MD03G1013100.v1.1

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001307
Physical Location & Seq
Forward (+)
1035166 .. 1037818
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1013100.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGCGGATTACTCAACCCAGACAACCCCAAAACCCACAAAGCACCACCACTCCCCCACAATCCCAAGACCAGAGGCCTTCTCCAAGCCCTCTCTTTCTTCCCCAATCTTCTTCCCCTCCAGAAGAACCGCCGCCTTTGCCTATGGCTTCTCGTTCGTCTGCATCGCTTGCACTTTCTTCCTGGTTTTCAACCCATCTGGCTACTCTTTCCCTTTCGGCTTCAAGAACATCTTCAAAACCCCTTTTGAACTTTCCCGGGAAACCCCATTTTCCTGGAAAGCTGAATATTTCCCGCGTTTGGATGAGAGTGTAGGTTTGCAGTCTGATATGGCTAGTGCCCAACTGCCGAACCCAATTCTTTTCTTGCCCCAAGAATCCAATCAGACTTTGTCCCAGAACACTACGATTCAAGTAACTGGGAAAATGGGATTTTCCGGGAAACTGGAGGTGGCATTGACCTCGAAAGAGAGTGATGATGCTAGTGAGGCATTGTCTGAATCTGAGAAGCAGAGCAATCAAACTCAGCCCGGTTATGAATTTTCGAGGGATGATTGTGACCTGTTTGATGGAATGTGGGTGAGAGATGATTCGTATCCGCTTTATGCTCCGGGTTCTTGTCCTCTTATTGATGAATCCTTCAATTGTTTTCTTAATAAGAGGCCGGATAATCGCTACGAAAAGTATAGATGGCAACCCAAACATTGCAATCTTCCAAGCTTGTTGGATGGTAAGAACATGTTGGGACTATTGAGAGGAAAGCGACTGGTTTTTGTTGGGGATTCTCTTAATAGGAATATGTGGGAGTCTTTGCTTTGTCTTCTTCGAAATTCAGTGGATGATAAAAGTGGAGTTGTTGAAGCGTTGGGTAGGAGCGAATTTCGTTCAGAGGGATCTTACTCTTTCGTATTCAAGGAGTACAATTGCTCAGTGGAATTCTTTCGATCGCCATTTCTAGTTCAAGAATGGGAGATGGCAGATGCAAATGGATCAATAAAGGAAACGCTTCGCCTTGATTTGATTGAGAGATCCTCCGATAAGTACAAAACTGCAGATGTTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCCAGAGGGAAGGGTTACTATCAAGAGGGTAGCTACATCTACGATGTATTGAACGTTAAAGAGGCATTTCGGAAAGCTTTAACAACATGGGCAAGATGGGTGGATACCAACATAAATCCTGAGAAAACTATTGTTTTCTTCCGGGGCTTTTCACCTAATCACTTTAGAGGTGGAAGATGGAATTCGGGAGGGCAATGCCATGATCTAATAGAGCCACTTGCAAAGGAGACAAATAGAGGAAAATATGCATCAAAATTGAGAATATTTGACTCAGTAATCACTGGAATGAAGACGCCAATATTCTATCTAAACATTACGAGAATGACTGATTTCAGGAGGGATGCTCATCCATCGATTTACAGAAAGCAGAATTTTACAGAGGAGGAGCGGAAATCGCCTTTGAGACACCAGGATTGCAGTCACTGGTGCCTCCCTGGTGTTCCTGATACATGGAATGAGCTTCTATATGCTCAGCTCGTTCGACATAACCAGAAGCAACAACAACAGCAGCGTCAAGACCCACAGCAACAGAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

546

Amino Acids

63.01

Weight (kDa)

8.34

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 184 - 236 3.2e-22 PMR5 N terminal Domain
PC-Esterase PF13839 241 - 523 7e-98 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1524
AccBSI CCGCTC 1 cut(s) 1486
AccII CGCG 1 cut(s) 295
AciI CCGC 6 cut(s) 5, 129, 132, 293, 596, 1486
AclI AACGTT 1 cut(s) 1152
AclWI GGATC 3 cut(s) 898, 994, 1020
AcsI RAATTY 6 cut(s) 536, 826, 875, 932, 1279, 1468
AcyI GRCGYC 1 cut(s) 1391
AfaI GTAC 2 cut(s) 917, 1040
AfiI CCNNNNNNNGG 1 cut(s) 298
AflIII ACRYGT 1 cut(s) 735
AjnI CCWGG 4 cut(s) 180, 272, 1506, 1531
AluBI AGCT 6 cut(s) 281, 717, 1131, 1175, 1558, 1573
AluI AGCT 6 cut(s) 281, 717, 1131, 1175, 1558, 1573
Alw26I GTCTC 2 cut(s) 1319, 1495
AlwI GGATC 3 cut(s) 898, 994, 1020
AlwNI CAGNNNCTG 1 cut(s) 1521
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 2 cut(s) 75, 659
ApeKI GCWGC 1 cut(s) 1606
ApoI RAATTY 6 cut(s) 536, 826, 875, 932, 1279, 1468
Asp700I GAANNNNTTC 3 cut(s) 230, 936, 1002
AsuC2I CCSGG 6 cut(s) 256, 257, 436, 528, 609, 1241
AsuHPI GGTGA 2 cut(s) 589, 1242
AsuII TTCGAA 1 cut(s) 823
AvaI CYCGRG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 1524
BbsI GAAGAC 2 cut(s) 809, 1394
BbvI GCAGC 1 cut(s) 1618
BccI CCATC 8 cut(s) 202, 560, 681, 719, 964, 1188, 1269, 1456
BcgI CGANNNNNNTGC 2 cut(s) 441, 475
BciT130I CCWGG 4 cut(s) 182, 274, 1508, 1533
BciVI GTATCC 2 cut(s) 603, 1195
BcnI CCSGG 6 cut(s) 256, 257, 436, 528, 609, 1241
BcoDI GTCTC 2 cut(s) 1319, 1495
BfaI CTAG 4 cut(s) 333, 480, 953, 1636
BfmI CTRYAG 1 cut(s) 1047
BfuI GTATCC 2 cut(s) 603, 1195
BisI GCNGC 2 cut(s) 132, 1607
BlpI GCTNAGC 1 cut(s) 1569
BlsI GCNGC 2 cut(s) 133, 1608
BmeT110I CYCGRG 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 1526
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 5 cut(s) 171, 466, 967, 1355, 1429
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 2 cut(s) 809, 1394
BplI GAGNNNNNCTC 6 cut(s) 65, 97, 1013, 1045, 1426, 1458
BpmI CTGGAG 2 cut(s) 103, 464
Bpu1102I GCTNAGC 1 cut(s) 1569
Bpu14I TTCGAA 1 cut(s) 823
BpuMI CCSGG 6 cut(s) 256, 257, 436, 528, 609, 1241
Bsa29I ATCGAT 1 cut(s) 1451
BsaBI GATNNNNATC 2 cut(s) 591, 1443
BsaHI GRCGYC 1 cut(s) 1391
BsaJI CCNNGG 3 cut(s) 255, 1240, 1531
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 6 cut(s) 421, 447, 768, 1082, 1384, 1526
Bse3DI GCAATG 2 cut(s) 700, 1298
Bse8I GATNNNNATC 2 cut(s) 591, 1443
BseBI CCWGG 4 cut(s) 182, 274, 1508, 1533
BseCI ATCGAT 1 cut(s) 1451
BseDI CCNNGG 3 cut(s) 255, 1240, 1531
BseGI GGATG 6 cut(s) 307, 553, 730, 841, 1444, 1444
BseJI GATNNNNATC 2 cut(s) 591, 1443
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 2 cut(s) 700, 1298
BseMII CTCAG 6 cut(s) 492, 536, 939, 1209, 1383, 1583
BseNI ACTGG 6 cut(s) 421, 447, 768, 1082, 1384, 1526
BseRI GAGGAG 2 cut(s) 1493, 1496
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 1 cut(s) 1618
Bsh1236I CGCG 1 cut(s) 295
Bsh1285I CGRYCG 1 cut(s) 944
BshFI GGCC 2 cut(s) 77, 661
BshNI GGYRCC 1 cut(s) 1524
BshVI ATCGAT 1 cut(s) 1451
BsiEI CGRYCG 1 cut(s) 944
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 6 cut(s) 256, 435, 528, 608, 662, 1240
BslFI GGGAC 2 cut(s) 376, 756
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 2 cut(s) 1319, 1495
BsmFI GGGAC 2 cut(s) 376, 756
BsnI GGCC 2 cut(s) 77, 661
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 823
Bsp1286I GDGCHC 1 cut(s) 340
Bsp143I GATC 5 cut(s) 890, 941, 986, 1025, 1300
Bsp1720I GCTNAGC 1 cut(s) 1569
BspACI CCGC 6 cut(s) 5, 129, 132, 293, 596, 1486
BspANI GGCC 2 cut(s) 77, 661
BspCNI CTCAG 6 cut(s) 493, 535, 938, 1210, 1382, 1582
BspDI ATCGAT 1 cut(s) 1451
BspFNI CGCG 1 cut(s) 295
BspHI TCATGA 1 cut(s) 1087
BspLI GGNNCC 1 cut(s) 1526
BspMAI CTGCAG 1 cut(s) 1051
BspPI GGATC 3 cut(s) 898, 994, 1020
BspT104I TTCGAA 1 cut(s) 823
BspT107I GGYRCC 1 cut(s) 1524
BsrBI CCGCTC 1 cut(s) 1486
BsrDI GCAATG 2 cut(s) 700, 1298
BsrI ACTGG 6 cut(s) 421, 447, 768, 1082, 1384, 1526
BssECI CCNNGG 3 cut(s) 255, 1240, 1531
BssMI GATC 5 cut(s) 890, 941, 986, 1025, 1300
BssNI GRCGYC 1 cut(s) 1391
Bst2UI CCWGG 4 cut(s) 182, 274, 1508, 1533
BstACI GRCGYC 1 cut(s) 1391
BstAPI GCANNNNNTGC 1 cut(s) 168
BstBI TTCGAA 1 cut(s) 823
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 6 cut(s) 501, 522, 925, 1218, 1369, 1569
BstF5I GGATG 6 cut(s) 307, 553, 730, 841, 1444, 1444
BstFNI CGCG 1 cut(s) 295
BstKTI GATC 5 cut(s) 893, 944, 989, 1028, 1303
BstMAI GTCTC 2 cut(s) 1319, 1495
BstMBI GATC 5 cut(s) 890, 941, 986, 1025, 1300
BstMCI CGRYCG 1 cut(s) 944
BstMWI GCNNNNNNNGC 4 cut(s) 137, 168, 485, 1492
BstNI CCWGG 4 cut(s) 182, 274, 1508, 1533
BstNSI RCATGY 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 1047
BstSLI GKGCMC 1 cut(s) 340
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 1 cut(s) 1618
BstV2I GAAGAC 2 cut(s) 809, 1394
BstX2I RGATCY 2 cut(s) 890, 1025
BstXI CCANNNNNNTGG 1 cut(s) 273
BstYI RGATCY 2 cut(s) 890, 1025
Bsu15I ATCGAT 1 cut(s) 1451
BsuI GTATCC 2 cut(s) 603, 1195
BsuRI GGCC 2 cut(s) 77, 661
BsuTUI ATCGAT 1 cut(s) 1451
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 6 cut(s) 307, 553, 730, 841, 1444, 1444
BtsIMutI CAGTG 5 cut(s) 837, 933, 1075, 1377, 1519
Cac8I GCNNGC 1 cut(s) 169
CaiI CAGNNNCTG 1 cut(s) 1521
CciI TCATGA 1 cut(s) 1087
Cfr9I CCCGGG 1 cut(s) 255
ClaI ATCGAT 1 cut(s) 1451
CseI GACGC 2 cut(s) 1399, 1598
Csp6I GTAC 2 cut(s) 916, 1039
CspCI CAANNNNNGTGG 2 cut(s) 1179, 1214
CviAII CATG 5 cut(s) 736, 1088, 1185, 1298, 1548
CviQI GTAC 2 cut(s) 916, 1039
DdeI CTNAG 6 cut(s) 501, 522, 925, 1218, 1369, 1569
DpnI GATC 5 cut(s) 892, 943, 988, 1027, 1302
DpnII GATC 5 cut(s) 890, 941, 986, 1025, 1300
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 255
EcoRI GAATTC 2 cut(s) 932, 1279
EcoRII CCWGG 4 cut(s) 180, 272, 1506, 1531
EcoT22I ATGCAT 1 cut(s) 1348
FaeI CATG 5 cut(s) 739, 1091, 1188, 1301, 1551
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 2 cut(s) 376, 756
FatI CATG 5 cut(s) 735, 1087, 1184, 1297, 1547
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 132, 1607
FokI GGATG 6 cut(s) 314, 560, 737, 848, 1431, 1451
Fsp4HI GCNGC 2 cut(s) 132, 1607
FspBI CTAG 4 cut(s) 333, 480, 953, 1636
GluI GCNGC 2 cut(s) 132, 1607
GsuI CTGGAG 2 cut(s) 103, 464
HaeIII GGCC 2 cut(s) 77, 661
HapII CCGG 6 cut(s) 256, 435, 528, 608, 662, 1240
HgaI GACGC 2 cut(s) 1399, 1598
Hin1I GRCGYC 1 cut(s) 1391
Hin1II CATG 5 cut(s) 739, 1091, 1188, 1301, 1551
HindIII AAGCTT 2 cut(s) 715, 1173
HinfI GANTC 9 cut(s) 374, 406, 497, 587, 632, 779, 803, 1084, 1367
HpaII CCGG 6 cut(s) 256, 435, 528, 608, 662, 1240
HphI GGTGA 2 cut(s) 589, 1242
Hpy166II GTNNAC 1 cut(s) 1083
Hpy188I TCNGA 7 cut(s) 325, 384, 496, 502, 886, 1033, 1170
Hpy8I GTNNAC 1 cut(s) 1083
HpyAV CCTTC 3 cut(s) 88, 646, 1102
HpyCH4IV ACGT 1 cut(s) 1152
HpyCH4V TGCA 9 cut(s) 162, 171, 319, 705, 980, 1049, 1319, 1346, 1515
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 168, 485, 1492
HpyF3I CTNAG 6 cut(s) 501, 522, 925, 1218, 1369, 1569
HpySE526I ACGT 1 cut(s) 1152
Hsp92I GRCGYC 1 cut(s) 1391
Hsp92II CATG 5 cut(s) 739, 1091, 1188, 1301, 1551
Kzo9I GATC 5 cut(s) 890, 941, 986, 1025, 1300
LmnI GCTCC 3 cut(s) 610, 870, 1483
Lsp1109I GCAGC 1 cut(s) 1618
LweI GCATC 5 cut(s) 171, 466, 967, 1355, 1429
MaeI CTAG 4 cut(s) 333, 480, 953, 1636
MaeII ACGT 1 cut(s) 1152
MaeIII GTNAC 5 cut(s) 412, 554, 1073, 1112, 1517
MalI GATC 5 cut(s) 892, 943, 988, 1027, 1302
MbiI CCGCTC 1 cut(s) 1486
MboI GATC 5 cut(s) 890, 941, 986, 1025, 1300
MfeI CAATTG 2 cut(s) 640, 919
MflI RGATCY 2 cut(s) 890, 1025
MhlI GDGCHC 1 cut(s) 340
MlyI GAGTC 3 cut(s) 812, 1078, 1361
MmeI TCCRAC 1 cut(s) 702
Mph1103I ATGCAT 1 cut(s) 1348
MroXI GAANNNNTTC 3 cut(s) 230, 936, 1002
MseI TTAA 4 cut(s) 651, 786, 1155, 1178
MslI CAYNNNNRTG 1 cut(s) 1382
MspI CCGG 6 cut(s) 256, 435, 528, 608, 662, 1240
MunI CAATTG 2 cut(s) 640, 919
MvaI CCWGG 4 cut(s) 182, 274, 1508, 1533
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 4 cut(s) 137, 168, 485, 1492
NciI CCSGG 6 cut(s) 256, 257, 436, 528, 609, 1241
NdeII GATC 5 cut(s) 890, 941, 986, 1025, 1300
NlaIII CATG 5 cut(s) 739, 1091, 1188, 1301, 1551
NlaIV GGNNCC 1 cut(s) 1526
NmuCI GTSAC 3 cut(s) 554, 1073, 1517
NsiI ATGCAT 1 cut(s) 1348
NspI RCATGY 1 cut(s) 739
NspV TTCGAA 1 cut(s) 823
PagI TCATGA 1 cut(s) 1087
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 735
PdmI GAANNNNTTC 3 cut(s) 230, 936, 1002
PfeI GAWTC 6 cut(s) 374, 406, 497, 587, 632, 779
PflFI GACNNNGTC 1 cut(s) 388
PfoI TCCNGGA 2 cut(s) 272, 434
PkrI GCNGC 2 cut(s) 133, 1608
Ple19I CGATCG 1 cut(s) 944
PleI GAGTC 3 cut(s) 811, 1078, 1361
PpsI GAGTC 3 cut(s) 811, 1078, 1361
PscI ACATGT 1 cut(s) 735
Psp1406I AACGTT 1 cut(s) 1152
Psp6I CCWGG 4 cut(s) 180, 272, 1506, 1531
PspGI CCWGG 4 cut(s) 180, 272, 1506, 1531
PspN4I GGNNCC 1 cut(s) 1526
PstI CTGCAG 1 cut(s) 1051
PstNI CAGNNNCTG 1 cut(s) 1521
PsuI RGATCY 2 cut(s) 890, 1025
PsyI GACNNNGTC 1 cut(s) 388
PvuI CGATCG 1 cut(s) 944
RsaI GTAC 2 cut(s) 917, 1040
RsaNI GTAC 2 cut(s) 916, 1039
RseI CAYNNNNRTG 1 cut(s) 1382
SaqAI TTAA 4 cut(s) 651, 786, 1155, 1178
SatI GCNGC 2 cut(s) 132, 1607
Sau3AI GATC 5 cut(s) 890, 941, 986, 1025, 1300
SchI GAGTC 3 cut(s) 812, 1078, 1361
SduI GDGCHC 1 cut(s) 340
SfaNI GCATC 5 cut(s) 171, 466, 967, 1355, 1429
SfcI CTRYAG 1 cut(s) 1047
SfuI TTCGAA 1 cut(s) 823
SmaI CCCGGG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 1382
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 6 cut(s) 5, 129, 132, 293, 596, 1486
SspI AATATT 3 cut(s) 287, 1362, 1398
SspMI CTAG 4 cut(s) 333, 480, 953, 1636
StuI AGGCCT 1 cut(s) 77
TaiI ACGT 1 cut(s) 1155
TaqI TCGA 6 cut(s) 461, 542, 823, 940, 1451, 1579
TatI WGTACW 2 cut(s) 915, 1038
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 6 cut(s) 374, 406, 497, 587, 632, 779
Tru1I TTAA 4 cut(s) 651, 786, 1155, 1178
Tru9I TTAA 4 cut(s) 651, 786, 1155, 1178
TscAI CASTG 5 cut(s) 837, 933, 1082, 1384, 1526
TseFI GTSAC 3 cut(s) 554, 1073, 1517
TseI GCWGC 1 cut(s) 1606
Tsp45I GTSAC 3 cut(s) 554, 1073, 1517
TspDTI ATGAA 3 cut(s) 549, 645, 1400
TspMI CCCGGG 1 cut(s) 255
TspRI CASTG 5 cut(s) 837, 933, 1082, 1384, 1526
Tth111I GACNNNGTC 1 cut(s) 388
XapI RAATTY 6 cut(s) 536, 826, 875, 932, 1279, 1468
XceI RCATGY 1 cut(s) 739
XmaI CCCGGG 1 cut(s) 255
XmnI GAANNNNTTC 3 cut(s) 230, 936, 1002
XspI CTAG 4 cut(s) 333, 480, 953, 1636
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.