pycom02g24800

Ribosomal RNA small subunit methyltransferase NEP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001190
Physical Location & Seq
Forward (+)
22908953 .. 22910718
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24800.1

Sequence Viewer

Length: 945 bp
ATGGCGAGGCGGAAGAGGACGCAGTCGCAGTCGGAGTCGGAGGCGCAGGATTTATCTAATAAGACTGTGAAGCGGAAGTTGGAAGAAAAATCGACAACAACTGAAAAGTTAACAGATAATGACGTCACGCATGATGCCATTATACCGTCCCCGCCGCCGAATGTGGAGGTCCGTCGGGCAACCTTTGTTGTAGAAAATGCTTCTCTGAGAAAGGGCATCGTTCGAAAGAGGTGGAAGATTCTGGATTCAGAGGAAGACGCTGATGCCCTGAAACAAAAGAAAGATGAGAATAATTATCGGCCTGGAGTTGTCTACCAGGCTCTTCGTGCGATTTTTGATAGCAAACTTAACAAAGCTGGTATGGTAGGAGCTGTGTATGTGAAAACAGATAATGGAGTGTTATTTGAGATTAAACCCCATGTTCGTATACCAAGAACGTGTAGGCGCTTTTGTGGAGTCATGCGTAAGTTAGCTTCTATTACTAGTTGTCTTATATTCACTCTGCATCATTCAGCCTCTTCAAAATATATCCATGGAACTTACAGATTGCTTTGTGTTTTGACTTTTGCAATTTTTAAAGTGGAGTTGCTAGATAAAAAATGCATACGGACCAAAGATACAAATGAAATTCTTATGCGTGTCATTGCGGAACCTGTAACCCGACATTTACCGGTCAACTCTCGTGTAGTAGGTGAGCATTGCCTACCTCTATTTAGTATTCTGATCTTACTGACGTTGACTAATGCCTGCTGTACTGGGTTTACAGGACTCTCTTATAGTTCAGAAAAAGTGGTTGATATTGATGACTATGTTAATTCTGCCGGTGATGAATTGAATCTTGTTTTTGTGGTGGGTGAACTGGCCCATGGGAAGATCAATGAGTATACAGATGATTTTATATCAGGTAATTTATACTTGAGCTATGTGATAATGTGGCTTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EMG1

Protein Analysis

315

Amino Acids

35.51

Weight (kDa)

8.99

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMG1 PF03587 63 - 157 1e-23 EMG1/NEP1 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 943
AatII GACGTC 1 cut(s) 126
AccI GTMKAC 3 cut(s) 312, 427, 884
AciI CCGC 5 cut(s) 10, 73, 152, 155, 647
AcsI RAATTY 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 1 cut(s) 754
AflIII ACRYGT 1 cut(s) 437
AgeI ACCGGT 1 cut(s) 670
AgsI TTSAA 2 cut(s) 522, 835
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 2 cut(s) 301, 315
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 4 cut(s) 356, 371, 473, 921
AluI AGCT 4 cut(s) 356, 371, 473, 921
AoxI GGCC 2 cut(s) 299, 861
ApoI RAATTY 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 670
AspLEI GCGC 2 cut(s) 46, 447
AspS9I GGNCC 3 cut(s) 169, 609, 862
AsuHPI GGTGA 3 cut(s) 704, 836, 866
AsuII TTCGAA 1 cut(s) 223
AvaII GGWCC 2 cut(s) 169, 609
BarI GAAGNNNNNNTAC 2 cut(s) 457, 489
BauI CACGAG 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 261
BciT130I CCWGG 2 cut(s) 303, 317
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 2 cut(s) 483, 590
BfoI RGCGCY 1 cut(s) 448
BisI GCNGC 1 cut(s) 155
BlsI GCNGC 1 cut(s) 156
Bme1390I CCNGG 2 cut(s) 303, 317
Bme18I GGWCC 2 cut(s) 169, 609
BmgT120I GGNCC 3 cut(s) 169, 609, 862
BmiI GGNNCC 1 cut(s) 651
BmrFI CCNGG 2 cut(s) 303, 317
BmrI ACTGGG 1 cut(s) 765
BmsI GCATC 4 cut(s) 124, 225, 253, 514
BmuI ACTGGG 1 cut(s) 765
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 937
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 532, 865
BsaWI WCCGGW 1 cut(s) 670
Bse118I RCCGGY 2 cut(s) 670, 821
Bse1I ACTGG 2 cut(s) 760, 864
Bse3DI GCAATG 2 cut(s) 642, 697
BseBI CCWGG 2 cut(s) 303, 317
BseDI CCNNGG 2 cut(s) 532, 865
BseMI GCAATG 2 cut(s) 642, 697
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 2 cut(s) 760, 864
BshFI GGCC 2 cut(s) 301, 863
BshTI ACCGGT 1 cut(s) 670
BsiSI CCGG 2 cut(s) 671, 822
BslFI GGGAC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 2 cut(s) 301, 863
Bsp119I TTCGAA 1 cut(s) 223
Bsp143I GATC 2 cut(s) 723, 873
Bsp19I CCATGG 2 cut(s) 532, 865
BspACI CCGC 5 cut(s) 10, 73, 152, 155, 647
BspANI GGCC 2 cut(s) 301, 863
BspCNI CTCAG 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 651
BspQI GCTCTTC 1 cut(s) 327
BspT104I TTCGAA 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 642, 697
BsrFI RCCGGY 2 cut(s) 670, 821
BsrI ACTGG 2 cut(s) 760, 864
BssAI RCCGGY 2 cut(s) 670, 821
BssECI CCNNGG 2 cut(s) 532, 865
BssMI GATC 2 cut(s) 723, 873
BssNAI GTATAC 2 cut(s) 428, 885
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 681
BssT1I CCWWGG 2 cut(s) 532, 865
Bst1107I GTATAC 2 cut(s) 428, 885
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 2 cut(s) 303, 317
Bst4CI ACNGT 2 cut(s) 67, 147
Bst6I CTCTTC 3 cut(s) 8, 327, 523
BstACI GRCGYC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 748
BstDEI CTNAG 1 cut(s) 206
BstDSI CCRYGG 2 cut(s) 532, 865
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 2 cut(s) 46, 447
BstKTI GATC 2 cut(s) 726, 876
BstMBI GATC 2 cut(s) 723, 873
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 2 cut(s) 303, 317
BstSCI CCNGG 2 cut(s) 301, 315
BstV2I GAAGAC 1 cut(s) 261
BstZ17I GTATAC 2 cut(s) 428, 885
BsuRI GGCC 2 cut(s) 301, 863
BtgI CCRYGG 2 cut(s) 532, 865
Cac8I GCNNGC 1 cut(s) 748
CfoI GCGC 2 cut(s) 46, 447
Cfr10I RCCGGY 2 cut(s) 670, 821
Cfr13I GGNCC 3 cut(s) 169, 609, 862
CseI GACGC 2 cut(s) 28, 266
Csp6I GTAC 1 cut(s) 753
CspAI ACCGGT 1 cut(s) 670
CviAII CATG 5 cut(s) 131, 419, 460, 533, 866
CviJI RGCY 9 cut(s) 301, 320, 356, 371, 473, 515, 863, 921, 937
CviKI_1 RGCY 9 cut(s) 301, 320, 356, 371, 473, 515, 863, 921, 937
CviQI GTAC 1 cut(s) 753
DdeI CTNAG 1 cut(s) 206
DpnI GATC 2 cut(s) 725, 875
DpnII GATC 2 cut(s) 723, 873
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 3 cut(s) 8, 327, 523
EarI CTCTTC 3 cut(s) 8, 327, 523
EciI GGCGGA 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 532, 865
Eco47I GGWCC 2 cut(s) 169, 609
EcoRII CCWGG 2 cut(s) 301, 315
EcoT14I CCWWGG 2 cut(s) 532, 865
EcoT22I ATGCAT 1 cut(s) 605
ErhI CCWWGG 2 cut(s) 532, 865
FaeI CATG 5 cut(s) 134, 422, 463, 536, 869
FaqI GGGAC 1 cut(s) 133
FatI CATG 5 cut(s) 130, 418, 459, 532, 865
FauI CCCGC 1 cut(s) 159
FblI GTMKAC 3 cut(s) 312, 427, 884
Fnu4HI GCNGC 1 cut(s) 155
Fsp4HI GCNGC 1 cut(s) 155
FspBI CTAG 2 cut(s) 483, 590
GlaI GCGC 2 cut(s) 45, 446
GluI GCNGC 1 cut(s) 155
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 2 cut(s) 301, 863
HapII CCGG 2 cut(s) 671, 822
HgaI GACGC 2 cut(s) 28, 266
HhaI GCGC 2 cut(s) 46, 447
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 5 cut(s) 134, 422, 463, 536, 869
Hin6I GCGC 2 cut(s) 44, 445
HinP1I GCGC 2 cut(s) 44, 445
HincII GTYRAC 3 cut(s) 111, 676, 738
HindII GTYRAC 3 cut(s) 111, 676, 738
HinfI GANTC 6 cut(s) 35, 238, 245, 456, 768, 835
HpaI GTTAAC 1 cut(s) 111
HpaII CCGG 2 cut(s) 671, 822
HphI GGTGA 3 cut(s) 704, 836, 866
Hpy166II GTNNAC 8 cut(s) 111, 313, 428, 676, 738, 762, 857, 885
Hpy188I TCNGA 6 cut(s) 34, 40, 207, 250, 723, 784
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 8 cut(s) 111, 313, 428, 676, 738, 762, 857, 885
Hpy99I CGWCG 1 cut(s) 177
HpyCH4III ACNGT 2 cut(s) 67, 147
HpyCH4IV ACGT 3 cut(s) 123, 437, 734
HpyCH4V TGCA 3 cut(s) 505, 569, 603
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 1 cut(s) 206
HpySE526I ACGT 3 cut(s) 123, 437, 734
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 5 cut(s) 134, 422, 463, 536, 869
HspAI GCGC 2 cut(s) 44, 445
KspAI GTTAAC 1 cut(s) 111
Kzo9I GATC 2 cut(s) 723, 873
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 1 cut(s) 368
LweI GCATC 4 cut(s) 124, 225, 253, 514
MaeI CTAG 2 cut(s) 483, 590
MaeII ACGT 3 cut(s) 123, 437, 734
MaeIII GTNAC 2 cut(s) 124, 655
MalI GATC 2 cut(s) 725, 875
MboI GATC 2 cut(s) 723, 873
MboII GAAGA 7 cut(s) 25, 95, 247, 266, 314, 510, 883
MluCI AATT 6 cut(s) 292, 570, 627, 814, 830, 907
MlyI GAGTC 3 cut(s) 44, 465, 762
MmeI TCCRAC 3 cut(s) 12, 18, 60
MnlI CCTC 7 cut(s) 9, 34, 160, 222, 244, 526, 717
Mph1103I ATGCAT 1 cut(s) 605
MseI TTAA 5 cut(s) 110, 348, 411, 576, 813
MspI CCGG 2 cut(s) 671, 822
MspR9I CCNGG 2 cut(s) 303, 317
MvaI CCWGG 2 cut(s) 303, 317
MwoI GCNNNNNNNGC 1 cut(s) 326
NcoI CCATGG 2 cut(s) 532, 865
NdeII GATC 2 cut(s) 723, 873
NlaIII CATG 5 cut(s) 134, 422, 463, 536, 869
NlaIV GGNNCC 1 cut(s) 651
NmuCI GTSAC 1 cut(s) 124
NsiI ATGCAT 1 cut(s) 605
NspV TTCGAA 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 327
PfeI GAWTC 3 cut(s) 238, 245, 835
PflFI GACNNNGTC 1 cut(s) 22
PinAI ACCGGT 1 cut(s) 670
PkrI GCNGC 1 cut(s) 156
PleI GAGTC 3 cut(s) 43, 464, 762
PpsI GAGTC 3 cut(s) 43, 464, 762
PsiI TTATAA 1 cut(s) 943
Psp6I CCWGG 2 cut(s) 301, 315
PspGI CCWGG 2 cut(s) 301, 315
PspN4I GGNNCC 1 cut(s) 651
PspPI GGNCC 3 cut(s) 169, 609, 862
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 1 cut(s) 754
RsaNI GTAC 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 5 cut(s) 110, 348, 411, 576, 813
SatI GCNGC 1 cut(s) 155
Sau3AI GATC 2 cut(s) 723, 873
Sau96I GGNCC 3 cut(s) 169, 609, 862
SchI GAGTC 3 cut(s) 44, 465, 762
ScrFI CCNGG 2 cut(s) 303, 317
SfaNI GCATC 4 cut(s) 124, 225, 253, 514
SfuI TTCGAA 1 cut(s) 223
SinI GGWCC 2 cut(s) 169, 609
SmlI CTYRAG 1 cut(s) 916
SmoI CTYRAG 1 cut(s) 916
SpeI ACTAGT 1 cut(s) 482
Sse9I AATT 6 cut(s) 292, 570, 627, 814, 830, 907
SsiI CCGC 5 cut(s) 10, 73, 152, 155, 647
SspMI CTAG 2 cut(s) 483, 590
StyD4I CCNGG 2 cut(s) 301, 315
StyI CCWWGG 2 cut(s) 532, 865
TaaI ACNGT 2 cut(s) 67, 147
TaiI ACGT 3 cut(s) 126, 440, 737
TaqI TCGA 2 cut(s) 92, 223
TasI AATT 6 cut(s) 292, 570, 627, 814, 830, 907
TatI WGTACW 1 cut(s) 752
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 3 cut(s) 238, 245, 835
Tru1I TTAA 5 cut(s) 110, 348, 411, 576, 813
Tru9I TTAA 5 cut(s) 110, 348, 411, 576, 813
TseFI GTSAC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 2 cut(s) 639, 843
TspGWI ACGGA 2 cut(s) 161, 622
Tth111I GACNNNGTC 1 cut(s) 22
VpaK11BI GGWCC 2 cut(s) 169, 609
XapI RAATTY 1 cut(s) 627
XmiI GTMKAC 3 cut(s) 312, 427, 884
XspI CTAG 2 cut(s) 483, 590
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.