Rw0G002570

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00078
OG0001307
Physical Location & Seq
Reverse (-)
39468 .. 41789
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002570.1

Sequence Viewer

Length: 1815 bp
ATGGCGGATTGGGGACATTCCTCAACAACAGAACACCTCTTACCAACATCCCTAGGAGAGTCGAAAGCCTCTCCTAGGCCTTCACCGACACCAGCAAGGCCTCAATGTTTTCAATCTTCTTCTCCAAGAGAAATTCCATGGTTTTGTACTTATGCATTCACAATTGTCTTCATTACATGTACCATCCTCTTGGTTTTCAACCCTTCTCGCCCTTCGGTTCCTTTTGGCTTCAAAAATCTTCTTTATACCTCTCCTCAGACCCCAAACCCTCATTGGAAACATGATTCTTTACCACAGTTTGATGAGATTGGAAGTTTGAAAGAGAATGTGGGGATTGATCAGAAGGGATTTCAGATTGGTCTTAATCAATCTTCTGGGGGAAATGGAGGAACCAAGAAAAATGTGGAGACTGAAAGGGATGATCATGTTGGTGCTCCTCAACCATCATACACTACCAAGAAAAATGTAGAGACTGAAAGGGATGATCATATTGGTGCTCCTCAACCATCAAACACTACCATGGAAAATGTAGAGATTAAAAGGGATGATCATATTGGTACTTCTCAATCATCAAACACTACCATTCTTTCCATGTTCAAAGACTCTAATAACACAGGAAACGAAGTTTCTGAGAAAGTTGAGAGTTCTGAGAGAAGAAATGAGACCAAGAATGCTGTAAGCTTTCATTCTTCTTCTAATGGAGAAAATGGTTTGAAGGAGAAAGGGAGTGATAGTAAAAAGGCACCATCTAACAGAAACAAACTGGATAATTCTTACCCACTTTATGCTCCAGGAACTTGTCCTTATATAGATGAGCCTTTCGATTGTTTCCACAACGGTAGGCCTGATAATGGTTACGAGAAGTACAGATGGCAACCGAAACATTGCAATGTCCCAAGGTTGGATGGTAAGAAGATGTTGAGATTGTTGGCAGGAAAGCGTCTAGTTTTTGTTGGCGATTCTCTCAATAGGAATATGTGGGAGTCTTTGCTTTGTGTTCTTAGAAATTCAGTGGAAGATAAGAACAAAGTTTATGAAGTCTCGGGCAGGCAAGAGTTCCGTACAGAGGACTCTTTCTCTTTCATATTCCAGGATTATAATTGTTCAGTAGAGTTCTTTCAATCACAATTTATAGTTCAAGAATGGGAGGGGAAAGACAAAAATGGATCCAAAAAGGAAACCCTTCGCCTTGATTTAATGGAGAGTTCCGCGGATAAATACAAAGATGCAGATATTCTCATCTTCAACACAGGTCACTGGTGGACTCATGAGAAAACTTCCAACGGGTTGGGTTACTATCAAGAAGGCAGCCATGTCTATGATGAATTGAATGGAGAAGAGGCATATCGGAGGGCTTTAGCAACATGGGCAAGATGGGTGGATACCAAAGTAGATCCTAAGAAAACTGCTGTTTTCTTCCGCGGCTATTCACCTTCTCACTTTAGGGGTGGAGCATGGAACTCTGGAGGGCAATGCCACGGCGAGACAAAACCAACTACAGAAACTGAATACGGAGGAGAATATTATCCATCACAAGTGAAGATCTTGGACTCAGTGATGGAGAAGATGATGACTCCAATCTTCTATCTGAACATCACAGCAATGACTGATTTCCGGAAGGATGCTCATCCATCGGTTTACAGAATGCCCAACTTAACTGCAGAGGAAAGAAAAGCGACTTCAATCCAAGATTGCAGCCACTGGTGCCTACCTGGTGTGCCTGAAACATGGAACGAGCTTGTATATGCTCAACTCCTTAGACGCAACATAAAGCAACAGAAGAAGAAGCAACATCAACAGAAGCGTCAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

604

Amino Acids

68.9

Weight (kDa)

7.01

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 256 - 297 2.1e-15 PMR5 N terminal Domain
PC-Esterase PF13839 298 - 583 1e-99 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
AccB1I GGYRCC 2 cut(s) 742, 1704
AccII CGCG 2 cut(s) 1211, 1422
AccIII TCCGGA 1 cut(s) 1614
AciI CCGC 5 cut(s) 5, 1209, 1211, 1420, 1422
AclWI GGATC 3 cut(s) 1161, 1174, 1388
AcsI RAATTY 2 cut(s) 132, 1006
AfaI GTAC 5 cut(s) 148, 181, 559, 866, 1063
AfiI CCNNNNNNNGG 4 cut(s) 75, 851, 901, 1066
AflIII ACRYGT 1 cut(s) 176
AjnI CCWGG 3 cut(s) 790, 1089, 1711
AluBI AGCT 2 cut(s) 681, 1738
AluI AGCT 2 cut(s) 681, 1738
Alw21I GWGCWC 2 cut(s) 436, 499
Alw26I GTCTC 5 cut(s) 401, 464, 656, 1045, 1478
AlwI GGATC 3 cut(s) 1161, 1174, 1388
AlwNI CAGNNNCTG 2 cut(s) 1505, 1701
Ama87I CYCGRG 1 cut(s) 1042
Aor13HI TCCGGA 1 cut(s) 1614
AoxI GGCC 3 cut(s) 77, 98, 842
ApeKI GCWGC 2 cut(s) 1308, 1695
ApoI RAATTY 2 cut(s) 132, 1006
Asp700I GAANNNNTTC 1 cut(s) 1182
AspA2I CCTAGG 2 cut(s) 52, 74
AsuHPI GGTGA 2 cut(s) 75, 1422
AvaI CYCGRG 1 cut(s) 1042
AvrII CCTAGG 2 cut(s) 52, 74
BaeI ACNNNNGTAYC 2 cut(s) 1374, 1407
BamHI GGATCC 1 cut(s) 1166
BanI GGYRCC 2 cut(s) 742, 1704
BbsI GAAGAC 1 cut(s) 160
Bbv12I GWGCWC 2 cut(s) 436, 499
BbvI GCAGC 2 cut(s) 1320, 1707
BceAI ACGGC 1 cut(s) 1495
BciT130I CCWGG 3 cut(s) 792, 1091, 1713
BciVI GTATCC 1 cut(s) 1375
BclI TGATCA 4 cut(s) 337, 421, 484, 547
BcoDI GTCTC 5 cut(s) 401, 464, 656, 1045, 1478
BfaI CTAG 3 cut(s) 53, 75, 944
BfmI CTRYAG 2 cut(s) 1497, 1659
BfuI GTATCC 1 cut(s) 1375
BglII AGATCT 1 cut(s) 1542
BisI GCNGC 3 cut(s) 1309, 1423, 1696
BlnI CCTAGG 2 cut(s) 52, 74
BlsI GCNGC 3 cut(s) 1310, 1424, 1697
Bme1390I CCNGG 3 cut(s) 792, 1091, 1713
BmeT110I CYCGRG 1 cut(s) 1042
BmiI GGNNCC 5 cut(s) 219, 391, 744, 1168, 1706
BmrFI CCNGG 3 cut(s) 792, 1091, 1713
BmsI GCATC 2 cut(s) 1216, 1612
BpiI GAAGAC 1 cut(s) 160
BpmI CTGGAG 2 cut(s) 774, 1485
BsaBI GATNNNNATC 1 cut(s) 1626
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 8 cut(s) 52, 74, 137, 519, 896, 1209, 1420, 1477
BsaWI WCCGGW 1 cut(s) 1614
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 851, 901, 1066
Bse1I ACTGG 3 cut(s) 768, 1262, 1706
Bse3DI GCAATG 4 cut(s) 883, 895, 1478, 1608
Bse8I GATNNNNATC 1 cut(s) 1626
BseAI TCCGGA 1 cut(s) 1614
BseBI CCWGG 3 cut(s) 792, 1091, 1713
BseDI CCNNGG 8 cut(s) 52, 74, 137, 519, 896, 1209, 1420, 1477
BseGI GGATG 8 cut(s) 47, 183, 424, 487, 550, 910, 1627, 1627
BseJI GATNNNNATC 1 cut(s) 1626
BseLI CCNNNNNNNGG 4 cut(s) 75, 851, 901, 1066
BseMI GCAATG 4 cut(s) 883, 895, 1478, 1608
BseMII CTCAG 4 cut(s) 269, 621, 639, 1566
BseNI ACTGG 3 cut(s) 768, 1262, 1706
BseRI GAGGAG 4 cut(s) 243, 426, 489, 1530
BseXI GCAGC 2 cut(s) 1320, 1707
Bsh1236I CGCG 2 cut(s) 1211, 1422
BshFI GGCC 3 cut(s) 79, 100, 844
BshNI GGYRCC 2 cut(s) 742, 1704
BsiHKAI GWGCWC 2 cut(s) 436, 499
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 1615
BslFI GGGAC 2 cut(s) 27, 878
BslI CCNNNNNNNGG 4 cut(s) 75, 851, 901, 1066
BsmAI GTCTC 5 cut(s) 401, 464, 656, 1045, 1478
BsmFI GGGAC 2 cut(s) 27, 878
BsmI GAATGC 3 cut(s) 155, 676, 1650
BsnI GGCC 3 cut(s) 79, 100, 844
Bso31I GGTCTC 1 cut(s) 656
BsoBI CYCGRG 1 cut(s) 1042
Bsp1286I GDGCHC 2 cut(s) 436, 499
Bsp13I TCCGGA 1 cut(s) 1614
Bsp143I GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
Bsp19I CCATGG 2 cut(s) 137, 519
BspACI CCGC 5 cut(s) 5, 1209, 1211, 1420, 1422
BspANI GGCC 3 cut(s) 79, 100, 844
BspCNI CTCAG 4 cut(s) 268, 622, 640, 1565
BspEI TCCGGA 1 cut(s) 1614
BspFNI CGCG 2 cut(s) 1211, 1422
BspHI TCATGA 1 cut(s) 1267
BspLI GGNNCC 5 cut(s) 219, 391, 744, 1168, 1706
BspMAI CTGCAG 1 cut(s) 1663
BspPI GGATC 3 cut(s) 1161, 1174, 1388
BspT107I GGYRCC 2 cut(s) 742, 1704
BspTNI GGTCTC 1 cut(s) 656
BsrDI GCAATG 4 cut(s) 883, 895, 1478, 1608
BsrI ACTGG 3 cut(s) 768, 1262, 1706
BssECI CCNNGG 8 cut(s) 52, 74, 137, 519, 896, 1209, 1420, 1477
BssMI GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
BssT1I CCWWGG 5 cut(s) 52, 74, 137, 519, 896
Bst2UI CCWGG 3 cut(s) 792, 1091, 1713
Bst4CI ACNGT 2 cut(s) 297, 839
Bst6I CTCTTC 1 cut(s) 1332
BstC8I GCNNGC 1 cut(s) 1049
BstDEI CTNAG 7 cut(s) 255, 630, 648, 1001, 1398, 1552, 1757
BstDSI CCRYGG 5 cut(s) 137, 519, 1209, 1420, 1477
BstENI CCTNNNNNAGG 1 cut(s) 73
BstF5I GGATG 8 cut(s) 47, 183, 424, 487, 550, 910, 1627, 1627
BstFNI CGCG 2 cut(s) 1211, 1422
BstKTI GATC 7 cut(s) 340, 424, 487, 550, 1169, 1396, 1545
BstMAI GTCTC 5 cut(s) 401, 464, 656, 1045, 1478
BstMBI GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
BstMWI GCNNNNNNNGC 2 cut(s) 1367, 1704
BstNI CCWGG 3 cut(s) 792, 1091, 1713
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 3 cut(s) 790, 1089, 1711
BstSFI CTRYAG 2 cut(s) 1497, 1659
BstUI CGCG 2 cut(s) 1211, 1422
BstV1I GCAGC 2 cut(s) 1320, 1707
BstV2I GAAGAC 1 cut(s) 160
BstX2I RGATCY 3 cut(s) 1166, 1393, 1542
BstXI CCANNNNNNTGG 2 cut(s) 190, 1288
BstYI RGATCY 3 cut(s) 1166, 1393, 1542
BsuI GTATCC 1 cut(s) 1375
BsuRI GGCC 3 cut(s) 79, 100, 844
BtgI CCRYGG 5 cut(s) 137, 519, 1209, 1420, 1477
BtsCI GGATG 8 cut(s) 47, 183, 424, 487, 550, 910, 1627, 1627
BtsIMutI CAGTG 4 cut(s) 1017, 1255, 1560, 1699
Cac8I GCNNGC 1 cut(s) 1049
CaiI CAGNNNCTG 2 cut(s) 1505, 1701
CciI TCATGA 1 cut(s) 1267
Cfr42I CCGCGG 2 cut(s) 1212, 1423
CseI GACGC 3 cut(s) 929, 1770, 1793
CsiI ACCWGGT 1 cut(s) 1711
Csp6I GTAC 5 cut(s) 147, 180, 558, 865, 1062
CspCI CAANNNNNGTGG 2 cut(s) 1359, 1394
CviQI GTAC 5 cut(s) 147, 180, 558, 865, 1062
DdeI CTNAG 7 cut(s) 255, 630, 648, 1001, 1398, 1552, 1757
DpnI GATC 7 cut(s) 339, 423, 486, 549, 1168, 1395, 1544
DpnII GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
Eam1104I CTCTTC 1 cut(s) 1332
EarI CTCTTC 1 cut(s) 1332
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 5 cut(s) 52, 74, 137, 519, 896
Eco147I AGGCCT 3 cut(s) 79, 100, 844
Eco31I GGTCTC 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 1042
EcoNI CCTNNNNNAGG 1 cut(s) 73
EcoRII CCWGG 3 cut(s) 790, 1089, 1711
EcoT14I CCWWGG 5 cut(s) 52, 74, 137, 519, 896
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 5 cut(s) 52, 74, 137, 519, 896
FaqI GGGAC 2 cut(s) 27, 878
FbaI TGATCA 4 cut(s) 337, 421, 484, 547
Fnu4HI GCNGC 3 cut(s) 1309, 1423, 1696
FokI GGATG 8 cut(s) 34, 170, 431, 494, 557, 917, 1614, 1634
Fsp4HI GCNGC 3 cut(s) 1309, 1423, 1696
FspBI CTAG 3 cut(s) 53, 75, 944
GluI GCNGC 3 cut(s) 1309, 1423, 1696
GsuI CTGGAG 2 cut(s) 774, 1485
HaeIII GGCC 3 cut(s) 79, 100, 844
HapII CCGG 1 cut(s) 1615
HgaI GACGC 3 cut(s) 929, 1770, 1793
HincII GTYRAC 1 cut(s) 1808
HindII GTYRAC 1 cut(s) 1808
HindIII AAGCTT 1 cut(s) 679
HinfI GANTC 9 cut(s) 59, 284, 602, 959, 983, 1070, 1264, 1550, 1573
HpaII CCGG 1 cut(s) 1615
HphI GGTGA 2 cut(s) 75, 1422
Hpy166II GTNNAC 3 cut(s) 1263, 1639, 1808
Hpy188I TCNGA 7 cut(s) 258, 342, 354, 631, 649, 1350, 1590
Hpy188III TCNNGA 5 cut(s) 1139, 1268, 1301, 1464, 1615
Hpy8I GTNNAC 3 cut(s) 1263, 1639, 1808
HpyAV CCTTC 9 cut(s) 90, 213, 222, 337, 709, 1193, 1298, 1443, 1612
HpyCH4III ACNGT 2 cut(s) 297, 839
HpyCH4V TGCA 5 cut(s) 155, 888, 1229, 1661, 1695
HpyF10VI GCNNNNNNNGC 2 cut(s) 1367, 1704
HpyF3I CTNAG 7 cut(s) 255, 630, 648, 1001, 1398, 1552, 1757
Kpn2I TCCGGA 1 cut(s) 1614
Ksp22I TGATCA 4 cut(s) 337, 421, 484, 547
KspI CCGCGG 2 cut(s) 1212, 1423
Kzo9I GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
LmnI GCTCC 4 cut(s) 439, 502, 793, 1451
Lsp1109I GCAGC 2 cut(s) 1320, 1707
LweI GCATC 2 cut(s) 1216, 1612
MabI ACCWGGT 1 cut(s) 1711
MaeI CTAG 3 cut(s) 53, 75, 944
MaeIII GTNAC 3 cut(s) 854, 1253, 1292
MalI GATC 7 cut(s) 339, 423, 486, 549, 1168, 1395, 1544
MboI GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 3 cut(s) 1166, 1393, 1542
MhlI GDGCHC 2 cut(s) 436, 499
MluCI AATT 7 cut(s) 132, 162, 769, 1006, 1099, 1127, 1325
MlyI GAGTC 7 cut(s) 68, 596, 992, 1064, 1258, 1544, 1567
MmeI TCCRAC 2 cut(s) 882, 1305
Mph1103I ATGCAT 1 cut(s) 157
MroI TCCGGA 1 cut(s) 1614
MroXI GAANNNNTTC 1 cut(s) 1182
MseI TTAA 4 cut(s) 363, 537, 1196, 1655
MslI CAYNNNNRTG 6 cut(s) 429, 492, 518, 888, 1317, 1601
MspA1I CMGCKG 2 cut(s) 1211, 1422
MspI CCGG 1 cut(s) 1615
MspR9I CCNGG 3 cut(s) 792, 1091, 1713
MunI CAATTG 1 cut(s) 162
Mva1269I GAATGC 3 cut(s) 155, 676, 1650
MvaI CCWGG 3 cut(s) 792, 1091, 1713
MvnI CGCG 2 cut(s) 1211, 1422
MwoI GCNNNNNNNGC 2 cut(s) 1367, 1704
NcoI CCATGG 2 cut(s) 137, 519
NdeII GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
NlaIV GGNNCC 5 cut(s) 219, 391, 744, 1168, 1706
NmuCI GTSAC 1 cut(s) 1253
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 180
PagI TCATGA 1 cut(s) 1267
PceI AGGCCT 3 cut(s) 79, 100, 844
PciI ACATGT 1 cut(s) 176
PctI GAATGC 3 cut(s) 155, 676, 1650
PdmI GAANNNNTTC 1 cut(s) 1182
PfeI GAWTC 2 cut(s) 284, 959
PfoI TCCNGGA 2 cut(s) 790, 1089
PkrI GCNGC 3 cut(s) 1310, 1424, 1697
PleI GAGTC 7 cut(s) 67, 596, 991, 1064, 1258, 1544, 1567
PpsI GAGTC 7 cut(s) 67, 596, 991, 1064, 1258, 1544, 1567
PscI ACATGT 1 cut(s) 176
PsiI TTATAA 1 cut(s) 1098
Psp6I CCWGG 3 cut(s) 790, 1089, 1711
PspGI CCWGG 3 cut(s) 790, 1089, 1711
PspN4I GGNNCC 5 cut(s) 219, 391, 744, 1168, 1706
PsrI GAACNNNNNNTAC 2 cut(s) 24, 56
PstI CTGCAG 1 cut(s) 1663
PstNI CAGNNNCTG 2 cut(s) 1505, 1701
PsuI RGATCY 3 cut(s) 1166, 1393, 1542
RsaI GTAC 5 cut(s) 148, 181, 559, 866, 1063
RsaNI GTAC 5 cut(s) 147, 180, 558, 865, 1062
RseI CAYNNNNRTG 6 cut(s) 429, 492, 518, 888, 1317, 1601
SacII CCGCGG 2 cut(s) 1212, 1423
SaqAI TTAA 4 cut(s) 363, 537, 1196, 1655
SatI GCNGC 3 cut(s) 1309, 1423, 1696
Sau3AI GATC 7 cut(s) 337, 421, 484, 547, 1166, 1393, 1542
SchI GAGTC 7 cut(s) 68, 596, 992, 1064, 1258, 1544, 1567
ScrFI CCNGG 3 cut(s) 792, 1091, 1713
SduI GDGCHC 2 cut(s) 436, 499
SetI ASST 8 cut(s) 39, 251, 683, 902, 1255, 1435, 1714, 1740
SexAI ACCWGGT 1 cut(s) 1711
SfaNI GCATC 2 cut(s) 1216, 1612
SfcI CTRYAG 2 cut(s) 1497, 1659
Sfr303I CCGCGG 2 cut(s) 1212, 1423
SgrBI CCGCGG 2 cut(s) 1212, 1423
SmiMI CAYNNNNRTG 6 cut(s) 429, 492, 518, 888, 1317, 1601
Sse9I AATT 7 cut(s) 132, 162, 769, 1006, 1099, 1127, 1325
SseBI AGGCCT 3 cut(s) 79, 100, 844
SsiI CCGC 5 cut(s) 5, 1209, 1211, 1420, 1422
SspI AATATT 1 cut(s) 1523
SspMI CTAG 3 cut(s) 53, 75, 944
StuI AGGCCT 3 cut(s) 79, 100, 844
StyD4I CCNGG 3 cut(s) 790, 1089, 1711
StyI CCWWGG 5 cut(s) 52, 74, 137, 519, 896
TaaI ACNGT 2 cut(s) 297, 839
TaqI TCGA 2 cut(s) 62, 822
TasI AATT 7 cut(s) 132, 162, 769, 1006, 1099, 1127, 1325
TatI WGTACW 2 cut(s) 146, 864
TauI GCSGC 1 cut(s) 1425
TfiI GAWTC 2 cut(s) 284, 959
Tru1I TTAA 4 cut(s) 363, 537, 1196, 1655
Tru9I TTAA 4 cut(s) 363, 537, 1196, 1655
TscAI CASTG 4 cut(s) 1017, 1262, 1560, 1706
TseFI GTSAC 1 cut(s) 1253
TseI GCWGC 2 cut(s) 1308, 1695
Tsp45I GTSAC 1 cut(s) 1253
TspDTI ATGAA 5 cut(s) 160, 674, 1050, 1072, 1338
TspGWI ACGGA 2 cut(s) 1049, 1527
TspRI CASTG 4 cut(s) 1017, 1262, 1560, 1706
XagI CCTNNNNNAGG 1 cut(s) 73
XapI RAATTY 2 cut(s) 132, 1006
XceI RCATGY 1 cut(s) 180
XcmI CCANNNNNNNNNTGG 2 cut(s) 270, 400
XmaJI CCTAGG 2 cut(s) 52, 74
XmnI GAANNNNTTC 1 cut(s) 1182
XspI CTAG 3 cut(s) 53, 75, 944
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.