MD11G1015200.v1.1

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001307
Physical Location & Seq
Forward (+)
1207393 .. 1209609
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1015200.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGAGCATCACAAGCCCTCCTTCCACGAAGCACTCATCGCCGACGAAGCCAGGGCCAAAGGTCCCTTCACTTTCAATCTTGTTCTCCAAAAGAAAATCCATGGCCTTCACTTATGTATTCACGTTTGCCTTCATTGCCTGCACTTTCTTTTTGGTTTTCAACCCGTCACGCCAAAATGGCCCTCTTCCTCTTCGTTTCAAGAACCTCCTCCATGCCTCCTTCTACTCCTCTTCTCAAAACCCTAATGCACACCTGGAGCTTTCCCGGAGAGACCCCTTTTCTGGAAAACCGAATTTTTCGCCGCGGTTTGATGAGAATGCAAGGTTGAAGGATGATAAGGTGGATAATCAAGAGATTGGTTTCTCTCAACTTCCGGAGAAAAACGAAAGCTTGAAGAGAGATAACAATGCTCAGTCATCCAACCCCATTCTTTCATCGTCCAATAACACAGAAATCGAAGTTTCTGGGAAAATGGAGGGTTCCAAGGGAAGAAACGAGACCAAGAAGCACACACATTCACATTCTTCTTCTTCGAAAGATTCAAATGGCAAACGTGGCTCGAAGAAGAAAAGTGATCATAAAAAGGTGTCATCTAAGAAGCAAGGGAAACAGAGTGAGTTGAAGTCCAAGCTGATGAATGGCTGTGACATATTTGATGGAAGATGGGTGAGAGATAACTCATACCCGCTTTATGCTCCAGGGTCTTGTCCTTGGATCGATGAGCCTTTCGATTGTTTTCTAAATGGAAGGCCGGACAATGGCTACGAGAGGTATAGATGGCAACCAAAACGTTGCAATATCCCAAGGTTGAATGGTAAGAAGATGTTGAGATTGTTGACAGGGAAGCGTCTAGTTTTTGTTGGCGATTCTCTCAACAGGAATATGTGGGAGTCTTTGCTATGTATTCTTAGGAACTCAGTGGATAATAAGAACAAAGTTTATGAAGTTTCTGGTAGGCAAGAATTTCGCACAGAAGGCTCTTACTCTTTTATTTTCGAGGAGTATAATTGTTCCGTGGAGTTCTTTCAATCCCGGTTCCTAGTTCAAGAATGGGAGATGCCAGAACCAAGTGGATCAAAGAAGGAAACGCTTCGCATTGATTTGATTGAGAGATCATCCAACAACTACAAAAATGCAGATGTTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTTAAGCGGGAAGGGTTACTATCAAGAGGGTTCCCATATCTACGGTGAATTGGATGTTGATGATGCATTTCGCAAAGCTTTATCGACATGGGCTAGATGGATTGACACCAATGTAAATCCTAAGAAAACCGCTGTTTTCTTCCGGGGCTATTCACCTTCTCACTTTAGAGGTGGAGAATGGAATTCTGGAGGGCATTGCCATGGTGAAACTAAGCCAACTACAAGCTTGGAATCCACGGAATATGGAGGAGAATATCCAACGAACATGAAGATCCTAGACTCGGTACTCAAGGGGATGAAGACGCCAGTCAACTATCTGAACATTACAACAATGACTGATTTTCGGAAGGATGCTCATCCATCGATTTACAGAAAGCCGAATTTAACTGAGGAGGAGCGAAAACCGCCTATGATCCAGGATTGCAGTCACTGGTGCCTCCCTGGCGTTCCTGATACATGGAACGAGCTTATATATGCTCAACTCCTCAAACGTAACCAGAAACAACATAGAGAGAAGAAACAGAAAAACCAGAGGACAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

577

Amino Acids

66.27

Weight (kDa)

9.47

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 214 - 266 4e-22 PMR5 N terminal Domain
PC-Esterase PF13839 267 - 554 2.6e-102 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1617
AccII CGCG 1 cut(s) 304
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 6 cut(s) 302, 304, 686, 1191, 1314, 1589
AclI AACGTT 1 cut(s) 790
AclWI GGATC 4 cut(s) 722, 1081, 1450, 1591
AcsI RAATTY 4 cut(s) 292, 962, 1366, 1564
AcyI GRCGYC 1 cut(s) 1487
AfaI GTAC 1 cut(s) 1470
AfiI CCNNNNNNNGG 3 cut(s) 281, 758, 1465
AjnI CCWGG 5 cut(s) 49, 252, 697, 1599, 1624
AjuI GAANNNNNNNTTGG 2 cut(s) 49, 81
AluBI AGCT 6 cut(s) 259, 390, 631, 1262, 1410, 1651
AluI AGCT 6 cut(s) 259, 390, 631, 1262, 1410, 1651
Alw26I GTCTC 2 cut(s) 264, 491
AlwI GGATC 4 cut(s) 722, 1081, 1450, 1591
AlwNI CAGNNNCTG 1 cut(s) 1614
Aor13HI TCCGGA 1 cut(s) 373
AoxI GGCC 4 cut(s) 53, 102, 178, 749
ApoI RAATTY 4 cut(s) 292, 962, 1366, 1564
Asp700I GAANNNNTTC 1 cut(s) 1089
AspS9I GGNCC 3 cut(s) 53, 61, 179
AsuC2I CCSGG 3 cut(s) 265, 1033, 1328
AsuHPI GGTGA 4 cut(s) 679, 1241, 1329, 1400
AsuII TTCGAA 1 cut(s) 533
AvaII GGWCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 1617
BbsI GAAGAC 1 cut(s) 1490
BccI CCATC 5 cut(s) 650, 657, 771, 1275, 1552
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 5 cut(s) 51, 254, 699, 1601, 1626
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 3 cut(s) 265, 1033, 1328
BcoDI GTCTC 2 cut(s) 264, 491
BfaI CTAG 4 cut(s) 851, 1040, 1278, 1460
BglI GCCNNNNNGGC 2 cut(s) 177, 1626
BisI GCNGC 1 cut(s) 302
BlsI GCNGC 1 cut(s) 303
Bme1390I CCNGG 8 cut(s) 51, 254, 265, 699, 1033, 1328, 1601, 1626
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 53, 61, 179
BmiI GGNNCC 5 cut(s) 63, 481, 1037, 1216, 1619
BmrFI CCNGG 8 cut(s) 51, 254, 265, 699, 1033, 1328, 1601, 1626
BmsI GCATC 4 cut(s) 15, 1047, 1237, 1525
BoxI GACNNNNGTC 1 cut(s) 1490
BpiI GAAGAC 1 cut(s) 1490
BpmI CTGGAG 3 cut(s) 275, 681, 1392
Bpu14I TTCGAA 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 1457
BpuMI CCSGG 3 cut(s) 265, 1033, 1328
Bsa29I ATCGAT 2 cut(s) 717, 1547
BsaBI GATNNNNATC 1 cut(s) 1539
BsaHI GRCGYC 1 cut(s) 1487
BsaI GGTCTC 2 cut(s) 264, 491
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 758, 1465
Bse1I ACTGG 3 cut(s) 1169, 1490, 1619
Bse3DI GCAATG 2 cut(s) 132, 1378
Bse8I GATNNNNATC 1 cut(s) 1539
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 5 cut(s) 51, 254, 699, 1601, 1626
BseCI ATCGAT 2 cut(s) 717, 1547
BseGI GGATG 7 cut(s) 337, 416, 1115, 1243, 1485, 1540, 1540
BseJI GATNNNNATC 1 cut(s) 1539
BseLI CCNNNNNNNGG 3 cut(s) 281, 758, 1465
BseMI GCAATG 2 cut(s) 132, 1378
BseMII CTCAG 3 cut(s) 425, 930, 1563
BseNI ACTGG 3 cut(s) 1169, 1490, 1619
BseRI GAGGAG 7 cut(s) 197, 217, 1013, 1446, 1589, 1592, 1658
BsgI GTGCAG 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 4 cut(s) 55, 104, 180, 751
BshNI GGYRCC 1 cut(s) 1617
BshVI ATCGAT 2 cut(s) 717, 1547
BsiSI CCGG 5 cut(s) 265, 374, 752, 1033, 1327
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 3 cut(s) 281, 758, 1465
BsmAI GTCTC 2 cut(s) 264, 491
BsmFI GGGAC 1 cut(s) 47
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 4 cut(s) 55, 104, 180, 751
Bso31I GGTCTC 2 cut(s) 264, 491
Bsp119I TTCGAA 1 cut(s) 533
Bsp13I TCCGGA 1 cut(s) 373
Bsp143I GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
Bsp19I CCATGG 2 cut(s) 99, 1384
BspACI CCGC 6 cut(s) 302, 304, 686, 1191, 1314, 1589
BspANI GGCC 4 cut(s) 55, 104, 180, 751
BspCNI CTCAG 3 cut(s) 424, 929, 1564
BspDI ATCGAT 2 cut(s) 717, 1547
BspEI TCCGGA 1 cut(s) 373
BspFNI CGCG 1 cut(s) 304
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 5 cut(s) 63, 481, 1037, 1216, 1619
BspPI GGATC 4 cut(s) 722, 1081, 1450, 1591
BspT104I TTCGAA 1 cut(s) 533
BspT107I GGYRCC 1 cut(s) 1617
BspTNI GGTCTC 2 cut(s) 264, 491
BsrDI GCAATG 2 cut(s) 132, 1378
BsrI ACTGG 3 cut(s) 1169, 1490, 1619
BssMI GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
BssNI GRCGYC 1 cut(s) 1487
BssT1I CCWWGG 5 cut(s) 99, 483, 710, 803, 1384
Bst2UI CCWGG 5 cut(s) 51, 254, 699, 1601, 1626
Bst4CI ACNGT 1 cut(s) 1229
Bst6I CTCTTC 4 cut(s) 189, 195, 235, 389
BstACI GRCGYC 1 cut(s) 1487
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 8 cut(s) 411, 594, 908, 916, 1305, 1395, 1572, 1728
BstDSI CCRYGG 5 cut(s) 99, 302, 1014, 1384, 1419
BstF5I GGATG 7 cut(s) 337, 416, 1115, 1243, 1485, 1540, 1540
BstFNI CGCG 1 cut(s) 304
BstKTI GATC 6 cut(s) 577, 717, 1076, 1115, 1458, 1599
BstMAI GTCTC 2 cut(s) 264, 491
BstMBI GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
BstMWI GCNNNNNNNGC 9 cut(s) 12, 37, 46, 134, 177, 555, 975, 1588, 1626
BstNI CCWGG 5 cut(s) 51, 254, 699, 1601, 1626
BstPAI GACNNNNGTC 1 cut(s) 1490
BstSCI CCNGG 8 cut(s) 49, 252, 263, 697, 1031, 1326, 1599, 1624
BstUI CGCG 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 1490
BstX2I RGATCY 1 cut(s) 1455
BstYI RGATCY 1 cut(s) 1455
Bsu15I ATCGAT 2 cut(s) 717, 1547
BsuRI GGCC 4 cut(s) 55, 104, 180, 751
BsuTUI ATCGAT 2 cut(s) 717, 1547
BtgI CCRYGG 5 cut(s) 99, 302, 1014, 1384, 1419
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 7 cut(s) 337, 416, 1115, 1243, 1485, 1540, 1540
BtsIMutI CAGTG 3 cut(s) 924, 1162, 1612
Cac8I GCNNGC 1 cut(s) 139
CaiI CAGNNNCTG 1 cut(s) 1614
CciI TCATGA 1 cut(s) 1174
Cfr13I GGNCC 3 cut(s) 53, 61, 179
Cfr42I CCGCGG 1 cut(s) 305
ClaI ATCGAT 2 cut(s) 717, 1547
CseI GACGC 2 cut(s) 836, 1495
Csp6I GTAC 1 cut(s) 1469
CviAII CATG 7 cut(s) 100, 212, 1175, 1272, 1385, 1450, 1641
CviQI GTAC 1 cut(s) 1469
DdeI CTNAG 8 cut(s) 411, 594, 908, 916, 1305, 1395, 1572, 1728
DpnI GATC 6 cut(s) 576, 716, 1075, 1114, 1457, 1598
DpnII GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
Eam1104I CTCTTC 4 cut(s) 189, 195, 235, 389
EarI CTCTTC 4 cut(s) 189, 195, 235, 389
Eco130I CCWWGG 5 cut(s) 99, 483, 710, 803, 1384
Eco31I GGTCTC 2 cut(s) 264, 491
Eco47I GGWCC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 5 cut(s) 49, 252, 697, 1599, 1624
EcoT14I CCWWGG 5 cut(s) 99, 483, 710, 803, 1384
EcoT22I ATGCAT 1 cut(s) 1252
ErhI CCWWGG 5 cut(s) 99, 483, 710, 803, 1384
FaeI CATG 7 cut(s) 103, 215, 1178, 1275, 1388, 1453, 1644
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 1 cut(s) 47
FatI CATG 7 cut(s) 99, 211, 1174, 1271, 1384, 1449, 1640
FauI CCCGC 2 cut(s) 693, 1184
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 302
FokI GGATG 7 cut(s) 344, 403, 1102, 1250, 1492, 1527, 1547
Fsp4HI GCNGC 1 cut(s) 302
FspBI CTAG 4 cut(s) 851, 1040, 1278, 1460
GluI GCNGC 1 cut(s) 302
GsuI CTGGAG 3 cut(s) 275, 681, 1392
HaeIII GGCC 4 cut(s) 55, 104, 180, 751
HapII CCGG 5 cut(s) 265, 374, 752, 1033, 1327
HgaI GACGC 2 cut(s) 836, 1495
Hin1I GRCGYC 1 cut(s) 1487
Hin1II CATG 7 cut(s) 103, 215, 1178, 1275, 1388, 1453, 1644
HincII GTYRAC 2 cut(s) 837, 1495
HindII GTYRAC 2 cut(s) 837, 1495
HindIII AAGCTT 3 cut(s) 388, 1260, 1408
HinfI GANTC 6 cut(s) 539, 866, 890, 1171, 1415, 1463
HpaII CCGG 5 cut(s) 265, 374, 752, 1033, 1327
HphI GGTGA 4 cut(s) 679, 1241, 1329, 1400
Hpy166II GTNNAC 3 cut(s) 837, 1170, 1495
Hpy188I TCNGA 2 cut(s) 1503, 1530
Hpy188III TCNNGA 9 cut(s) 199, 282, 350, 374, 1046, 1175, 1208, 1371, 1634
Hpy8I GTNNAC 3 cut(s) 837, 1170, 1495
Hpy99I CGWCG 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 1229
HpyCH4IV ACGT 4 cut(s) 122, 553, 790, 1675
HpyCH4V TGCA 7 cut(s) 141, 248, 320, 795, 1136, 1250, 1608
HpyF10VI GCNNNNNNNGC 9 cut(s) 12, 37, 46, 134, 177, 555, 975, 1588, 1626
HpyF3I CTNAG 8 cut(s) 411, 594, 908, 916, 1305, 1395, 1572, 1728
HpySE526I ACGT 4 cut(s) 122, 553, 790, 1675
Hsp92I GRCGYC 1 cut(s) 1487
Hsp92II CATG 7 cut(s) 103, 215, 1178, 1275, 1388, 1453, 1644
Kpn2I TCCGGA 1 cut(s) 373
Ksp22I TGATCA 1 cut(s) 574
KspI CCGCGG 1 cut(s) 305
Kzo9I GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
LmnI GCTCC 3 cut(s) 256, 700, 1579
LweI GCATC 4 cut(s) 15, 1047, 1237, 1525
MaeI CTAG 4 cut(s) 851, 1040, 1278, 1460
MaeII ACGT 4 cut(s) 122, 553, 790, 1675
MaeIII GTNAC 6 cut(s) 165, 644, 1160, 1199, 1610, 1676
MalI GATC 6 cut(s) 576, 716, 1075, 1114, 1457, 1598
MboI GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
MflI RGATCY 1 cut(s) 1455
MluCI AATT 6 cut(s) 292, 962, 1006, 1232, 1366, 1564
MlyI GAGTC 3 cut(s) 899, 1165, 1457
MmeI TCCRAC 3 cut(s) 444, 1143, 1466
Mph1103I ATGCAT 1 cut(s) 1252
MroI TCCGGA 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 1089
MseI TTAA 2 cut(s) 1187, 1568
MslI CAYNNNNRTG 1 cut(s) 1383
MspA1I CMGCKG 2 cut(s) 304, 1316
MspI CCGG 5 cut(s) 265, 374, 752, 1033, 1327
MspR9I CCNGG 8 cut(s) 51, 254, 265, 699, 1033, 1328, 1601, 1626
Mva1269I GAATGC 1 cut(s) 322
MvaI CCWGG 5 cut(s) 51, 254, 699, 1601, 1626
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 9 cut(s) 12, 37, 46, 134, 177, 555, 975, 1588, 1626
NciI CCSGG 3 cut(s) 265, 1033, 1328
NcoI CCATGG 2 cut(s) 99, 1384
NdeII GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
NlaIII CATG 7 cut(s) 103, 215, 1178, 1275, 1388, 1453, 1644
NlaIV GGNNCC 5 cut(s) 63, 481, 1037, 1216, 1619
NmuCI GTSAC 4 cut(s) 165, 644, 1160, 1610
NsiI ATGCAT 1 cut(s) 1252
NspV TTCGAA 1 cut(s) 533
PagI TCATGA 1 cut(s) 1174
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 3 cut(s) 539, 866, 1415
PfoI TCCNGGA 2 cut(s) 263, 1599
PkrI GCNGC 1 cut(s) 303
PleI GAGTC 3 cut(s) 898, 1165, 1457
PpsI GAGTC 3 cut(s) 898, 1165, 1457
PpuMI RGGWCCY 1 cut(s) 61
PshAI GACNNNNGTC 1 cut(s) 1490
Psp1406I AACGTT 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 5 cut(s) 49, 252, 697, 1599, 1624
PspGI CCWGG 5 cut(s) 49, 252, 697, 1599, 1624
PspN4I GGNNCC 5 cut(s) 63, 481, 1037, 1216, 1619
PspPI GGNCC 3 cut(s) 53, 61, 179
PspPPI RGGWCCY 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 1614
PsuI RGATCY 1 cut(s) 1455
RsaI GTAC 1 cut(s) 1470
RsaNI GTAC 1 cut(s) 1469
RseI CAYNNNNRTG 1 cut(s) 1383
SacII CCGCGG 1 cut(s) 305
SaqAI TTAA 2 cut(s) 1187, 1568
SatI GCNGC 1 cut(s) 302
Sau3AI GATC 6 cut(s) 574, 714, 1073, 1112, 1455, 1596
Sau96I GGNCC 3 cut(s) 53, 61, 179
SchI GAGTC 3 cut(s) 899, 1165, 1457
ScrFI CCNGG 8 cut(s) 51, 254, 265, 699, 1033, 1328, 1601, 1626
SfaNI GCATC 4 cut(s) 15, 1047, 1237, 1525
Sfr303I CCGCGG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 533
SgrBI CCGCGG 1 cut(s) 305
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 1383
SmlI CTYRAG 1 cut(s) 1472
SmoI CTYRAG 1 cut(s) 1472
Sse9I AATT 6 cut(s) 292, 962, 1006, 1232, 1366, 1564
SsiI CCGC 6 cut(s) 302, 304, 686, 1191, 1314, 1589
SspMI CTAG 4 cut(s) 851, 1040, 1278, 1460
StyD4I CCNGG 8 cut(s) 49, 252, 263, 697, 1031, 1326, 1599, 1624
StyI CCWWGG 5 cut(s) 99, 483, 710, 803, 1384
TaaI ACNGT 1 cut(s) 1229
TaiI ACGT 4 cut(s) 125, 556, 793, 1678
TaqI TCGA 8 cut(s) 456, 533, 560, 717, 729, 996, 1268, 1547
TasI AATT 6 cut(s) 292, 962, 1006, 1232, 1366, 1564
TauI GCSGC 1 cut(s) 304
TfiI GAWTC 3 cut(s) 539, 866, 1415
Tru1I TTAA 2 cut(s) 1187, 1568
Tru9I TTAA 2 cut(s) 1187, 1568
TscAI CASTG 3 cut(s) 924, 1169, 1619
TseFI GTSAC 4 cut(s) 165, 644, 1160, 1610
Tsp45I GTSAC 4 cut(s) 165, 644, 1160, 1610
TspDTI ATGAA 6 cut(s) 121, 423, 650, 957, 1466, 1496
TspGWI ACGGA 2 cut(s) 1003, 1436
TspRI CASTG 3 cut(s) 924, 1169, 1619
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 4 cut(s) 292, 962, 1366, 1564
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 4 cut(s) 851, 1040, 1278, 1460
Zsp2I ATGCAT 1 cut(s) 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.