pycom07g02220

Ribosomal RNA small subunit methyltransferase NEP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001190
Physical Location & Seq
Forward (+)
1800331 .. 1802266
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02220.4

Sequence Viewer

Length: 798 bp
ATGGTGAGGTGGAAGAGAGGGCAGTCGGAGTCGCAGTCGCAGAATTTATCCACTAGGATGGTGAAACGGAAGTTGGAAGACGAATCGACAACAACCGAAGAGAAAACAGAAGATGATGACACGGATAATGCCATTATACCGTCCCCGCCGAATGTTGAGGTCCGGGCAACTTTTATTGTAGAAAATGCTTCTCTGAGGAAAGGCATCGTTCGAAAGAGGTGGAAGATTCTAGATTCAGAGGAAGACGCTGACTTCCTCAAACAAAAGAAAGATGTGAATGATTATAGGACTGGAGTTGTCTACCAGATTTGGTTTATAGGCATGATGTTTCTTTTCACCACTATGGCTCTTCGTTCGATTTTTGATAGCAAACTTAATAAAGCTGGTATGGTACGAGCTGTGTATGTAAAAACAGATAATGGAGTGTTATTTGAGATTAAACCCCATGTCCGTTTACCAAGAACGTGTAGGCTCGTTTTATATTCACCTTTGCACCATTCTGCCTCTTCAAAATGTCATGGAACTTACGAATTGCTTTGTATTTTGACTTCTGCAATTTCTAAAGTGGAGTTACTAGATAAAAAATGCATACGGACCAAAGATACAAATGAAATTCTTATGCGCGTCATTGCGGAACCTCATTCTGAGCTTACTGATGACTATGTTAATTCTGCCAGTGATGAATTGAATCTTGTTTTTGTGGTGGGTGCAGAAACCCATGGGAAGATCAATGAGTATACAGATGATTTTATATCAGGTAATTTGTACTTGAGTTATTTGATAGTATGGTTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EMG1

Protein Analysis

266

Amino Acids

30.47

Weight (kDa)

6.33

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 300, 737
AccII CGCG 1 cut(s) 624
AciI CCGC 2 cut(s) 146, 632
AcsI RAATTY 2 cut(s) 43, 612
AfaI GTAC 2 cut(s) 393, 767
AflIII ACRYGT 1 cut(s) 464
AgsI TTSAA 2 cut(s) 510, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 383, 398, 649
AluI AGCT 3 cut(s) 383, 398, 649
ApoI RAATTY 2 cut(s) 43, 612
AspLEI GCGC 1 cut(s) 624
AspS9I GGNCC 2 cut(s) 160, 594
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 4 cut(s) 16, 73, 328, 477
AsuII TTCGAA 1 cut(s) 211
AvaII GGWCC 2 cut(s) 160, 594
BarI GAAGNNNNNNTAC 2 cut(s) 532, 564
BbsI GAAGAC 2 cut(s) 84, 249
BccI CCATC 1 cut(s) 52
BcnI CCSGG 1 cut(s) 164
BfaI CTAG 3 cut(s) 54, 230, 575
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 160, 594
BmgT120I GGNCC 2 cut(s) 160, 594
BmiI GGNNCC 1 cut(s) 636
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 1 cut(s) 213
BpiI GAAGAC 2 cut(s) 84, 249
BpmI CTGGAG 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bse1I ACTGG 2 cut(s) 295, 675
Bse3DI GCAATG 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 1 cut(s) 63
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 2 cut(s) 185, 636
BseNI ACTGG 2 cut(s) 295, 675
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 1 cut(s) 624
BsiSI CCGG 1 cut(s) 163
BslFI GGGAC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 211
Bsp143I GATC 1 cut(s) 726
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 2 cut(s) 146, 632
BspCNI CTCAG 2 cut(s) 186, 637
BspFNI CGCG 1 cut(s) 624
BspLI GGNNCC 1 cut(s) 636
BspQI GCTCTTC 1 cut(s) 354
BspT104I TTCGAA 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 627
BsrI ACTGG 2 cut(s) 295, 675
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 1 cut(s) 726
BssNAI GTATAC 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 718
Bst1107I GTATAC 1 cut(s) 738
Bst4CI ACNGT 1 cut(s) 141
Bst6I CTCTTC 4 cut(s) 8, 93, 354, 511
BstBI TTCGAA 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 194, 645
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 624
BstHHI GCGC 1 cut(s) 624
BstKTI GATC 1 cut(s) 729
BstMBI GATC 1 cut(s) 726
BstSCI CCNGG 1 cut(s) 162
BstUI CGCG 1 cut(s) 624
BstV2I GAAGAC 2 cut(s) 84, 249
BstXI CCANNNNNNTGG 1 cut(s) 58
BstZ17I GTATAC 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 718
BtsCI GGATG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 682
CfoI GCGC 1 cut(s) 624
Cfr13I GGNCC 2 cut(s) 160, 594
CseI GACGC 2 cut(s) 254, 613
Csp6I GTAC 2 cut(s) 392, 766
CviAII CATG 4 cut(s) 322, 446, 518, 719
CviJI RGCY 5 cut(s) 347, 383, 398, 472, 649
CviKI_1 RGCY 5 cut(s) 347, 383, 398, 472, 649
CviQI GTAC 2 cut(s) 392, 766
DdeI CTNAG 2 cut(s) 194, 645
DpnI GATC 1 cut(s) 728
DpnII GATC 1 cut(s) 726
Eam1104I CTCTTC 4 cut(s) 8, 93, 354, 511
EarI CTCTTC 4 cut(s) 8, 93, 354, 511
Eco130I CCWWGG 1 cut(s) 718
Eco47I GGWCC 2 cut(s) 160, 594
EcoT14I CCWWGG 1 cut(s) 718
EcoT22I ATGCAT 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 4 cut(s) 325, 449, 521, 722
FaqI GGGAC 1 cut(s) 127
FatI CATG 4 cut(s) 321, 445, 517, 718
FauI CCCGC 1 cut(s) 153
FblI GTMKAC 2 cut(s) 300, 737
FokI GGATG 1 cut(s) 70
FspBI CTAG 3 cut(s) 54, 230, 575
GlaI GCGC 1 cut(s) 623
GsuI CTGGAG 1 cut(s) 312
HapII CCGG 1 cut(s) 163
HgaI GACGC 2 cut(s) 254, 613
HhaI GCGC 1 cut(s) 624
Hin1II CATG 4 cut(s) 325, 449, 521, 722
Hin6I GCGC 1 cut(s) 622
HinP1I GCGC 1 cut(s) 622
HinfI GANTC 5 cut(s) 29, 83, 226, 233, 688
HpaII CCGG 1 cut(s) 163
HphI GGTGA 4 cut(s) 16, 73, 328, 477
Hpy166II GTNNAC 3 cut(s) 301, 455, 738
Hpy188I TCNGA 4 cut(s) 28, 195, 238, 646
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 3 cut(s) 301, 455, 738
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4IV ACGT 1 cut(s) 464
HpyCH4V TGCA 4 cut(s) 493, 554, 588, 710
HpyF3I CTNAG 2 cut(s) 194, 645
HpySE526I ACGT 1 cut(s) 464
Hsp92II CATG 4 cut(s) 325, 449, 521, 722
HspAI GCGC 1 cut(s) 622
Kzo9I GATC 1 cut(s) 726
LguI GCTCTTC 1 cut(s) 354
LpnPI CCDG 6 cut(s) 176, 276, 317, 369, 688, 741
LweI GCATC 1 cut(s) 213
MaeI CTAG 3 cut(s) 54, 230, 575
MaeII ACGT 1 cut(s) 464
MaeIII GTNAC 1 cut(s) 570
MalI GATC 1 cut(s) 728
MboI GATC 1 cut(s) 726
MboII GAAGA 9 cut(s) 25, 89, 110, 122, 235, 254, 341, 498, 736
MluCI AATT 7 cut(s) 43, 530, 555, 612, 667, 683, 760
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 6, 54
MnlI CCTC 8 cut(s) 11, 151, 189, 210, 232, 266, 514, 648
Mph1103I ATGCAT 1 cut(s) 590
MseI TTAA 3 cut(s) 375, 438, 666
MslI CAYNNNNRTG 2 cut(s) 56, 341
MspI CCGG 1 cut(s) 163
MspR9I CCNGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 624
NciI CCSGG 1 cut(s) 164
NcoI CCATGG 1 cut(s) 718
NdeII GATC 1 cut(s) 726
NlaIII CATG 4 cut(s) 325, 449, 521, 722
NlaIV GGNNCC 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 590
NspV TTCGAA 1 cut(s) 211
PciSI GCTCTTC 1 cut(s) 354
PfeI GAWTC 4 cut(s) 83, 226, 233, 688
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 636
PspPI GGNCC 2 cut(s) 160, 594
RsaI GTAC 2 cut(s) 393, 767
RsaNI GTAC 2 cut(s) 392, 766
RseI CAYNNNNRTG 2 cut(s) 56, 341
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 3 cut(s) 375, 438, 666
Sau3AI GATC 1 cut(s) 726
Sau96I GGNCC 2 cut(s) 160, 594
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 164
SfaNI GCATC 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 211
SinI GGWCC 2 cut(s) 160, 594
SmiMI CAYNNNNRTG 2 cut(s) 56, 341
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
Sse9I AATT 7 cut(s) 43, 530, 555, 612, 667, 683, 760
SsiI CCGC 2 cut(s) 146, 632
SspMI CTAG 3 cut(s) 54, 230, 575
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 1 cut(s) 141
TaiI ACGT 1 cut(s) 467
TaqI TCGA 3 cut(s) 86, 211, 356
TasI AATT 7 cut(s) 43, 530, 555, 612, 667, 683, 760
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 4 cut(s) 83, 226, 233, 688
Tru1I TTAA 3 cut(s) 375, 438, 666
Tru9I TTAA 3 cut(s) 375, 438, 666
TscAI CASTG 1 cut(s) 682
TspDTI ATGAA 2 cut(s) 624, 696
TspGWI ACGGA 4 cut(s) 82, 137, 440, 607
TspRI CASTG 1 cut(s) 682
VpaK11BI GGWCC 2 cut(s) 160, 594
XapI RAATTY 2 cut(s) 43, 612
XbaI TCTAGA 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 300, 737
XspI CTAG 3 cut(s) 54, 230, 575
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.