) 246, 492
BmeRI GACNNNNNGTC 1 cut(s) 850
BmgT120I GGNCC 2 cut(s) 246, 492
BmiI GGNNCC 2 cut(s) 374, 565
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 629, 663
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 781
BsaHI GRCGYC 1 cut(s) 564
BsaJI CCNNGG 1 cut(s) 158
Bsc4I CCNNNNNNNGG 4 cut(s) 369, 514, 727, 746
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 328
Bse3DI GCAATG 1 cut(s) 715
BseBI CCWGG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 781
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 2 cut(s) 401, 760
BseLI CCNNNNNNNGG 4 cut(s) 369, 514, 727, 746
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 4 cut(s) 251, 275, 304, 755
BseNI ACTGG 1 cut(s) 328
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 440
BshNI GGYRCC 2 cut(s) 372, 563
BshVI ATCGAT 1 cut(s) 781
BsiSI CCGG 1 cut(s) 244
BslI CCNNNNNNNGG 4 cut(s) 369, 514, 727, 746
BsmAI GTCTC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 377
Bsp143I GATC 5 cut(s) 154, 346, 778, 782, 891
BspACI CCGC 3 cut(s) 96, 363, 433
BspANI GGCC 1 cut(s) 440
BspCNI CTCAG 4 cut(s) 252, 276, 303, 754
BspDI ATCGAT 1 cut(s) 781
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 374, 565
BspMAI CTGCAG 1 cut(s) 690
BspMI ACCTGC 1 cut(s) 702
BspPI GGATC 3 cut(s) 149, 777, 899
BspT107I GGYRCC 2 cut(s) 372, 563
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 328
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 5 cut(s) 154, 346, 778, 782, 891
BssNI GRCGYC 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 406
Bst4CI ACNGT 2 cut(s) 116, 683
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 1 cut(s) 564
BstAPI GCANNNNNTGC 1 cut(s) 694
BstC8I GCNNGC 2 cut(s) 548, 830
BstDEI CTNAG 4 cut(s) 260, 284, 290, 741
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 2 cut(s) 401, 760
BstFNI CGCG 1 cut(s) 267
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 2 cut(s) 269, 566
BstKTI GATC 5 cut(s) 157, 349, 781, 785, 894
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 5 cut(s) 154, 346, 778, 782, 891
BstMWI GCNNNNNNNGC 3 cut(s) 639, 694, 864
BstNI CCWGG 1 cut(s) 406
BstNSI RCATGY 3 cut(s) 828, 832, 838
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 2 cut(s) 115, 686
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 27
Bsu15I ATCGAT 1 cut(s) 781
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 1 cut(s) 440
BsuTUI ATCGAT 1 cut(s) 781
BtsCI GGATG 2 cut(s) 401, 760
BtsIMutI CAGTG 1 cut(s) 257
BveI ACCTGC 1 cut(s) 702
Cac8I GCNNGC 2 cut(s) 548, 830
CaiI CAGNNNCTG 1 cut(s) 694
CfoI GCGC 2 cut(s) 269, 566
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 246, 492
ClaI ATCGAT 1 cut(s) 781
CpoI CGGWCCG 1 cut(s) 246
CseI GACGC 2 cut(s) 668, 847
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 6 cut(s) 397, 825, 829, 835, 862, 868
DdeI CTNAG 4 cut(s) 260, 284, 290, 741
DinI GGCGCC 1 cut(s) 565
DpnI GATC 5 cut(s) 156, 348, 780, 784, 893
DpnII GATC 5 cut(s) 154, 346, 778, 782, 891
DriI GACNNNNNGTC 1 cut(s) 850
Eam1104I CTCTTC 1 cut(s) 356
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 1 cut(s) 85
Eco47I GGWCC 2 cut(s) 246, 492
Eco57I CTGAAG 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 404
EcoT22I ATGCAT 1 cut(s) 830
EgeI GGCGCC 1 cut(s) 565
EheI GGCGCC 1 cut(s) 565
FaeI CATG 6 cut(s) 400, 828, 832, 838, 865, 871
FatI CATG 6 cut(s) 396, 824, 828, 834, 861, 867
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 1 cut(s) 241
FokI GGATG 2 cut(s) 388, 767
Fsp4HI GCNGC 1 cut(s) 241
FspBI CTAG 4 cut(s) 392, 599, 677, 950
GlaI GCGC 2 cut(s) 268, 565
GluI GCNGC 1 cut(s) 241
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 1 cut(s) 440
HapII CCGG 1 cut(s) 244
HgaI GACGC 2 cut(s) 668, 847
HhaI GCGC 2 cut(s) 269, 566
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 6 cut(s) 400, 828, 832, 838, 865, 871
Hin6I GCGC 2 cut(s) 267, 564
HinP1I GCGC 2 cut(s) 267, 564
HincII GTYRAC 2 cut(s) 112, 186
HindII GTYRAC 2 cut(s) 112, 186
HinfI GANTC 9 cut(s) 77, 86, 145, 262, 286, 379, 464, 571, 848
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 952
Hpy166II GTNNAC 3 cut(s) 112, 186, 793
Hpy188I TCNGA 6 cut(s) 76, 82, 104, 250, 285, 384
Hpy188III TCNNGA 4 cut(s) 511, 652, 752, 895
Hpy8I GTNNAC 3 cut(s) 112, 186, 793
Hpy99I CGWCG 5 cut(s) 68, 190, 848, 856, 887
HpyAV CCTTC 5 cut(s) 508, 644, 649, 881, 911
HpyCH4III ACNGT 2 cut(s) 116, 683
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 9 cut(s) 129, 240, 534, 688, 697, 828, 832, 838, 867
HpyF10VI GCNNNNNNNGC 3 cut(s) 639, 694, 864
HpyF3I CTNAG 4 cut(s) 260, 284, 290, 741
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 6 cut(s) 400, 828, 832, 838, 865, 871
HspAI GCGC 2 cut(s) 267, 564
KasI GGCGCC 1 cut(s) 563
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 5 cut(s) 154, 346, 778, 782, 891
Lsp1109I GCAGC 1 cut(s) 252
LweI GCATC 2 cut(s) 629, 663
MaeI CTAG 4 cut(s) 392, 599, 677, 950
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 2 cut(s) 581, 807
MalI GATC 5 cut(s) 156, 348, 780, 784, 893
MboI GATC 5 cut(s) 154, 346, 778, 782, 891
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 3 cut(s) 497, 554, 762
Mly113I GGCGCC 1 cut(s) 564
MlyI GAGTC 4 cut(s) 139, 271, 295, 842
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 9 cut(s) 82, 168, 518, 629, 750, 798, 864, 891, 931
Mph1103I ATGCAT 1 cut(s) 830
MseI TTAA 6 cut(s) 111, 321, 603, 629, 665, 768
MslI CAYNNNNRTG 2 cut(s) 833, 866
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 1 cut(s) 406
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 1 cut(s) 406
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 3 cut(s) 639, 694, 864
NarI GGCGCC 1 cut(s) 564
NdeII GATC 5 cut(s) 154, 346, 778, 782, 891
NlaIII CATG 6 cut(s) 400, 828, 832, 838, 865, 871
NlaIV GGNNCC 2 cut(s) 374, 565
NmeAIII GCCGAG 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 830
NspI RCATGY 3 cut(s) 828, 832, 838
PaeI GCATGC 1 cut(s) 832
PcsI WCGNNNNNNNCGW 1 cut(s) 891
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 5 cut(s) 77, 86, 379, 464, 571
PfoI TCCNGGA 1 cut(s) 404
PkrI GCNGC 1 cut(s) 242
PleI GAGTC 4 cut(s) 139, 270, 294, 842
PluTI GGCGCC 1 cut(s) 567
PpsI GAGTC 4 cut(s) 139, 270, 294, 842
PsiI TTATAA 1 cut(s) 821
Psp6I CCWGG 1 cut(s) 404
PspGI CCWGG 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 374, 565
PspPI GGNCC 2 cut(s) 246, 492
PstI CTGCAG 1 cut(s) 690
PstNI CAGNNNCTG 1 cut(s) 694
RseI CAYNNNNRTG 2 cut(s) 833, 866
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SalI GTCGAC 1 cut(s) 184
SaqAI TTAA 6 cut(s) 111, 321, 603, 629, 665, 768
SatI GCNGC 1 cut(s) 241
Sau3AI GATC 5 cut(s) 154, 346, 778, 782, 891
Sau96I GGNCC 2 cut(s) 246, 492
SchI GAGTC 4 cut(s) 139, 271, 295, 842
ScrFI CCNGG 1 cut(s) 406
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 2 cut(s) 629, 663
SfcI CTRYAG 2 cut(s) 115, 686
SfoI GGCGCC 1 cut(s) 565
SinI GGWCC 2 cut(s) 246, 492
SmiMI CAYNNNNRTG 2 cut(s) 833, 866
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
SpeI ACTAGT 2 cut(s) 391, 598
SphI GCATGC 1 cut(s) 832
Sse9I AATT 3 cut(s) 497, 554, 762
SsiI CCGC 3 cut(s) 96, 363, 433
SspDI GGCGCC 1 cut(s) 563
SspI AATATT 1 cut(s) 539
SspMI CTAG 4 cut(s) 392, 599, 677, 950
StyD4I CCNGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 116, 683
TaiI ACGT 1 cut(s) 314
TaqI TCGA 6 cut(s) 63, 143, 185, 637, 781, 803
TaqII GACCGA 2 cut(s) 509, 737
TasI AATT 3 cut(s) 497, 554, 762
TfiI GAWTC 5 cut(s) 77, 86, 379, 464, 571
Tru1I TTAA 6 cut(s) 111, 321, 603, 629, 665, 768
Tru9I TTAA 6 cut(s) 111, 321, 603, 629, 665, 768
TscAI CASTG 1 cut(s) 264
TseI GCWGC 1 cut(s) 240
TspDTI ATGAA 4 cut(s) 78, 193, 206, 343
TspGWI ACGGA 1 cut(s) 775
TspRI CASTG 1 cut(s) 264
VpaK11BI GGWCC 2 cut(s) 246, 492
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 1 cut(s) 497
XceI RCATGY 3 cut(s) 828, 832, 838
XmiI GTMKAC 1 cut(s) 185
XspI CTAG 4 cut(s) 392, 599, 677, 950
Zsp2I ATGCAT 1 cut(s) 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.