Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001190

Physical Location & Seq

Genomic Coordinates Forward (+)

34962183 .. 34964962

Gene Structure 2780 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1295000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGGCGAGGCGGAAGAGGAGGCAGTCGGAGTCGGAGTCGGAGGCGCAAGATTTATCTAATAAGACAGTGAAGCGGAAGTTGGAAGAAAAATCGACAACAACTGAAAAGTTAACAGATAATGACGTCACGCATGATGCCATTACACATTCCCCGCCGCCGAATGTTGAGGTCCGTCGGGCAACCTTTGTTGTAGAAAATGCTTCTCTGAGAATGGGCATCGTTCGAAAGAGGTGGAAGATTCTGGATTCAGAGGAAGACGCTGATGCCCTGAAACAAAAGAAAGATGAGAATAATTATCGGCCTGGAGTTGTCTACCAGGCTCTTCGTGCGATTTTTGATAGCAAACTTAATAAAGCTGGTATGGTAGGAGCTGTGTATGTGAAAACAGATAATGGAGTGTTATTTGAGATTAAACCCCACGTTCGTATACCAAGAACGTGTAGGCGCTTTTGTGGAGTCATGCTGGAGTTACTAGATAAAAAATGCATACGGACCAAAGATACAAATGAAATTCTTATGCGTGTCATTGCGGAACCTGTAACTCGATATTTACCGGTCAACTCTCGTGTAGTAGGACTCTCTTATAGTTCAGAAAAAGTGGTTGATATTGATGACTATGTTAATTCTGCCAGCGATGAATTGAATCTTGTTTTTGTGGTGGGTGAACTGGCCCATGGGAAGATCAATGAGTATACAGATGATTTTATATCGGTTTCAAATTATCCGTTGAGTGCAAAAGGCTGCATAGGGTTGATTTGCGAGTCGTTGGAGCATAAATGGAAGATCTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

EMG1

Protein Analysis

264

Amino Acids

29.94

Weight (kDa)

8.68

Isoelectric Point (pI)

43.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMG1	PF03587	62 - 257	1.1e-48	EMG1/NEP1 methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001190)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57000
fragaria_vesca	FvH4_7g03130 FvH4_7g03130
malus_domestica	MD02G1295000.v1.1 MD03G1003900.v1.1 MD07G1030500.v1.1 MD14G1085000.v1.1
prunus_persica	Prupe.1G569200_v2.0.a1 Prupe.7G040700_v2.0.a1 Prupe.7G040700_v2.0.a1
pyrus_communis	pycom02g24800 pycom03g00390 pycom07g02220 pycom11g00350 pycom12g07800 pycom14g07450
rosa_chinensis	RchiOBHm_Chr1g0317791 RchiOBHm_Chr1g0324931 RchiOBHm_Chr2g0125441 RchiOBHm_Chr4g0421871
rosa_laevigata	RLG00000030168
rosa_multiflora	Rmu_co8495253.1_g000001 Rmu_sc0002447.1_g000012 Rmu_sc0004462.1_g000004 Rmu_sc0010303.1_g000001 Rmu_ssc0000240.1_g000047
rosa_roxburghii	Rroxscaffold_159G00433100 Rroxscaffold_1G00009460 Rroxscaffold_2G00079570 Rroxscaffold_2G00079580 Rroxscaffold_2G00127840 Rroxscaffold_3G00221990 Rroxscaffold_4G00325090 Rroxscaffold_4G00328380 Rroxscaffold_5G00346870
rosa_rugosa	Rorug01G0048600 Rorug01G0048700 Rorug01G0048800 Rorug03G0242300 Rorug05G0231300
rosa_samantha	Rh1AG029400 Rh1AG065000 Rh1AG260200 Rh1AG268400 Rh1BG053700 Rh1CG066400 Rh1DG071000 Rh2BG323900 Rh2DG580800 Rh5DG407100 Rh5DG421200
rosa_wichuraiana	Rw1G005440 Rw1G014820 Rw4G005800 Rw5G023610 Rw6G020160 Rw7G010520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	126
AccI	GTMKAC	3 cut(s)	312, 427, 692
AciI	CCGC	5 cut(s)	10, 73, 152, 155, 530
AcsI	RAATTY	1 cut(s)	510
AcyI	GRCGYC	1 cut(s)	123
AflIII	ACRYGT	1 cut(s)	437
AgeI	ACCGGT	1 cut(s)	553
AgsI	TTSAA	2 cut(s)	643, 717
AjnI	CCWGG	2 cut(s)	301, 315
AjuI	GAANNNNNNNTTGG	2 cut(s)	62, 94
AluBI	AGCT	2 cut(s)	356, 371
AluI	AGCT	2 cut(s)	356, 371
AoxI	GGCC	2 cut(s)	299, 669
ApeKI	GCWGC	1 cut(s)	741
ApoI	RAATTY	1 cut(s)	510
AsiGI	ACCGGT	1 cut(s)	553
Asp700I	GAANNNNTTC	1 cut(s)	785
AspLEI	GCGC	2 cut(s)	46, 447
AspS9I	GGNCC	3 cut(s)	169, 492, 670
AsuHPI	GGTGA	1 cut(s)	674
AsuII	TTCGAA	1 cut(s)	223
AvaII	GGWCC	2 cut(s)	169, 492
BauI	CACGAG	1 cut(s)	564
BbsI	GAAGAC	1 cut(s)	261
BbvI	GCAGC	1 cut(s)	728
BciT130I	CCWGG	2 cut(s)	303, 317
BfaI	CTAG	1 cut(s)	473
BfoI	RGCGCY	1 cut(s)	448
BglII	AGATCT	1 cut(s)	783
BisI	GCNGC	2 cut(s)	155, 742
BlsI	GCNGC	2 cut(s)	156, 743
Bme1390I	CCNGG	2 cut(s)	303, 317
Bme18I	GGWCC	2 cut(s)	169, 492
BmgT120I	GGNCC	3 cut(s)	169, 492, 670
BmiI	GGNNCC	1 cut(s)	534
BmrFI	CCNGG	2 cut(s)	303, 317
BmsI	GCATC	3 cut(s)	124, 225, 253
BpiI	GAAGAC	1 cut(s)	261
BpmI	CTGGAG	2 cut(s)	324, 485
Bpu14I	TTCGAA	1 cut(s)	223
BsaHI	GRCGYC	1 cut(s)	123
BsaJI	CCNNGG	1 cut(s)	673
BsaWI	WCCGGW	1 cut(s)	553
BsaXI	ACNNNNNCTCC	2 cut(s)	20, 50
Bse118I	RCCGGY	1 cut(s)	553
Bse1I	ACTGG	1 cut(s)	672
Bse3DI	GCAATG	1 cut(s)	525
BseBI	CCWGG	2 cut(s)	303, 317
BseDI	CCNNGG	1 cut(s)	673
BseMI	GCAATG	1 cut(s)	525
BseMII	CTCAG	1 cut(s)	197
BseNI	ACTGG	1 cut(s)	672
BseRI	GAGGAG	1 cut(s)	31
BseXI	GCAGC	1 cut(s)	728
BshFI	GGCC	2 cut(s)	301, 671
BshTI	ACCGGT	1 cut(s)	553
BsiSI	CCGG	1 cut(s)	554
BsnI	GGCC	2 cut(s)	301, 671
Bsp119I	TTCGAA	1 cut(s)	223
Bsp143I	GATC	2 cut(s)	681, 783
Bsp19I	CCATGG	1 cut(s)	673
BspACI	CCGC	5 cut(s)	10, 73, 152, 155, 530
BspANI	GGCC	2 cut(s)	301, 671
BspCNI	CTCAG	1 cut(s)	198
BspLI	GGNNCC	1 cut(s)	534
BspQI	GCTCTTC	1 cut(s)	327
BspT104I	TTCGAA	1 cut(s)	223
BsrDI	GCAATG	1 cut(s)	525
BsrFI	RCCGGY	1 cut(s)	553
BsrI	ACTGG	1 cut(s)	672
BssAI	RCCGGY	1 cut(s)	553
BssECI	CCNNGG	1 cut(s)	673
BssMI	GATC	2 cut(s)	681, 783
BssNAI	GTATAC	2 cut(s)	428, 693
BssNI	GRCGYC	1 cut(s)	123
BssSI	CACGAG	1 cut(s)	564
BssT1I	CCWWGG	1 cut(s)	673
Bst1107I	GTATAC	2 cut(s)	428, 693
Bst2BI	CACGAG	1 cut(s)	564
Bst2UI	CCWGG	2 cut(s)	303, 317
Bst4CI	ACNGT	1 cut(s)	67
Bst6I	CTCTTC	2 cut(s)	8, 327
BstACI	GRCGYC	1 cut(s)	123
BstBI	TTCGAA	1 cut(s)	223
BstC8I	GCNNGC	1 cut(s)	631
BstDEI	CTNAG	1 cut(s)	206
BstDSI	CCRYGG	1 cut(s)	673
BstH2I	RGCGCY	1 cut(s)	448
BstHHI	GCGC	2 cut(s)	46, 447
BstKTI	GATC	2 cut(s)	684, 786
BstMBI	GATC	2 cut(s)	681, 783
BstMWI	GCNNNNNNNGC	1 cut(s)	326
BstNI	CCWGG	2 cut(s)	303, 317
BstSCI	CCNGG	2 cut(s)	301, 315
BstV1I	GCAGC	1 cut(s)	728
BstV2I	GAAGAC	1 cut(s)	261
BstX2I	RGATCY	1 cut(s)	783
BstYI	RGATCY	1 cut(s)	783
BstZ17I	GTATAC	2 cut(s)	428, 693
BsuRI	GGCC	2 cut(s)	301, 671
BtgI	CCRYGG	1 cut(s)	673
BtgZI	GCGATG	1 cut(s)	648
BtsIMutI	CAGTG	1 cut(s)	72
Cac8I	GCNNGC	1 cut(s)	631
CfoI	GCGC	2 cut(s)	46, 447
Cfr10I	RCCGGY	1 cut(s)	553
Cfr13I	GGNCC	3 cut(s)	169, 492, 670
CseI	GACGC	1 cut(s)	266
CspAI	ACCGGT	1 cut(s)	553
CviAII	CATG	3 cut(s)	131, 460, 674
CviJI	RGCY	6 cut(s)	301, 320, 356, 371, 671, 741
CviKI_1	RGCY	6 cut(s)	301, 320, 356, 371, 671, 741
DdeI	CTNAG	1 cut(s)	206
DpnI	GATC	2 cut(s)	683, 785
DpnII	GATC	2 cut(s)	681, 783
Eam1104I	CTCTTC	2 cut(s)	8, 327
EarI	CTCTTC	2 cut(s)	8, 327
EciI	GGCGGA	1 cut(s)	25
Eco130I	CCWWGG	1 cut(s)	673
Eco47I	GGWCC	2 cut(s)	169, 492
EcoRII	CCWGG	2 cut(s)	301, 315
EcoT14I	CCWWGG	1 cut(s)	673
EcoT22I	ATGCAT	1 cut(s)	488
ErhI	CCWWGG	1 cut(s)	673
FaeI	CATG	3 cut(s)	134, 463, 677
FatI	CATG	3 cut(s)	130, 459, 673
FauI	CCCGC	1 cut(s)	159
FblI	GTMKAC	3 cut(s)	312, 427, 692
Fnu4HI	GCNGC	2 cut(s)	155, 742
Fsp4HI	GCNGC	2 cut(s)	155, 742
FspBI	CTAG	1 cut(s)	473
GlaI	GCGC	2 cut(s)	45, 446
GluI	GCNGC	2 cut(s)	155, 742
GsuI	CTGGAG	2 cut(s)	324, 485
HaeII	RGCGCY	1 cut(s)	448
HaeIII	GGCC	2 cut(s)	301, 671
HapII	CCGG	1 cut(s)	554
HgaI	GACGC	1 cut(s)	266
HhaI	GCGC	2 cut(s)	46, 447
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	3 cut(s)	134, 463, 677
Hin6I	GCGC	2 cut(s)	44, 445
HinP1I	GCGC	2 cut(s)	44, 445
HincII	GTYRAC	2 cut(s)	111, 559
HindII	GTYRAC	2 cut(s)	111, 559
HinfI	GANTC	8 cut(s)	29, 35, 238, 245, 456, 576, 643, 761
HpaI	GTTAAC	1 cut(s)	111
HpaII	CCGG	1 cut(s)	554
HphI	GGTGA	1 cut(s)	674
Hpy166II	GTNNAC	6 cut(s)	111, 313, 428, 559, 665, 693
Hpy188I	TCNGA	7 cut(s)	28, 34, 40, 207, 250, 592, 791
Hpy188III	TCNNGA	1 cut(s)	242
Hpy8I	GTNNAC	6 cut(s)	111, 313, 428, 559, 665, 693
Hpy99I	CGWCG	1 cut(s)	177
HpyCH4III	ACNGT	1 cut(s)	67
HpyCH4IV	ACGT	3 cut(s)	123, 420, 437
HpyCH4V	TGCA	3 cut(s)	486, 734, 744
HpyF10VI	GCNNNNNNNGC	1 cut(s)	326
HpyF3I	CTNAG	1 cut(s)	206
HpySE526I	ACGT	3 cut(s)	123, 420, 437
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	3 cut(s)	134, 463, 677
HspAI	GCGC	2 cut(s)	44, 445
KspAI	GTTAAC	1 cut(s)	111
Kzo9I	GATC	2 cut(s)	681, 783
LguI	GCTCTTC	1 cut(s)	327
LmnI	GCTCC	2 cut(s)	368, 769
Lsp1109I	GCAGC	1 cut(s)	728
LweI	GCATC	3 cut(s)	124, 225, 253
MaeI	CTAG	1 cut(s)	473
MaeII	ACGT	3 cut(s)	123, 420, 437
MaeIII	GTNAC	3 cut(s)	124, 468, 538
MalI	GATC	2 cut(s)	683, 785
MboI	GATC	2 cut(s)	681, 783
MboII	GAAGA	7 cut(s)	25, 95, 247, 266, 314, 691, 778
MflI	RGATCY	1 cut(s)	783
MluCI	AATT	5 cut(s)	292, 510, 622, 638, 718
MlyI	GAGTC	5 cut(s)	38, 44, 465, 570, 770
MmeI	TCCRAC	5 cut(s)	6, 12, 18, 60, 747
MnlI	CCTC	6 cut(s)	9, 12, 34, 160, 222, 244
Mph1103I	ATGCAT	1 cut(s)	488
MroXI	GAANNNNTTC	1 cut(s)	785
MseI	TTAA	4 cut(s)	110, 348, 411, 621
MspI	CCGG	1 cut(s)	554
MspR9I	CCNGG	2 cut(s)	303, 317
MvaI	CCWGG	2 cut(s)	303, 317
MwoI	GCNNNNNNNGC	1 cut(s)	326
NcoI	CCATGG	1 cut(s)	673
NdeII	GATC	2 cut(s)	681, 783
NlaIII	CATG	3 cut(s)	134, 463, 677
NlaIV	GGNNCC	1 cut(s)	534
NmuCI	GTSAC	1 cut(s)	124
NsiI	ATGCAT	1 cut(s)	488
NspV	TTCGAA	1 cut(s)	223
PciSI	GCTCTTC	1 cut(s)	327
PdmI	GAANNNNTTC	1 cut(s)	785
PfeI	GAWTC	3 cut(s)	238, 245, 643
PinAI	ACCGGT	1 cut(s)	553
PkrI	GCNGC	2 cut(s)	156, 743
PleI	GAGTC	5 cut(s)	37, 43, 464, 570, 769
PpsI	GAGTC	5 cut(s)	37, 43, 464, 570, 769
Psp6I	CCWGG	2 cut(s)	301, 315
PspGI	CCWGG	2 cut(s)	301, 315
PspN4I	GGNNCC	1 cut(s)	534
PspPI	GGNCC	3 cut(s)	169, 492, 670
PsuI	RGATCY	1 cut(s)	783
SapI	GCTCTTC	1 cut(s)	327
SaqAI	TTAA	4 cut(s)	110, 348, 411, 621
SatI	GCNGC	2 cut(s)	155, 742
Sau3AI	GATC	2 cut(s)	681, 783
Sau96I	GGNCC	3 cut(s)	169, 492, 670
SchI	GAGTC	5 cut(s)	38, 44, 465, 570, 770
ScrFI	CCNGG	2 cut(s)	303, 317
SetI	ASST	9 cut(s)	126, 171, 185, 233, 358, 373, 423, 440, 538
SfaNI	GCATC	3 cut(s)	124, 225, 253
SfuI	TTCGAA	1 cut(s)	223
SinI	GGWCC	2 cut(s)	169, 492
Sse9I	AATT	5 cut(s)	292, 510, 622, 638, 718
SsiI	CCGC	5 cut(s)	10, 73, 152, 155, 530
SspMI	CTAG	1 cut(s)	473
StyD4I	CCNGG	2 cut(s)	301, 315
StyI	CCWWGG	1 cut(s)	673
TaaI	ACNGT	1 cut(s)	67
TaiI	ACGT	3 cut(s)	126, 423, 440
TaqI	TCGA	3 cut(s)	92, 223, 544
TasI	AATT	5 cut(s)	292, 510, 622, 638, 718
TauI	GCSGC	1 cut(s)	157
TfiI	GAWTC	3 cut(s)	238, 245, 643
Tru1I	TTAA	4 cut(s)	110, 348, 411, 621
Tru9I	TTAA	4 cut(s)	110, 348, 411, 621
TscAI	CASTG	1 cut(s)	72
TseFI	GTSAC	1 cut(s)	124
TseI	GCWGC	1 cut(s)	741
Tsp45I	GTSAC	1 cut(s)	124
TspDTI	ATGAA	2 cut(s)	522, 651
TspGWI	ACGGA	3 cut(s)	161, 505, 714
TspRI	CASTG	1 cut(s)	72
VpaK11BI	GGWCC	2 cut(s)	169, 492
XapI	RAATTY	1 cut(s)	510
XmiI	GTMKAC	3 cut(s)	312, 427, 692
XmnI	GAANNNNTTC	1 cut(s)	785
XspI	CTAG	1 cut(s)	473
ZraI	GACGTC	1 cut(s)	124
Zsp2I	ATGCAT	1 cut(s)	488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.