MD03G1012500.v1.1

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001307
Physical Location & Seq
Forward (+)
947645 .. 949860
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1012500.v1.1.491

Sequence Viewer

Length: 1734 bp
ATGAGCATCACAAGCCCGCCTTCCATGAAGCACTCATCACCTACGAAAGCAGAGCCGAAGGCCCCTTTGCTTTCAATCTTGTTCTCCAAAAGAAAATCCATGGCCTTCACTTATGTATTCATGTTTGCCTTCATTGCCTGCACTTTCTTTTTGGTTTTCAGCCCGTCTCGCCAAAACGGCCCTCTTCCTCTCCGCTTCAAGAACCTGCTTCATGCCTCCTTCTACTCTTCTTCTCAGAACCCTAATGCACATTTGGTGCTTTCTCGGAGAGACCCTTTTTCCGGGAAACCTAATTTTTCGCCACAGTTTTATGAGGTTGCAAGTTTGAAGGGTCATAAGGTGGATAAGCATGAGATTGGTCTCTCTCAATTTCCGGAGAAAAACGAAAGCTTGAAGGGAGATAATAACAATGCTCAATCATCCAACCCCATTCTTTTATTGTCCAACAACACGGAAATTGAATTTTCCGGGAAAATGGAGGGTTCCAGGAGAAGAAACGAGACCAAGAAGCATGCACATTCGAATTCTCCTTCTTCGAAAGATTCAAATGGAAAACGTGGCTCGAAGAAGAAAACTGATAGTAAAATGATGTCATCTAAGAAGCAAGGGAAACAAAGTGAGTTGAAGTCCAAGTTGACGAATGGCTGTGACATATTTGATGGAAGATGGGTGAGAGATGATTCGTACCCACTTTATGCTCCGGGGTCTTGTCCTTGGATCGATGAGCCTTTCGATTGTTTTCTGAATGGAAGGCCAGACAATGGATACGAGAGGTATAGATGGCAACCAAAACGTTGCAATATCCCAAGGTTGAATGGTAAGAAGATGTTGAGATTGTTGACGGGGAAGCGTCTGGTTTTTGTTGGCGATTCTCTCAACAGGAATATGTGGGAGTCTTTGCTATGTGTTCTTAGAAACTCAGTGGATGATAAGAACAAAGTCTATGAAGTTTCTGGTAGGCAAGAATTTCGCACAGAGGGCTCTTACTCCTTTGTTTTCGAGGAGTACAACTGTTCCGTGGAGTTCTTTCAATCACGGTTTCTAGTTCAAGAATGGGAGATGCCGGAACCAAGTGGATCAAAGAAGGAAACGCTTCGCATTGATTTGATTGAGAGATCCTCTGACAACTACAAAAATGCAGATGTTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCCAGCGGGAAGGGTTACTATCAAGAGGGTAGCCATATTTATGGTGAACTGAATGTTGACGATGCATTTCGCAAGGCTTTAACGACATGGGCTCAATGGATAGACACCAATGTAAATCCTAAGAAAACTGCTGTTTTCTTCCGGGGCTATTCACCTTCTCACTTTAGAGGCGGAGCATGGAATTCTGGAGGGCATTGCCATGGCGAAACAAAGCCAACTACCAGCTTGGAATCCACGGAATATGGAGGAGAGTATCCAACAAACATGAAAATCTTTGACTCGGTACTCAAGGGGATGAAGACGCCAGTCAACTATCTGAACATTACAACAATGACTGATTTTCGGAAGGATGCTCATCCATCGATTTACAGAAAGCCGAATTTAACTGAGGAGGAGCGAAAACGGCCTATGATCCAGGATTGCAGTCACTGGTGCCTCCCTGGCGTTCCTGATACATGGAACGAGCTTATATATGCTCAACTCCTCAAACATAACCAGAAACAGCATAGACAGAAGAAACAGCAAAATCAGAGGACAACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

578

Amino Acids

66.36

Weight (kDa)

9.45

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 215 - 267 5.1e-22 PMR5 N terminal Domain
PC-Esterase PF13839 268 - 555 8.7e-102 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 213
AccB1I GGYRCC 1 cut(s) 1620
AccB7I CCANNNNNTGG 1 cut(s) 761
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 4 cut(s) 17, 193, 1194, 1359
AclI AACGTT 1 cut(s) 793
AclWI GGATC 4 cut(s) 725, 1084, 1110, 1594
AcsI RAATTY 5 cut(s) 461, 523, 965, 1369, 1567
AcyI GRCGYC 1 cut(s) 1490
AfaI GTAC 3 cut(s) 686, 1007, 1473
AfiI CCNNNNNNNGG 2 cut(s) 281, 761
AjnI CCWGG 3 cut(s) 485, 1602, 1627
AluBI AGCT 3 cut(s) 390, 1413, 1654
AluI AGCT 3 cut(s) 390, 1413, 1654
Alw26I GTCTC 4 cut(s) 171, 264, 365, 494
AlwI GGATC 4 cut(s) 725, 1084, 1110, 1594
AlwNI CAGNNNCTG 1 cut(s) 1617
Aor13HI TCCGGA 1 cut(s) 373
AoxI GGCC 5 cut(s) 60, 102, 178, 752, 1592
ApoI RAATTY 5 cut(s) 461, 523, 965, 1369, 1567
Asp700I GAANNNNTTC 1 cut(s) 1092
AspS9I GGNCC 2 cut(s) 61, 179
AsuC2I CCSGG 4 cut(s) 283, 469, 702, 1331
AsuHPI GGTGA 4 cut(s) 30, 682, 1244, 1332
AsuII TTCGAA 2 cut(s) 521, 536
BanI GGYRCC 1 cut(s) 1620
BanII GRGCYC 2 cut(s) 983, 1282
BbsI GAAGAC 1 cut(s) 1493
BccI CCATC 4 cut(s) 653, 660, 774, 1555
BceAI ACGGC 2 cut(s) 193, 1607
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 3 cut(s) 487, 1604, 1629
BciVI GTATCC 2 cut(s) 758, 1452
BcnI CCSGG 4 cut(s) 283, 469, 702, 1331
BcoDI GTCTC 4 cut(s) 171, 264, 365, 494
BfaI CTAG 1 cut(s) 1043
BfuAI ACCTGC 1 cut(s) 213
BfuI GTATCC 2 cut(s) 758, 1452
BglI GCCNNNNNGGC 2 cut(s) 177, 1629
Bme1390I CCNGG 7 cut(s) 283, 469, 487, 702, 1331, 1604, 1629
BmgT120I GGNCC 2 cut(s) 61, 179
BmiI GGNNCC 4 cut(s) 63, 484, 1068, 1622
BmrFI CCNGG 7 cut(s) 283, 469, 487, 702, 1331, 1604, 1629
BmsI GCATC 4 cut(s) 15, 1050, 1240, 1528
BoxI GACNNNNGTC 1 cut(s) 1493
BpiI GAAGAC 1 cut(s) 1493
BplI GAGNNNNNCTC 2 cut(s) 1103, 1135
BpmI CTGGAG 1 cut(s) 1395
Bpu14I TTCGAA 2 cut(s) 521, 536
BpuEI CTTGAG 1 cut(s) 1460
BpuMI CCSGG 4 cut(s) 283, 469, 702, 1331
Bsa29I ATCGAT 2 cut(s) 720, 1550
BsaBI GATNNNNATC 1 cut(s) 1542
BsaHI GRCGYC 1 cut(s) 1490
BsaI GGTCTC 3 cut(s) 264, 365, 494
BsaJI CCNNGG 9 cut(s) 99, 701, 713, 806, 1017, 1330, 1387, 1422, 1627
BsaWI WCCGGW 1 cut(s) 373
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 761
Bse1I ACTGG 3 cut(s) 1172, 1493, 1622
Bse3DI GCAATG 2 cut(s) 132, 1381
Bse8I GATNNNNATC 1 cut(s) 1542
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 3 cut(s) 487, 1604, 1629
BseCI ATCGAT 2 cut(s) 720, 1550
BseDI CCNNGG 9 cut(s) 99, 701, 713, 806, 1017, 1330, 1387, 1422, 1627
BseGI GGATG 5 cut(s) 419, 931, 1488, 1543, 1543
BseJI GATNNNNATC 1 cut(s) 1542
BseLI CCNNNNNNNGG 2 cut(s) 281, 761
BseMI GCAATG 2 cut(s) 132, 1381
BseMII CTCAG 3 cut(s) 248, 933, 1566
BseNI ACTGG 3 cut(s) 1172, 1493, 1622
BseRI GAGGAG 5 cut(s) 1016, 1449, 1592, 1595, 1661
BsgI GTGCAG 1 cut(s) 124
BshFI GGCC 5 cut(s) 62, 104, 180, 754, 1594
BshNI GGYRCC 1 cut(s) 1620
BshVI ATCGAT 2 cut(s) 720, 1550
BsiSI CCGG 6 cut(s) 282, 374, 468, 701, 1064, 1330
BslI CCNNNNNNNGG 2 cut(s) 281, 761
BsmAI GTCTC 4 cut(s) 171, 264, 365, 494
BsmBI CGTCTC 1 cut(s) 171
BsnI GGCC 5 cut(s) 62, 104, 180, 754, 1594
Bso31I GGTCTC 3 cut(s) 264, 365, 494
Bsp119I TTCGAA 2 cut(s) 521, 536
Bsp1286I GDGCHC 2 cut(s) 983, 1282
Bsp13I TCCGGA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 717, 1076, 1115, 1599
Bsp19I CCATGG 2 cut(s) 99, 1387
BspACI CCGC 4 cut(s) 17, 193, 1194, 1359
BspANI GGCC 5 cut(s) 62, 104, 180, 754, 1594
BspCNI CTCAG 3 cut(s) 247, 932, 1567
BspDI ATCGAT 2 cut(s) 720, 1550
BspEI TCCGGA 1 cut(s) 373
BspHI TCATGA 1 cut(s) 1177
BspLI GGNNCC 4 cut(s) 63, 484, 1068, 1622
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 4 cut(s) 725, 1084, 1110, 1594
BspT104I TTCGAA 2 cut(s) 521, 536
BspT107I GGYRCC 1 cut(s) 1620
BspTNI GGTCTC 3 cut(s) 264, 365, 494
BsrDI GCAATG 2 cut(s) 132, 1381
BsrI ACTGG 3 cut(s) 1172, 1493, 1622
BssECI CCNNGG 9 cut(s) 99, 701, 713, 806, 1017, 1330, 1387, 1422, 1627
BssMI GATC 4 cut(s) 717, 1076, 1115, 1599
BssNI GRCGYC 1 cut(s) 1490
BssT1I CCWWGG 4 cut(s) 99, 713, 806, 1387
Bst2UI CCWGG 3 cut(s) 487, 1604, 1629
Bst4CI ACNGT 3 cut(s) 306, 1013, 1038
Bst6I CTCTTC 2 cut(s) 189, 232
BstACI GRCGYC 1 cut(s) 1490
BstBI TTCGAA 2 cut(s) 521, 536
BstC8I GCNNGC 3 cut(s) 17, 139, 513
BstDEI CTNAG 6 cut(s) 234, 597, 911, 919, 1308, 1575
BstDSI CCRYGG 4 cut(s) 99, 1017, 1387, 1422
BstF5I GGATG 5 cut(s) 419, 931, 1488, 1543, 1543
BstKTI GATC 4 cut(s) 720, 1079, 1118, 1602
BstMAI GTCTC 4 cut(s) 171, 264, 365, 494
BstMBI GATC 4 cut(s) 717, 1076, 1115, 1599
BstMWI GCNNNNNNNGC 7 cut(s) 12, 134, 168, 177, 978, 1591, 1629
BstNI CCWGG 3 cut(s) 487, 1604, 1629
BstNSI RCATGY 1 cut(s) 515
BstPAI GACNNNNGTC 1 cut(s) 1493
BstSCI CCNGG 7 cut(s) 281, 467, 485, 700, 1329, 1602, 1627
BstV2I GAAGAC 1 cut(s) 1493
BstX2I RGATCY 1 cut(s) 1115
BstXI CCANNNNNNTGG 1 cut(s) 1229
BstYI RGATCY 1 cut(s) 1115
Bsu15I ATCGAT 2 cut(s) 720, 1550
BsuI GTATCC 2 cut(s) 758, 1452
BsuRI GGCC 5 cut(s) 62, 104, 180, 754, 1594
BsuTUI ATCGAT 2 cut(s) 720, 1550
BtgI CCRYGG 4 cut(s) 99, 1017, 1387, 1422
BtsCI GGATG 5 cut(s) 419, 931, 1488, 1543, 1543
BtsIMutI CAGTG 3 cut(s) 927, 1165, 1615
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 3 cut(s) 17, 139, 513
CaiI CAGNNNCTG 1 cut(s) 1617
CciI TCATGA 1 cut(s) 1177
Cfr13I GGNCC 2 cut(s) 61, 179
ClaI ATCGAT 2 cut(s) 720, 1550
CseI GACGC 2 cut(s) 839, 1498
Csp6I GTAC 3 cut(s) 685, 1006, 1472
CviQI GTAC 3 cut(s) 685, 1006, 1472
DdeI CTNAG 6 cut(s) 234, 597, 911, 919, 1308, 1575
DpnI GATC 4 cut(s) 719, 1078, 1117, 1601
DpnII GATC 4 cut(s) 717, 1076, 1115, 1599
Eam1104I CTCTTC 2 cut(s) 189, 232
EarI CTCTTC 2 cut(s) 189, 232
EciI GGCGGA 1 cut(s) 1374
Eco130I CCWWGG 4 cut(s) 99, 713, 806, 1387
Eco24I GRGCYC 2 cut(s) 983, 1282
Eco31I GGTCTC 3 cut(s) 264, 365, 494
EcoO109I RGGNCCY 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 523, 1369
EcoRII CCWGG 3 cut(s) 485, 1602, 1627
EcoT14I CCWWGG 4 cut(s) 99, 713, 806, 1387
EcoT22I ATGCAT 1 cut(s) 1255
EcoT38I GRGCYC 2 cut(s) 983, 1282
ErhI CCWWGG 4 cut(s) 99, 713, 806, 1387
Esp3I CGTCTC 1 cut(s) 171
FalI AAGNNNNNCTT 1 cut(s) 36
FauI CCCGC 2 cut(s) 24, 1187
FokI GGATG 5 cut(s) 406, 938, 1495, 1530, 1550
FriOI GRGCYC 2 cut(s) 983, 1282
FspBI CTAG 1 cut(s) 1043
GsuI CTGGAG 1 cut(s) 1395
HaeIII GGCC 5 cut(s) 62, 104, 180, 754, 1594
HapII CCGG 6 cut(s) 282, 374, 468, 701, 1064, 1330
HgaI GACGC 2 cut(s) 839, 1498
Hin1I GRCGYC 1 cut(s) 1490
HincII GTYRAC 4 cut(s) 636, 840, 1246, 1498
HindII GTYRAC 4 cut(s) 636, 840, 1246, 1498
HindIII AAGCTT 1 cut(s) 388
HinfI GANTC 7 cut(s) 542, 680, 869, 893, 1174, 1418, 1466
HpaII CCGG 6 cut(s) 282, 374, 468, 701, 1064, 1330
HphI GGTGA 4 cut(s) 30, 682, 1244, 1332
Hpy166II GTNNAC 6 cut(s) 636, 840, 1173, 1235, 1246, 1498
Hpy188I TCNGA 7 cut(s) 237, 267, 744, 1123, 1506, 1533, 1719
Hpy188III TCNNGA 7 cut(s) 199, 374, 1049, 1178, 1211, 1374, 1637
Hpy8I GTNNAC 6 cut(s) 636, 840, 1173, 1235, 1246, 1498
HpyCH4III ACNGT 3 cut(s) 306, 1013, 1038
HpyCH4IV ACGT 2 cut(s) 556, 793
HpyCH4V TGCA 8 cut(s) 141, 248, 320, 515, 798, 1139, 1253, 1611
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 134, 168, 177, 978, 1591, 1629
HpyF3I CTNAG 6 cut(s) 234, 597, 911, 919, 1308, 1575
HpySE526I ACGT 2 cut(s) 556, 793
Hsp92I GRCGYC 1 cut(s) 1490
Kpn2I TCCGGA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 717, 1076, 1115, 1599
LmnI GCTCC 3 cut(s) 703, 1361, 1582
LweI GCATC 4 cut(s) 15, 1050, 1240, 1528
MaeI CTAG 1 cut(s) 1043
MaeII ACGT 2 cut(s) 556, 793
MaeIII GTNAC 4 cut(s) 647, 1163, 1202, 1613
MalI GATC 4 cut(s) 719, 1078, 1117, 1601
MboI GATC 4 cut(s) 717, 1076, 1115, 1599
MflI RGATCY 1 cut(s) 1115
MhlI GDGCHC 2 cut(s) 983, 1282
MluCI AATT 8 cut(s) 292, 368, 456, 461, 523, 965, 1369, 1567
MlyI GAGTC 3 cut(s) 902, 1168, 1460
MmeI TCCRAC 3 cut(s) 447, 468, 1469
Mph1103I ATGCAT 1 cut(s) 1255
MroI TCCGGA 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 1092
MseI TTAA 3 cut(s) 1268, 1571, 1732
MslI CAYNNNNRTG 2 cut(s) 1227, 1386
MspA1I CMGCKG 1 cut(s) 1194
MspI CCGG 6 cut(s) 282, 374, 468, 701, 1064, 1330
MspR9I CCNGG 7 cut(s) 283, 469, 487, 702, 1331, 1604, 1629
MvaI CCWGG 3 cut(s) 487, 1604, 1629
MwoI GCNNNNNNNGC 7 cut(s) 12, 134, 168, 177, 978, 1591, 1629
NciI CCSGG 4 cut(s) 283, 469, 702, 1331
NcoI CCATGG 2 cut(s) 99, 1387
NdeII GATC 4 cut(s) 717, 1076, 1115, 1599
NlaIV GGNNCC 4 cut(s) 63, 484, 1068, 1622
NmuCI GTSAC 3 cut(s) 647, 1163, 1613
NsiI ATGCAT 1 cut(s) 1255
NspI RCATGY 1 cut(s) 515
NspV TTCGAA 2 cut(s) 521, 536
PaeI GCATGC 1 cut(s) 515
PagI TCATGA 1 cut(s) 1177
PdmI GAANNNNTTC 1 cut(s) 1092
PfeI GAWTC 4 cut(s) 542, 680, 869, 1418
PflMI CCANNNNNTGG 1 cut(s) 761
PfoI TCCNGGA 4 cut(s) 281, 467, 485, 1602
PleI GAGTC 3 cut(s) 901, 1168, 1460
PpsI GAGTC 3 cut(s) 901, 1168, 1460
PshAI GACNNNNGTC 1 cut(s) 1493
Psp1406I AACGTT 1 cut(s) 793
Psp6I CCWGG 3 cut(s) 485, 1602, 1627
PspGI CCWGG 3 cut(s) 485, 1602, 1627
PspN4I GGNNCC 4 cut(s) 63, 484, 1068, 1622
PspPI GGNCC 2 cut(s) 61, 179
PstNI CAGNNNCTG 1 cut(s) 1617
PsuI RGATCY 1 cut(s) 1115
RsaI GTAC 3 cut(s) 686, 1007, 1473
RsaNI GTAC 3 cut(s) 685, 1006, 1472
RseI CAYNNNNRTG 2 cut(s) 1227, 1386
SaqAI TTAA 3 cut(s) 1268, 1571, 1732
Sau3AI GATC 4 cut(s) 717, 1076, 1115, 1599
Sau96I GGNCC 2 cut(s) 61, 179
SchI GAGTC 3 cut(s) 902, 1168, 1460
ScrFI CCNGG 7 cut(s) 283, 469, 487, 702, 1331, 1604, 1629
SduI GDGCHC 2 cut(s) 983, 1282
SfaNI GCATC 4 cut(s) 15, 1050, 1240, 1528
SfuI TTCGAA 2 cut(s) 521, 536
SmiMI CAYNNNNRTG 2 cut(s) 1227, 1386
SmlI CTYRAG 1 cut(s) 1475
SmoI CTYRAG 1 cut(s) 1475
SphI GCATGC 1 cut(s) 515
Sse9I AATT 8 cut(s) 292, 368, 456, 461, 523, 965, 1369, 1567
SsiI CCGC 4 cut(s) 17, 193, 1194, 1359
SspMI CTAG 1 cut(s) 1043
StyD4I CCNGG 7 cut(s) 281, 467, 485, 700, 1329, 1602, 1627
StyI CCWWGG 4 cut(s) 99, 713, 806, 1387
TaaI ACNGT 3 cut(s) 306, 1013, 1038
TaiI ACGT 2 cut(s) 559, 796
TaqI TCGA 7 cut(s) 521, 536, 563, 720, 732, 999, 1550
TasI AATT 8 cut(s) 292, 368, 456, 461, 523, 965, 1369, 1567
TatI WGTACW 1 cut(s) 1005
TfiI GAWTC 4 cut(s) 542, 680, 869, 1418
Tru1I TTAA 3 cut(s) 1268, 1571, 1732
Tru9I TTAA 3 cut(s) 1268, 1571, 1732
TscAI CASTG 3 cut(s) 927, 1172, 1622
TseFI GTSAC 3 cut(s) 647, 1163, 1613
Tsp45I GTSAC 3 cut(s) 647, 1163, 1613
TspDTI ATGAA 7 cut(s) 41, 109, 121, 200, 960, 1469, 1499
TspGWI ACGGA 3 cut(s) 467, 1006, 1439
TspRI CASTG 3 cut(s) 927, 1172, 1622
Van91I CCANNNNNTGG 1 cut(s) 761
XapI RAATTY 5 cut(s) 461, 523, 965, 1369, 1567
XceI RCATGY 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 1 cut(s) 1043
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.