MD03G1003900.v1.1

Ribosomal RNA small subunit methyltransferase NEP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001190
Physical Location & Seq
Forward (+)
281689 .. 282877
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1003900.v1.1.491

Sequence Viewer

Length: 336 bp
ATGTTGGAGTTACTAAACGAGAAATGCATACGGACGGAAGATACAAATGAAGTACTTATGCGTGTCATTGAGGAACCTGTAACTCGACGTTTACCTATCAACTCTCGTGTGGTAGGTCTCTCTTATAGTGCAGAGAAGGTGGTTGACATAGATAAGTACGTTAATTCTGCCGGTGTTGATGATCAGGGTCTTGTTTTTGTGGTGGGTGCAATGGCCCATGGAAAAATCAATGAGGAGTACGCAGATGATTTCATATCAGTGTCGAATTATCGATTGGAAGCGAAAAACTGCATAGCATTGATTTGCGAGGCGTTGGAGTATAAATGGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EMG1

Protein Analysis

112

Amino Acids

12.58

Weight (kDa)

4.77

Isoelectric Point (pI)

20.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMG1 PF03587 1 - 106 2.3e-19 EMG1/NEP1 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 3 cut(s) 54, 158, 239
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
Alw26I GTCTC 1 cut(s) 122
AoxI GGCC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 214
BauI CACGAG 1 cut(s) 105
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 122
BglII AGATCT 1 cut(s) 330
BmcAI AGTACT 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 75
Bsa29I ATCGAT 1 cut(s) 271
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 216
BseCI ATCGAT 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 1 cut(s) 215
BshVI ATCGAT 1 cut(s) 271
BsiSI CCGG 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 122
BsnI GGCC 1 cut(s) 215
Bso31I GGTCTC 1 cut(s) 122
Bsp143I GATC 2 cut(s) 181, 330
Bsp19I CCATGG 1 cut(s) 217
BspANI GGCC 1 cut(s) 215
BspDI ATCGAT 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 75
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 181, 330
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 217
Bst2BI CACGAG 1 cut(s) 105
BstDSI CCRYGG 1 cut(s) 217
BstKTI GATC 2 cut(s) 184, 333
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 181, 330
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
Bsu15I ATCGAT 1 cut(s) 271
BsuRI GGCC 1 cut(s) 215
BsuTUI ATCGAT 1 cut(s) 271
BtgI CCRYGG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 214
ClaI ATCGAT 1 cut(s) 271
Csp6I GTAC 3 cut(s) 53, 157, 238
CviAII CATG 1 cut(s) 218
CviJI RGCY 1 cut(s) 215
CviKI_1 RGCY 1 cut(s) 215
CviQI GTAC 3 cut(s) 53, 157, 238
DpnI GATC 2 cut(s) 183, 332
DpnII GATC 2 cut(s) 181, 330
Eco130I CCWWGG 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 217
EcoT22I ATGCAT 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 1 cut(s) 221
FaiI YATR 8 cut(s) 29, 59, 126, 149, 219, 254, 293, 321
FatI CATG 1 cut(s) 217
FbaI TGATCA 1 cut(s) 181
HaeIII GGCC 1 cut(s) 215
HapII CCGG 1 cut(s) 171
Hin1II CATG 1 cut(s) 221
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HpaII CCGG 1 cut(s) 171
Hpy166II GTNNAC 2 cut(s) 92, 145
Hpy188I TCNGA 1 cut(s) 335
Hpy8I GTNNAC 2 cut(s) 92, 145
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 88, 159
HpyCH4V TGCA 4 cut(s) 27, 131, 209, 291
HpySE526I ACGT 2 cut(s) 88, 159
Hsp92II CATG 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 181, 330
LpnPI CCDG 3 cut(s) 90, 170, 184
MaeII ACGT 2 cut(s) 88, 159
MaeIII GTNAC 2 cut(s) 9, 79
MalI GATC 2 cut(s) 183, 332
MboI GATC 2 cut(s) 181, 330
MboII GAAGA 1 cut(s) 50
MflI RGATCY 1 cut(s) 330
MluCI AATT 2 cut(s) 163, 265
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 3 cut(s) 64, 226, 301
Mph1103I ATGCAT 1 cut(s) 29
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 1 cut(s) 171
NcoI CCATGG 1 cut(s) 217
NdeII GATC 2 cut(s) 181, 330
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 75
NsiI ATGCAT 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 1 cut(s) 214
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 3 cut(s) 54, 158, 239
RsaNI GTAC 3 cut(s) 53, 157, 238
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 1 cut(s) 162
Sau3AI GATC 2 cut(s) 181, 330
Sau96I GGNCC 1 cut(s) 214
ScaI AGTACT 1 cut(s) 54
SetI ASST 6 cut(s) 79, 91, 97, 118, 141, 162
SmiMI CAYNNNNRTG 1 cut(s) 257
Sse9I AATT 2 cut(s) 163, 265
StyI CCWWGG 1 cut(s) 217
TaiI ACGT 2 cut(s) 91, 162
TaqI TCGA 3 cut(s) 85, 263, 271
TasI AATT 2 cut(s) 163, 265
TatI WGTACW 1 cut(s) 52
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 1 cut(s) 264
TspDTI ATGAA 2 cut(s) 63, 241
TspGWI ACGGA 2 cut(s) 46, 50
TspRI CASTG 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.