pycom01g20960

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001452
Physical Location & Seq
Reverse (-)
19220951 .. 19223265
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20960.1

Sequence Viewer

Length: 1005 bp
ATGACTGATAAACCCTCCTCAAGAACCAGAACTCTAATAAATTGTGATGGAGTAAGGCTGTTTGGAGGTGGTTGGGAAGCTGGTGAAATGGAAGACGGTGGCTTCCCTGTGAGAAAATGGGAGGACCTGAATATTGACATACTGGTGAGGATTTTTCAGTCTTTCGACATCTTTGAGTTAACGTCGGGCATTGCTCACGTTTGTAGTGCGTGGCGCTTGGCTTGCTGTGATCCTCTGCTGTGGAAAAAGCTTGATTTGTCGATGTTGAAATCGAATTTCATCAAAATACCATTGGAGCCTTATGTCTATGTGGATGGCCGCTCCGATAAGACATTGACTCGTATTTTGAAGACTTCATTGAGTCTCAGCAAGGGAAACATAATGACATTGGTTTTCCATTTCAACTTATTTGTGAGCGATGATCAGTTGACGTATACTGCTGAAAGGTGCCCACAGCTGAAGCGACTGGTTATGCCGGCTTGGAACAGAATAAAAAAGACTGGAATCTGCAAGGCCATTCGCTGCTGGAAAGAGCTTGAATCGCTAACAATGCCTAGCATAGCGAATCCCCCGTACCTCTTGGAAGAGATAAAAAGGAACTGCAAAAATTTCAGTGAACTGAAGATCATGGGTCCTTGTGATATTTTCTTTGCTTCAACACTAGTTGAACATCTTCCAAAACTGAAGGTGCTAAGCCTCCGGTGCTCGACGCTCTTAAAGGATGTTTTGATCATGATCTTGGATCGCCTAGAACACCTAGAAGTTCTCAACATCTCACACTGCCTTCTAATCGAAACCCCTCCGCCTCCTGCAACTAGGAGACTTGTCAGACAACTCGATCCGCTTATTCTCGAGAAGGCCTCTCGGTTGCAAAGATTTTTAACATGCATGCAGGACTCTTGCGTGATGTGCCAAAGGACCAAAAACGACGAGGGAATCATGAGGTGGTATAAGTACGAAGAAGGGCTATGGAAAGAAGATGAGGTGAGCTCCCTAGCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

335

Amino Acids

38.51

Weight (kDa)

8.44

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 46 - 86 4.3e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 447
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 434
AciI CCGC 3 cut(s) 319, 803, 842
AclWI GGATC 3 cut(s) 224, 750, 833
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 2 cut(s) 274, 607
AcuI CTGAAG 3 cut(s) 479, 641, 704
AfaI GTAC 2 cut(s) 575, 956
AgsI TTSAA 6 cut(s) 268, 349, 403, 539, 657, 668
AhlI ACTAGT 1 cut(s) 661
AluBI AGCT 6 cut(s) 80, 250, 457, 535, 990, 998
AluI AGCT 6 cut(s) 80, 250, 457, 535, 990, 998
Alw21I GWGCWC 2 cut(s) 707, 992
Alw26I GTCTC 2 cut(s) 368, 814
AlwI GGATC 3 cut(s) 224, 750, 833
Ama87I CYCGRG 1 cut(s) 851
AoxI GGCC 3 cut(s) 316, 513, 858
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 2 cut(s) 274, 607
ArsI GACNNNNNNTTYG 2 cut(s) 328, 360
Asp700I GAANNNNTTC 1 cut(s) 672
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 3 cut(s) 124, 632, 918
AsuHPI GGTGA 3 cut(s) 95, 157, 997
AvaI CYCGRG 1 cut(s) 851
AvaII GGWCC 3 cut(s) 124, 632, 918
BaeGI GKGCMC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 447
BanII GRGCYC 1 cut(s) 992
BbsI GAAGAC 2 cut(s) 99, 356
Bbv12I GWGCWC 2 cut(s) 707, 992
BbvI GCAGC 1 cut(s) 509
BccI CCATC 2 cut(s) 41, 308
BclI TGATCA 2 cut(s) 421, 729
BcoDI GTCTC 2 cut(s) 368, 814
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 6 cut(s) 555, 662, 749, 758, 816, 995
BfoI RGCGCY 1 cut(s) 217
BisI GCNGC 2 cut(s) 319, 523
BlpI GCTNAGC 1 cut(s) 692
BlsI GCNGC 2 cut(s) 320, 524
Bme18I GGWCC 3 cut(s) 124, 632, 918
BmeT110I CYCGRG 1 cut(s) 851
BmgT120I GGNCC 3 cut(s) 124, 632, 918
BmiI GGNNCC 3 cut(s) 297, 449, 633
BpiI GAAGAC 2 cut(s) 99, 356
BplI GAGNNNNNCTC 3 cut(s) 845, 877, 974
Bpu1102I GCTNAGC 1 cut(s) 692
BpuEI CTTGAG 1 cut(s) 4
BsaBI GATNNNNATC 1 cut(s) 734
BsaWI WCCGGW 1 cut(s) 699
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 3 cut(s) 147, 471, 505
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 1 cut(s) 734
BseGI GGATG 2 cut(s) 319, 727
BseJI GATNNNNATC 1 cut(s) 734
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 379
BseNI ACTGG 3 cut(s) 147, 471, 505
BseRI GAGGAG 1 cut(s) 7
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 1 cut(s) 509
BshFI GGCC 3 cut(s) 318, 515, 860
BshNI GGYRCC 1 cut(s) 447
BsiHKAI GWGCWC 2 cut(s) 707, 992
BsiHKCI CYCGRG 1 cut(s) 851
BsiSI CCGG 2 cut(s) 476, 700
BsmAI GTCTC 2 cut(s) 368, 814
BsnI GGCC 3 cut(s) 318, 515, 860
BsoBI CYCGRG 1 cut(s) 851
Bsp1286I GDGCHC 3 cut(s) 452, 707, 992
Bsp143I GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
Bsp1720I GCTNAGC 1 cut(s) 692
BspACI CCGC 3 cut(s) 319, 803, 842
BspANI GGCC 3 cut(s) 318, 515, 860
BspCNI CTCAG 1 cut(s) 378
BspHI TCATGA 2 cut(s) 732, 939
BspLI GGNNCC 3 cut(s) 297, 449, 633
BspPI GGATC 3 cut(s) 224, 750, 833
BspT107I GGYRCC 1 cut(s) 447
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 475
BsrI ACTGG 3 cut(s) 147, 471, 505
BssAI RCCGGY 1 cut(s) 475
BssMI GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
BssNAI GTATAC 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 579
BstC8I GCNNGC 3 cut(s) 223, 477, 890
BstDEI CTNAG 2 cut(s) 365, 692
BstF5I GGATG 2 cut(s) 319, 727
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 7 cut(s) 232, 424, 627, 732, 738, 745, 841
BstMAI GTCTC 2 cut(s) 368, 814
BstMBI GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
BstMWI GCNNNNNNNGC 5 cut(s) 222, 541, 550, 702, 909
BstNSI RCATGY 2 cut(s) 888, 892
BstSLI GKGCMC 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 2 cut(s) 99, 356
BstZ17I GTATAC 1 cut(s) 435
BsuRI GGCC 3 cut(s) 318, 515, 860
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 319, 727
BtsI GCAGTG 1 cut(s) 778
BtsIMutI CAGTG 2 cut(s) 619, 778
Cac8I GCNNGC 3 cut(s) 223, 477, 890
CciI TCATGA 2 cut(s) 732, 939
CfoI GCGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 3 cut(s) 124, 632, 918
CseI GACGC 1 cut(s) 718
Csp6I GTAC 2 cut(s) 574, 955
CviAII CATG 5 cut(s) 628, 733, 885, 889, 940
CviQI GTAC 2 cut(s) 574, 955
DdeI CTNAG 2 cut(s) 365, 692
DpnI GATC 7 cut(s) 231, 423, 626, 731, 737, 744, 840
DpnII GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
EciI GGCGGA 1 cut(s) 792
Ecl136II GAGCTC 1 cut(s) 990
Eco147I AGGCCT 1 cut(s) 860
Eco24I GRGCYC 1 cut(s) 992
Eco47I GGWCC 3 cut(s) 124, 632, 918
Eco53kI GAGCTC 1 cut(s) 990
Eco57I CTGAAG 3 cut(s) 479, 641, 704
Eco88I CYCGRG 1 cut(s) 851
EcoICRI GAGCTC 1 cut(s) 990
EcoO109I RGGNCCY 2 cut(s) 124, 632
EcoT22I ATGCAT 1 cut(s) 890
EcoT38I GRGCYC 1 cut(s) 992
FaeI CATG 5 cut(s) 631, 736, 888, 892, 943
FatI CATG 5 cut(s) 627, 732, 884, 888, 939
FbaI TGATCA 2 cut(s) 421, 729
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 2 cut(s) 319, 523
FokI GGATG 2 cut(s) 326, 734
FriOI GRGCYC 1 cut(s) 992
Fsp4HI GCNGC 2 cut(s) 319, 523
FspBI CTAG 6 cut(s) 555, 662, 749, 758, 816, 995
GlaI GCGC 1 cut(s) 215
GluI GCNGC 2 cut(s) 319, 523
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 3 cut(s) 318, 515, 860
HapII CCGG 2 cut(s) 476, 700
HgaI GACGC 1 cut(s) 718
HhaI GCGC 1 cut(s) 216
Hin1II CATG 5 cut(s) 631, 736, 888, 892, 943
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HincII GTYRAC 2 cut(s) 180, 429
HindII GTYRAC 2 cut(s) 180, 429
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 7 cut(s) 337, 361, 504, 539, 565, 896, 936
HpaI GTTAAC 1 cut(s) 180
HpaII CCGG 2 cut(s) 476, 700
HphI GGTGA 3 cut(s) 95, 157, 997
Hpy166II GTNNAC 4 cut(s) 180, 429, 435, 617
Hpy188I TCNGA 2 cut(s) 325, 830
Hpy188III TCNNGA 5 cut(s) 21, 733, 851, 853, 940
Hpy8I GTNNAC 4 cut(s) 180, 429, 435, 617
Hpy99I CGWCG 3 cut(s) 187, 712, 932
HpyAV CCTTC 4 cut(s) 679, 794, 850, 956
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 3 cut(s) 182, 198, 431
HpyCH4V TGCA 6 cut(s) 510, 603, 812, 871, 888, 892
HpyF10VI GCNNNNNNNGC 5 cut(s) 222, 541, 550, 702, 909
HpyF3I CTNAG 2 cut(s) 365, 692
HpySE526I ACGT 3 cut(s) 182, 198, 431
Hsp92II CATG 5 cut(s) 631, 736, 888, 892, 943
HspAI GCGC 1 cut(s) 214
KroI GCCGGC 1 cut(s) 475
KroNI GCCGGC 1 cut(s) 477
Ksp22I TGATCA 2 cut(s) 421, 729
KspAI GTTAAC 1 cut(s) 180
Kzo9I GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
LmnI GCTCC 3 cut(s) 295, 326, 995
Lsp1109I GCAGC 1 cut(s) 509
MaeI CTAG 6 cut(s) 555, 662, 749, 758, 816, 995
MaeII ACGT 3 cut(s) 182, 198, 431
MalI GATC 7 cut(s) 231, 423, 626, 731, 737, 744, 840
MbiI CCGCTC 1 cut(s) 321
MboI GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
MboII GAAGA 7 cut(s) 104, 361, 596, 634, 665, 971, 989
MhlI GDGCHC 3 cut(s) 452, 707, 992
MluCI AATT 3 cut(s) 40, 274, 607
MlyI GAGTC 3 cut(s) 331, 370, 890
Mph1103I ATGCAT 1 cut(s) 890
MroNI GCCGGC 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 3 cut(s) 179, 716, 881
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 2 cut(s) 476, 700
MwoI GCNNNNNNNGC 5 cut(s) 222, 541, 550, 702, 909
NaeI GCCGGC 1 cut(s) 477
NdeII GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
NgoMIV GCCGGC 1 cut(s) 475
NlaIII CATG 5 cut(s) 631, 736, 888, 892, 943
NlaIV GGNNCC 3 cut(s) 297, 449, 633
NsiI ATGCAT 1 cut(s) 890
NspI RCATGY 2 cut(s) 888, 892
PaeI GCATGC 1 cut(s) 892
PaeR7I CTCGAG 1 cut(s) 851
PagI TCATGA 2 cut(s) 732, 939
PceI AGGCCT 1 cut(s) 860
PdiI GCCGGC 1 cut(s) 477
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 4 cut(s) 504, 539, 565, 936
PkrI GCNGC 2 cut(s) 320, 524
PleI GAGTC 3 cut(s) 331, 369, 890
PpsI GAGTC 3 cut(s) 331, 369, 890
PpuMI RGGWCCY 2 cut(s) 124, 632
Psp124BI GAGCTC 1 cut(s) 992
Psp5II RGGWCCY 2 cut(s) 124, 632
PspN4I GGNNCC 3 cut(s) 297, 449, 633
PspPI GGNCC 3 cut(s) 124, 632, 918
PspPPI RGGWCCY 2 cut(s) 124, 632
PvuII CAGCTG 1 cut(s) 457
RsaI GTAC 2 cut(s) 575, 956
RsaNI GTAC 2 cut(s) 574, 955
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 992
SaqAI TTAA 3 cut(s) 179, 716, 881
SatI GCNGC 2 cut(s) 319, 523
Sau3AI GATC 7 cut(s) 229, 421, 624, 729, 735, 742, 838
Sau96I GGNCC 3 cut(s) 124, 632, 918
SchI GAGTC 3 cut(s) 331, 370, 890
SduI GDGCHC 3 cut(s) 452, 707, 992
Sfr274I CTCGAG 1 cut(s) 851
SinI GGWCC 3 cut(s) 124, 632, 918
SlaI CTCGAG 1 cut(s) 851
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 19, 851
SmoI CTYRAG 2 cut(s) 19, 851
SpeI ACTAGT 1 cut(s) 661
SphI GCATGC 1 cut(s) 892
Sse9I AATT 3 cut(s) 40, 274, 607
SseBI AGGCCT 1 cut(s) 860
SsiI CCGC 3 cut(s) 319, 803, 842
SspI AATATT 1 cut(s) 133
SspMI CTAG 6 cut(s) 555, 662, 749, 758, 816, 995
SstI GAGCTC 1 cut(s) 992
StuI AGGCCT 1 cut(s) 860
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 3 cut(s) 185, 201, 434
TaqI TCGA 7 cut(s) 165, 260, 272, 707, 792, 837, 852
TasI AATT 3 cut(s) 40, 274, 607
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 4 cut(s) 504, 539, 565, 936
Tru1I TTAA 3 cut(s) 179, 716, 881
Tru9I TTAA 3 cut(s) 179, 716, 881
TscAI CASTG 2 cut(s) 619, 785
TseI GCWGC 1 cut(s) 522
TspDTI ATGAA 2 cut(s) 268, 345
TspRI CASTG 2 cut(s) 619, 785
VpaK11BI GGWCC 3 cut(s) 124, 632, 918
XapI RAATTY 2 cut(s) 274, 607
XceI RCATGY 2 cut(s) 888, 892
XhoI CTCGAG 1 cut(s) 851
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 6 cut(s) 555, 662, 749, 758, 816, 995
Zsp2I ATGCAT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.